LOCUS       NM_031653               5201 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus neurocan (Ncan), mRNA.
ACCESSION   NM_031653 XM_346487
VERSION     NM_031653.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Yui,S., Fujita,N., Chung,C.S., Morita,M. and Nishimura,R.
  TITLE     Olfactory ensheathing cells (OECs) degrade neurocan in injured
            spinal cord by secreting matrix metalloproteinase-2 in a rat
            contusion model
  JOURNAL   Jpn J Vet Res 62 (4), 151-162 (2014)
   PUBMED   25597185
  REMARK    GeneRIF: In vitro results showed that MMP-2 was the dominant MMP
            expressed by olfactory ensheathing cells (OECs). In vivo results
            revealed that transplanted OECs secreted MMP-2 in injured spinal
            cord and that the transplantation decreased neurocan expression.
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Sajad,M., Zargan,J., Chawla,R., Umar,S. and Khan,H.A.
  TITLE     Upregulation of CSPG3 accompanies neuronal progenitor proliferation
            and migration in EAE
  JOURNAL   J Mol Neurosci 43 (3), 531-540 (2011)
   PUBMED   21107918
  REMARK    GeneRIF: Results report for the first time that extracellular
            matrix chondroitin sulphate proteoglycan, CSPG3 (neurocan) is
            upregulated after primary inflammatory injury.
REFERENCE   3  (bases 1 to 5201)
  AUTHORS   Shen,L.H., Li,Y., Gao,Q., Savant-Bhonsale,S. and Chopp,M.
  TITLE     Down-regulation of neurocan expression in reactive astrocytes
            promotes axonal regeneration and facilitates the neurorestorative
            effects of bone marrow stromal cells in the ischemic rat brain
  JOURNAL   Glia 56 (16), 1747-1754 (2008)
   PUBMED   18618668
  REMARK    GeneRIF: Our data suggest that BMSCs promote axonal regeneration by
            reducing neurocan expression in peri-infarct astrocytes.
REFERENCE   4  (bases 1 to 5201)
  AUTHORS   Deepa,S.S., Carulli,D., Galtrey,C., Rhodes,K., Fukuda,J.,
            Mikami,T., Sugahara,K. and Fawcett,J.W.
  TITLE     Composition of perineuronal net extracellular matrix in rat brain:
            a different disaccharide composition for the net-associated
            proteoglycans
  JOURNAL   J Biol Chem 281 (26), 17789-17800 (2006)
   PUBMED   16644727
REFERENCE   5  (bases 1 to 5201)
  AUTHORS   Cheng,D., Hoogenraad,C.C., Rush,J., Ramm,E., Schlager,M.A.,
            Duong,D.M., Xu,P., Wijayawardana,S.R., Hanfelt,J., Nakagawa,T.,
            Sheng,M. and Peng,J.
  TITLE     Relative and absolute quantification of postsynaptic density
            proteome isolated from rat forebrain and cerebellum
  JOURNAL   Mol Cell Proteomics 5 (6), 1158-1170 (2006)
   PUBMED   16507876
REFERENCE   6  (bases 1 to 5201)
  AUTHORS   Friedlander,D.R., Milev,P., Karthikeyan,L., Margolis,R.K.,
            Margolis,R.U. and Grumet,M.
  TITLE     The neuronal chondroitin sulfate proteoglycan neurocan binds to the
            neural cell adhesion molecules Ng-CAM/L1/NILE and N-CAM, and
            inhibits neuronal adhesion and neurite outgrowth
  JOURNAL   J Cell Biol 125 (3), 669-680 (1994)
   PUBMED   7513709
REFERENCE   7  (bases 1 to 5201)
  AUTHORS   Grumet,M., Milev,P., Sakurai,T., Karthikeyan,L., Bourdon,M.,
            Margolis,R.K. and Margolis,R.U.
  TITLE     Interactions with tenascin and differential effects on cell
            adhesion of neurocan and phosphacan, two major chondroitin sulfate
            proteoglycans of nervous tissue
  JOURNAL   J Biol Chem 269 (16), 12142-12146 (1994)
   PUBMED   7512960
REFERENCE   8  (bases 1 to 5201)
  AUTHORS   Rauch,U., Karthikeyan,L., Maurel,P., Margolis,R.U. and
            Margolis,R.K.
  TITLE     Cloning and primary structure of neurocan, a developmentally
            regulated, aggregating chondroitin sulfate proteoglycan of brain
  JOURNAL   J Biol Chem 267 (27), 19536-19547 (1992)
   PUBMED   1326557
REFERENCE   9  (bases 1 to 5201)
  AUTHORS   Rauch,U., Gao,P., Janetzko,A., Flaccus,A., Hilgenberg,L.,
            Tekotte,H., Margolis,R.K. and Margolis,R.U.
  TITLE     Isolation and characterization of developmentally regulated
            chondroitin sulfate and chondroitin/keratan sulfate proteoglycans
            of brain identified with monoclonal antibodies
  JOURNAL   J Biol Chem 266 (22), 14785-14801 (1991)
   PUBMED   1907283
REFERENCE   10 (bases 1 to 5201)
  AUTHORS   Hobbs,A.A. and Rosen,J.M.
  TITLE     Sequence of rat alpha- and gamma-casein mRNAs: evolutionary
            comparison of the calcium-dependent rat casein multigene family
  JOURNAL   Nucleic Acids Res 10 (24), 8079-8098 (1982)
   PUBMED   6298707
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000016.1.
            
            On Sep 12, 2012 this sequence version replaced NM_031653.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M97161.1, SRR26643298.71098.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-69                JAXUCZ010000016.1  19335907-19335975
            70-149              JAXUCZ010000016.1  19340264-19340343
            150-548             JAXUCZ010000016.1  19341273-19341671
            549-723             JAXUCZ010000016.1  19344435-19344609
            724-851             JAXUCZ010000016.1  19344706-19344833
            852-1145            JAXUCZ010000016.1  19345137-19345430
            1146-1742           JAXUCZ010000016.1  19346692-19347288
            1743-2936           JAXUCZ010000016.1  19348606-19349799
            2937-3053           JAXUCZ010000016.1  19353280-19353396
            3054-3167           JAXUCZ010000016.1  19354822-19354935
            3168-3326           JAXUCZ010000016.1  19355348-19355506
            3327-3409           JAXUCZ010000016.1  19356636-19356718
            3410-3554           JAXUCZ010000016.1  19359031-19359175
            3555-3737           JAXUCZ010000016.1  19359714-19359896
            3738-5201           JAXUCZ010000016.1  19360908-19362371
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16p14"
     gene            1..5201
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /note="neurocan"
                     /db_xref="GeneID:58982"
                     /db_xref="RGD:619941"
     exon            1..69
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     exon            70..149
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..3868
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /note="neurocan core protein; 245 kDa early postnatal core
                     glycoprotein; chondroitin sulfate proteoglycan 3; neurocan
                     core protein-like"
                     /codon_start=1
                     /product="neurocan core protein precursor"
                     /protein_id="NP_113841.2"
                     /db_xref="GeneID:58982"
                     /db_xref="RGD:619941"
                     /translation="MGAESVWASGLLVLWLLLLVSGDQDTQDTTTTEKGLHMLKSGSG
                     PIQAALAELVALPCFFTLQPRQSPLGDIPRIKWTKVQTASGQRQDLPILVAKDNVVRV
                     AKGWQGRVSLPAYPRHRANATLLLGPLRASDSGLYRCQVVKGIEDEQDLVTLEVTGVV
                     FHYRAARDRYALTFAEAQEACHLSSATIAAPRHLQAAFEDGFDNCDAGWLSDRTVRYP
                     ITQSRPGCYGDRSSLPGVRSYGRRDPQELYDVYCFARELGGEVFYVGPARRLTLAGAR
                     ALCQRQGAALASVGQLHLAWHEGLDQCDPGWLADGSVRYPIQTPRRRCGGSAPGVRTV
                     YRFANRTGFPAPGARFDAYCFRAHHHTPQRGDSEIPSSGDEGEIVSAEGPPAPELKPR
                     LGEQEVITPDFQEPLVSSGEDEPLDLTRTQASQETLASTPGGPTLASWPSREKWLLTG
                     VTSSTGVPSPSSLGVDMEETTPSGTQVAPTPTMRRGRFKGLNGRHFQQQGPEDQLLEA
                     AEASAQPPTLEVTADHMGPSAATEALESDQSHSPWAILTNEVDVPGAGSLGSRSLPES
                     RKWSPSLISPSTVPSTDSTPGLKPGADEAPGVKSAIHHPPWLPSEPAVPSSIPSEALS
                     AVSLQASPGDGSPDFPIVAMLRAPKLWLLPHSTLVPNVSPIPLSPASPLPSSVPEEQA
                     VRPVSFGAEDPETPFQTTMAAPGEASHGSPEADSIEIEGISSMQATKHPISGPWASLD
                     SSNVTVNPVPSDAGILGTESGVLDLPGSPTSDGQATVDKVLATWLPLPGHGLDTGSQS
                     TPMEAHGVTMSVEPTVALEGGATKDPMEATMDVVPSTVDATSGSEPKSSISSTHVVVT
                     AAGDQGTPTLTPTSSEGQVVAQESLGTLTSLPSHPWSSLASSMDEVASVSSGEPTGLW
                     DIPSTLIPVSLGLDESDLKVVAESPGLEGFWEEVASGQEDPTDPCENNPCLHGGTCRT
                     NGTMYGCSCDQGYAGENCEIDIDDCLCSPCENGGTCIDEVNGFICLCLPSYGGNLCEK
                     DTEGCDRGWHKFQGHCYRYFAHRRAWEDAERDCRRRAGHLTSVHSPEEHKFINSFGHE
                     NSWIGLNDRTVERDFQWTDNTGLQYENWREKQPDNFFAGGEDCVVMVAHENGRWNDVP
                     CNYNLPYVCKKGTVLCGPPPAVENASLVGVRKVKYNVHATVRYQCDEGFSQHHVATIR
                     CRNNGKWDRPQIVCTKPRRSHRMRRHHHHPHRHHKPRKEHRKHKRHPAEDWEKDEGDF
                     C"
     exon            150..548
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     exon            549..723
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     exon            724..851
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     exon            852..1145
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1742
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..2936
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     exon            2937..3053
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     exon            3054..3167
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     exon            3168..3326
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     exon            3327..3409
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     exon            3410..3554
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     exon            3555..3737
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
     exon            3738..5201
                     /gene="Ncan"
                     /gene_synonym="Cspg3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aacaccggag ccaggcgtcg cgtcctttgt gcccggaacc gtggggatgt gtccgcgcta
       61 aggagccagc tccagtatgg gggccgaatc tgtctgggcc tcaggcctcc tggtgctgtg
      121 gctgcttctc ctagtgtctg gggatcagga cacacaggac accaccacca cggaaaaggg
      181 gcttcacatg ctgaagtcgg ggtcaggacc catccaggct gctttggcag agttagtggc
      241 cctgccctgc ttctttaccc ttcaaccacg gcaaagcccc ctgggagaca ttcctcggat
      301 caagtggacg aaagttcaga ctgcatcagg ccagcgacag gatttgccaa tcttggtggc
      361 caaagacaac gtggtgcgtg tggccaaggg ctggcaggga cgggtgtcat tgcctgccta
      421 tccccggcac agagccaatg ctacacttct gttggggcca ctgcgagcca gtgactctgg
      481 gctgtatcgc tgccaagtgg tgaagggtat cgaggatgag caggacctgg taaccctgga
      541 agtgacaggc gtcgtgttcc attatcgggc ggcccgggac cgctatgcgt tgaccttcgc
      601 tgaggcccag gaggcttgtc acctgagctc cgctaccatt gcggctccaa ggcacctgca
      661 ggctgcgttc gaagatggct ttgacaactg cgatgcgggc tggctctcag accgcacggt
      721 ccggtacccg atcactcagt cgcgtcccgg ttgctatggt gatcgcagca gcctgccagg
      781 tgtccggagc tacgggagac gcgacccgca ggaactctac gatgtctact gctttgcccg
      841 cgagctaggg ggtgaagtct tttacgtggg ccccgcccgc cgactgaccc tggcgggggc
      901 gcgggcactg tgtcagcgtc agggtgcagc gctggcctcc gtgggacagt tgcacctggc
      961 ctggcacgag ggcctggacc agtgcgaccc gggctggctg gcagacggca gcgtgcgcta
     1021 cccgatccag actccacggc ggcgttgcgg gggctccgct ccaggtgtgc gcacagtgta
     1081 ccgcttcgcc aaccgcactg gctttcctgc gccaggagcc cgcttcgacg cctactgctt
     1141 ccgagctcat caccatacac cacaacgtgg agactccgag atcccctcat ctggagatga
     1201 gggggagatt gtgtcagcag aggggccgcc agccccagaa ctaaagccca gattggggga
     1261 gcaggaggtg ataacacctg acttccagga acctctcgta tccagtggag aagatgaacc
     1321 cctagatttg acaaggacac aagcatctca ggagacgctc gcctctaccc cagggggtcc
     1381 cacactggct tcatggccct ctcgggaaaa gtggctgctt acaggtgtca caagctccac
     1441 gggtgtcccc agccccagca gcctgggagt agacatggaa gagacaacac cctcaggcac
     1501 acaggtagcc cccaccccca caatgaggag gggccgcttt aaagggttga atggtcgaca
     1561 cttccagcaa cagggcccag aagaccagct tctggaggca gcagaggcca gtgcccagcc
     1621 tcccaccctg gaagttactg ctgaccacat ggggccttct gcagccacag aggccttgga
     1681 gagtgaccag agccacagtc cttgggccat tctgaccaat gaagtggatg tgccaggggc
     1741 aggctctctt ggcagcagga gtctcccaga gtcccggaag tggtccccgt cgctgatttc
     1801 acccagtact gtcccgagca ctgacagtac tcctggcctg aagccagggg cagatgaggc
     1861 ccctggtgtg aagtcagcca tccaccaccc accctggttg ccctcagaac ccgctgtccc
     1921 atcctccatt ccctcagagg ccctaagtgc tgtctcccta caggcatccc ctggtgatgg
     1981 ctctccagac ttccccattg tagccatgct tcgagccccc aaactgtggc ttctgccaca
     2041 ctctacactc gtcccgaatg tgtccccaat cccactctcc ccagcttctc cactcccctc
     2101 ctcggtccca gaagaacagg ctgtcagacc tgtcagcttt ggagcagaag accccgagac
     2161 cccatttcag accaccatgg ctgccccagg tgaagccagc cacggatccc ctgaggcaga
     2221 ctccatagaa atcgagggga tcagctccat gcaggctaca aagcacccca tctctggccc
     2281 atgggcttct ttggactcca gtaatgtgac agtgaatcct gtcccttctg atgctggcat
     2341 cctagggact gagtctgggg tcttggactt accagggagt cccacatcag acggacaggc
     2401 cactgtggac aaggtgctgg ccacctggct accactgcct ggccacggac tggacactgg
     2461 ctcccagtcc acacccatgg aagcccatgg agtaaccatg agtgtggaac ctacagtggc
     2521 tttggaagga ggtgccacca aagacccaat ggaggccacc atggatgtgg tccccagcac
     2581 tgttgatgcc acttcggggt ctgaacccaa aagttccatt tctagcaccc atgtggttgt
     2641 gactgcagct ggggaccagg gcacacccac actgacccct acaagctctg aaggtcaggt
     2701 ggtggcccag gagtcactgg gaaccctcac cagtctgcct tctcatccct ggtcatctct
     2761 ggcctccagc atggacgaag tggcctcggt ttcctcagga gaacccacag ggttgtggga
     2821 catccccagc actctgatac ctgtgtcctt gggcttggat gaatcagacc tgaaggttgt
     2881 ggctgagagc ccaggcttgg agggcttctg ggaagaggtg gccagtgggc aggaagaccc
     2941 cacggatccc tgcgagaaca acccttgtct gcacgggggc acctgccgca caaatggcac
     3001 catgtacggc tgtagttgtg atcagggcta tgctggggag aattgtgaaa ttgacattga
     3061 tgactgcttg tgcagccctt gtgagaatgg gggtacctgc attgatgagg tgaatggttt
     3121 catctgcctc tgtctcccca gctatggggg caacctgtgc gagaaggaca cagaaggatg
     3181 cgaccgtggc tggcacaaat tccagggcca ctgctaccgc tactttgctc atcggcgggc
     3241 ctgggaggac gcagagagag actgcaggcg ccgagccggc cacctgacaa gtgtccactc
     3301 cccagaagag cacaagttta ttaacagttt tggacacgag aattcatgga ttggcctgaa
     3361 tgacaggaca gtagagaggg acttccagtg gacagacaac acaggactgc aatatgagaa
     3421 ctggagagag aagcagccgg ataatttctt cgcaggtggg gaggattgtg tggtgatggt
     3481 ggcgcatgag aatggacgct ggaatgatgt cccctgtaac tacaacctcc cctacgtctg
     3541 caagaagggt acagtgctgt gtgggccccc tccagcagtg gagaatgcct ctcttgttgg
     3601 tgtgcgcaag gtcaagtaca atgtccatgc cactgtgcga taccagtgtg atgaaggatt
     3661 ctcccagcac catgtggcta ccatccgatg ccgaaacaat gggaagtggg accggcctca
     3721 gattgtgtgc accaagccca ggcggtcaca tcggatgcgt cgacaccacc accatccaca
     3781 ccggcatcac aagccacgca aggagcacag aaaacacaag agacacccag cggaagactg
     3841 ggagaaagat gaaggggatt tctgctaacg atccagacta atcaagcaca agctcccaca
     3901 cctcctccag agcattcccc tggggaccca gaacccagac agccaccaga gagagggtgg
     3961 ggacaccctg gagccccaca cccctgcagt cggtcctctg tacaaagctc agatcaccct
     4021 ctccttcctt actgaggtcc tcctggcagg ggtggcggga cctgagaggc cattctggtc
     4081 tggctgagcc cttaggatta cttcccagct gtcaaaggga gtctcgaagt ctgcatccat
     4141 gaggggcaaa cagtatgtct gtgggcgctg ggtagtgact ttcacaccag agattcaggc
     4201 ttcgtaaaca gcggactata ctgaatccat ggcaaacctt tggttaaagt cactgtattt
     4261 gagtggagga ccagcagagc aaacaggatg ctctgtggtc ccctgggatc ccctaagggc
     4321 aggcttccat ccctccgtct ttcttttttc cctttctcct tcctccctct caattccaac
     4381 actgccaggc ctcaacctag gctggccttg aactgactat tgtagaagga tgtccttgaa
     4441 gtctcaatcc tcctgcccct gtgccaccat gcctacctgg tttatttggt gctggacatg
     4501 gaacccagcg ctgggcaagt gctctctcta ctaaccagct ctggtctttg gtcttggtgc
     4561 agaccactaa atctagtgtg gaactcttgt atattgaatt ttagtgatgt ctcttagcct
     4621 ggcctccgtg gcttcctagg ggaggttcct cagagcaatg ggggtgggga gggttactct
     4681 attgctgact ccccacgtct agtgcagcgc tgagatctgg tggttctatt ttgggttgtt
     4741 tttgttttga gacagggcct cactgtgcag ctctagctgg cctggaactt gctatgtaga
     4801 ccaggctagc ctggaactca gcgatctgtc tgcctctgcc tctctagtta ctatgcccag
     4861 ccttcttttg ttttactgac ctaggatctc tatagcccag gctgggtttc agtccacagc
     4921 gatcagatcc tttctcaacc acctaagagc tgaggtgaca ggcacaccat cacgtgtggc
     4981 acttttgtgg gtgtgtgatg gcaaatctcc cacacccatg tgtccagacg tgagacagca
     5041 gagtagagtg tactggatgc tgctgtatcc ctctcctcca tctctcaggg agacagacaa
     5101 ccttctgccc ggaccaagat gctgccatgt tttctaaccc agagcctgtc tttactaaaa
     5161 ccctctcagc agacctcagt aaatctcctg cctcagatcc c
//
