LOCUS       NM_001395148             826 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus NADH:ubiquinone oxidoreductase subunit A8
            (Ndufa8), mRNA.
ACCESSION   NM_001395148 XM_008761741
VERSION     NM_001395148.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Guo,R., Zong,S., Wu,M., Gu,J. and Yang,M.
  TITLE     Architecture of Human Mitochondrial Respiratory Megacomplex
            I2III2IV2
  JOURNAL   Cell 170 (6), 1247-1257 (2017)
   PUBMED   28844695
REFERENCE   2  (bases 1 to 826)
  AUTHORS   Stroud,D.A., Surgenor,E.E., Formosa,L.E., Reljic,B., Frazier,A.E.,
            Dibley,M.G., Osellame,L.D., Stait,T., Beilharz,T.H., Thorburn,D.R.,
            Salim,A. and Ryan,M.T.
  TITLE     Accessory subunits are integral for assembly and function of human
            mitochondrial complex I
  JOURNAL   Nature 538 (7623), 123-126 (2016)
   PUBMED   27626371
REFERENCE   3  (bases 1 to 826)
  AUTHORS   Fischer,M., Horn,S., Belkacemi,A., Kojer,K., Petrungaro,C.,
            Habich,M., Ali,M., Kuttner,V., Bien,M., Kauff,F., Dengjel,J.,
            Herrmann,J.M. and Riemer,J.
  TITLE     Protein import and oxidative folding in the mitochondrial
            intermembrane space of intact mammalian cells
  JOURNAL   Mol Biol Cell 24 (14), 2160-2170 (2013)
   PUBMED   23676665
REFERENCE   4  (bases 1 to 826)
  AUTHORS   Ahmed,S.M., Theriault,B.L., Uppalapati,M., Chiu,C.W., Gallie,B.L.,
            Sidhu,S.S. and Angers,S.
  TITLE     KIF14 negatively regulates Rap1a-Radil signaling during breast
            cancer progression
  JOURNAL   J Cell Biol 199 (6), 951-967 (2012)
   PUBMED   23209302
REFERENCE   5  (bases 1 to 826)
  AUTHORS   Szklarczyk,R., Wanschers,B.F., Nabuurs,S.B., Nouws,J.,
            Nijtmans,L.G. and Huynen,M.A.
  TITLE     NDUFB7 and NDUFA8 are located at the intermembrane surface of
            complex I
  JOURNAL   FEBS Lett 585 (5), 737-743 (2011)
   PUBMED   21310150
REFERENCE   6  (bases 1 to 826)
  AUTHORS   Lau,N.C., Seto,A.G., Kim,J., Kuramochi-Miyagawa,S., Nakano,T.,
            Bartel,D.P. and Kingston,R.E.
  TITLE     Characterization of the piRNA complex from rat testes
  JOURNAL   Science 313 (5785), 363-367 (2006)
   PUBMED   16778019
REFERENCE   7  (bases 1 to 826)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   8  (bases 1 to 826)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   9  (bases 1 to 826)
  AUTHORS   Murray,J., Zhang,B., Taylor,S.W., Oglesbee,D., Fahy,E.,
            Marusich,M.F., Ghosh,S.S. and Capaldi,R.A.
  TITLE     The subunit composition of the human NADH dehydrogenase obtained by
            rapid one-step immunopurification
  JOURNAL   J Biol Chem 278 (16), 13619-13622 (2003)
   PUBMED   12611891
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Jun 4, 2021 this sequence version replaced XM_008761741.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DV725525.1, BQ199754.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840115, SAMN16676806
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-177               JAXUCZ010000003.1  39799331-39799507   c
            178-341             JAXUCZ010000003.1  39794169-39794332   c
            342-507             JAXUCZ010000003.1  39786897-39787062   c
            508-826             JAXUCZ010000003.1  39783479-39783797   c
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3p11"
     gene            1..826
                     /gene="Ndufa8"
                     /gene_synonym="Aa2-258"
                     /note="NADH:ubiquinone oxidoreductase subunit A8"
                     /db_xref="GeneID:296658"
                     /db_xref="RGD:1306283"
     exon            1..177
                     /gene="Ndufa8"
                     /gene_synonym="Aa2-258"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..645
                     /gene="Ndufa8"
                     /gene_synonym="Aa2-258"
                     /note="NADH dehydrogenase (ubiquinone) 1 alpha subcomplex,
                     8"
                     /codon_start=1
                     /product="NADH dehydrogenase [ubiquinone] 1 alpha
                     subcomplex subunit 8"
                     /protein_id="NP_001382077.1"
                     /db_xref="GeneID:296658"
                     /db_xref="RGD:1306283"
                     /translation="MPGIVELPTLEELKVEEVKVSSAVLKAAAHHYGAQCDKTNKEFM
                     LCRWEEKDPRRCLKEGKLVNGCALNFFRQIKSHCAEPFTEYWTCLDYSSMQLFRHCRK
                     QQAKFDECVLDKLGWVRPDLGELSKVTKVKTDRPLPENPYHSRPRPEPSPVIEGDLKP
                     AKHGSRFFFWNV"
     exon            178..341
                     /gene="Ndufa8"
                     /gene_synonym="Aa2-258"
                     /inference="alignment:Splign:2.1.0"
     exon            342..507
                     /gene="Ndufa8"
                     /gene_synonym="Aa2-258"
                     /inference="alignment:Splign:2.1.0"
     exon            508..826
                     /gene="Ndufa8"
                     /gene_synonym="Aa2-258"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgcatgcgc ctcgccggag acgcatgcgc agatggtatt gggcagtctg agaaagccaa
       61 aggggaattg aaggagacgg gcgacactgc cgagggcacc tggtcagggt cgcggctgcc
      121 gccgtcatgc cggggatagt ggagctgcca actctggaag agctgaaagt agaggaggtg
      181 aaagtcagct cagccgtgct taaagctgcc gcccatcact atggggctca gtgcgataag
      241 accaataagg agttcatgct gtgccgctgg gaagagaagg accctaggcg ctgcctgaag
      301 gagggcaagc tggtcaacgg ctgcgcactg aacttcttcc ggcagatcaa gagtcactgt
      361 gcggagcctt tcacagagta ctggacttgc cttgattatt ccagcatgca gctgtttcgt
      421 cactgccgca aacagcaggc aaagtttgac gaatgtgtgt tggacaaact gggctgggtg
      481 aggcctgacc ttggggagtt gtctaaggtc accaaagtga aaacagatcg ccctttgcca
      541 gagaatcctt atcactcaag accaaggcca gagcccagcc ccgtgatcga aggggatctg
      601 aagcctgcca agcacggcag ccgctttttc ttctggaacg tgtaaagatg gatccacggc
      661 ccatactcgg ttacctcggt catgcaccca gacaacagac aacgaaaaca cccatgccga
      721 cctagtgttc tttcctggat cacagacgtt aacgaaaaag ttaatttatg tggcttggca
      781 gttactctgt acgtttcctg tccattaaaa atgttaaagg aaacag
//
