LOCUS       NM_031822               8413 bp    mRNA    linear   ROD 18-FEB-2024
DEFINITION  Rattus norvegicus nuclear receptor coactivator 2 (Ncoa2), mRNA.
ACCESSION   NM_031822
VERSION     NM_031822.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Lin,Z., Yang,F., Lu,D., Sun,W., Zhu,G. and Lan,B.
  TITLE     Knockdown of NCOA2 Inhibits the Growth and Progression of Gastric
            Cancer by Affecting the Wnt Signaling Pathway-Related Protein
            Expression
  JOURNAL   Technol Cancer Res Treat 19, 1533033820928072 (2020)
   PUBMED   32489143
REFERENCE   2  (bases 1 to 8413)
  AUTHORS   Xu,W., Huang,J., Li,L., Zhang,X., Wang,Y., Tong,G. and Sun,Y.
  TITLE     Alterations of estradiol-induced histone H3 acetylation in the
            preoptic area and anteroventral periventricular nucleus of
            middle-aged female rats
  JOURNAL   Biochem Biophys Res Commun 516 (3), 894-899 (2019)
   PUBMED   31272713
  REMARK    GeneRIF: The data suggest that alterations of histone H3
            acetylation in the preoptic area and anteroventral periventricular
            nucleus and the inability of Ncoa2 and Crebbp to respond to
            estradiol in the middle-aged anterior hypothalamus partially
            contribute to the decline of GnRH neuron activation and estradiol
            target gene expression changes that occur in females along with
            reproductive aging.
REFERENCE   3  (bases 1 to 8413)
  AUTHORS   Hu,M., Zhang,Y., Feng,J., Xu,X., Zhang,J., Zhao,W., Guo,X., Li,J.,
            Vestin,E., Cui,P., Li,X., Wu,X.K., Brannstrom,M., Shao,L.R. and
            Billig,H.
  TITLE     Uterine progesterone signaling is a target for metformin therapy in
            PCOS-like rats
  JOURNAL   J Endocrinol 237 (2), 123-137 (2018)
   PUBMED   29535146
REFERENCE   4  (bases 1 to 8413)
  AUTHORS   Suresh,S., Durakoglugil,D., Zhou,X., Zhu,B., Comerford,S.A.,
            Xing,C., Xie,X.J., York,B. and O'Donnell,K.A.
  TITLE     SRC-2-mediated coactivation of anti-tumorigenic target genes
            suppresses MYC-induced liver cancer
  JOURNAL   PLoS Genet 13 (3), e1006650 (2017)
   PUBMED   28273073
  REMARK    Erratum:[PLoS Genet. 2018 Apr 11;14 (4):e1007344. PMID: 29641518]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8413)
  AUTHORS   Fleet,T., Stashi,E., Zhu,B., Rajapakshe,K., Marcelo,K.L.,
            Kettner,N.M., Gorman,B.K., Coarfa,C., Fu,L., O'Malley,B.W. and
            York,B.
  TITLE     Genetic and Environmental Models of Circadian Disruption Link SRC-2
            Function to Hepatic Pathology
  JOURNAL   J Biol Rhythms 31 (5), 443-460 (2016)
   PUBMED   27432117
REFERENCE   6  (bases 1 to 8413)
  AUTHORS   Chung,H.J., Xia,J., Scannevin,R.H., Zhang,X. and Huganir,R.L.
  TITLE     Phosphorylation of the AMPA receptor subunit GluR2 differentially
            regulates its interaction with PDZ domain-containing proteins
  JOURNAL   J Neurosci 20 (19), 7258-7267 (2000)
   PUBMED   11007883
REFERENCE   7  (bases 1 to 8413)
  AUTHORS   Martinez de Arrieta,C., Koibuchi,N. and Chin,W.W.
  TITLE     Coactivator and corepressor gene expression in rat cerebellum
            during postnatal development and the effect of altered thyroid
            status
  JOURNAL   Endocrinology 141 (5), 1693-1698 (2000)
   PUBMED   10803578
REFERENCE   8  (bases 1 to 8413)
  AUTHORS   Atkins,G.B., Hu,X., Guenther,M.G., Rachez,C., Freedman,L.P. and
            Lazar,M.A.
  TITLE     Coactivators for the orphan nuclear receptor RORalpha
  JOURNAL   Mol Endocrinol 13 (9), 1550-1557 (1999)
   PUBMED   10478845
REFERENCE   9  (bases 1 to 8413)
  AUTHORS   Leers,J., Treuter,E. and Gustafsson,J.A.
  TITLE     Mechanistic principles in NR box-dependent interaction between
            nuclear hormone receptors and the coactivator TIF2
  JOURNAL   Mol Cell Biol 18 (10), 6001-6013 (1998)
   PUBMED   9742117
REFERENCE   10 (bases 1 to 8413)
  AUTHORS   Hong,H., Kohli,K., Trivedi,A., Johnson,D.L. and Stallcup,M.R.
  TITLE     GRIP1, a novel mouse protein that serves as a transcriptional
            coactivator in yeast for the hormone binding domains of steroid
            receptors
  JOURNAL   Proc Natl Acad Sci U S A 93 (10), 4948-4952 (1996)
   PUBMED   8643509
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000157.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by NM_031822.3.
            
            On Sep 8, 2022 this sequence version replaced NM_031822.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AF136943.1 [ECO:0000331]
            RNAseq introns       :: partial sample support SAMD00132261,
                                    SAMD00132262 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-131               JACYVU010000157.1  4990928-4991058
            132-187             JACYVU010000157.1  5079461-5079516
            188-273             JACYVU010000157.1  5143259-5143344
            274-446             JACYVU010000157.1  5146509-5146681
            447-550             JACYVU010000157.1  5177301-5177404
            551-728             JACYVU010000157.1  5180782-5180959
            729-917             JACYVU010000157.1  5183169-5183357
            918-1019            JACYVU010000157.1  5186163-5186264
            1020-1163           JACYVU010000157.1  5186809-5186952
            1164-1311           JACYVU010000157.1  5189798-5189945
            1312-2581           JACYVU010000157.1  5191731-5193000
            2582-2792           JACYVU010000157.1  5199681-5199891
            2793-2999           JACYVU010000157.1  5201480-5201686
            3000-3215           JACYVU010000157.1  5204220-5204435
            3216-3345           JACYVU010000157.1  5206462-5206591
            3346-3511           JACYVU010000157.1  5208581-5208746
            3512-3790           JACYVU010000157.1  5211992-5212270
            3791-3868           JACYVU010000157.1  5212627-5212704
            3869-4103           JACYVU010000157.1  5214239-5214473
            4104-4281           JACYVU010000157.1  5215955-5216132
            4282-4480           JACYVU010000157.1  5216618-5216816
            4481-4573           JACYVU010000157.1  5218283-5218375
            4574-8413           JACYVU010000157.1  5221140-5224979
FEATURES             Location/Qualifiers
     source          1..8413
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q11"
     gene            1..8413
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /note="nuclear receptor coactivator 2"
                     /db_xref="GeneID:83724"
                     /db_xref="RGD:620108"
     exon            1..131
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            132..187
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     CDS             188..4585
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /function="nuclear hormone receptor coactivator"
                     /note="NCoA-2; transcriptional intermediary factor 2;
                     glucocorticoid receptor interacting protein 1"
                     /codon_start=1
                     /product="nuclear receptor coactivator 2"
                     /protein_id="NP_114010.2"
                     /db_xref="GeneID:83724"
                     /db_xref="RGD:620108"
                     /translation="MSGMGENTSDPSRAETRKRKECPDQLGPSPKRSTEKRNREQENK
                     YIEELAELIFANFNDIDNFNFKPDKCAILKETVKQIRQIKEQEKAAAANIDEVQKSDV
                     SSTGQGVIDKDALGPMMLEALDGFFFVVNLEGNVVFVSENVTQYLRYNQEELMNKSVY
                     SILHVGDHTEFVKNLLPKSMVNGGSWTGEPPRRNSHTFNCRMLVKPLPDSEEEGHDNQ
                     EAHQKYETMQCFAVSQPKSIKEEGEDLQSCLICVARRVPMKERPALPSSESFTTRQDL
                     QGKITSLDTSTMRAAMKPGWEDLVRRCIQKFHTQHEGESLSYAKRHHHEVLRQGLAFS
                     QIYRFSLSDGTLVAAQTKSKLIRSQTTNEPQLVISLHMLHREQNVCVMNPDLTGQAMG
                     KPLSPMSSSSPARQAMCSGNPGQDVALGSNMNFPMNGPREQMSMPMGRFGGSGGMNHV
                     SGMQATTPQGSNYALKMNSPSQSSPGLNPGQPSSVLSPRHRMSPGVAGSPRVPPSQFS
                     PAGSLHSPAGVCSSTGNSHSYTNSSLNALQALSEGHGVSLGPSLASPDLKMGNSQNSP
                     VNMNPPPLSKMGSLDSKDCFGLYGEPSEGTTGQAEASCHPEEQKRPNDSSMPQAASGD
                     RAEGHSRLHESKGQTKLLQLLTTKSDQMEPSPLPSSLSDTNKDSTGSLPGPGSTHGTS
                     LKEKHKILHRLLQDSSSPVDLAKLTAEATGKELNQESSGTAPGSEVTVKQEPASPKKK
                     ENALLRYLLDKDDTKDIGLPEITPKLERLDSKTDPASNTKLIAMKTVKEEVSFEPSDQ
                     PGSELDNLEEILDDLQNSQLPQLFPDTRPGAPTGSVDKQAIINDLMQLTADSSPVTPV
                     GAQKAALRMSQSTFNNPRPGQLGRLLPNQNLPLDITLQSPTGAGPFPPIRNSSPYSVI
                     PQPGMMGNQGMLGSQGNLGNNSTGMIGSSTSRSSMPSGEWAPQSPAVRVTCAATTGAM
                     NRPIQGGMIRNPTASIPMRANSQPGQRQMLQPQVMNIGPSELEMNMGGPQYNQQQAPP
                     NQTAPWPESILPIDQASFGSQNRHPFGSSPDDLLCPHPAAESPSDEGALLDQLYLALR
                     NFDGLEEIDRALGIPELVSQSQAVDPEQFSSQESSMMLEQKPPVFPQQYASQTQMAQG
                     SYNPMQDPNFHTMGQRPNYTTLRMQPRPGLRPTGIVQNQPNQLRLQLQHRLQAQQNRQ
                     PLMNQISGVSNVNLTLRPGVPTQAPINAQMLAQRQREILNQHLRQRQMHQQQQVQQRT
                     LMMRGQGLNMTPSMVAPTGLPAAMSNPRIPQANAQQFPFPPNYGISQQPDPGFTGATT
                     PQSPLMSPRMAHTQSPMMQQSQANPAYQPASDMNGWAQGSMGGNSMFSQQSPPHFGQQ
                     ANTSMYNNNMNINVSMATNTAGLSNMNQMTGQMSMTSVTSVPTSGLSSMGPEQVNDPA
                     LRGSSLFTTNQLPGMDMIKQEGDGSRKYC"
     exon            188..273
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            274..446
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            447..550
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            551..728
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            729..917
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1019
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1163
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1311
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..2581
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            2582..2792
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            2793..2999
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            3000..3215
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            3216..3345
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            3346..3511
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            3512..3790
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            3791..3868
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            3869..4103
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            4104..4281
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            4282..4480
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            4481..4573
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
     exon            4574..8413
                     /gene="Ncoa2"
                     /gene_synonym="GRIP-1; Grip1; Tif2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctccattgaa ataaacaacc aggcagcagt tattaacacg ggaacatggc ggccgcagcc
       61 tgggctcccg cggcggcggc ggaggtcagc gccgacggca gcccgcacct gacggcgtga
      121 cggccacatt gactcttcgt gcaccgggcc cagtctatca gatcttctgc actgtgtatc
      181 tgcacagatg agtgggatgg gagaaaatac ctctgacccg tccagggcag agaccagaaa
      241 acgcaaggaa tgtccggacc agctcggacc cagccccaaa aggagcacgg agaaacgtaa
      301 ccgcgagcag gaaaataagt acatagagga actcgcagag ctgatctttg caaactttaa
      361 tgatatcgac aacttcaact tcaaacctga caaatgtgcc atcttaaaag aaactgtgaa
      421 acagatccgc cagatcaaag agcaagagaa agcagccgct gccaacatcg atgaagtgca
      481 gaagtcagat gtgtcgtcca cggggcaggg cgtcatcgac aaggacgcac tggggcctat
      541 gatgcttgag gcccttgatg ggttcttctt cgtcgtgaac ctagaaggca atgtggtgtt
      601 cgtgtccgag aacgtgacgc agtatctgcg gtataaccag gaagaactga tgaacaagag
      661 cgtttacagc atcctgcatg tcggggacca cacggaattt gtcaagaacc tgctgccaaa
      721 gtccatggtg aatgggggat cctggaccgg agaacctccc aggcggaaca gccatacctt
      781 caactgtcgg atgctggtga agcctttgcc agattcagaa gaggaaggcc atgataacca
      841 ggaagcacat cagaaatacg agacgatgca gtgctttgct gtgtctcagc caaagtctat
      901 caaagaggag ggcgaagatt tgcagtcctg cttgatttgt gtggcacgaa gagtccccat
      961 gaaggaaagg ccagcccttc cctcatcaga gagctttacc acccgccagg acctccaagg
     1021 caagatcact tctctggaca ctagcaccat gagagctgcc atgaaaccgg gctgggagga
     1081 tctggtaaga agatgtattc agaagttcca cacacagcat gaaggggagt ctctctcata
     1141 cgccaagagg catcaccatg aagttctgag acaaggcttg gcgttcagtc agatctatcg
     1201 tttttccttg tccgacggca ctctcgttgc tgcacaaacg aagagcaaac tcatccgttc
     1261 ccagactacg aatgagcctc agcttgtgat atcgctacac atgcttcaca gagagcagaa
     1321 tgtgtgtgta atgaatccgg atctgactgg acaagcaatg gggaagccat tgagtccaat
     1381 gagctctagc agccccgccc gtcaggccat gtgcagtggg aacccaggtc aggatgtggc
     1441 cctcggtagc aatatgaact ttcccatgaa tggcccaagg gaacagatga gcatgcctat
     1501 gggcaggttt ggtggttctg ggggcatgaa ccatgtatca ggcatgcaag caaccactcc
     1561 tcagggtagt aactatgcac tcaaaatgaa cagtccctca caaagcagcc ccggcctgaa
     1621 cccagggcaa cccagctctg tgctctcccc acggcatcgc atgagccccg gcgtggctgg
     1681 cagtcctcgc gtcccaccca gtcagttttc ccctgcagga agcttgcact ccccagcggg
     1741 agtttgcagc agcacaggaa atagccatag ttacaccaac agttccctca atgcactgca
     1801 agccctcagc gagggccacg gggtctcgtt agggccatcg ttggcttcac cggacctaaa
     1861 aatgggcaat tcgcaaaact ccccagttaa catgaatcct cccccactca gcaagatggg
     1921 aagcttggac tccaaagact gttttggact ttatggggag ccatcagaag gtacaactgg
     1981 acaagcagag gccagctgcc atcctgaaga gcagaagagg cccaatgatt ccagcatgcc
     2041 ccaggcggcc agcggggaca gggctgaggg acacagccgg ctgcatgaga gcaaagggca
     2101 aaccaaactc ctacagctgc tcaccaccaa gtccgaccag atggagcctt cacccttgcc
     2161 cagctctttg tcggacacaa acaaggactc cacaggcagc ttgccagggc ctgggtccac
     2221 gcatggcacc tcgctcaagg agaagcataa gattttgcac agactcttac aggacagcag
     2281 ctcccctgtg gacttggcca agctgacagc agaagccaca ggcaaagagc tgaatcagga
     2341 gtccagtggc acggctcctg ggtcagaagt gactgtcaaa caggagccag cgagccccaa
     2401 gaagaaagag aacgcactac tgcgctattt gctcgacaaa gatgatacta aagatattgg
     2461 tttaccggaa ataaccccca aactcgagcg gctggacagt aagacagatc ctgccagtaa
     2521 cacaaagcta atcgccatga aaactgtgaa ggaggaggtg agctttgagc ccagtgacca
     2581 gcctggcagc gagctggaca acttggaaga gattttggat gatttgcaga atagtcagtt
     2641 accacagctt ttcccagaca caaggccagg agctcctact gggtcagttg acaagcaagc
     2701 catcatcaat gacctcatgc aactcacggc tgacagcagt cctgtcacac ctgtcggagc
     2761 ccagaaagca gcactgcgaa tgtcacagag cacttttaat aacccacgac cagggcaact
     2821 gggcaggtta ttgccaaacc agaatttacc acttgacatc acattgcaaa gcccaactgg
     2881 tgctggacct ttcccaccaa tcagaaacag tagtccctac tcagtgatac ctcagccagg
     2941 aatgatgggt aaccaaggga tgctaggaag ccaaggaaac ttagggaaca atagcacagg
     3001 aatgattggc agtagcactt cccggtccag catgccttct ggggaatggg caccacagag
     3061 tccagctgtg agagtcactt gtgctgccac cactggtgcc atgaaccggc caatccaagg
     3121 gggcatgatt cggaacccaa cagcaagcat ccccatgcga gccaacagcc agcctggcca
     3181 aagacagatg ctgcagcctc aggtcatgaa catagggcct tctgagttgg agatgaacat
     3241 gggaggccct cagtataatc aacagcaggc ccctccaaac caaactgccc cctggcccga
     3301 gagcatcctg cctatagacc aggcatcttt cggcagccag aacaggcatc cctttggcag
     3361 ctcccccgac gacctgctct gtccacatcc tgcagccgag tccccaagcg acgagggcgc
     3421 tcttctagac cagctctatc tggccttgcg gaacttcgat ggccttgaag agattgatag
     3481 agctctgggg ataccagaac tggtcagcca gagccaagct gtggatccgg agcagttctc
     3541 gagtcaggag tccagcatga tgctggagca gaagccccct gttttcccac agcagtacgc
     3601 atctcagaca caaatggccc aggggagcta taatcccatg caagatccaa acttccacac
     3661 catgggacag cggccaaatt ataccacact ccgaatgcag ccccggccgg gcctcaggcc
     3721 cacaggcatt gtgcagaacc agccaaacca actgagactt cagctccaac atcgcctcca
     3781 agcacagcag aaccgccagc cgctaatgaa ccagatcagc ggcgtttcca atgtgaactt
     3841 gactctgagg cctggcgtgc caactcaggc tcctattaat gcacagatgc tggcccagag
     3901 gcagagggaa atccttaacc agcatctccg gcagagacag atgcatcagc agcagcaggt
     3961 gcagcagcga actttgatga tgagaggaca agggttgaat atgaccccaa gcatggtggc
     4021 tcccactggt ctaccagcag ccatgagcaa cccccggatc ccgcaagcca atgcccagca
     4081 gtttccattt cctccgaact acggaataag tcagcaacct gatcctggct ttactggagc
     4141 tacaactccc cagagtcctc taatgtctcc ccggatggca catacgcaga gtcccatgat
     4201 gcagcagtct caggccaacc cagcctacca gcccgcctca gacatgaacg gatgggcaca
     4261 gggaagcatg ggcggaaaca gcatgttctc acaacagtcc ccaccacact ttgggcaaca
     4321 agcgaacacc agcatgtaca ataacaacat gaacatcaac gtgtccatgg caaccaacac
     4381 ggctggcttg agcaacatga accagatgac aggccagatg agcatgacct cagtgacctc
     4441 cgtgcctaca tcagggctgt cctccatggg tcctgagcag gtcaatgacc ctgctctgag
     4501 gggaagcagc cttttcacca caaaccaact gcctggaatg gacatgatca agcaggaggg
     4561 agatgggtct cggaaatact gctgaccctg gggaagctgt ctgcgtcttt cttctgggct
     4621 tacaaaacct ttagcagtct ggagaactgc gtccttgtgt tgacccacct gacccaccac
     4681 cagttctcca ggacacatca gcagacagtt gggccctggc tcgcagcagc atagagcctg
     4741 ctggcttggc gccaccggaa gagctgcctc tcctgactgg cagcctgcag ctcacctcca
     4801 gaccaacccg cagtctgttc actgcattca ccgtagtgca acttagatct cctgcagtaa
     4861 acgtccacag gccgactcca tccccattca gattgaatgt atttaaatgt gtgtatttgg
     4921 agaaccacgc tcttgttctg ttcctgttca gctccagaca ctggtttctt gctttgtttt
     4981 ccgtggctat tcagtgcagt gtaaaagatt gtgatcttac ctagagaaag aaaggaattt
     5041 tgtaccacag tccagcacaa ggtgttctag gcttacagtc tgcatttggt cgggatggag
     5101 gcaggggaca ctgtggacag cactgtgtgc tgacacacct agtcagggac agctgacaca
     5161 ggcagagttc catctccagc agccaagact gagagaagcc gctcacatta acaagtatct
     5221 gttcccattt ttctttgtta aaaacaaact agtttccccg aaatcatgaa tcaaaaggaa
     5281 atatttgttt ctttttcttt tttttttttt ttcagagctg gggaccgaac ccagggcctt
     5341 gcgcttgcta ggcaagcgct ctaccactga gctaaatccc caacccggaa atatttattt
     5401 ctaaattgtt tttagatcca tcagatagtt tgattggcct ctcctccctt cccctctccg
     5461 ccttctctcg tccctccctc tttcattcct ttaaaacaaa aacatcaaca acagcatcca
     5521 ccatagagac cagggaagcc ctttgcttct ctcagtgttg tgccagccac ttgcagcagc
     5581 tccccagtga ccgtgcattt cctgtcaggg agaggaacaa agtagacatg gaatgccagt
     5641 caggctgtga cacctgctta ggaagcctgg gctgccccat cagctcaggc ctccaaaacg
     5701 agagaacata ctgatgaaat cggcagcagg cgtcacgaca gatctaacct ctgccatgtg
     5761 gggttttttt gtttttaatt ttgttttcta gcagtgctga ctaagccgaa gttttgtaag
     5821 gtacataaaa atccagttta tatgtcaacg agcaataatt taagttctga acttaagtgt
     5881 tttaactgta taattttgtg aggtatacat attgtgggat tgactcaaac aatgaggtac
     5941 ttcagtatta aattagatat cttcatagca ttgtctccta aaggtgtttt gtaaaggcag
     6001 ccaatgcctt gattagacct gatttgtaga cttaagacct tttattttct aaaccttgag
     6061 attctgctca tgaatcatgt atctaatcta tataatatgc agccgctgta ggaaccaagt
     6121 cttgatttta tatgtttata ttctttctta ataaacctta gaaagactac attactaagc
     6181 agcccacttt gaaggttgct tttcttgccc agcgttgggg ataaccttta tcagttggtg
     6241 tcgtttgaga cagctgcagt acccagaaat gagaccttct caatcctccc caaccctgac
     6301 acacacacac tttcttacct gaatttcaaa gtgatatctt tttgatgtct aaaaacaaag
     6361 cattttataa tgtctcatta tccaagcttt ctagggacag agaagttttt ccttgaaatt
     6421 tgtttgttta ttcctggcag ggtgaaaaac ataaactacc ctatttatat gtatatctcc
     6481 ctatatttct acaggttgta gaaatataga cagagatatg acccaaagaa aatagtaaac
     6541 catctaaaag tgttgctccg tgctaaggaa ctttctggaa atttgcattt cgataccttt
     6601 ttccacattg agagaaatct cagtgcagag aggaacaaca cttcccagtt ggccgccagt
     6661 tcacgtgtct gctcgagact ccactttttc tccactgaac cattgggttg cacatctgtg
     6721 ctgcagagtg gcagacgtgg cccagaactc caggccccgc cccggggggt acactactga
     6781 gcaaaatgtg aggtaggcga taacttttag cttcacacag cacaagagaa aatggaattt
     6841 aaagcaggtt tggtttggtt tggtttggtt tggtttggtt tggtttggtt tggtttggtt
     6901 tggtttggtt tgtattaaat cccatctcac tctaacacca gccacactat cttacctttc
     6961 agcggtcatt cactctacca ctgttttcta atgttgaccc caaaccagac aagggttccg
     7021 acccagcagt cagagtagcc catgatcatg acaggaagag gggaaatcct ctcacaggtc
     7081 agagcagcca cactgggttc catccagtca ggaggatcaa gtcaggaagg agtcctctga
     7141 tcatgtcctc agaagctaat gccaacccag cctcttccat cagtattgct cgctctgcac
     7201 tgtacatgtg cacttggctt tgtttgacag agcatagaat cagtgttttc ttagatcact
     7261 caatgtgaag agcataatgt gaaaagtgca cacacctcat cttggttgta acatccattt
     7321 acgtgttcat ggaacatgat gagttttaaa agcaaagtga gctgtgttac tgcaaccgat
     7381 ttgttcaaag atgaaaacac ccaacacgcg cgcgcgcgcg cgcgcgcgca cgcgcgcaca
     7441 cacacacaca cacgcacaca tgtgtatctc ttacagtgta atgattgaaa gcaatggttt
     7501 tttgcagttt aggacgccat tttgaatctg tgtttaaaac ggagttcatc tgggtctgac
     7561 acatgaactg gaggcgcaga tccgcttgca gaacccaaag acacacaatc aattttgaaa
     7621 tgcaacttaa gccattaact gttggtgtga atttaaaatt ttctagtgtt tgtatggtag
     7681 tcttgctgcc gaagagcaaa gcattctctg cacaaaagaa aatactgtag tggcttcgat
     7741 tttaattaga actttctccc tggtgtttct ttatagacta aaacaaaatc ttttaagttt
     7801 cttactacag gtaaaagcta tgtgcaagaa cctcaaaatg ctaaaaccta gcataatgcc
     7861 ttagatctgt ttttaagtct tgtatattcc tacatagctg tccaatgtat tatatttgta
     7921 tagtgaattg tgtacaattt ttgaataagt acgtcaccgc caacccatat gttattgagc
     7981 agtgatgagg acccatctct tcaaacattt acctgtctct cttcctttgt catttgtggg
     8041 ttttatttgt acagttcctc atgaagttgc atctgagata tgtgtatatg aaaagattat
     8101 acaagggtaa aattaagcaa tatattaact atggttcttc aggagaaagc tcacagtgtt
     8161 tcaggttgtg atttattttc aaaacagagt tggctctgaa catcactttg ttcatctaca
     8221 tcgatgtttg tatttctagt gtgagcagtt tatgcaaagg tggatatatt cagagatttt
     8281 aaatacattt atgcaagtta ctattgcttc gctgatgctc tttgcttctt tgatgttaaa
     8341 taatgctatt gtaaataaag aatccgttgt tactgcatcg tttaataaat ttcaatgcct
     8401 tcttcgggtt tta
//
