LOCUS       NM_001108216            2550 bp    mRNA    linear   ROD 23-MAR-2023
DEFINITION  Rattus norvegicus NCK adaptor protein 2 (Nck2), mRNA.
ACCESSION   NM_001108216 XM_001059817 XM_237115
VERSION     NM_001108216.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S and Zaidel-Bar R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Mehlitz A, Banhart S, Maurer AP, Kaushansky A, Gordus AG, Zielecki
            J, Macbeath G and Meyer TF.
  TITLE     Tarp regulates early Chlamydia-induced host cell survival through
            interactions with the human adaptor protein SHC1
  JOURNAL   J Cell Biol 190 (1), 143-157 (2010)
   PUBMED   20624904
REFERENCE   3  (bases 1 to 2550)
  AUTHORS   Guan S, Chen M, Woodley D and Li W.
  TITLE     Nckbeta adapter controls neuritogenesis by maintaining the cellular
            paxillin level
  JOURNAL   Mol Cell Biol 27 (17), 6001-6011 (2007)
   PUBMED   17591694
  REMARK    GeneRIF: Results suggest that Nckbeta maintains paxillin stability
            during neuritogenesis.
REFERENCE   4  (bases 1 to 2550)
  AUTHORS   Segura I, Essmann CL, Weinges S and Acker-Palmer A.
  TITLE     Grb4 and GIT1 transduce ephrinB reverse signals modulating spine
            morphogenesis and synapse formation
  JOURNAL   Nat Neurosci 10 (3), 301-310 (2007)
   PUBMED   17310244
REFERENCE   5  (bases 1 to 2550)
  AUTHORS   Latreille M and Larose L.
  TITLE     Nck in a complex containing the catalytic subunit of protein
            phosphatase 1 regulates eukaryotic initiation factor 2alpha
            signaling and cell survival to endoplasmic reticulum stress
  JOURNAL   J Biol Chem 281 (36), 26633-26644 (2006)
   PUBMED   16835242
REFERENCE   6  (bases 1 to 2550)
  AUTHORS   Van Impe K, De Corte V, Eichinger L, Bruyneel E, Mareel M,
            Vandekerckhove J and Gettemans J.
  TITLE     The Nucleo-cytoplasmic actin-binding protein CapG lacks a nuclear
            export sequence present in structurally related proteins
  JOURNAL   J Biol Chem 278 (20), 17945-17952 (2003)
   PUBMED   12637565
REFERENCE   7  (bases 1 to 2550)
  AUTHORS   Benesch S, Lommel S, Steffen A, Stradal TE, Scaplehorn N, Way M,
            Wehland J and Rottner K.
  TITLE     Phosphatidylinositol 4,5-biphosphate (PIP2)-induced vesicle
            movement depends on N-WASP and involves Nck, WIP, and Grb2
  JOURNAL   J Biol Chem 277 (40), 37771-37776 (2002)
   PUBMED   12147689
REFERENCE   8  (bases 1 to 2550)
  AUTHORS   Lee H, Park DS, Wang XB, Scherer PE, Schwartz PE and Lisanti MP.
  TITLE     Src-induced phosphorylation of caveolin-2 on tyrosine 19.
            Phospho-caveolin-2 (Tyr(P)19) is localized near focal adhesions,
            remains associated with lipid rafts/caveolae, but no longer forms a
            high molecular mass hetero-oligomer with caveolin-1
  JOURNAL   J Biol Chem 277 (37), 34556-34567 (2002)
   PUBMED   12091389
REFERENCE   9  (bases 1 to 2550)
  AUTHORS   Gil D, Schamel WW, Montoya M, Sanchez-Madrid F and Alarcon B.
  TITLE     Recruitment of Nck by CD3 epsilon reveals a ligand-induced
            conformational change essential for T cell receptor signaling and
            synapse formation
  JOURNAL   Cell 109 (7), 901-912 (2002)
   PUBMED   12110186
REFERENCE   10 (bases 1 to 2550)
  AUTHORS   Braverman LE and Quilliam LA.
  TITLE     Identification of Grb4/Nckbeta, a src homology 2 and 3
            domain-containing adapter protein having similar binding and
            biological properties to Nck
  JOURNAL   J Biol Chem 274 (9), 5542-5549 (1999)
   PUBMED   10026169
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473965.2.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_237115.4, XM_001059817.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY724492.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132262, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q22"
     gene            1..2550
                     /gene="Nck2"
                     /note="NCK adaptor protein 2"
                     /db_xref="GeneID:316369"
                     /db_xref="RGD:1305965"
     exon            1..87
                     /gene="Nck2"
                     /inference="alignment:Splign:2.1.0"
     exon            88..249
                     /gene="Nck2"
                     /inference="alignment:Splign:2.1.0"
     exon            250..491
                     /gene="Nck2"
                     /inference="alignment:Splign:2.1.0"
     CDS             266..1408
                     /gene="Nck2"
                     /note="non-catalytic region of tyrosine kinase adaptor
                     protein 2"
                     /codon_start=1
                     /product="cytoplasmic protein NCK2"
                     /protein_id="NP_001101686.1"
                     /db_xref="GeneID:316369"
                     /db_xref="RGD:1305965"
                     /translation="MTEEVIVIAKWDYTAQQDQELDIRKNERLWLLDDSKTWWRVRNA
                     ANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKPSARDASPTPSTDAEYPAN
                     GSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSFNGQIGWF
                     PSNYVLEEADEAAAEAPSFLSLRRGTALSNGQGARVLHVVQTLYPFSSVTEEELSFEK
                     GETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPTHTPQISYTGPSA
                     SGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGRNKHFK
                     VQLVDSVYCIGQRRFHSMDELVEHYKKAPIFTSEHGEKLYLVRALQ"
     exon            492..1213
                     /gene="Nck2"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..2550
                     /gene="Nck2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agagcggcat ctgggcgggc gcagcgcggc cgccgccccg ggactagcgc tgctgccctc
       61 cggcctcggc gtgcactgtg cagcgagatc ccagaggttc ccttcctaca gtgtcatccc
      121 ggctggcttg agagctcgga cggtcactgc agctttgtca ccttcaaggt gtcctcagac
      181 agcagaaaaa cctcatcttt ttgaagagtg ctactatttt tccagtctcc ttgtaactgc
      241 ctgccaaaga agggctccat gaaagatgac agaagaagtc attgtcatag ccaagtggga
      301 ctacactgcc cagcaggacc aggagctcga catcaggaag aacgagcggc tgtggctgct
      361 ggacgactcc aagacgtggt ggcgggtgag gaatgcggcc aacaggacag gctacgtgcc
      421 ttccaactac gtggagcgta agaacagcct gaagaagggg tccctggtga agaatctcaa
      481 ggacacgcta ggcctcggga agacgcggag gaagccaagt gctcgggatg cgtccccaac
      541 gccaagcact gatgcggaat acccagccaa tggcagcggt gctgaccgca tctatgacct
      601 caacatccca gccttcgtca agttcgccta cgtagcagaa cgagaggatg agctgtccct
      661 ggtgaaaggc tcccgggtca cagttatgga gaaatgcagt gatggctggt ggcgtggcag
      721 ctttaacggg cagatcggct ggttcccctc caactacgtg ttggaggagg cggacgaggc
      781 tgcagctgag gcccccagct tcctgagcct gcgtcggggc actgcgctaa gcaatgggca
      841 aggtgcacgg gtgctgcacg tggtgcagac gctgtacccc ttcagctctg tcactgagga
      901 ggagcttagt ttcgagaagg gggagaccat ggaggtgatt gagaagccgg agaatgaccc
      961 tgaatggtgg aaatgcaaaa atgcccgagg ccaagtgggc ctggtcccca aaaactatgt
     1021 ggttgtcctc agtgatgggc ctgcactgca ccccacgcac actccacaga tcagctacac
     1081 cgggccctca gccagcgggc gatttgccgg ccgtgagtgg tactatggca acgtgacacg
     1141 gcaccaggcc gagtgtgcgc tcaatgagcg gggcgtcgag ggcgacttcc tcattagaga
     1201 cagcgaatcc tcgcccagtg acttctccgt gtctctcaaa gcatcaggga ggaacaagca
     1261 cttcaaagtg cagctggtgg acagcgtcta ctgcattggg cagcggcggt tccacagcat
     1321 ggacgagctt gtggagcact acaagaaggc ccccatcttc accagcgagc acggggagaa
     1381 gctctacctc gtccgagccc tgcagtgaaa gcagccattg gccccctcat gccctgccca
     1441 ttgtgggcct cgctgccacc tccgcctccc agagcccagc acttctggcc acctccaccc
     1501 atgtggcctg gatcacctct gtggcccagt ctgtccttct tttcagcttt gttggtcaac
     1561 catagctacc taggccctca tagccatgcc catggccacc accttctggt ggtgagggtc
     1621 aaagggagca aagctacgca ctattttcct tttcagttgg aaacggtctg tttgaatctt
     1681 acacacacat acagagagag agagacagag acagagacag agacagagac agagacagag
     1741 acagagaccg gggcttactc agagggcagt gaggctctat gtagtgaata tgtgaccttg
     1801 gcacacactc cgagagctgt gtccatgggt gtttggtgcc tctcatcagc tatgctggtc
     1861 caggccagca gcctgtgtcc tgggcctgct tttttttttt gttgttgttg tttctgtttt
     1921 tgtggctcca ggctctgcag gagtccaaaa atgtgcggat agcaatactg gaaaccctgg
     1981 ggaggtggtg gtgagggaac ttcccagtgc cttggaggct gtgcttgcaa taaaagaggg
     2041 ttttggtgag agtcagtctg caaaagaggg aggtggcaac gcagaaagaa agatactctg
     2101 gtgatgtacc ccaggtgtgc catccacata gtgcttttgc cctttggctt gtttgaattt
     2161 cacaattatg tatttaattc tcaaagtaat atgtatctta gctgtttgtc gacactaata
     2221 agagatgatt aaggaaaaca gctgatctct tgggaagggg agctaccaat actttataca
     2281 cacactatat atatataata tgtgtatatt atatatatat tatttgcttt acagggaaat
     2341 ttttcagggt ttacaagaga atatgtgatt agtagtaaca gaacgtttat gaagaaatta
     2401 cattttcatt tttctttaca tctgaacttc tttacagttt gactatagag tcttgagatg
     2461 gcacattcct acaattgaag aaggggtctt gggatcccct aaacttgcat acccagtttt
     2521 ttgaatattg taataaaaaa aagtattatg
//
