LOCUS       NM_001105740            3897 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus N-deacetylase and N-sulfotransferase 2 (Ndst2),
            mRNA.
ACCESSION   NM_001105740 XM_001064811 XM_223793
VERSION     NM_001105740.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Lundequist,A., Tchougounova,E., Abrink,M. and Pejler,G.
  TITLE     Cooperation between mast cell carboxypeptidase A and the chymase
            mouse mast cell protease 4 in the formation and degradation of
            angiotensin II
  JOURNAL   J Biol Chem 279 (31), 32339-32344 (2004)
   PUBMED   15173164
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Aikawa,J., Grobe,K., Tsujimoto,M. and Esko,J.D.
  TITLE     Multiple isozymes of heparan sulfate/heparin GlcNAc
            N-deacetylase/GlcN N-sulfotransferase. Structure and activity of
            the fourth member, NDST4
  JOURNAL   J Biol Chem 276 (8), 5876-5882 (2001)
   PUBMED   11087757
REFERENCE   3  (bases 1 to 3897)
  AUTHORS   McCormick,C., Duncan,G., Goutsos,K.T. and Tufaro,F.
  TITLE     The putative tumor suppressors EXT1 and EXT2 form a stable complex
            that accumulates in the Golgi apparatus and catalyzes the synthesis
            of heparan sulfate
  JOURNAL   Proc Natl Acad Sci U S A 97 (2), 668-673 (2000)
   PUBMED   10639137
REFERENCE   4  (bases 1 to 3897)
  AUTHORS   Forsberg,E., Pejler,G., Ringvall,M., Lunderius,C.,
            Tomasini-Johansson,B., Kusche-Gullberg,M., Eriksson,I., Ledin,J.,
            Hellman,L. and Kjellen,L.
  TITLE     Abnormal mast cells in mice deficient in a heparin-synthesizing
            enzyme
  JOURNAL   Nature 400 (6746), 773-776 (1999)
   PUBMED   10466727
REFERENCE   5  (bases 1 to 3897)
  AUTHORS   Humphries,D.E., Wong,G.W., Friend,D.S., Gurish,M.F., Qiu,W.T.,
            Huang,C., Sharpe,A.H. and Stevens,R.L.
  TITLE     Heparin is essential for the storage of specific granule proteases
            in mast cells
  JOURNAL   Nature 400 (6746), 769-772 (1999)
   PUBMED   10466726
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1.
            
            On Aug 2, 2021 this sequence version replaced NM_001105740.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643297.44591.1,
                                           SRR26643287.31140.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-191               JAXUCZ010000015.1  3612775-3612965
            192-270             JAXUCZ010000015.1  3613546-3613624
            271-1592            JAXUCZ010000015.1  3616453-3617774
            1593-1680           JAXUCZ010000015.1  3617967-3618054
            1681-1835           JAXUCZ010000015.1  3618328-3618482
            1836-2021           JAXUCZ010000015.1  3618678-3618863
            2022-2150           JAXUCZ010000015.1  3619328-3619456
            2151-2333           JAXUCZ010000015.1  3619584-3619766
            2334-2430           JAXUCZ010000015.1  3619853-3619949
            2431-2554           JAXUCZ010000015.1  3620950-3621073
            2555-2729           JAXUCZ010000015.1  3621199-3621373
            2730-2900           JAXUCZ010000015.1  3621538-3621708
            2901-3010           JAXUCZ010000015.1  3621796-3621905
            3011-3113           JAXUCZ010000015.1  3622071-3622173
            3114-3897           JAXUCZ010000015.1  3622278-3623061
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p16"
     gene            1..3897
                     /gene="Ndst2"
                     /note="N-deacetylase and N-sulfotransferase 2"
                     /db_xref="GeneID:114002"
                     /db_xref="RGD:1304700"
     exon            1..191
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     exon            192..270
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     exon            271..1592
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     CDS             588..3239
                     /gene="Ndst2"
                     /EC_number="2.8.2.8"
                     /note="N-deacetylase/N-sulfotransferase (heparan
                     glucosaminyl) 2; N-deacetylase/N-sulfotransferase 2"
                     /codon_start=1
                     /product="bifunctional heparan sulfate
                     N-deacetylase/N-sulfotransferase 2"
                     /protein_id="NP_001099210.1"
                     /db_xref="GeneID:114002"
                     /db_xref="RGD:1304700"
                     /translation="MLQLWKVVRPARQLELHRLILLLIGFSLVSMAFLAYFVSTSPKA
                     KEPLPLPLGDCSSSGAAGPGPARPPVPPRPPRPPETTRTEPVVLVFVESAYSQLGQEI
                     VAILESSRFRYSTELAPGRGDMPTLTDHTHGRYVLVIYENLLKYVNLDSWSRELLDRY
                     CVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLYSNLGLRDYQVNPSAPLLHLTRPSRLE
                     PGPLPGDDWTIFQSNHSTYEPVLIASHRPAELPLPGPALRRARLPTVVQDLGLHDGIQ
                     RVLFGHGLSFWLHKLVFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADV
                     EALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLRHRREFWWFPHMWSH
                     MQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVW
                     GIQVTSTEEYPHLRPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSRELDRSI
                     RGGELFLTVLLNPISVFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTLPPVPLA
                     QKYFELFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLS
                     LHPAVTSSFPSPSTFEEIQFFNGPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDS
                     EVVPRRGAALLPRAKIITVLINPADRAYSWYQHQRAHGDPVALNYTFYQVISASSHAP
                     LLLRSLQSRCLVPGYYSTHLQRWLTYYPSGQLLIMDGQELRVNPAASMEIIQKFLGIT
                     PFLNYTRTLRFDADKGFWCQGLEGGKTRCLGRSKGRRYPDMDMESRLFLTDFFRTHNL
                     ELSKLLSRLGQPAPLWLQEELQHSSVG"
     exon            1593..1680
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1835
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..2021
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2150
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..2333
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     exon            2334..2430
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     exon            2431..2554
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     exon            2555..2729
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     exon            2730..2900
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     exon            2901..3010
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     exon            3011..3113
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
     exon            3114..3897
                     /gene="Ndst2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgggcacccc gagccgcagc gcgccgcctc cccctggtgg gagcagtact tgggcccgac
       61 tgcggcggag cgttcgctcg gagatggcgg agccgccgcg acgcggcccg aggccccgcg
      121 agcagggaag ccgggcggcc ccgggacgcg gcggggcccc gggacagcgg ggacgccgcc
      181 cactccaccg gatatgaagc taaaatgcag tggttacaac acaggagttt gacaggcctg
      241 acctttgaat cccagttcat ccactaacgg attgtaaggg ccgagcgtgt ggacttcact
      301 gactcctcat gtaagaagct acatcggggc tgaggaaaat caagtttcag cctgaggcat
      361 gggccagggg gccatggtga cctgagacca gtagattctg tccaggacac cctgttgctc
      421 tttctgcctc ccctgtcctg tactgaaagc gtttgtgttc atctcatcag ggaactgacc
      481 ctaagaggat ccttcccctg ccaagacagt tccagcccac agagctcata cttcccatct
      541 cctgaggtgg tccctgtccc tctgtccctc cctcccttct cctcgccatg ctccagctgt
      601 ggaaggtggt acgccctgct cggcaactgg aactgcaccg cctcatactg ctgctgattg
      661 gtttcagtct tgtctccatg gcattcctgg cttactttgt atccaccagc cccaaggcca
      721 aggaaccctt gcctctgccc ttgggagact gtagtagcag tggggcagct ggccctggtc
      781 ctgcaaggcc accagtccca cctcgacccc caaggcctcc agaaacaact cgaacagaac
      841 ctgtggtcct tgtgtttgtg gaaagtgcgt actcacaact ggggcaggag attgtggcca
      901 tcttggagtc tagtcgcttt cgttacagca ctgagttagc acctggccga ggggatatgc
      961 ccacgctgac tgatcacacc catggtcgct acgtgttggt catctatgag aaccttctaa
     1021 agtacgtcaa cttggactcc tggagtcgag aactgctaga ccgatactgt gtggagtacg
     1081 gtgtgggcat cattggcttt ttccgagccc acgagcatag cctcctgagt gcccaactca
     1141 agggcttccc ccttttccta tactcaaact tggggctgcg ggactaccaa gtgaaccctt
     1201 ccgctcctct gctacatctc acccgcccta gccgcttgga gccaggacca ctgcctgggg
     1261 acgactggac catcttccag tccaatcaca gcacctatga gccagtgctc atcgccagcc
     1321 atcggccagc agagctcccc ttgccgggac cagctctgcg gcgagcccgg ctccccactg
     1381 tggtgcagga cttggggctt catgacggca tccagcgggt gctgtttgga cacggcctct
     1441 ctttttggct tcacaaactt gtgtttgtcg atgctgtcgc atacctcact ggcaagcgtc
     1501 tctgcctgga ccttgaccgc tacatcttag tagacatcga cgacatcttt gtgggcaaag
     1561 aaggtactcg catgaaggtg gctgatgttg aggctctgct gactacccag aacaaactca
     1621 ggaccttagt ccccaacttc accttcaacc tgggcttctc gggcaagttc tatcatacgg
     1681 ggacagagga ggaggacgcg ggggacgata tgctgctgag gcaccgcaga gagttctggt
     1741 ggttccctca catgtggagt cacatgcagc cacacctgtt ccacaatcgc tccgtgctgg
     1801 ccgaccagat gaggctcaac aaacagtttg ctttggagca tgggattcct acggatctgg
     1861 ggtatgctgt ggccccccac cactctggtg tgtaccccat ccacacgcag ctctatgagg
     1921 cctggaaatc cgtgtggggc attcaggtga ccagcactga ggagtatccc catctgcgcc
     1981 ctgctcgcta ccgccgaggc ttcattcaca atggcatcat ggtgctcccc cggcaaacat
     2041 gtggcctctt cactcacacc atcttctata atgagtatcc tggaggttct cgtgaactgg
     2101 atcgaagcat tcgaggtgga gaactctttc tgacagtgct gcttaatccg atcagtgtct
     2161 ttatgaccca tctgtccaac tatgggaacg accggctggg cctgtacacc tttgagagcc
     2221 tggtgcgctt cctccagtgc tggacacggc tgcggctgca gacccttccc cctgtgcctc
     2281 ttgcccagaa gtactttgaa cttttccctc aggaacgaag ccccctttgg cagaatccct
     2341 gtgacgacaa gaggcacaaa gatatctggt ccaaggagaa aacctgtgac cgactcccca
     2401 agttcctcat tgtgggacca cagaagacag gcaccacagc cattcacttc ttcctgagcc
     2461 tgcaccccgc agtgacaagc agtttcccca gccctagcac ctttgaggaa attcagttct
     2521 tcaatggccc caattaccac aagggcatcg actggtatat ggacttcttc cctgttcctt
     2581 ccaatgccag cactgacttc ctgtttgaaa agagtgccac ctactttgac tcagaggttg
     2641 tacctcggcg gggggctgcc cttttgccaa gagccaagat catcactgtg ctcatcaacc
     2701 ctgctgacag ggcctactcc tggtaccagc accagagagc acatggagac cctgttgctc
     2761 tgaactacac cttctaccaa gtgatttcag cttcctccca cgcccctctg ctgcttcgct
     2821 ccttgcagag ccgttgtctt gttcctggct actattctac ccaccttcag cgctggctga
     2881 cttactaccc ctccggacag ttgctgatta tggatgggca ggagcttcgt gtcaacccag
     2941 cagcctcaat ggagatcatc cagaagttcc tgggtatcac cccctttctg aactacacga
     3001 ggaccctcag gttcgatgca gataaaggat tttggtgcca gggccttgaa ggcgggaaga
     3061 ctcgctgtct agggagaagc aaaggccgga ggtacccaga catggacatg gagtctcgcc
     3121 ttttccttac ggattttttc cgaacccata atttggagct atcaaagctg ctgagtcggc
     3181 ttggacagcc agcccccttg tggcttcagg aagaattgca gcattccagt gtgggctgat
     3241 accccagtcc ttccttccat accagcaaaa ggcctgctaa cttcgccgag ggacaagcct
     3301 tagagcacag cccacagggg gattaatgtg gcctggcccc ttacccctct acctcagtgg
     3361 cccctcagga ttggaatggc cgaaagggga gggcatccat ctctttccca tagttttggg
     3421 gcctaataaa ggggctttcc ttggtacccc cctatggtac taggtactag gtacctctga
     3481 cctatgaacc tgggaggtga ggaagagagg gtccagggaa ggggtagaag aatgtattca
     3541 atggttccct cctcatcccc agcagtttac ctgtcaatgc ctacccatga gagggccctt
     3601 tgctgtggcc cctttattca agacagtggg atctacctgt ggcctggcct ccctagtgtc
     3661 attcacattg aatggggatg aggtcagaca gttgctcata gagccatata tgagcccgag
     3721 ctgtgggctg gaaatgtcct taataaacat ccttattttt ctgcttggtc tgcttcgtcg
     3781 cagaggaaaa caaaccaacc aaccctagac tacagcaggt atcagctgtc cttccacagc
     3841 tcttctccac aaggaagaaa gcagagaccc acgagacaga gtaaaaaagg agtttat
//
