LOCUS       NM_024366               1768 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus neuronal calcium sensor 1 (Ncs1), mRNA.
ACCESSION   NM_024366
VERSION     NM_024366.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Gohain,D., Deka,R. and Tamuli,R.
  TITLE     Identification of critical amino acid residues and functional
            conservation of the Neurospora crassa and Rattus norvegicus
            orthologues of neuronal calcium sensor-1
  JOURNAL   Genetica 144 (6), 665-674 (2016)
   PUBMED   27796528
  REMARK    GeneRIF: three critical amino acid residues of N. crassa NCS-1 and
            its functional conservation across species using the orthologue
            from R. norvegicus
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Pandalaneni,S., Karuppiah,V., Saleem,M., Haynes,L.P.,
            Burgoyne,R.D., Mayans,O., Derrick,J.P. and Lian,L.Y.
  TITLE     Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and
            G-protein-coupled Receptor Kinase 1 (GRK1) Peptides Using Different
            Modes of Interactions
  JOURNAL   J Biol Chem 290 (30), 18744-18756 (2015)
   PUBMED   25979333
  REMARK    GeneRIF: In the Ca(2+)/NCS-1.D2R peptide complex, the C-terminal
            region adopts a 310 helix-turn-310 helix, whereas in the GRK1
            peptide complex it forms an a-helix
REFERENCE   3  (bases 1 to 1768)
  AUTHORS   Comim,C.M., Silva,N.C., Mina,F., Dominguini,D., Scaini,G.,
            Morais,M.O., Rosa,D.V., Magno,L.A., Streck,E.L., Romano-Silva,M.A.,
            Quevedo,J. and Dal-Pizzol,F.
  TITLE     Evaluation of NCS-1, DARPP-32, and neurotrophins in hippocampus and
            prefrontal cortex in rats submitted to sepsis
  JOURNAL   Synapse 68 (10), 474-479 (2014)
   PUBMED   24978930
  REMARK    GeneRIF: NCS-1 was decreased in prefrontal cortex 48 hours after
            sepsis induction
REFERENCE   4  (bases 1 to 1768)
  AUTHORS   Drumond,L.E., Mourao,F.A., Leite,H.R., Abreu,R.V., Reis,H.J.,
            Moraes,M.F., Pereira,G.S. and Massensini,A.R.
  TITLE     Differential effects of swimming training on neuronal calcium
            sensor-1 expression in rat hippocampus/cortex and in object
            recognition memory tasks
  JOURNAL   Brain Res Bull 88 (4), 385-391 (2012)
   PUBMED   22521426
  REMARK    GeneRIF: physical exercise could be modulating NCS-1 in an activity
            dependent manner, for specific neural substrates
REFERENCE   5  (bases 1 to 1768)
  AUTHORS   Chandra,K., Sharma,Y. and Chary,K.V.
  TITLE     Characterization of low-energy excited states in the native state
            ensemble of non-myristoylated and myristoylated neuronal calcium
            sensor-1
  JOURNAL   Biochim Biophys Acta 1814 (2), 334-344 (2011)
   PUBMED   21035569
  REMARK    GeneRIF: characterized individual residues in both
            non-myristoylated and myristoylated NCS-1 that access alternate
            states by measuring nonlinear temperature dependence of the
            backbone amide-proton ((1)H(N)) chemical shifts
REFERENCE   6  (bases 1 to 1768)
  AUTHORS   Chen,C., Yu,L., Zhang,P., Jiang,J., Zhang,Y., Chen,X., Wu,Q., Wu,Q.
            and Zhao,S.
  TITLE     Human neuronal calcium sensor-1 shows the highest expression level
            in cerebral cortex
  JOURNAL   Neurosci Lett 319 (2), 67-70 (2002)
   PUBMED   11825672
  REMARK    GeneRIF: The expression level of NCS-1 in cerebral cortex is the
            highest.NCS-1 in human brain might be involved in a variety of
            brain functions such as sensory processing, motor control,
            emotional control, learning and memory.
REFERENCE   7  (bases 1 to 1768)
  AUTHORS   McFerran,B.W., Weiss,J.L. and Burgoyne,R.D.
  TITLE     Neuronal Ca(2+) sensor 1. Characterization of the myristoylated
            protein, its cellular effects in permeabilized adrenal chromaffin
            cells, Ca(2+)-independent membrane association, and interaction
            with binding proteins, suggesting a role in rapid Ca(2+) signal
            transduction
  JOURNAL   J Biol Chem 274 (42), 30258-30265 (1999)
   PUBMED   10514519
REFERENCE   8  (bases 1 to 1768)
  AUTHORS   McFerran,B.W., Graham,M.E. and Burgoyne,R.D.
  TITLE     Neuronal Ca2+ sensor 1, the mammalian homologue of frequenin, is
            expressed in chromaffin and PC12 cells and regulates neurosecretion
            from dense-core granules
  JOURNAL   J Biol Chem 273 (35), 22768-22772 (1998)
   PUBMED   9712909
REFERENCE   9  (bases 1 to 1768)
  AUTHORS   Olafsson,P., Soares,H.D., Herzog,K.H., Wang,T., Morgan,J.I. and
            Lu,B.
  TITLE     The Ca2+ binding protein, frequenin is a nervous system-specific
            protein in mouse preferentially localized in neurites
  JOURNAL   Brain Res Mol Brain Res 44 (1), 73-82 (1997)
   PUBMED   9030700
REFERENCE   10 (bases 1 to 1768)
  AUTHORS   De Castro,E., Nef,S., Fiumelli,H., Lenz,S.E., Kawamura,S. and
            Nef,P.
  TITLE     Regulation of rhodopsin phosphorylation by a family of neuronal
            calcium sensors
  JOURNAL   Biochem Biophys Res Commun 216 (1), 133-140 (1995)
   PUBMED   7488079
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Nov 26, 2020 this sequence version replaced NM_024366.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X82188.1, SRR26643298.15035.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-124               JAXUCZ010000003.1  34920949-34921072
            125-149             JAXUCZ010000003.1  34947431-34947455
            150-288             JAXUCZ010000003.1  34954908-34955046
            289-367             JAXUCZ010000003.1  34956364-34956442
            368-456             JAXUCZ010000003.1  34958183-34958271
            457-534             JAXUCZ010000003.1  34958645-34958722
            535-651             JAXUCZ010000003.1  34961312-34961428
            652-1768            JAXUCZ010000003.1  34965438-34966554
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3p12"
     gene            1..1768
                     /gene="Ncs1"
                     /gene_synonym="Freq"
                     /note="neuronal calcium sensor 1"
                     /db_xref="GeneID:65153"
                     /db_xref="RGD:68417"
     exon            1..124
                     /gene="Ncs1"
                     /gene_synonym="Freq"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..633
                     /gene="Ncs1"
                     /gene_synonym="Freq"
                     /note="NCS-1; frequenin-like protein; frequenin-like
                     ubiquitous protein"
                     /codon_start=1
                     /product="neuronal calcium sensor 1"
                     /protein_id="NP_077342.1"
                     /db_xref="GeneID:65153"
                     /db_xref="RGD:68417"
                     /translation="MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLD
                     AAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLR
                     WAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNA
                     DGKLTLQEFQEGSKADPSIVQALSLYDGLV"
     exon            125..149
                     /gene="Ncs1"
                     /gene_synonym="Freq"
                     /inference="alignment:Splign:2.1.0"
     exon            150..288
                     /gene="Ncs1"
                     /gene_synonym="Freq"
                     /inference="alignment:Splign:2.1.0"
     exon            289..367
                     /gene="Ncs1"
                     /gene_synonym="Freq"
                     /inference="alignment:Splign:2.1.0"
     exon            368..456
                     /gene="Ncs1"
                     /gene_synonym="Freq"
                     /inference="alignment:Splign:2.1.0"
     exon            457..534
                     /gene="Ncs1"
                     /gene_synonym="Freq"
                     /inference="alignment:Splign:2.1.0"
     exon            535..651
                     /gene="Ncs1"
                     /gene_synonym="Freq"
                     /inference="alignment:Splign:2.1.0"
     exon            652..1768
                     /gene="Ncs1"
                     /gene_synonym="Freq"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctcccgctg ggcgccccga gcgggtccgg cccggggagc gggggccgcg gccgccgagc
       61 atggggaaat ccaacagcaa gttgaagcct gaagttgtgg aggagctgac cagaaaaacc
      121 tacttcactg aaaaggaagt acagcagtgg tacaagggtt tcattaagga ctgccccagt
      181 gggcagctgg acgccgctgg cttccagaag atctacaaac agttcttccc atttggagac
      241 cccaccaagt tcgccacgtt tgttttcaac gtcttcgacg agaacaagga tggcaggatc
      301 gagttctccg aattcatcca ggctctatcg gtgacctcaa gggggaccct ggatgagaag
      361 ttgaggtggg ccttcaagct ttatgacttg gataacgacg gctacatcac cagaaacgag
      421 atgctggaca tagtggatgc catttaccag atggtgggga acaccgtgga gctcccagaa
      481 gaagagaaca caccagagaa gagggtggac cggatcttcg ccatgatgga taagaacgct
      541 gatgggaagc taactcttca ggagttccag gaaggctcca aggccgaccc ctccattgtg
      601 caggcgctgt ccctctacga cgggctggta tagtccaggc ccagagctgt gatgtgtggg
      661 aaccactagc ctcttcctgt gccatgaggc caccgcagcc tgacaccgcc cctgtgccca
      721 cccagccttc ctcgtctcca caaagccggc cgcccttgag cctctaggcc ctggctgtct
      781 ttcccccagc cctgcatcca cctgccgcct gaagccactg gttccatcgt caacgacctc
      841 cgcttgtcca aacaacgaca acccaaaacg gaaaggctcc agccctctgc aaacctgagc
      901 gccgggctgc ctccctcggc agccgtcccg ttcttcctgc tgtctggcct gtgtgctttc
      961 ttttttcttt ttttatttca aatggacaat gtaaaagaaa acaaactacc ttccgttcta
     1021 aaagatactg accattggga gaaggcctct gtgtctcaga gaactcagtc tctgcctggt
     1081 cccctgcagt ggtggccagg ggcagtgagg ccactggcag gcaagggtgt tagcggcagt
     1141 gactcacgtg ataacagtat tgtgcatttt gtggggaaac tgagggtttt ttttttttat
     1201 actcatacat aatcgatacc ttttatttat tggttgttaa ctattgctgc taccttgtgt
     1261 gtctgaaact cccaggatgc tgggcctccg tttacatgtg cacaccctca cccacctgcc
     1321 cgccacactt gggaggatgg tggcctgcag cggccaagaa gccaaaaaaa tcttttcctt
     1381 tttttttttt tttttctttc tttctcagat actgtgctcc gtttttggag aggggaaggt
     1441 aggcattcct cgatggctaa tgcactgttc ccccagcttc tgccctgaag cccccacccc
     1501 tctcctctgc accccttgga gcccataaca ctttttttca agaacacact tggggtgttt
     1561 tttgtttttt ggtttttttt tttttaattt ttgcaacttt actctgatgt ttgcagcaga
     1621 gaaaagatgg agagccttgc tctgggagtg ggaactggga gcgggtgtcg ggcacacaga
     1681 atagagtatg aagtgggctt ctctgggaac tgctggcctc tgcctccttg atgctaacta
     1741 aggggctggg taggtatagc gtgtgaac
//
