LOCUS       NM_031670               1434 bp    mRNA    linear   ROD 22-MAR-2023
DEFINITION  Rattus norvegicus napsin A aspartic peptidase (Napsa), mRNA.
ACCESSION   NM_031670
VERSION     NM_031670.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Niderla-Bielinska J and Moskalewski S.
  TITLE     Involucrin, but not filaggrin and Kdap mRNA, expression is
            downregulated in 3-D cultures of intact rat hair bulbs after
            calcium stimulation
  JOURNAL   Folia Histochem Cytobiol 49 (2), 335-343 (2011)
   PUBMED   21744336
  REMARK    GeneRIF: The level of mRNA for differentiation markers: involucrin,
            filaggrin, keratinocyte differentiation associated protein and
            trichohyalin, was studied by RealTime PCR.
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta
            R, Wang NS and Knepper MA.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   3  (bases 1 to 1434)
  AUTHORS   Woischnik M, Bauer A, Aboutaam R, Pamir A, Stanzel F, de Blic J and
            Griese M.
  TITLE     Cathepsin H and napsin A are active in the alveoli and increased in
            alveolar proteinosis
  JOURNAL   Eur Respir J 31 (6), 1197-1204 (2008)
   PUBMED   18216060
REFERENCE   4  (bases 1 to 1434)
  AUTHORS   Ueno T, Linder S, Na CL, Rice WR, Johansson J and Weaver TE.
  TITLE     Processing of pulmonary surfactant protein B by napsin and
            cathepsin H
  JOURNAL   J Biol Chem 279 (16), 16178-16184 (2004)
   PUBMED   14766755
REFERENCE   5  (bases 1 to 1434)
  AUTHORS   Schauer-Vukasinovic V, Wright MB, Breu V and Giller T.
  TITLE     Cloning, expression and functional characterization of rat napsin
  JOURNAL   Biochim Biophys Acta 1492 (1), 207-210 (2000)
   PUBMED   10858550
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB809217.1 and BC078790.1.
            
            On Dec 7, 2007 this sequence version replaced NM_031670.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC078790.1, FQ230345.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 CB809217.1         27-34
            9-1434              BC078790.1         1-1426
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q22"
     gene            1..1434
                     /gene="Napsa"
                     /gene_synonym="Kdap"
                     /note="napsin A aspartic peptidase"
                     /db_xref="GeneID:60575"
                     /db_xref="RGD:61940"
     exon            1..116
                     /gene="Napsa"
                     /gene_synonym="Kdap"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..1302
                     /gene="Napsa"
                     /gene_synonym="Kdap"
                     /EC_number="3.4.23.5"
                     /note="kidney-derived aspartic protease-like protein;
                     napsin-A"
                     /codon_start=1
                     /product="napsin-A precursor"
                     /protein_id="NP_113858.1"
                     /db_xref="GeneID:60575"
                     /db_xref="RGD:61940"
                     /translation="MLPPPLLLLLCLVLLGNLEPAATLIRVPLRRIHPGHRIFSPLYG
                     WEQRAELSRTPTSGGKTAFVPLSKFMNTQYFGDIGLGTPPQNFTVVFDTGSSNLWVPS
                     TRCHFFSLACWFHHRFNPKASSSFRPNGTKFAIQYGTGRLSGILSRDNLTIGGIHNVS
                     VTFGEALWEPSLVFALARFDGILGLGFPTLAVGGVQPPLDALVEQRLLEKPVFSFYLN
                     RDSEGSDGGELVLGGSDPDHYVPPLTFIPVTIPAYWQVHMQSVKVGTGLNLCAQGCGA
                     ILDTGTSLITGPSEEIRALNKAVGGFPLLTGQYLIQCSKIPELPTVSFSLGGVWFNLT
                     GQDYVIKILQSDVGLCLLGFQALDIPKPEGPLWILGDVFLGSYVAVFDRGDKNIGPRV
                     GLARARSRATGRAERKATQAQFLRRRPG"
     exon            117..252
                     /gene="Napsa"
                     /gene_synonym="Kdap"
                     /inference="alignment:Splign:2.1.0"
     exon            253..376
                     /gene="Napsa"
                     /gene_synonym="Kdap"
                     /inference="alignment:Splign:2.1.0"
     exon            377..495
                     /gene="Napsa"
                     /gene_synonym="Kdap"
                     /inference="alignment:Splign:2.1.0"
     exon            496..695
                     /gene="Napsa"
                     /gene_synonym="Kdap"
                     /inference="alignment:Splign:2.1.0"
     exon            696..818
                     /gene="Napsa"
                     /gene_synonym="Kdap"
                     /inference="alignment:Splign:2.1.0"
     exon            819..963
                     /gene="Napsa"
                     /gene_synonym="Kdap"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1062
                     /gene="Napsa"
                     /gene_synonym="Kdap"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1366
                     /gene="Napsa"
                     /gene_synonym="Kdap"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagccacagg acatcttggc ccagtcctag tccccagaga tgttgccacc accgctgctg
       61 ctgctgctgt gcctggtgct gctggggaat ttggagcctg cggccacact gatccgagtc
      121 cctcttcgcc gaatccaccc tggacacagg atcttcagcc cactgtatgg atgggaacag
      181 agagcagagc tttctaggac ccccacctcc ggcggcaaga ccgcctttgt gcctctatcc
      241 aagttcatga acacccagta ttttggagac attggtttgg gaacgcctcc tcagaatttc
      301 acagttgtct ttgacacggg ttcttccaac ttgtgggttc catccacgag atgtcatttc
      361 ttcagtttgg cctgctggtt ccaccatcgt tttaatccca aggcctccag ctcctttcgg
      421 cccaatggga ccaagtttgc cattcagtat gggaccgggc ggctgagtgg aatcctgagc
      481 cgggataatc tgactatcgg cggtatccac aatgtttctg tgacctttgg agaggctctg
      541 tgggagccca gcctggtctt tgctttggcc cgctttgatg ggatcctggg cctcggcttc
      601 cccactctgg ctgtgggagg agttcagcct ccactggacg cactggtgga gcagcgactg
      661 ctggagaaac ccgtcttctc cttttacctc aacagggatt ctgaagggtc tgatggggga
      721 gagctggtcc tggggggctc ggacccggat cactacgtac ctcccctcac cttcattcca
      781 gtcaccatcc ctgcctactg gcaggtccac atgcagagtg tgaaggttgg cacggggctg
      841 aatctctgcg cccagggctg tggtgccatc ctagacacag gcacatccct catcacggga
      901 cccagtgagg agatccgggc cttgaataag gccgttgggg gatttcccct cctgaccggg
      961 cagtacctca ttcagtgttc caagatccca gagctcccca ctgtctcctt ctccctcggt
     1021 ggagtctggt ttaacctcac aggtcaggac tatgtcatca agattcttca gagtgatgtt
     1081 ggcctctgcc tgttgggctt ccaagccttg gacatcccca agcctgaagg acccctttgg
     1141 atcctcgggg acgtcttttt ggggtcctat gtggccgtct ttgaccgagg ggacaaaaac
     1201 atcggcccgc gcgtgggact ggcgcgtgct cggtctcgtg caacaggacg ggcagaaaga
     1261 aaggctacgc aagcgcagtt cctcagaaga cgccctggtt agggtacata cacacagggc
     1321 cacagcagct atgcttcttt ccaataaaaa agaaaaatca acttttaaaa aaaaaaaaaa
     1381 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//
