LOCUS       NM_022178               5608 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Rattus norvegicus myosin VA (Myo5a), transcript variant 2, mRNA.
ACCESSION   NM_022178
VERSION     NM_022178.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Canclini,L., Cal,K., Bardier,C., Ruiz,P., Mercer,J.A. and
            Calliari,A.
  TITLE     Calcium triggers the dissociation of myosin-Va from ribosomes in
            ribonucleoprotein complexes
  JOURNAL   FEBS Lett 594 (14), 2311-2321 (2020)
   PUBMED   32412091
  REMARK    GeneRIF: Calcium triggers the dissociation of myosin-Va from
            ribosomes in ribonucleoprotein complexes.
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Maschi,D., Gramlich,M.W. and Klyachko,V.A.
  TITLE     Myosin V functions as a vesicle tether at the plasma membrane to
            control neurotransmitter release in central synapses
  JOURNAL   Elife 7, e39440 (2018)
   PUBMED   30320552
  REMARK    GeneRIF: myosin V acts as a vesicle tether at the plasma membrane,
            rather than a motor transporting vesicles to the release sites, or
            directly regulating vesicle exocytosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5608)
  AUTHORS   Landrock,K.K., Sullivan,P., Martini-Stoica,H., Goldstein,D.S.,
            Graham,B.H., Yamamoto,S., Bellen,H.J., Gibbs,R.A., Chen,R.,
            D'Amelio,M. and Stoica,G.
  TITLE     Pleiotropic neuropathological and biochemical alterations
            associated with Myo5a mutation in a rat Model
  JOURNAL   Brain Res 1679, 155-170 (2018)
   PUBMED   29217155
  REMARK    GeneRIF: This study identified in vivo neuropathological and
            biochemical alterative changes involved in the pathogenesis of a
            spontaneous autosomal recessive movement disorder in a mutant Myo5a
            juvenile rat model.
REFERENCE   4  (bases 1 to 5608)
  AUTHORS   Guatteo,E., Rizzo,F.R., Federici,M., Cordella,A., Ledonne,A.,
            Latini,L., Nobili,A., Viscomi,M.T., Biamonte,F., Landrock,K.K.,
            Martini,A., Aversa,D., Schepisi,C., D'Amelio,M., Berretta,N. and
            Mercuri,N.B.
  TITLE     Functional alterations of the dopaminergic and glutamatergic
            systems in spontaneous alpha-synuclein overexpressing rats
  JOURNAL   Exp Neurol 287 (Pt 1), 21-33 (2017)
   PUBMED   27771352
REFERENCE   5  (bases 1 to 5608)
  AUTHORS   Ultanir,S.K., Yadav,S., Hertz,N.T., Oses-Prieto,J.A., Claxton,S.,
            Burlingame,A.L., Shokat,K.M., Jan,L.Y. and Jan,Y.N.
  TITLE     MST3 kinase phosphorylates TAO1/2 to enable Myosin Va function in
            promoting spine synapse development
  JOURNAL   Neuron 84 (5), 968-982 (2014)
   PUBMED   25456499
REFERENCE   6  (bases 1 to 5608)
  AUTHORS   Opartkiattikul,N., Funahara,Y., Fucharoen,S. and Talalak,P.
  TITLE     Increase in spontaneous platelet aggregation in
            beta-thalassemia/hemoglobin E disease: a consequence of splenectomy
  JOURNAL   Southeast Asian J Trop Med Public Health 23 Suppl 2, 36-41 (1992)
   PUBMED   1298991
REFERENCE   7  (bases 1 to 5608)
  AUTHORS   Mercer,J.A., Seperack,P.K., Strobel,M.C., Copeland,N.G. and
            Jenkins,N.A.
  TITLE     Novel myosin heavy chain encoded by murine dilute coat colour locus
  JOURNAL   Nature 349 (6311), 709-713 (1991)
   PUBMED   1996138
  REMARK    Erratum:[Nature 1991 Aug 8;352(6335):547]
REFERENCE   8  (bases 1 to 5608)
  AUTHORS   Moore,K.J., Swing,D.A., Copeland,N.G. and Jenkins,N.A.
  TITLE     Interaction of the murine dilute suppressor gene (dsu) with
            fourteen coat color mutations
  JOURNAL   Genetics 125 (2), 421-430 (1990)
   PUBMED   2379821
  REMARK    Erratum:[Genetics 1990 Sep;126(1):285]
REFERENCE   9  (bases 1 to 5608)
  AUTHORS   Moore,K.J., Seperack,P.K., Strobel,M.C., Swing,D.A., Copeland,N.G.
            and Jenkins,N.A.
  TITLE     Dilute suppressor dsu acts semidominantly to suppress the coat
            color phenotype of a deletion mutation, dl20J, of the murine dilute
            locus
  JOURNAL   Proc Natl Acad Sci U S A 85 (21), 8131-8135 (1988)
   PUBMED   3141922
REFERENCE   10 (bases 1 to 5608)
  AUTHORS   Murad,S. and Kishimoto,Y.
  TITLE     Alpha hydroxylation of lignoceric acid to cerebronic acid during
            brain development. Diminished hydroxylase activity in
            myelin-deficient mouse mutants
  JOURNAL   J Biol Chem 250 (15), 5841-5846 (1975)
   PUBMED   1150661
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Nov 27, 2020 this sequence version replaced NM_022178.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB035736.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-60                JAXUCZ010000008.1  84692949-84693008
            61-171              JAXUCZ010000008.1  84737211-84737321
            172-343             JAXUCZ010000008.1  84741640-84741811
            344-488             JAXUCZ010000008.1  84743618-84743762
            489-645             JAXUCZ010000008.1  84751759-84751915
            646-789             JAXUCZ010000008.1  84758095-84758238
            790-871             JAXUCZ010000008.1  84762533-84762614
            872-979             JAXUCZ010000008.1  84763372-84763479
            980-1086            JAXUCZ010000008.1  84766041-84766147
            1087-1352           JAXUCZ010000008.1  84768764-84769029
            1353-1434           JAXUCZ010000008.1  84770141-84770222
            1435-1575           JAXUCZ010000008.1  84773525-84773665
            1576-1701           JAXUCZ010000008.1  84775345-84775470
            1702-1785           JAXUCZ010000008.1  84777568-84777651
            1786-1947           JAXUCZ010000008.1  84781218-84781379
            1948-2045           JAXUCZ010000008.1  84782283-84782380
            2046-2132           JAXUCZ010000008.1  84784944-84785030
            2133-2241           JAXUCZ010000008.1  84785110-84785218
            2242-2453           JAXUCZ010000008.1  84790157-84790368
            2454-2610           JAXUCZ010000008.1  84791136-84791292
            2611-2850           JAXUCZ010000008.1  84793560-84793799
            2851-3099           JAXUCZ010000008.1  84795682-84795930
            3100-3193           JAXUCZ010000008.1  84798194-84798287
            3194-3342           JAXUCZ010000008.1  84802847-84802995
            3343-3456           JAXUCZ010000008.1  84805130-84805243
            3457-3600           JAXUCZ010000008.1  84811772-84811915
            3601-3654           JAXUCZ010000008.1  84812131-84812184
            3655-3882           JAXUCZ010000008.1  84813217-84813444
            3883-3891           JAXUCZ010000008.1  84814907-84814915
            3892-3992           JAXUCZ010000008.1  84816530-84816630
            3993-4191           JAXUCZ010000008.1  84820462-84820660
            4192-4285           JAXUCZ010000008.1  84824205-84824298
            4286-4432           JAXUCZ010000008.1  84838277-84838423
            4433-4587           JAXUCZ010000008.1  84840700-84840854
            4588-4677           JAXUCZ010000008.1  84846002-84846091
            4678-4828           JAXUCZ010000008.1  84847640-84847790
            4829-5111           JAXUCZ010000008.1  84849736-84850018
            5112-5286           JAXUCZ010000008.1  84852044-84852218
            5287-5367           JAXUCZ010000008.1  84854500-84854580
            5368-5608           JAXUCZ010000008.1  84854833-84855073
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q24"
     gene            1..5608
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /note="myosin VA"
                     /db_xref="GeneID:25017"
                     /db_xref="RGD:3143"
     exon            1..60
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     CDS             34..5520
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /note="isoform 2 is encoded by transcript variant 2;
                     dilute-opisthotonus; myosin-Va; dilute myosin heavy chain,
                     non-muscle; myosin 5a; unconventional myosin-Va"
                     /codon_start=1
                     /product="unconventional myosin-Va isoform 2"
                     /protein_id="NP_071514.2"
                     /db_xref="GeneID:25017"
                     /db_xref="RGD:3143"
                     /translation="MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEE
                     GKDLEYRLDPKTSELPHLRNPDILVGENDLTALSYLHEPAVLHNLRVRFIDSKLIYTY
                     CGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSII
                     VSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSS
                     RFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKM
                     LRLGNADSFHYTKQGGSPMIEGVDDAKEMAHTRQACTLLGISESYQMGIFRILAGILH
                     LGNVGFASRDSDSCTIPPKHEPLIIFCDLMGVDYEEMCHWLCHRKLATATETYIKPIS
                     KLQATNARDALAKHIYAKLFNWIVGHVNQALHSAVKQHSFIGVLDIYGFETFEINSFE
                     QFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIESKLGILDL
                     LDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRMSNKAFIIKHFADKVEYQCEGFLE
                     KNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRVPVKPTKGRP
                     GQTAKEHKKTVGLQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLR
                     ACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLGDRKQTCQNVLEKLILDKDK
                     YQFGKTKIFFRAGQVAYLEKLRADKLRAACIRIQKTIRGWLLRKRYLCMQRAAITVQR
                     YVRGYQARCYAKFLRRTKAATTIQKYWRMYVVRRKYKIRRAATIVLQSYLRGYLARNR
                     YRKILREHKAVIIQKRVRGWLARTHYKRTMKAIIYLQCCFRRMMAKRELKKLKIEARS
                     VERYKKLHIGMENKIMQLQRKVDEQNKDYKCLMEKLTNLEGVYNSETEKLRNDVERLQ
                     LSEEEAKVATGRVLSLQEEIAKLRKDLEQTRSEKKSIEERADKYKQETEQLVSNLKEE
                     NTLLKQEKETLNHLMVEQAKEMTETMERKLVEETKQLELDLNDERLRYQNLLNEFSRL
                     EERYDDLKEEMTLMLNVPKPGHKRTDSTHSSNESEYTFSSEFAETEDIAPRTEEPTEK
                     KVPLDMSLFLKLQKRVTELEQEKQLMQDELDRKEEQVLRSKAKEEERPQIRGAELEYE
                     SLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTAVSEELDVR
                     KEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNR
                     LLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEI
                     TRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRP
                     VNIPRKEKDFQGMLEYKREDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHAD
                     YLNDDQKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMK
                     HNTSRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQG
                     VSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYI
                     VGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQL
                     LQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQVRLRDR
                     KDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFIARV"
     exon            61..171
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            172..343
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            344..488
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            489..645
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            646..789
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            790..871
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            872..979
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1086
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1352
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1434
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            1435..1575
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1701
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            1702..1785
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..1947
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2045
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2132
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2241
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            2242..2453
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            2454..2610
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            2611..2850
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            2851..3099
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            3100..3193
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            3194..3342
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            3343..3456
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            3457..3600
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            3601..3654
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            3655..3882
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            3883..3891
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            3892..3992
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            3993..4191
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            4192..4285
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            4286..4432
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            4433..4587
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            4588..4677
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            4678..4828
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            4829..5111
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            5112..5286
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            5287..5367
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
     exon            5368..5608
                     /gene="Myo5a"
                     /gene_synonym="Dop; Myh12"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcccaggcgg ggggcgtcgg cgctgggccc gccatggccg cgtccgagct ctacaccaag
       61 tttgcccggg tttggatacc tgatcctgag gaagtgtgga aatcagctga gttgctcaag
      121 gactataaac ctggagataa ggtgctcctg cttcaccttg aggaagggaa ggatttggaa
      181 taccgtctag atccaaagac cagcgagctc cctcacctac ggaaccctga catacttgtt
      241 ggagaaaatg acctcacagc cctcagctac cttcacgagc ctgctgtgct gcacaatctc
      301 cgcgtccgct tcatcgactc caaactcatt tatacgtatt gtgggatagt cctggtagct
      361 ataaatccct atgagcagct gcctatctat ggagaagata tcattaatgc atacagtggc
      421 cagaacatgg gtgacatgga ccctcacatc tttgcagtag ctgaagaggc ctataaacaa
      481 atggcaaggg acgaacgaaa tcagtccatc attgtaagtg gagagtcagg tgcagggaag
      541 acagtctcgg ctaagtatgc catgaggtac ttcgcaactg taagtggctc tgccagtgag
      601 gccaatgttg aggaaaaggt cttggcctcc aaccccatca tggagtcaat tggaaatgct
      661 aaaacaacca ggaatgataa tagcagccgg tttgggaaat atattgaaat tggttttgac
      721 aagagatatc gaatcatcgg tgccaatatg agaacttacc ttttagagaa atccagagtg
      781 gtgttccagg cagaagagga gagaaactac catatcttct atcagctctg tgcttcggca
      841 aagctacctg agtttaagat gctgcggtta ggaaatgcag atagtttcca ttacacgaag
      901 caaggaggca gccctatgat agaaggagtg gacgatgcga aggagatggc acacaccagg
      961 caggcctgca ctctgctggg aattagtgaa tcttaccaaa tgggaatttt tcggatactt
     1021 gctggcattc ttcacttagg caatgttggg tttgcgtctc gagattcaga cagctgcaca
     1081 atacctccca agcacgagcc tcttatcatt ttctgtgatc ttatgggtgt ggattatgaa
     1141 gagatgtgtc actggctctg ccaccgaaag ctagctaccg ccacagagac gtacatcaag
     1201 cccatctcca agctgcaggc cacaaatgcc cgagacgctt tagcaaagca tatctatgca
     1261 aagctcttta actggattgt cggccatgtc aatcaggctc tccattcagc tgtcaagcag
     1321 cactctttca tcggcgtgct ggacatttat ggatttgaaa catttgagat aaatagtttt
     1381 gaacagttct gcataaatta tgcaaatgaa aagctacagc aacaattcaa catgcatgtc
     1441 ttcaaactgg aacaggagga gtacatgaag gaacaaattc catggacact catagatttc
     1501 tatgataatc agccttgtat caatcttata gaatctaaac tgggtattct agatttgctg
     1561 gatgaggaat gtaagatgcc taaaggtacc gatgacacat gggcccaaaa actatacaac
     1621 acacatttga acaaatgtgc tctctttgag aagccccgca tgtcaaacaa agctttcatc
     1681 atcaaacatt ttgctgacaa agtggagtac cagtgtgaag gttttcttga aaagaataaa
     1741 gacactgttt ttgaagaaca aattaaagtc cttaagtcaa gcaagtttaa gatgctacca
     1801 gaactatttc aagatgacga gaaggccatc agtcccacct ctgccacttc ctcaggacgc
     1861 acacctctca cacgggtacc tgtaaagccc accaagggtc gacctggcca gactgccaaa
     1921 gagcacaaga agacagtggg acttcagttt cgaaactccc ttcacctgct tatggaaacc
     1981 cttaacgcca ctactcctca ctatgttcgc tgtattaagc ccaatgattt caagtttcca
     2041 ttcacatttg atgagaagag agcagtacag cagctaagag catgtggtgt cctggagacc
     2101 atccggatca gcgcagcagg gtttccctca aggtggactt atcaagagtt tttcagccgg
     2161 taccgggtcc taatgaagca aaaggatgtg ctgggagata gaaagcaaac atgccagaat
     2221 gtattagaga aactgatact ggacaaggat aaataccagt ttggtaagac aaagatcttt
     2281 tttcgtgctg gtcaagtggc ctatctagaa aaattgaggg ctgacaaact gagggcggcc
     2341 tgcatccgga tccagaagac gattcgtggg tggctgctga ggaagagata cctgtgtatg
     2401 cagagggcag ccatcacagt gcagcgatac gtgcgtggct atcaggctcg atgctatgct
     2461 aagtttctgc gcagaaccaa ggcagcaacc accattcaga agtactggcg catgtatgtg
     2521 gtccgcagga agtacaagat tagacgagct gccaccattg ttcttcagtc ttatttgaga
     2581 ggctacttgg caagaaatag gtatcgcaag atactccgtg agcacaaagc agtcatcatt
     2641 cagaaacgtg tccgtggctg gctggctcgt acacattata agaggaccat gaaagccatc
     2701 atctaccttc aatgctgctt ccggcggatg atggccaagc gtgagctgaa gaaactcaaa
     2761 attgaggctc gctctgtgga acgctacaag aagctccata ttggcatgga aaacaagatt
     2821 atgcagctgc aacgcaaagt ggatgagcag aataaagact acaaatgcct catggagaaa
     2881 ctgaccaatc tagaaggagt atacaactct gagactgaaa aactacgaaa tgatgtagaa
     2941 cgtcttcagc taagcgagga ggaagctaag gttgccactg ggagagtgct tagtctgcag
     3001 gaagaaattg caaaactccg aaaagacctg gaacaaactc gatcggagaa aaagtccatt
     3061 gaagaacgag cagataaata caaacaagaa actgagcagc tggtgtcaaa cttgaaggaa
     3121 gaaaacactc tgctgaagca ggaaaaggag accctcaatc acctcatggt ggagcaggct
     3181 aaggagatga cagaaactat ggagaggaag ttagtagaag aaacaaaaca actggagctc
     3241 gacctgaatg atgagaggct gaggtatcag aacctcctga atgagttcag tcgtctggag
     3301 gagcgctatg acgacctcaa ggaagagatg accctgatgc tgaatgtgcc taagccagga
     3361 cacaagagaa cagactctac ccacagcagc aatgagtctg aatacacctt cagctcagaa
     3421 tttgcagaaa ctgaagacat tgcaccaagg acagaggagc caactgagaa gaaggtgcct
     3481 ctggatatgt cattgttcct taagctccag aagcgtgtca cagagctgga acaggagaag
     3541 cagttgatgc aggatgagct ggaccgaaag gaggagcagg tgttgcgcag caaggcaaag
     3601 gaagaagaaa gaccacagat tagaggtgct gaactggaat atgagtccct caagcgtcag
     3661 gaactggagt cagaaaacaa aaagctgaag aatgagctga atgagctacg caaagccctg
     3721 agtgagaaga gtgcccccga agtgactgcg ccaggcgcgc ccgcttaccg cgtcctcatg
     3781 gagcagctga cggccgtgag cgaggagctc gatgtccgca aggaggaagt cctcatctta
     3841 aggtctcagc tggtgagcca gaaagaagcc atccaaccca aggatgacaa gaatacaatg
     3901 acagattcca caatactttt ggaagatgta cagaaaatga aagacaaagg tgaaatagca
     3961 caagcatata ttggtttgaa agaaacgaac aggctcttag aatcccagct acagtcgcag
     4021 aagaggagcc atgaaaatga ggctgaggcc ctccgtgggg agatccagag cctaaaggaa
     4081 gaaaacaacc ggcaacagca gctgctggcc cagaacctgc agctgccccc tgaggcccgc
     4141 attgaggcca gcctgcagca tgagatcacc cggctgacca atgaaaacct ggatctgatg
     4201 gaacaacttg aaaagcagga taaaaccgtc cggaaactga agaaacaact aaaagtcttt
     4261 gccaaaaaaa tcggtgaact agaagtgggg cagatggaga acatatcccc aggacagatc
     4321 atcgatgagc ctatccggcc ggtcaacatt ccccggaaag aaaaggattt ccaagggatg
     4381 ctggagtaca agcgggagga cgaacagaag cttgttaaga acctgattct agaactaaag
     4441 ccacgtggtg tggctgtcaa tctgattcca gggttaccgg catatatctt gtttatgtgt
     4501 gtgcgacatg ccgactacct gaacgatgat cagaaagtaa gatcattgct gacatcaaca
     4561 attaacagca tcaaaaaagt cctgaagaaa agaggtgacg attttgaaac tgtctccttc
     4621 tggctctcta acacatgtcg atttttgcac tgtttgaagc aatatagtgg agaagagggc
     4681 ttcatgaaac acaacacgtc tcgccagaat gaacactgcc tcaccaattt tgaccttgct
     4741 gagtatcggc aagtactgag tgacttggcc attcagatct atcagcagct tgtgagggtg
     4801 ttagagaaca ttcttcagcc aatgatagtc tcaggcatgc tagagcatga aacaattcag
     4861 ggagtatctg gggtgaagcc cacaggactc agaaagcgaa cctccagtat cgccgatgag
     4921 ggcacctaca cactggactc catcctgcgg cagctcaact ccttccattc cgtcatgtgt
     4981 cagcatggca tggacccaga gctaatcaag caggtggtca agcagatgtt ctacatcgtg
     5041 ggcgccatca ccctgaacaa cctcctgctg cgcaaggaca tgtgctcctg gagcaaaggc
     5101 atgcagatac ggtacaatgt cagtcaattg gaagaatggc tacgtgacaa gaatctaatg
     5161 aacagtgggg caaaagagac tctggaacct cttatccagg ctgctcagct tttgcaagtg
     5221 aaaaagaaaa ctgacgatga cgcggaagcc atctgctcca tgtgcaacgc tctgaccaca
     5281 gcccagatcg tcaaagtgtt gaatctgtac acacccgtta atgagtttga ggaaagggtc
     5341 tctgtttcat ttatccgcac tatacaggtg cggttacgag acaggaaaga ctctccacag
     5401 ctgctcatgg atgcaaaaca catctttcct gtcactttcc cctttaaccc atcctccctg
     5461 gccctagaaa ccatccagat tccagccagc ctgggcctgg gcttcattgc aagggtctga
     5521 agggctaccc aagcaaatgt agagaacgca tttctcctca aaacatgaac agttgtttcc
     5581 agtgagctac tgaaaataca tttttaaa
//
