LOCUS       NM_053454               4663 bp    mRNA    linear   ROD 07-APR-2024
DEFINITION  Rattus norvegicus nuclear receptor coactivator 3 (Ncoa3), mRNA.
ACCESSION   NM_053454 XM_006224744 XM_006235634
VERSION     NM_053454.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Lan,X., Ma,H., Xiong,Y., Zou,L., Yuan,Z. and Xiao,Y.
  TITLE     Bone marrow mesenchymal stem cells-derived exosomes mediate nuclear
            receptor coactivator-3 expression in osteoblasts by delivering
            miR-532-5p to influence osteonecrosis of the femoral head
            development
  JOURNAL   Cell Biol Int 46 (12), 2185-2197 (2022)
   PUBMED   36116109
  REMARK    GeneRIF: Bone marrow mesenchymal stem cells-derived exosomes
            mediate nuclear receptor coactivator-3 expression in osteoblasts by
            delivering miR-532-5p to influence osteonecrosis of the femoral
            head development.
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Uranishi,K., Akagi,T., Koide,H. and Yokota,T.
  TITLE     Esrrb directly binds to Gata6 promoter and regulates its expression
            with Dax1 and Ncoa3
  JOURNAL   Biochem Biophys Res Commun 478 (4), 1720-1725 (2016)
   PUBMED   27601327
REFERENCE   3  (bases 1 to 4663)
  AUTHORS   Storchel,P.H., Thummler,J., Siegel,G., Aksoy-Aksel,A., Zampa,F.,
            Sumer,S. and Schratt,G.
  TITLE     A large-scale functional screen identifies Nova1 and Ncoa3 as
            regulators of neuronal miRNA function
  JOURNAL   EMBO J 34 (17), 2237-2254 (2015)
   PUBMED   26105073
  REMARK    GeneRIF: Nova1 and Ncoa3 stimulate miRNA function by different
            mechanisms that converge on Argonaute (Ago) proteins, core
            components of the miRNA-induced silencing complex (miRISC).
REFERENCE   4  (bases 1 to 4663)
  AUTHORS   Bhat,M., Noolu,B., Qadri,S.S. and Ismail,A.
  TITLE     Vitamin D deficiency decreases adiposity in rats and causes altered
            expression of uncoupling proteins and steroid receptor coactivator3
  JOURNAL   J Steroid Biochem Mol Biol 144 Pt B, 304-312 (2014)
   PUBMED   25132457
  REMARK    GeneRIF: Vitamin D deficiency decreases adiposity in rats and
            causes altered expression of uncoupling proteins and steroid
            receptor coactivator3
REFERENCE   5  (bases 1 to 4663)
  AUTHORS   Hayes,J.D. and Dinkova-Kostova,A.T.
  TITLE     The Nrf2 regulatory network provides an interface between redox and
            intermediary metabolism
  JOURNAL   Trends Biochem Sci 39 (4), 199-218 (2014)
   PUBMED   24647116
  REMARK    Review article
REFERENCE   6  (bases 1 to 4663)
  AUTHORS   O'Brien,T.W., Liu,J., Sylvester,J.E., Mougey,E.B.,
            Fischel-Ghodsian,N., Thiede,B., Wittmann-Liebold,B. and Graack,H.R.
  TITLE     Mammalian mitochondrial ribosomal proteins (4). Amino acid
            sequencing, characterization, and identification of corresponding
            gene sequences
  JOURNAL   J Biol Chem 275 (24), 18153-18159 (2000)
   PUBMED   10751423
REFERENCE   7  (bases 1 to 4663)
  AUTHORS   Xu,J., Liao,L., Ning,G., Yoshida-Komiya,H., Deng,C. and
            O'Malley,B.W.
  TITLE     The steroid receptor coactivator SRC-3
            (p/CIP/RAC3/AIB1/ACTR/TRAM-1) is required for normal growth,
            puberty, female reproductive function, and mammary gland
            development
  JOURNAL   Proc Natl Acad Sci U S A 97 (12), 6379-6384 (2000)
   PUBMED   10823921
REFERENCE   8  (bases 1 to 4663)
  AUTHORS   Martinez de Arrieta,C., Koibuchi,N. and Chin,W.W.
  TITLE     Coactivator and corepressor gene expression in rat cerebellum
            during postnatal development and the effect of altered thyroid
            status
  JOURNAL   Endocrinology 141 (5), 1693-1698 (2000)
   PUBMED   10803578
REFERENCE   9  (bases 1 to 4663)
  AUTHORS   Chen,H., Lin,R.J., Schiltz,R.L., Chakravarti,D., Nash,A., Nagy,L.,
            Privalsky,M.L., Nakatani,Y. and Evans,R.M.
  TITLE     Nuclear receptor coactivator ACTR is a novel histone
            acetyltransferase and forms a multimeric activation complex with
            P/CAF and CBP/p300
  JOURNAL   Cell 90 (3), 569-580 (1997)
   PUBMED   9267036
REFERENCE   10 (bases 1 to 4663)
  AUTHORS   Li,H., Gomes,P.J. and Chen,J.D.
  TITLE     RAC3, a steroid/nuclear receptor-associated coactivator that is
            related to SRC-1 and TIF2
  JOURNAL   Proc Natl Acad Sci U S A 94 (16), 8479-8484 (1997)
   PUBMED   9238002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On or before Nov 5, 2019 this sequence version replaced
            XM_006235634.2, XM_006224744.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643287.9864.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-123               JAXUCZ010000003.1  175157824-175157946
            124-203             JAXUCZ010000003.1  175200018-175200097
            204-308             JAXUCZ010000003.1  175212789-175212893
            309-481             JAXUCZ010000003.1  175213075-175213247
            482-582             JAXUCZ010000003.1  175213712-175213812
            583-757             JAXUCZ010000003.1  175215421-175215595
            758-943             JAXUCZ010000003.1  175216655-175216840
            944-1042            JAXUCZ010000003.1  175216954-175217052
            1043-1183           JAXUCZ010000003.1  175217750-175217890
            1184-1331           JAXUCZ010000003.1  175218135-175218282
            1332-1708           JAXUCZ010000003.1  175219672-175220048
            1709-2577           JAXUCZ010000003.1  175220168-175221036
            2578-2707           JAXUCZ010000003.1  175222275-175222404
            2708-2902           JAXUCZ010000003.1  175224044-175224238
            2903-3196           JAXUCZ010000003.1  175224554-175224847
            3197-3323           JAXUCZ010000003.1  175225064-175225190
            3324-3495           JAXUCZ010000003.1  175226133-175226304
            3496-3789           JAXUCZ010000003.1  175232964-175233257
            3790-3876           JAXUCZ010000003.1  175235164-175235250
            3877-4096           JAXUCZ010000003.1  175235721-175235940
            4097-4271           JAXUCZ010000003.1  175236564-175236738
            4272-4413           JAXUCZ010000003.1  175237197-175237338
            4414-4663           JAXUCZ010000003.1  175237582-175237831
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q42"
     gene            1..4663
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /note="nuclear receptor coactivator 3"
                     /db_xref="GeneID:84584"
                     /db_xref="RGD:620109"
     exon            1..123
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            124..203
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            204..308
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             223..4425
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /EC_number="2.3.1.48"
                     /note="AIB-1; NCoA-3; amplified in breast cancer-1 protein
                     homolog; thyroid hormone receptor activator molecule 1"
                     /codon_start=1
                     /product="nuclear receptor coactivator 3"
                     /protein_id="NP_445906.1"
                     /db_xref="GeneID:84584"
                     /db_xref="RGD:620109"
                     /translation="MSGLGESSLDPLATESRKRKLPCDAPGQGLVYSGEKWRREQESK
                     YIEELAELISANLSDIDNFNVKPDKCAILKETVRQIRQIKEQGKTISSDDDVQKADVS
                     STGQGVIDKDSLGPLLLQALDGFLFVVNRDGNIVFVSENVTQYLQYKQEDLVNTSVYS
                     ILHEQDRKDFLKHVPKSTVNGVSWTNENQRQKSHTFNCRMLMKTHDILEDTNASPETR
                     QRYETMQCFALSQPRAMLEEGEDLQCCMICVARRVTTDRPFPSSPESFITRHDLSGKV
                     VNIDTNSLRSSMRPGFEDTIRRCIQRFFSLNDGQSWSQKRHYQEAYIHGHAETPVYRF
                     SLADGTIVSAQTKSKLFRNPVTNDRHGFVSTHFLQREQNGCRPNPNPVGQGIRPPAAG
                     CGMSLSPSQSVQMLGSRTYGVADPSNTGQMAGARYGASSSVASLTPGQSLQSPSSYQS
                     NSYGLNMSSPPHGSPGLGPNQQNIMISPRNRGSPKMASHQFSPAAGVHSPMGSSGNTG
                     SHSFSSSSLSALQAISEGVGTSLLSTLSSPGPKLDNSPNMNINQPSKASSQDSKSPLG
                     LYCEQNPVESSVCPSNSRDHPSDKENKENSGEASETPRGPLESKGHKKLLQLLTCSSD
                     DRGHSSLTNSPLDSNCKDSSISVTSPSGVSSSTSGAVSSTSNMHGSLLQEKHRILHKL
                     LQNGNSPAEVAKITAEATGKDTSSTASGGEGSVRQEQLSPKKKENNALLRYLLDRDDP
                     SDVLAKELQPQADGGDSKLSQCSCSTNPSSGQEKDPKIKTEASEEVSGDLDNLDAILG
                     DLTSSDFYNSPTNGSHPGAKQQMFAGPSSLGLRSPQPVQSVRPPYNRALSLDSPVSVG
                     SVPPVKNVSAFPVLPKQPILAGNPRMMDSQENYGANMGGPNRNVPVNPTSSSGDWGLA
                     NSRASRMEPLASSPLGRAGGDYSAALPRPALGSSGPTLPLRSNRLPGARPTLQPQPQP
                     QQQQQQQQQQQQQQQMLQMRAGEVPMGMGVSPYSPAVPSNQPGSWPEGMLSMEQGPHG
                     AQNRPLLRNSLDELLGPPSNPEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPEL
                     VSQGQALESKQDVFQGQEAAVMMDQKAALYGQTYPAQGPPLQGGFHLQGQSPSLNSMM
                     SQISQQGSFPLQGLHPRASMVRPRTNTPKQLRMQLQQRLQGQQFLNQSRQALEMKMES
                     PTGAAVMRPMLQSQQAFFNAQMAAQQKRELMNHHLQQQRMAMMMSQPQPQAFSPPPNV
                     TASPSMDGVLAGSAMPQAPPQQFPYATNYGMGQPPEPAFGRGSSPPSAMMSSRMGPSQ
                     NAMVQHPQTAPMYQSSEMKGWPSGNLARNGSFPQQQFAPQANPAAYNMVHMNSSGSHL
                     GQMTMTPMPMSGMPMGPDQKYC"
     exon            309..481
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            482..582
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            583..757
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            758..943
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1042
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1183
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1331
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1708
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1709..2577
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2578..2707
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2708..2902
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2903..3196
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3197..3323
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3324..3495
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3496..3789
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3790..3876
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3877..4096
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4097..4271
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4272..4413
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
     exon            4414..4663
                     /gene="Ncoa3"
                     /gene_synonym="Aib1; Tram-1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagcggcggc tgcctcagct cctcagagcg ccggcggcgg cctcggcgga gtcggtgtcg
       61 gcggccgggg ctgagctgtg agtttccgat cgagaacagc gttggggaag atggcggcga
      121 gcggatcaaa agactcgctg aacagtggac cccgagatcg gtgaaactaa gctcttacct
      181 gacctcactg accaggccga cagctgctga gctgtggtca ggatgagtgg actgggtgaa
      241 agttccttgg atccactggc cactgagtct cggaaacgca aattgccctg tgacgctcca
      301 ggacaaggtc ttgtctacag tggtgaaaag tggagacggg agcaggagag caagtacata
      361 gaggaactgg ccgagctgat atctgcaaat cttagtgaca ttgacaactt caacgtcaaa
      421 ccagacaaat gtgccatttt aaaggagaca gtgagacaga tacgccaaat aaaagaacaa
      481 gggaaaacta tttccagtga cgatgatgtt cagaaagctg atgtgtcttc tacagggcag
      541 ggagtcattg ataaagactc tttaggaccg cttttactac aggcactgga tggctttctg
      601 tttgtggtga accgagatgg aaacattgta tttgtgtcag aaaatgtcac acagtatctg
      661 cagtacaagc aggaggacct ggttaacaca agtgtctaca gcatcttaca tgagcaggac
      721 cggaaggact ttcttaaaca cgtaccaaaa tccacagtta atggagtttc ttggacgaat
      781 gagaaccaga gacaaaaaag ccatacattt aattgtcgta tgctgatgaa aacacacgat
      841 atcttggaag acacgaatgc cagtcctgaa actcgccaga gatatgaaac aatgcagtgc
      901 ttcgccctgt ctcagcctcg cgctatgctg gaagaagggg aagacttgca gtgctgtatg
      961 atctgtgtgg ctcgccgtgt gactacagac cggccgttcc catccagtcc tgagagcttt
     1021 ataaccaggc acgacctttc agggaaggtt gtcaatatag atacaaactc acttagatct
     1081 tccatgaggc ctggcttcga agacacaatc cgaaggtgta tccagaggtt cttcagtctg
     1141 aatgacggac agtcatggtc ccagaagcgt cactatcagg aagcttacat tcatggccat
     1201 gcagagaccc cagtgtatcg tttctcattg gctgatggaa ctattgtgag tgctcagaca
     1261 aagagcaaac tcttccgcaa tcctgtaacg aatgatcgtc atggcttcgt ctcgacccac
     1321 ttccttcaga gagaacagaa tggatgtaga ccaaacccga atcctgtagg acaaggcatc
     1381 cgacctcctg cagcagggtg tggcatgagc ctgtctccaa gtcagagtgt gcagatgctg
     1441 ggcagccgga cctacggcgt ggccgacccc agcaacacag ggcagatggc tggagctagg
     1501 tatggggctt ctagtagtgt agcctcgctg accccaggac aaagcctgca gtcgccatct
     1561 tcctaccaga gcaacagcta tgggctcaac atgagcagtc ccccacacgg cagtcctggt
     1621 cttggcccca accagcagaa catcatgatt tctcctcgta atcgtgggag cccaaagatg
     1681 gcctcacacc agttctctcc tgctgcaggc gtgcactcgc ccatgggctc ttctggcaac
     1741 acagggagcc acagcttttc tagcagctcc ctcagtgcct tgcaagccat cagcgaaggt
     1801 gtgggaacct ctcttttatc tactctgtct tcaccaggcc ccaaattgga taactctccc
     1861 aatatgaata taaaccagcc aagtaaagcg agcagtcagg attctaagag ccctctaggc
     1921 ttatactgtg aacagaaccc agtggagagt tcggtgtgtc catcaaacag cagggatcac
     1981 cccagtgaca aagaaaacaa ggagaacagt ggggaggcgt cagagactcc caggggaccc
     2041 ctggaaagca aaggccacaa gaaactgctg cagttactca catgctcctc ggatgaccga
     2101 ggccattcct ccttgaccaa ctctcccctg gattcaaatt gcaaagactc ttccattagt
     2161 gtcaccagcc cctctggagt gtcgtcctcg acatcaggag cagtgtcttc cacctccaat
     2221 atgcatgggt ctctgttgca agagaaacac cggattttgc acaagttgct gcaaaatggc
     2281 aactcaccag cggaggttgc caagatcact gcagaagcca ctgggaaaga cactagcagt
     2341 actgcttccg gtggagaagg gagtgtcagg caggagcagc tgagtcctaa gaagaaggag
     2401 aataatgccc ttcttagata cctgctggac agggacgacc ccagtgatgt gcttgccaaa
     2461 gagctgcagc cccaagcgga cggaggggac agtaaactga gtcagtgcag ctgctccacc
     2521 aatcccagct ccggccaaga gaaagacccg aaaattaaga cagaggcaag tgaggaggta
     2581 tcgggagacc tggataacct agatgctatt cttggagatt tgactagttc tgacttctac
     2641 aacagtccta caaatggcag tcacccaggg gccaaacagc agatgtttgc aggaccaagt
     2701 tctctgggtt tgcgaagtcc acagcctgtg cagtctgtgc gccctccata taaccgagcg
     2761 ctgtctctag acagccccgt gtctgttggc tcagttccgc cagtgaagaa tgtcagtgct
     2821 ttccctgtgt taccaaaaca gcccatactg gctgggaatc caagaatgat ggatagtcag
     2881 gagaattacg gtgccaatat gggtggacca aacagaaacg ttcctgtgaa tccgacgtcc
     2941 tcctcaggag actggggttt agcgaactca agggccagca gaatggagcc tctggcttca
     3001 agtcccctgg gaagagctgg aggagactac agcgcagctt taccaagacc cgccttgggg
     3061 agctccgggc ctaccttgcc acttcgttct aatagactgc caggcgcaag accaacgttg
     3121 cagcctcagc cgcagccgca gcagcaacag cagcagcaac agcagcaaca gcagcagcag
     3181 cagatgcttc aaatgagagc tggtgaggtt cccatgggaa tgggcgtcag tccctatagc
     3241 ccagcagtgc catctaacca acccggatcg tggccagagg gcatgctctc gatggaacaa
     3301 ggtcctcatg gggctcaaaa taggcctctt cttagaaatt ccctggatga gctgctcggg
     3361 ccgccttcta acccagaagg ccagagtgat gagagagctc tgctggacca gctgcacacg
     3421 ctcctgagca acacggacgc cacaggcctg gaggagatcg acagggccct gggaattccc
     3481 gagcttgtga gtcagggaca agctttggag tccaaacagg atgttttcca aggccaagaa
     3541 gcagcagtaa tgatggatca gaaggctgca ctgtatggac aaacataccc agctcagggt
     3601 cctccactgc aaggaggctt tcaccttcag gggcagtccc cgtctctgaa ctctatgatg
     3661 agtcagatta gccagcaagg cagttttcct ctccaaggcc tgcatcctag agccagcatg
     3721 gtgagaccaa ggacaaacac cccgaagcag ctgagaatgc agcttcagca gcggctgcag
     3781 ggccagcagt ttttaaatca gagccggcag gcactggaga tgaagatgga aagccccact
     3841 ggtgctgctg tgatgaggcc catgctgcag tcccagcagg ctttctttaa tgcccaaatg
     3901 gcagcccagc agaaacgaga gctgatgaac catcacctgc agcagcagag gatggcgatg
     3961 atgatgtcac agccacagcc tcaggccttc agcccacctc ccaacgtcac cgcttccccc
     4021 agcatggacg gggtcttggc aggctcagca atgccacaag cccctccaca acagtttccg
     4081 tatgcgacaa attatggaat gggacaaccg ccagagccag cctttggtag gggctctagt
     4141 cctcccagtg caatgatgtc atcaagaatg gggccttccc agaatgccat ggtgcagcat
     4201 ccccagactg cacccatgta tcagtcctca gagatgaaag ggtggccatc agggaacttg
     4261 gccaggaatg gctcttttcc ccaacagcag tttgctcccc aggcgaaccc tgcagcatac
     4321 aacatggtgc acatgaacag cagtggtagt cacttgggac agatgaccat gactcccatg
     4381 cccatgtctg gcatgccgat gggtcctgat cagaaatact gctgacatgt ccctggtggg
     4441 accgagaagg aaatcactgt acagatgaca ctgcacagga ccattgggac ataaggagtc
     4501 attgtctagg catccagctt ggaagcaagg ccagcgtgac caccaccggg tgttcccaag
     4561 tgctgtcatc tgagcagaac tgggtctcaa gctgaagcgc actgtctacc tgatgccctg
     4621 cctctgtgtg gcatggtgtt ctgcctcatg aggatgtgat ctg
//
