LOCUS       NP_777353                708 aa            linear   ROD 18-MAR-2025
DEFINITION  nicastrin precursor [Rattus norvegicus].
ACCESSION   NP_777353
VERSION     NP_777353.1
DBSOURCE    REFSEQ: accession NM_174864.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 708)
  AUTHORS   Dursun,E. and Gezen-Ak,D.
  TITLE     Vitamin D receptor is present on the neuronal plasma membrane and
            is co-localized with amyloid precursor protein, ADAM10 or Nicastrin
  JOURNAL   PLoS One 12 (11), e0188605 (2017)
   PUBMED   29176823
  REMARK    GeneRIF: These results demonstrated the localization of VDR on the
            neuronal plasma membrane and the co-localization of VDR and APP or
            ADAM10 or Nicastrin and limited co-localization of VDR and PS1.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 708)
  AUTHORS   Dries,D.R., Zhu,Y., Brooks,M.M., Forero,D.A., Adachi,M., Cenik,B.,
            West,J.M., Han,Y.H., Yu,C., Arbella,J., Nordin,A., Adolfsson,R.,
            Del-Favero,J., Lu,Q.R., Callaerts,P., Birnbaum,S.G. and Yu,G.
  TITLE     Loss of Nicastrin from Oligodendrocytes Results in Hypomyelination
            and Schizophrenia with Compulsive Behavior
  JOURNAL   J Biol Chem 291 (22), 11647-11656 (2016)
   PUBMED   27008863
REFERENCE   3  (residues 1 to 708)
  AUTHORS   Bai,X.C., Yan,C., Yang,G., Lu,P., Ma,D., Sun,L., Zhou,R.,
            Scheres,S.H.W. and Shi,Y.
  TITLE     An atomic structure of human gamma-secretase
  JOURNAL   Nature 525 (7568), 212-217 (2015)
   PUBMED   26280335
REFERENCE   4  (residues 1 to 708)
  AUTHORS   Teranishi,Y., Inoue,M., Yamamoto,N.G., Kihara,T., Wiehager,B.,
            Ishikawa,T., Winblad,B., Schedin-Weiss,S., Frykman,S. and
            Tjernberg,L.O.
  TITLE     Proton myo-inositol cotransporter is a novel gamma-secretase
            associated protein that regulates Abeta production without
            affecting Notch cleavage
  JOURNAL   FEBS J 282 (17), 3438-3451 (2015)
   PUBMED   26094765
  REMARK    Review article
REFERENCE   5  (residues 1 to 708)
  AUTHORS   Kizuka,Y., Kitazume,S., Fujinawa,R., Saito,T., Iwata,N.,
            Saido,T.C., Nakano,M., Yamaguchi,Y., Hashimoto,Y., Staufenbiel,M.,
            Hatsuta,H., Murayama,S., Manya,H., Endo,T. and Taniguchi,N.
  TITLE     An aberrant sugar modification of BACE1 blocks its lysosomal
            targeting in Alzheimer's disease
  JOURNAL   EMBO Mol Med 7 (2), 175-189 (2015)
   PUBMED   25592972
REFERENCE   6  (residues 1 to 708)
  AUTHORS   Pasternak,S.H., Bagshaw,R.D., Guiral,M., Zhang,S., Ackerley,C.A.,
            Pak,B.J., Callahan,J.W. and Mahuran,D.J.
  TITLE     Presenilin-1, nicastrin, amyloid precursor protein, and
            gamma-secretase activity are co-localized in the lysosomal membrane
  JOURNAL   J Biol Chem 278 (29), 26687-26694 (2003)
   PUBMED   12736250
  REMARK    GeneRIF: experiments establish amyloid precursor protein,
            nicastrin, and presenilin-1 as resident lysosomal membrane proteins
            and indicate that gamma-secretase is a lysosomal protease
REFERENCE   7  (residues 1 to 708)
  AUTHORS   Chen,F., Tandon,A., Sanjo,N., Gu,Y.J., Hasegawa,H., Arawaka,S.,
            Lee,F.J., Ruan,X., Mastrangelo,P., Erdebil,S., Wang,L.,
            Westaway,D., Mount,H.T., Yankner,B., Fraser,P.E. and St
            George-Hyslop,P.
  TITLE     Presenilin 1 and presenilin 2 have differential effects on the
            stability and maturation of nicastrin in Mammalian brain
  JOURNAL   J Biol Chem 278 (22), 19974-19979 (2003)
   PUBMED   12646573
REFERENCE   8  (residues 1 to 708)
  AUTHORS   Lee,S.F., Shah,S., Li,H., Yu,C., Han,W. and Yu,G.
  TITLE     Mammalian APH-1 interacts with presenilin and nicastrin and is
            required for intramembrane proteolysis of amyloid-beta precursor
            protein and Notch
  JOURNAL   J Biol Chem 277 (47), 45013-45019 (2002)
   PUBMED   12297508
REFERENCE   9  (residues 1 to 708)
  AUTHORS   Dermaut,B., Theuns,J., Sleegers,K., Hasegawa,H., Van den Broeck,M.,
            Vennekens,K., Corsmit,E., St George-Hyslop,P., Cruts,M., van
            Duijn,C.M. and Van Broeckhoven,C.
  TITLE     The gene encoding nicastrin, a major gamma-secretase component,
            modifies risk for familial early-onset Alzheimer disease in a Dutch
            population-based sample
  JOURNAL   Am J Hum Genet 70 (6), 1568-1574 (2002)
   PUBMED   11992262
REFERENCE   10 (residues 1 to 708)
  AUTHORS   Leem,J.Y., Vijayan,S., Han,P., Cai,D., Machura,M., Lopes,K.O.,
            Veselits,M.L., Xu,H. and Thinakaran,G.
  TITLE     Presenilin 1 is required for maturation and cell surface
            accumulation of nicastrin
  JOURNAL   J Biol Chem 277 (21), 19236-19240 (2002)
   PUBMED   11943765
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC074006.1 and AY101378.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY101378.1, SRR8487231.45426.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Rattus norvegicus"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q24"
     Protein         1..708
                     /product="nicastrin precursor"
                     /calculated_mol_wt=75200
     sig_peptide     1..34
                     /note="/evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
                     /calculated_mol_wt=3219
     mat_peptide     35..708
                     /product="Nicastrin. /id=PRO_0000019683"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CGU6.1)"
                     /calculated_mol_wt=75200
     Site            44
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Region          51..653
                     /region_name="M28_Nicastrin"
                     /note="M28 Zn-peptidase nicastrin, a main component of
                     gamma-secretase complex; cd03881"
                     /db_xref="CDD:349877"
     Site            54
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            128
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            186
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            203
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            263
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            335..339
                     /site_type="other"
                     /note="DYIGS region"
                     /db_xref="CDD:349877"
     Site            386
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            434
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0007744|PubMed:24090084; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            463
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            507
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            529
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            561
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            572
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            579
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            593
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            611
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8CGU6.1)"
     Site            669..689
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CGU6.1)"
     CDS             1..708
                     /gene="Ncstn"
                     /coded_by="NM_174864.3:93..2219"
                     /db_xref="GeneID:289231"
                     /db_xref="RGD:631418"
ORIGIN      
        1 matarggsgp dpgsrgllll sfsvvlaglc ggnsverkiy iplnktapcv rllnathqig
       61 cqssisgdtg vihvvekedd lkwvltdgpn ppymvllegk lftrdimekl kgetsriagl
      121 avtlakpnst ssfspsvqcp ndgfgiysns ygpefahckk tlwnelgngl ayddfsfpif
      181 lledenetkv ikqcyqdhnl gqngsapsfp lcamqlfshm havistatcm rrsfiqstfs
      241 inpeivcdpl sdynvwsmlk pintsgglep dvrvvvaatr ldsrsffwnv apgaesavas
      301 fvtqlaaaea lhkapdvttl prnvmfvffq getfdyigss rmvydmengk fpvrlenids
      361 fvelgqvalr tslelwmhtd pmsqknesvk nqvedllvtl eqsgadtpqv vlsrlvqsqa
      421 lppsslqrfl rarnisgvvl adhsgsfhnr yyqsiydtae ninvtypesq speedlnfvt
      481 dtakaladva tvlaralykl aggtnfnnsi qadpqtvtrl lygflvrann swfqsilrhd
      541 lrsylddgpl qhyiavsspt nttyvvqyal anltgkvtnl tqeqcqdpsk vpneskdlye
      601 yswvqgpwns nkterlprcv rstvrlaral spafelsqws steystwaes rwkdiqarif
      661 liaskelefi tlivgfsilv fslivtycin akadvlfvap repgavsy
//
