LOCUS       NM_001395185            1681 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus neutrophil cytosolic factor 2 (Ncf2), mRNA.
ACCESSION   NM_001395185 XM_039090951
VERSION     NM_001395185.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Zheleznova,N.N., Yang,C. and Cowley,A.W. Jr.
  TITLE     Role of Nox4 and p67phox subunit of Nox2 in ROS production in
            response to increased tubular flow in the mTAL of Dahl
            salt-sensitive rats
  JOURNAL   Am J Physiol Renal Physiol 311 (2), F450-F458 (2016)
   PUBMED   27279484
  REMARK    GeneRIF: increased mTAL luminal flow results in increases in
            intracellular and mitochondrial H2O2, which are dependent on the
            presence of Nox4, and that p67phox/Nox2 accounts solely for
            increases in O2 (.-) production
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Winiarska,K., Dzik,J.M., Labudda,M., Focht,D., Sierakowski,B.,
            Owczarek,A., Komorowski,L. and Bielecki,W.
  TITLE     Melatonin nephroprotective action in Zucker diabetic fatty rats
            involves its inhibitory effect on NADPH oxidase
  JOURNAL   J Pineal Res 60 (1), 109-117 (2016)
   PUBMED   26514550
  REMARK    GeneRIF: it is concluded that: (i) melatonin beneficial action
            against diabetic nephropathy involves attenuation of the excessive
            activity of Nox; and (ii) the mechanism of melatonin inhibitory
            effect on Nox is based on the mitigation of expression and membrane
            translocation of its regulatory subunits.
REFERENCE   3  (bases 1 to 1681)
  AUTHORS   Sharma,N.M., Rabeler,B., Zheng,H., Raichlin,E. and Patel,K.P.
  TITLE     Exercise Training Attenuates Upregulation of p47(phox) and
            p67(phox) in Hearts of Diabetic Rats
  JOURNAL   Oxid Med Cell Longev 2016, 5868913 (2016)
   PUBMED   26989452
  REMARK    GeneRIF: Exercise Training attenuates the increased expression of
            p47(phox) and p67(phox) in the hearts of diabetic rats which could
            be an underlying mechanism for improving intracardiac matrix and
            thus cardiac function and prevent cardiac remodeling in diabetic
            cardiomyopathy.
REFERENCE   4  (bases 1 to 1681)
  AUTHORS   Salehpour,F., Ghanian,Z., Yang,C., Zheleznova,N.N., Kurth,T.,
            Dash,R.K., Cowley,A.W. Jr. and Ranji,M.
  TITLE     Effects of p67phox on the mitochondrial oxidative state in the
            kidney of Dahl salt-sensitive rats: optical fluorescence 3-D
            cryoimaging
  JOURNAL   Am J Physiol Renal Physiol 309 (4), F377-F382 (2015)
   PUBMED   26062875
  REMARK    GeneRIF: p67(phox) subunit of NADPH oxidase 2 plays an important
            role in the excess production of ROS from mitochondria in the renal
            medulla of the Dahl salt-sensitive rat.
REFERENCE   5  (bases 1 to 1681)
  AUTHORS   Evans,L.C., Ryan,R.P., Broadway,E., Skelton,M.M., Kurth,T. and
            Cowley,A.W. Jr.
  TITLE     Null mutation of the nicotinamide adenine dinucleotide
            phosphate-oxidase subunit p67phox protects the Dahl-S rat from
            salt-induced reductions in medullary blood flow and glomerular
            filtration rate
  JOURNAL   Hypertension 65 (3), 561-568 (2015)
   PUBMED   25489057
  REMARK    GeneRIF: when the p67(phox)subunit of nicotinamide adenine
            dinucleotide phosphate-oxidase is not functional in the
            salt-sensitive rats, high salt diet does not cause reduced renal
            medullary blood flow and salt-sensitive hypertension is attenuated
REFERENCE   6  (bases 1 to 1681)
  AUTHORS   Grogan,A., Reeves,E., Keep,N., Wientjes,F., Totty,N.F.,
            Burlingame,A.L., Hsuan,J.J. and Segal,A.W.
  TITLE     Cytosolic phox proteins interact with and regulate the assembly of
            coronin in neutrophils
  JOURNAL   J Cell Sci 110 (Pt 24), 3071-3081 (1997)
   PUBMED   9365277
REFERENCE   7  (bases 1 to 1681)
  AUTHORS   Fazal,N., Sabeh,F., Gamelli,R.L. and Sayeed,M.M.
  TITLE     Elevated expression of p47phox and p67phox proteins in neutrophils
            from burned rats
  JOURNAL   Shock 8 (4), 256-260 (1997)
   PUBMED   9329126
REFERENCE   8  (bases 1 to 1681)
  AUTHORS   Wientjes,F.B., Hsuan,J.J., Totty,N.F. and Segal,A.W.
  TITLE     p40phox, a third cytosolic component of the activation complex of
            the NADPH oxidase to contain src homology 3 domains
  JOURNAL   Biochem J 296 (Pt 3) (Pt 3), 557-561 (1993)
   PUBMED   8280052
REFERENCE   9  (bases 1 to 1681)
  AUTHORS   Gout,I., Dhand,R., Hiles,I.D., Fry,M.J., Panayotou,G., Das,P.,
            Truong,O., Totty,N.F., Hsuan,J., Booker,G.W. et al.
  TITLE     The GTPase dynamin binds to and is activated by a subset of SH3
            domains
  JOURNAL   Cell 75 (1), 25-36 (1993)
   PUBMED   8402898
REFERENCE   10 (bases 1 to 1681)
  AUTHORS   Francke,U., Hsieh,C.L., Foellmer,B.E., Lomax,K.J., Malech,H.L. and
            Leto,T.L.
  TITLE     Genes for two autosomal recessive forms of chronic granulomatous
            disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1)
  JOURNAL   Am J Hum Genet 47 (3), 483-492 (1990)
   PUBMED   2393022
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On Jun 8, 2021 this sequence version replaced XM_039090951.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: JN864042.1, JN864041.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-220               JAXUCZ010000013.1  67505492-67505711
            221-303             JAXUCZ010000013.1  67508009-67508091
            304-412             JAXUCZ010000013.1  67515072-67515180
            413-547             JAXUCZ010000013.1  67519291-67519425
            548-655             JAXUCZ010000013.1  67521949-67522056
            656-715             JAXUCZ010000013.1  67524294-67524353
            716-759             JAXUCZ010000013.1  67525602-67525645
            760-901             JAXUCZ010000013.1  67528065-67528206
            902-970             JAXUCZ010000013.1  67528386-67528454
            971-1046            JAXUCZ010000013.1  67529332-67529407
            1047-1072           JAXUCZ010000013.1  67531172-67531197
            1073-1224           JAXUCZ010000013.1  67531645-67531796
            1225-1336           JAXUCZ010000013.1  67531948-67532059
            1337-1514           JAXUCZ010000013.1  67533302-67533479
            1515-1681           JAXUCZ010000013.1  67535849-67536015
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q21"
     gene            1..1681
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /note="neutrophil cytosolic factor 2"
                     /db_xref="GeneID:364018"
                     /db_xref="RGD:1309424"
     exon            1..220
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..1627
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /note="neutrophil NADPH oxidase factor 2; 67 kDa
                     neutrophil oxidase factor"
                     /codon_start=1
                     /product="neutrophil cytosol factor 2"
                     /protein_id="NP_001382114.1"
                     /db_xref="GeneID:364018"
                     /db_xref="RGD:1309424"
                     /translation="MSLAEAIRLWNEGVQAADKKDWKGALEAFSEVQDPHSRICFNIG
                     CMYTILDNLQEAEQAFTKSINRDKHLAVAYFQRGMLYYSMEKYDLAIKDLKEALTQLR
                     GNQLIDYKILGLQFKLFACEVLYNIALMHAKKEEWKKAEEQLALATNMKSEPRHSKID
                     KAMESIWKQKLFEPVVIPVGRLFRPNERQVAQLAKKDYLGKATVVASVVHQDNFSGFA
                     PLQPQSAEPPPRPKTPEIFRALEGEAHRVLFGFVPETPEELQVMPGNIVFVLKKGSDN
                     WATVMFNGQKGLVPCNYLEPVELRIHPQSQPQEDTSLESDIPPPPNSSPPERLQLSPG
                     HKQKEEPKEVKRSVPMPYMLKVHYKYTVVMETQLGLPYSQLRNMVSKKLELLPEHTKL
                     SYQRRDSPELLLLSEESMKDAWAQVKNYCLTLWCEHTVGDQGFVDEPKEKENSDADNR
                     TTEPQPKEGTQVVAIFSYDATQPEDLEFVEGDVILVLSHVNEEWLEGECKGKIGIFPK
                     AFVEGCAAKNLEGTPREV"
     exon            221..303
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     exon            304..412
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     exon            413..547
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     exon            548..655
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     exon            656..715
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     exon            716..759
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     exon            760..901
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     exon            902..970
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1046
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1072
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1224
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1336
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1514
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1681
                     /gene="Ncf2"
                     /gene_synonym="NCF-2; p67phox"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agagacacct tgaactacca tcctgtcttc tagtaagcac ctaaccatgt ccctagctga
       61 ggccatcagg ctctggaatg agggggtgca ggcagccgac aagaaggact ggaagggtgc
      121 cttggaggcc ttcagcgagg tgcaggaccc ccactcgagg atttgcttca acataggctg
      181 catgtacacc atcctggaca acctgcagga ggctgagcag gccttcacca aaagcatcaa
      241 cagagacaag cacttggcag tggcttactt ccagcgagga atgctctact acagcatgga
      301 gaaatatgac cttgctatca aagaccttaa agaggccttg actcagcttc gagggaatca
      361 gctgatagac tacaagatcc tggggctgca gttcaagctg tttgcctgtg aggtgttgta
      421 taacatcgct ctcatgcatg ccaagaaaga ggaatggaaa aaagcagaag agcagttagc
      481 attggcaaca aacatgaagt ctgagcccag gcattccaag attgacaagg ccatggaaag
      541 catctggaag cagaagctgt tcgagccggt ggtgatccct gtgggtaggc tgttccgtcc
      601 aaatgagagg caggtggctc agctggccaa gaaggactat ctgggcaagg ctacggttgt
      661 agcctctgtg gttcaccaag acaatttttc tggcttcgcc cctctgcagc cacagtcagc
      721 agagcctccg cccagaccca aaaccccaga gatcttcagg gctctggaag gcgaggcaca
      781 tcgtgtgttg tttggctttg tgccggagac gccggaagaa ctacaggtca tgcccgggaa
      841 catcgtcttt gtcttgaaga agggcagtga taactgggcc accgtcatgt tcaacggaca
      901 gaaggggctt gtcccttgca actacctgga gcccgttgag cttcgaattc accctcagtc
      961 gcagcctcag gaagatacct ccctggaatc cgatattcca ccacctccta actctagtcc
     1021 cccagaaaga ctccagttgt caccaggtca caagcaaaaa gaagagccta aggaagtgaa
     1081 gcgcagcgtg cccatgcctt acatgctcaa ggtgcattac aaatacacag tggtcatgga
     1141 gactcagctt ggcctcccct acagccagct tcggaacatg gtgtctaaga agctggagct
     1201 cttgccagaa cacactaaac tgagctacca gcgtcgggac agcccggagc tgctgctcct
     1261 gtcagaagaa agcatgaagg atgcctgggc ccaagtgaaa aactactgcc tgaccctctg
     1321 gtgtgagcac acagtgggtg atcaaggttt tgtagatgaa cccaaggaaa aggaaaactc
     1381 tgatgccgat aaccggacaa cagagcctca gcctaaggaa gggacccagg tggtagcgat
     1441 cttcagttat gatgctaccc agccagaaga cctggagttt gtggaaggag atgtgatctt
     1501 ggtgctatca catgtgaatg aagaatggct ggaaggggag tgtaaaggga aaattggcat
     1561 tttccctaaa gcttttgttg aaggatgtgc agccaagaat ttggaaggca ctcccagaga
     1621 agtataggat gctctacaat ctatgaagct gaagaaaagg gtggtttttt gttttgtttt
     1681 g
//
