LOCUS       NM_001009290             587 bp    mRNA    linear   ROD 20-FEB-2025
DEFINITION  Rattus norvegicus NADH:ubiquinone oxidoreductase subunit C2
            (Ndufc2), mRNA.
ACCESSION   NM_001009290 XM_214989
VERSION     NM_001009290.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 587)
  AUTHORS   Raffa,S., Scrofani,C., Valente,S., Micaloni,A., Forte,M.,
            Bianchi,F., Coluccia,R., Geurts,A.M., Sciarretta,S., Volpe,M.,
            Torrisi,M.R. and Rubattu,S.
  TITLE     In vitro characterization of mitochondrial function and structure
            in rat and human cells with a deficiency of the NADH: ubiquinone
            oxidoreductase Ndufc2 subunit
  JOURNAL   Hum Mol Genet 26 (23), 4541-4555 (2017)
   PUBMED   28973657
  REMARK    GeneRIF: Authors found that both fibroblasts obtained from skin of
            heterozygous Ndufc2 knock-out rat model showed marked mitochondrial
            dysfunction and human PBMC homozygous for the TT genotype of the
            rs11237379/NDUFC2 variant, previously shown to associate with
            reduced gene expression, demonstrated increased generation of
            reactive oxygen species and mitochondrial damage.
REFERENCE   3  (bases 1 to 587)
  AUTHORS   Guo,R., Zong,S., Wu,M., Gu,J. and Yang,M.
  TITLE     Architecture of Human Mitochondrial Respiratory Megacomplex
            I2III2IV2
  JOURNAL   Cell 170 (6), 1247-1257 (2017)
   PUBMED   28844695
REFERENCE   4  (bases 1 to 587)
  AUTHORS   Stroud,D.A., Surgenor,E.E., Formosa,L.E., Reljic,B., Frazier,A.E.,
            Dibley,M.G., Osellame,L.D., Stait,T., Beilharz,T.H., Thorburn,D.R.,
            Salim,A. and Ryan,M.T.
  TITLE     Accessory subunits are integral for assembly and function of human
            mitochondrial complex I
  JOURNAL   Nature 538 (7623), 123-126 (2016)
   PUBMED   27626371
REFERENCE   5  (bases 1 to 587)
  AUTHORS   Rubattu,S., Di Castro,S., Schulz,H., Geurts,A.M., Cotugno,M.,
            Bianchi,F., Maatz,H., Hummel,O., Falak,S., Stanzione,R.,
            Marchitti,S., Scarpino,S., Giusti,B., Kura,A., Gensini,G.F.,
            Peyvandi,F., Mannucci,P.M., Rasura,M., Sciarretta,S., Dwinell,M.R.,
            Hubner,N. and Volpe,M.
  TITLE     Ndufc2 Gene Inhibition Is Associated With Mitochondrial Dysfunction
            and Increased Stroke Susceptibility in an Animal Model of Complex
            Human Disease
  JOURNAL   J Am Heart Assoc 5 (2), e002701 (2016)
   PUBMED   26888427
  REMARK    GeneRIF: A significant reduction of Ndufc2 expression causes
            complex I dysfunction and contributes to stroke susceptibility in
            stroke-prone spontaneously hypertensive rats.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 587)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   7  (bases 1 to 587)
  AUTHORS   Murray,J., Zhang,B., Taylor,S.W., Oglesbee,D., Fahy,E.,
            Marusich,M.F., Ghosh,S.S. and Capaldi,R.A.
  TITLE     The subunit composition of the human NADH dehydrogenase obtained by
            rapid one-step immunopurification
  JOURNAL   J Biol Chem 278 (16), 13619-13622 (2003)
   PUBMED   12611891
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC086952.1.
            
            On Jan 7, 2005 this sequence version replaced XM_214989.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC086952.1, DY472315.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q32"
     gene            1..587
                     /gene="Ndufc2"
                     /note="NADH:ubiquinone oxidoreductase subunit C2"
                     /db_xref="GeneID:293130"
                     /db_xref="RGD:1307511"
     exon            1..241
                     /gene="Ndufc2"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..435
                     /gene="Ndufc2"
                     /EC_number="7.1.1.2"
                     /note="NADH dehydrogenase (ubiquinone) 1, subcomplex
                     unknown, 2, 14.5kDa"
                     /codon_start=1
                     /product="NADH dehydrogenase [ubiquinone] 1 subunit C2"
                     /protein_id="NP_001009290.1"
                     /db_xref="GeneID:293130"
                     /db_xref="RGD:1307511"
                     /translation="MMNGRPGHEPLRFLPDEARRLPPPKLNDPRLVYIGFLGYCTGLM
                     DNMMRMRPVMKAGLHRQLLYVTSFFFAGYFYLKRQNYLYAVRDHDMFGYIKLHPEDFP
                     EKEKKTYAEILEPFHPVR"
     exon            242..385
                     /gene="Ndufc2"
                     /inference="alignment:Splign:2.1.0"
     exon            386..561
                     /gene="Ndufc2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgcggggttt gtagtcgtgg tttttcttat agttggagtc gaggtcagac ctcacgtgga
       61 acccgcatca atatgatgaa cggccggccg ggccatgagc ccttaagatt cttgccggat
      121 gaggcccgga gactgccccc gcccaagctg aacgacccgc ggcttgtcta catcggcttc
      181 ctgggctact gcacgggcct gatggacaac atgatgcgaa tgagacctgt gatgaaagca
      241 ggtttgcatc gccagcttct gtatgttact tcctttttct ttgctggata tttttattta
      301 aaacgtcaaa actatctgta tgctgtgagg gaccatgaca tgttcgggta tataaaatta
      361 catccagaag attttcctga gaaagagaag aaaacttatg ctgaaattct tgagccattc
      421 catccagtgc gttgaagtct tcaaaaggct tgatcttcct caccacaacc tgaatatttt
      481 ctgaatttta tgggatgtct ttctgtgaca gtgaagttta ggttaatatg ttaacacctg
      541 taattaaaat agttattatg aaaaaaaaaa aaaaaaaaaa aaaaaaa
//
