LOCUS       NM_174864               2915 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Rattus norvegicus nicastrin (Ncstn), mRNA.
ACCESSION   NM_174864
VERSION     NM_174864.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Dursun,E. and Gezen-Ak,D.
  TITLE     Vitamin D receptor is present on the neuronal plasma membrane and
            is co-localized with amyloid precursor protein, ADAM10 or Nicastrin
  JOURNAL   PLoS One 12 (11), e0188605 (2017)
   PUBMED   29176823
  REMARK    GeneRIF: These results demonstrated the localization of VDR on the
            neuronal plasma membrane and the co-localization of VDR and APP or
            ADAM10 or Nicastrin and limited co-localization of VDR and PS1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Dries,D.R., Zhu,Y., Brooks,M.M., Forero,D.A., Adachi,M., Cenik,B.,
            West,J.M., Han,Y.H., Yu,C., Arbella,J., Nordin,A., Adolfsson,R.,
            Del-Favero,J., Lu,Q.R., Callaerts,P., Birnbaum,S.G. and Yu,G.
  TITLE     Loss of Nicastrin from Oligodendrocytes Results in Hypomyelination
            and Schizophrenia with Compulsive Behavior
  JOURNAL   J Biol Chem 291 (22), 11647-11656 (2016)
   PUBMED   27008863
REFERENCE   3  (bases 1 to 2915)
  AUTHORS   Bai,X.C., Yan,C., Yang,G., Lu,P., Ma,D., Sun,L., Zhou,R.,
            Scheres,S.H.W. and Shi,Y.
  TITLE     An atomic structure of human gamma-secretase
  JOURNAL   Nature 525 (7568), 212-217 (2015)
   PUBMED   26280335
REFERENCE   4  (bases 1 to 2915)
  AUTHORS   Teranishi,Y., Inoue,M., Yamamoto,N.G., Kihara,T., Wiehager,B.,
            Ishikawa,T., Winblad,B., Schedin-Weiss,S., Frykman,S. and
            Tjernberg,L.O.
  TITLE     Proton myo-inositol cotransporter is a novel gamma-secretase
            associated protein that regulates Abeta production without
            affecting Notch cleavage
  JOURNAL   FEBS J 282 (17), 3438-3451 (2015)
   PUBMED   26094765
  REMARK    Review article
REFERENCE   5  (bases 1 to 2915)
  AUTHORS   Kizuka,Y., Kitazume,S., Fujinawa,R., Saito,T., Iwata,N.,
            Saido,T.C., Nakano,M., Yamaguchi,Y., Hashimoto,Y., Staufenbiel,M.,
            Hatsuta,H., Murayama,S., Manya,H., Endo,T. and Taniguchi,N.
  TITLE     An aberrant sugar modification of BACE1 blocks its lysosomal
            targeting in Alzheimer's disease
  JOURNAL   EMBO Mol Med 7 (2), 175-189 (2015)
   PUBMED   25592972
REFERENCE   6  (bases 1 to 2915)
  AUTHORS   Pasternak,S.H., Bagshaw,R.D., Guiral,M., Zhang,S., Ackerley,C.A.,
            Pak,B.J., Callahan,J.W. and Mahuran,D.J.
  TITLE     Presenilin-1, nicastrin, amyloid precursor protein, and
            gamma-secretase activity are co-localized in the lysosomal membrane
  JOURNAL   J Biol Chem 278 (29), 26687-26694 (2003)
   PUBMED   12736250
  REMARK    GeneRIF: experiments establish amyloid precursor protein,
            nicastrin, and presenilin-1 as resident lysosomal membrane proteins
            and indicate that gamma-secretase is a lysosomal protease
REFERENCE   7  (bases 1 to 2915)
  AUTHORS   Chen,F., Tandon,A., Sanjo,N., Gu,Y.J., Hasegawa,H., Arawaka,S.,
            Lee,F.J., Ruan,X., Mastrangelo,P., Erdebil,S., Wang,L.,
            Westaway,D., Mount,H.T., Yankner,B., Fraser,P.E. and St
            George-Hyslop,P.
  TITLE     Presenilin 1 and presenilin 2 have differential effects on the
            stability and maturation of nicastrin in Mammalian brain
  JOURNAL   J Biol Chem 278 (22), 19974-19979 (2003)
   PUBMED   12646573
REFERENCE   8  (bases 1 to 2915)
  AUTHORS   Lee,S.F., Shah,S., Li,H., Yu,C., Han,W. and Yu,G.
  TITLE     Mammalian APH-1 interacts with presenilin and nicastrin and is
            required for intramembrane proteolysis of amyloid-beta precursor
            protein and Notch
  JOURNAL   J Biol Chem 277 (47), 45013-45019 (2002)
   PUBMED   12297508
REFERENCE   9  (bases 1 to 2915)
  AUTHORS   Dermaut,B., Theuns,J., Sleegers,K., Hasegawa,H., Van den Broeck,M.,
            Vennekens,K., Corsmit,E., St George-Hyslop,P., Cruts,M., van
            Duijn,C.M. and Van Broeckhoven,C.
  TITLE     The gene encoding nicastrin, a major gamma-secretase component,
            modifies risk for familial early-onset Alzheimer disease in a Dutch
            population-based sample
  JOURNAL   Am J Hum Genet 70 (6), 1568-1574 (2002)
   PUBMED   11992262
REFERENCE   10 (bases 1 to 2915)
  AUTHORS   Leem,J.Y., Vijayan,S., Han,P., Cai,D., Machura,M., Lopes,K.O.,
            Veselits,M.L., Xu,H. and Thinakaran,G.
  TITLE     Presenilin 1 is required for maturation and cell surface
            accumulation of nicastrin
  JOURNAL   J Biol Chem 277 (21), 19236-19240 (2002)
   PUBMED   11943765
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC074006.1 and AY101378.1.
            
            On Oct 8, 2005 this sequence version replaced NM_174864.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY101378.1, SRR8487231.45426.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-92                BC074006.1         1-92
            93-1795             AY101378.1         1-1703
            1796-2915           BC074006.1         1911-3030
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q24"
     gene            1..2915
                     /gene="Ncstn"
                     /note="nicastrin"
                     /db_xref="GeneID:289231"
                     /db_xref="RGD:631418"
     exon            1..174
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..2219
                     /gene="Ncstn"
                     /codon_start=1
                     /product="nicastrin precursor"
                     /protein_id="NP_777353.1"
                     /db_xref="GeneID:289231"
                     /db_xref="RGD:631418"
                     /translation="MATARGGSGPDPGSRGLLLLSFSVVLAGLCGGNSVERKIYIPLN
                     KTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEDDLKWVLTDGPNPPYMVLLEGKLF
                     TRDIMEKLKGETSRIAGLAVTLAKPNSTSSFSPSVQCPNDGFGIYSNSYGPEFAHCKK
                     TLWNELGNGLAYDDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFS
                     HMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSGGLEPDVRVVV
                     AATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLPRNVMFVFFQGETF
                     DYIGSSRMVYDMENGKFPVRLENIDSFVELGQVALRTSLELWMHTDPMSQKNESVKNQ
                     VEDLLVTLEQSGADTPQVVLSRLVQSQALPPSSLQRFLRARNISGVVLADHSGSFHNR
                     YYQSIYDTAENINVTYPESQSPEEDLNFVTDTAKALADVATVLARALYKLAGGTNFNN
                     SIQADPQTVTRLLYGFLVRANNSWFQSILRHDLRSYLDDGPLQHYIAVSSPTNTTYVV
                     QYALANLTGKVTNLTQEQCQDPSKVPNESKDLYEYSWVQGPWNSNKTERLPRCVRSTV
                     RLARALSPAFELSQWSSTEYSTWAESRWKDIQARIFLIASKELEFITLIVGFSILVFS
                     LIVTYCINAKADVLFVAPREPGAVSY"
     exon            175..279
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            280..403
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            404..525
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            526..671
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            672..822
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            823..932
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1085
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1190
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1268
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1441
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1544
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1640
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1728
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1883
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..2096
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
     exon            2097..2891
                     /gene="Ncstn"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgctgggcca cagagacgat gccagcggaa gcctagagag accgctaaga gactggaagg
       61 gacccgaggc ttccgcccag cggagaggca agatggctac ggctaggggc ggctctgggc
      121 ctgacccagg aagtcggggt ctccttcttc tgtctttttc cgtggtacta gcaggattgt
      181 gtgggggaaa ctcagtggag aggaagatct acattccctt aaataaaaca gctccttgtg
      241 tccgcctgct caatgccact catcagattg gctgccagtc ttcaattagt ggggatacag
      301 gggttatcca cgtagtggag aaagaagacg acctgaaatg ggtattgacg gatggcccca
      361 acccccctta catggttctg ctggagggca agctctttac cagagatata atggaaaagc
      421 tgaaggggga aaccagtaga atcgctggtc ttgccgtgac tctagccaag cccaattcaa
      481 cgtcaagctt ctctcctagc gtgcagtgcc caaatgatgg gtttggtatt tactccaact
      541 cctatgggcc agagtttgct cactgcaaaa aaacattgtg gaatgaactg ggcaatggct
      601 tggcttatga cgacttcagt ttccccatct ttcttcttga agatgagaac gaaaccaagg
      661 tcatcaagca gtgctatcaa gatcacaacc tgggtcagaa tggctctgca ccgagcttcc
      721 cactgtgtgc tatgcagctc ttctcacaca tgcacgccgt catcagcacc gccacctgca
      781 tgcgacgcag cttcatccag agcaccttca gcatcaaccc agaaatcgtc tgtgacccct
      841 tatccgacta caacgtatgg agcatgctta agcctataaa tacgtctggg ggattagaac
      901 ctgatgtcag ggttgtggtt gcagccacac ggctggatag ccggtccttc ttctggaatg
      961 tggccccagg ggcagaaagt gccgttgcct cctttgtcac tcagctggcc gcagctgaag
     1021 ctttgcacaa ggcacctgat gtgaccaccc taccccgaaa tgtgatgttt gtcttcttcc
     1081 agggggaaac ttttgactac attggcagct caaggatggt ctatgacatg gagaacggca
     1141 agtttcctgt gcggctagag aacattgact cctttgtgga gctggggcag gtggccctaa
     1201 ggacgtcact agaactctgg atgcacacag atcctatgtc tcagaaaaat gaatctgtga
     1261 agaaccaggt ggaggatctt ctggtcactc tggagcagag tggtgctgat acccctcaag
     1321 ttgtcctgag cagactagtc cagtcccagg cccttccacc atcatctcta caacgatttc
     1381 ttcgggctcg aaacatctct ggtgtggtct tggctgacca ctctggttcc ttccacaatc
     1441 ggtattacca gagcatttat gacactgctg agaacattaa cgtgacctat cctgagtcgc
     1501 agagcccaga agaggacctc aactttgtaa cagacactgc caaggcactg gcagatgttg
     1561 ccacagtgct ggcacgagct ttgtacaagc ttgcaggagg aaccaacttc aacaactcca
     1621 ttcaggctga tccccagaca gttacacgtc tgctctatgg gttcctggtc agagctaaca
     1681 actcatggtt tcagtctatc ctcagacatg atctgaggtc ctatttggat gatgggcctc
     1741 ttcaacacta catcgctgtc tccagcccta ccaacacgac ttacgttgtg cagtatgcct
     1801 tggcaaacct gactggcaag gtgaccaacc tcacccaaga gcagtgccag gatccaagta
     1861 aagtcccaaa tgaaagcaag gatttgtatg aatactcgtg ggtacaaggc ccttggaatt
     1921 ccaacaagac agagcggctc ccacggtgtg tgcgctccac agtacgactg gctagggcct
     1981 tgtcccccgc ctttgagctg agtcagtgga gctccacaga atactctacg tgggccgaga
     2041 gccgctggaa agatatccaa gctcggatat tcctcattgc cagcaaagaa cttgagttca
     2101 tcacgctgat cgtgggcttc agcatcctcg tcttttctct catcgtcact tattgtatca
     2161 acgccaaggc cgatgtcctt ttcgttgctc cccgagagcc aggagctgtg tcttactgaa
     2221 gaggactcca gctctccctg ccagctctga actttccttc ccaaaccagg tgtctgactg
     2281 ggaacaaacc actaatttgt cactggactg tctctgggcc tgcttagact gggattgaca
     2341 ggtagatgga aatgtgagtg gagagataac taagttgcct ccccttctca gccccccttt
     2401 cccattcgtt tttccttctc taataccagg gattacgaca gaggcctgct gctcctgtct
     2461 aactcccagt taccctctcc catcagccct ttctcgggat tcctcctgtc tttcatcctg
     2521 ccctgtactg ccctctgacc ccactgtcac ccgaccccca cagcctcctg tgttcagcca
     2581 cctgctgcac caggaagagg gtgtgaaaga ttgggcatcg gcatccaagc accctgaacc
     2641 tgagggaagg aatgggttcc ctggctgagc cagtgtctct ttctcactgt cctttctcca
     2701 ggcgtgcaga tggcatgtta gtgtgggcat gctgttaggt gggtatccca cctccagccc
     2761 acagtgctcg gatgtacttt ttattattaa actataatat ctatttttgt tgggcttttt
     2821 cttttttttc ttttttttct tttttttgta aatatataaa tagcaagttt cattaaaatt
     2881 atccaataca aaaaaaaaaa aaaaaaaaaa aaaaa
//
