LOCUS       NM_031618               4270 bp    mRNA    linear   ROD 26-APR-2024
DEFINITION  Rattus norvegicus NCK-associated protein 1 (Nckap1), mRNA.
ACCESSION   NM_031618 XM_001067975 XM_230038
VERSION     NM_031618.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Afzal,T.A., Luong,L.A., Chen,D., Zhang,C., Yang,F., Chen,Q., An,W.,
            Wilkes,E., Yashiro,K., Cutillas,P.R., Zhang,L. and Xiao,Q.
  TITLE     NCK Associated Protein 1 Modulated by miRNA-214 Determines Vascular
            Smooth Muscle Cell Migration, Proliferation, and Neointima
            Hyperplasia
  JOURNAL   J Am Heart Assoc 5 (12), e004629 (2016)
   PUBMED   27927633
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Chazeau,A., Mehidi,A., Nair,D., Gautier,J.J., Leduc,C., Chamma,I.,
            Kage,F., Kechkar,A., Thoumine,O., Rottner,K., Choquet,D.,
            Gautreau,A., Sibarita,J.B. and Giannone,G.
  TITLE     Nanoscale segregation of actin nucleation and elongation factors
            determines dendritic spine protrusion
  JOURNAL   EMBO J 33 (23), 2745-2764 (2014)
   PUBMED   25293574
REFERENCE   3  (bases 1 to 4270)
  AUTHORS   De Rubeis,S., Pasciuto,E., Li,K.W., Fernandez,E., Di Marino,D.,
            Buzzi,A., Ostroff,L.E., Klann,E., Zwartkruis,F.J., Komiyama,N.H.,
            Grant,S.G., Poujol,C., Choquet,D., Achsel,T., Posthuma,D.,
            Smit,A.B. and Bagni,C.
  TITLE     CYFIP1 coordinates mRNA translation and cytoskeleton remodeling to
            ensure proper dendritic spine formation
  JOURNAL   Neuron 79 (6), 1169-1182 (2013)
   PUBMED   24050404
REFERENCE   4  (bases 1 to 4270)
  AUTHORS   Kuo,J.C., Han,X., Hsiao,C.T., Yates,J.R. 3rd and Waterman,C.M.
  TITLE     Analysis of the myosin-II-responsive focal adhesion proteome
            reveals a role for beta-Pix in negative regulation of focal
            adhesion maturation
  JOURNAL   Nat Cell Biol 13 (4), 383-393 (2011)
   PUBMED   21423176
REFERENCE   5  (bases 1 to 4270)
  AUTHORS   Chen,Z., Borek,D., Padrick,S.B., Gomez,T.S., Metlagel,Z.,
            Ismail,A.M., Umetani,J., Billadeau,D.D., Otwinowski,Z. and
            Rosen,M.K.
  TITLE     Structure and control of the actin regulatory WAVE complex
  JOURNAL   Nature 468 (7323), 533-538 (2010)
   PUBMED   21107423
REFERENCE   6  (bases 1 to 4270)
  AUTHORS   Derivery,E., Fink,J., Martin,D., Houdusse,A., Piel,M.,
            Stradal,T.E., Louvard,D. and Gautreau,A.
  TITLE     Free Brick1 is a trimeric precursor in the assembly of a functional
            wave complex
  JOURNAL   PLoS One 3 (6), e2462 (2008)
   PUBMED   18560548
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4270)
  AUTHORS   Rakeman,A.S. and Anderson,K.V.
  TITLE     Axis specification and morphogenesis in the mouse embryo require
            Nap1, a regulator of WAVE-mediated actin branching
  JOURNAL   Development 133 (16), 3075-3083 (2006)
   PUBMED   16831833
REFERENCE   8  (bases 1 to 4270)
  AUTHORS   Steffen,A., Rottner,K., Ehinger,J., Innocenti,M., Scita,G.,
            Wehland,J. and Stradal,T.E.
  TITLE     Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia
            formation
  JOURNAL   EMBO J 23 (4), 749-759 (2004)
   PUBMED   14765121
REFERENCE   9  (bases 1 to 4270)
  AUTHORS   Kitamura,T., Kitamura,Y., Yonezawa,K., Totty,N.F., Gout,I.,
            Hara,K., Waterfield,M.D., Sakaue,M., Ogawa,W. and Kasuga,M.
  TITLE     Molecular cloning of p125Nap1, a protein that associates with an
            SH3 domain of Nck
  JOURNAL   Biochem Biophys Res Commun 219 (2), 509-514 (1996)
   PUBMED   8605018
REFERENCE   10 (bases 1 to 4270)
  AUTHORS   Baumgartner,S., Martin,D., Chiquet-Ehrismann,R., Sutton,J.,
            Desai,A., Huang,I., Kato,K. and Hromas,R.
  TITLE     The HEM proteins: a novel family of tissue-specific transmembrane
            proteins expressed from invertebrates through mammals with an
            essential function in oogenesis
  JOURNAL   J Mol Biol 251 (1), 41-49 (1995)
   PUBMED   7643388
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB586461.1, D84346.1, BC107935.1 and BC092610.1.
            
            On or before Oct 12, 2007 this sequence version replaced
            XM_230038.4, XM_001067975.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D84346.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-96                CB586461.1         1-96
            97-1904             D84346.1           1-1808
            1905-2505           BC107935.1         465-1065
            2506-4270           BC092610.1         459-2223
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q24"
     gene            1..4270
                     /gene="Nckap1"
                     /note="NCK-associated protein 1"
                     /db_xref="GeneID:58823"
                     /db_xref="RGD:61939"
     exon            1..204
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     CDS             97..3483
                     /gene="Nckap1"
                     /note="NAP 1; p125Nap1; membrane-associated protein HEM-2"
                     /codon_start=1
                     /product="nck-associated protein 1"
                     /protein_id="NP_113806.1"
                     /db_xref="GeneID:58823"
                     /db_xref="RGD:61939"
                     /translation="MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAK
                     PSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVME
                     FKDHVCDLLNTIDVCQVFFDITVNFDLTKNYLDLTVTYTTLMILLSRIEERKAIIGLY
                     NYAHEMTHGASDREYPRLGQMIVDYEHPLKKMMEEFVPHSKSLSDALISLQMVYPRRN
                     LSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGMLNTE
                     ATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSH
                     AGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNM
                     PKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLS
                     VCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLAD
                     HRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYS
                     IAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTL
                     SDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTAL
                     SELCFSINYVPNMAVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELL
                     TSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLR
                     QVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLM
                     WHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVIL
                     SFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEID
                     PALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIH
                     CLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLL
                     DMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA"
     exon            205..315
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            316..408
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            409..465
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            466..608
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            609..699
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            700..837
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            838..886
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1043
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1100
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1197
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1304
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1437
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1438..1519
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1578
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1579..1724
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1725..1857
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1858..1977
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..2117
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2118..2249
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2250..2468
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2469..2599
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2600..2697
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2791
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2792..2874
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2875..2955
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            2956..3049
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            3050..3166
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            3167..3276
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            3277..3366
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
     exon            3367..4250
                     /gene="Nckap1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctgaggcgg aggtcgcggg cgggcgggcg ggcgggcctg agggaagcgg cgagccgggg
       61 agtagcgcgc ccggcggcgg cggcggtggc cgcaccatgt cgcgctccgt gctgcagccc
      121 agtcagcaga agctggcgga gaagctcacc atcctcaacg accggggcgt cggcatgctc
      181 acgcgcctct acaacatcaa gaaggcatgt ggagatccca aggctaaacc atcctacctt
      241 attgacaaaa acctggaatc tgctgtgaaa ttcatagtca gaaaatttcc tgctgtagaa
      301 acacgcaaca acaatcaaca gcttgcacaa ctacagaaag aaaaatcaga gattctgaaa
      361 aatctggcat tatattactt tacatttgta gatgttatgg aatttaagga ccatgtctgt
      421 gacttgctga atactatcga cgtctgccaa gtcttctttg atattactgt gaactttgat
      481 ttaacaaaga actacttaga cttgactgtg acctacacaa cattaatgat actgctgtcc
      541 cggattgaag aaaggaaggc catcattgga ctgtacaatt atgcacatga aatgacccac
      601 ggagcaagtg accgagaata cccacgcctt ggtcagatga ttgtggacta tgaacaccct
      661 ttaaagaaga tgatggaaga attcgtaccc cacagcaagt ctctttcaga tgcgctaatt
      721 tctcttcaga tggtgtatcc tagacggaat ctttcagcag atcagtggag gaatgcccag
      781 ttgttgagtc tcatcagtgc acccagtaca atgcttaatc ctgcacagtc tgacaccatg
      841 ccttgtgaat atctctcttt ggatgcaatg gagaaatgga ttatctttgg ctttattctg
      901 tgccatggga tgctaaatac cgaggctaca gcactgaacc tttggaagct agctcttcag
      961 agtagctcct gcctctctct ctttcgggat gaagttttcc acattcacaa agctgcagaa
     1021 gatttatttg taaacattcg gggctataat aaacggatta acgacataag agaatgtaag
     1081 gaggcggctg tatcccacgc tggctcaatg cacagagaga gacgcaagtt tttacggtct
     1141 gcactgaaag aactggcaac tgtcctctcg gatcagcctg gcctgcttgg tcccaaggca
     1201 ctttttgttt tcatggcatt atcttttgcc cgtgatgaaa ttatctggct actccgtcat
     1261 gcagacaaca tgccaaagaa gagtgcagat gactttatag ataagcacat tgctgagctg
     1321 atcttttaca tggaggagct cagagcacat gtcaggaagt acgggcctgt catgcagagg
     1381 tactacgtgc agtacctctc cggcttcgat gctgttgtcc ttaacgagct tgtgcagaat
     1441 ctctctgttt gccctgaaga tgaatcaatc atcatgtcct cctttgttaa caccatgact
     1501 tccctaagtg tgaaacaagt tgaagatggg gaagtgtttg atttcagagg aatgagatta
     1561 gattggttta ggttacaggc atacactagt gtctccaaag cttcacttag ccttgcagat
     1621 caccgagaac tcggaaagat gatgaacaca ataatattcc atacaaagat ggtcgattcc
     1681 ttggtggaaa tgttggtgga aacatccgat ctctccatat tttgttttta tagccgtgct
     1741 tttgagaaga tgtttcagca gtgcttggag ttgccttctc agtcgcgata ctccatcgcc
     1801 tttcctctgc tctgcactca cttcatgagc tgcacacatg agctgtgtcc cgaagagcgc
     1861 catcatatag gggatcgtag cctttccctg tgtaatatgt tcctcgatga gatggccaaa
     1921 caagctcgga acctcatcac tgatatttgc acagaacagt gtactcttag tgaccagtta
     1981 ctgccgaagc attgtgccaa aaccatcagt caagcagtga ataagaagtc aaaaaaacag
     2041 actggcaaga aaggggaacc tgaaagggaa aagccgggtg tcgagagcat gaggaaaaac
     2101 aggctggtag tgaccaacct tgataagttg cacactgcac tttcggagtt atgcttctcc
     2161 ataaattatg ttccaaacat ggcagtgtgg gaacacacct tcacaccgcg ggagtacttg
     2221 acttctcatc tggaaatccg attcactaaa tcaattgttg gaatgaccat gtataatcag
     2281 gctacacagg agattgcaaa gccatcagaa cttctgacaa gcgtaagagc atatatgact
     2341 gtactccagt ccatagagaa ctacgtgcag attgatatca ccagagtatt taataatgtt
     2401 ctccttcaac aaacacaaca cttagacagc catggagaac caaccatcac aagcctgtat
     2461 acaaattggt acttggaaac gttattaaga caagtcagca atggccatat agcttatttt
     2521 cctgcaatga aagcatttgt gaatttaccc acagaaaatg aattaacatt caatgcagag
     2581 gaatactctg atatatcaga aatgaggtcg ttgtcagagc tcttaggccc atatgggatg
     2641 aagttcctaa gcgagagtct tatgtggcat atttcatcac aagttgctga acttaagaaa
     2701 cttgtggtgg agaatgttga tgttctaaca caaatgagga ccagctttga caaaccagac
     2761 cagatggctg cactctttaa aagattatca tctgttgaca gtgtcttgaa aaggatgacg
     2821 ataattggtg ttattttatc cttccggtca ctggcacaag aagcactgag agatgttttg
     2881 tcctaccaca ttccttttct cgtgagctcc attgaggatt tcaaggacca cattccacgg
     2941 gaaaccgata tgaaggttgc aatgaatgtg tatgagctgt catctgctgc tggattacca
     3001 tgcgagattg atcctgcctt agtagtagct ctttcctcac aaaaatcaga gaacattagt
     3061 ccagaagaag aatataagat cgcttgcctc ctgatggtct ttgtagcagt ttccctgcct
     3121 acactggcca gcaatgtgat gtcccagtac agccctgcaa tagaaggaca ctgcaacaat
     3181 attcattgtc tggccaaagc catcaaccaa attgctgctg ctttgtttac aattcacaaa
     3241 ggaagcattg aagacagact taaagaattc ctggcgcttg cgtcctccag tctactgaaa
     3301 atcggccaag agacagataa aactacaacg agaaatagag aatctgttta tttgctactg
     3361 gatatgattg tacaagaatc accgttcctg acaatggatc tcttggaatc ttgttttcct
     3421 tatgtgttgc tgagaaatgc ataccacgct gtctacaaac aaagtgtcac atcctctgca
     3481 tagaatgctc actccagaca agcacgagtt tctgttgcct tgttctatct gtaaactgtg
     3541 gaactatttt accttaaagc ctgaaaacag ttttgtggat taaattttct tcatgctgtt
     3601 gtattttctg atcattggct tcctaacatg gttgtactac aatatatttg gttgattgag
     3661 ttatacattc tcaaaactca gtgaattcta tgattttaga gtatcatttg tttgaactac
     3721 cattaacact atgtttttga taactgataa tgaaaaacct tgcttgttta tttctgaaaa
     3781 agaattgtat ttagtgatta ttttagatag tgatatgata gcacccatct gtgtgtaaat
     3841 tatttcatat agggagagtt ctgatctgta cctacggttc tttctgagat caagactgga
     3901 tgtgcatatc ggtgatgctt acgaatacat ttctacttta ttttgaacat ttgccaaact
     3961 aaatacctaa cactgtttaa ccttagaaat gttagacaac ttgagcattg gaagcagact
     4021 cagtttctca aacagcagct tatgctgttg cctgtagaaa gcctactgat cctctcaccc
     4081 gtgctgcctg gtgggcatct gcactacctt ggaccctgct gtataataaa caaccagact
     4141 cttgtcaact gttgatacag atgttagtcc cgaatcactt tttatatgtt ttaaaatttg
     4201 aaattcaagt gtacctgcca taacataaat aaacactaga ctgtatcaca aaaaaaaaaa
     4261 aaaaaaaaaa
//
