LOCUS       NM_001110810            2732 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Rattus norvegicus myosin light chain kinase 3 (Mylk3), mRNA.
ACCESSION   NM_001110810 XM_001064628 XM_214645
VERSION     NM_001110810.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Avila-Rodriguez,D., Solano Agama,C., Gonzalez-Pozos,S., Vicente
            Mendez-Mendez,J., Ortiz Plata,A., Arreola-Mendoza,L. and
            Mendoza-Garrido,M.E.
  TITLE     The shift in GH3 cell shape and cell motility is dependent on MLCK
            and ROCK
  JOURNAL   Exp Cell Res 354 (1), 1-17 (2017)
   PUBMED   28300565
  REMARK    GeneRIF: We found that a rounded shape with high cortical tension
            depends on MLCK and ROCK, whereas in cells with an elongated shape,
            MLCK is the primary protein responsible for cell spreading.
            Further, in cells with a slow and directionally persistent
            motility, MLCK predominates, while rapid and erratic movement is
            ROCK-dependent
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Eikemo,H., Moltzau,L.R., Hussain,R.I., Nguyen,C.H., Qvigstad,E.,
            Levy,F.O., Skomedal,T. and Osnes,J.B.
  TITLE     CaMKII in addition to MLCK contributes to phosphorylation of
            regulatory light chain in cardiomyocytes
  JOURNAL   Biochem Biophys Res Commun 471 (1), 219-225 (2016)
   PUBMED   26809094
  REMARK    GeneRIF: In conclusion, in addition to MLCK, CaMKII phosphorylates
            RLC in cardiomyocytes.
REFERENCE   3  (bases 1 to 2732)
  AUTHORS   Gao,L., Zheng,Y.J., Gu,S.S., Tan,J.L., Paul,C., Wang,Y.G. and
            Yang,H.T.
  TITLE     Degradation of cardiac myosin light chain kinase by matrix
            metalloproteinase-2 contributes to myocardial contractile
            dysfunction during ischemia/reperfusion
  JOURNAL   J Mol Cell Cardiol 77, 102-112 (2014)
   PUBMED   25451385
  REMARK    GeneRIF: Ischemia/reperfusion-activated MMP-2 leads to the
            degradation of cardiac MLCK.
REFERENCE   4  (bases 1 to 2732)
  AUTHORS   Al-Sadi,R., Ye,D., Dokladny,K. and Ma,T.Y.
  TITLE     Mechanism of IL-1beta-induced increase in intestinal epithelial
            tight junction permeability
  JOURNAL   J Immunol 180 (8), 5653-5661 (2008)
   PUBMED   18390750
REFERENCE   5  (bases 1 to 2732)
  AUTHORS   Chan,J.Y., Takeda,M., Briggs,L.E., Graham,M.L., Lu,J.T.,
            Horikoshi,N., Weinberg,E.O., Aoki,H., Sato,N., Chien,K.R. and
            Kasahara,H.
  TITLE     Identification of cardiac-specific myosin light chain kinase
  JOURNAL   Circ Res 102 (5), 571-580 (2008)
   PUBMED   18202317
REFERENCE   6  (bases 1 to 2732)
  AUTHORS   Seguchi,O., Takashima,S., Yamazaki,S., Asakura,M., Asano,Y.,
            Shintani,Y., Wakeno,M., Minamino,T., Kondo,H., Furukawa,H.,
            Nakamaru,K., Naito,A., Takahashi,T., Ohtsuka,T., Kawakami,K.,
            Isomura,T., Kitamura,S., Tomoike,H., Mochizuki,N. and Kitakaze,M.
  TITLE     A cardiac myosin light chain kinase regulates sarcomere assembly in
            the vertebrate heart
  JOURNAL   J Clin Invest 117 (10), 2812-2824 (2007)
   PUBMED   17885681
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from EU236721.1.
            
            On or before Nov 24, 2007 this sequence version replaced
            XM_001064628.1, XM_214645.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU236721.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760434, SAMN06621351
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q11"
     gene            1..2732
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /note="myosin light chain kinase 3"
                     /db_xref="GeneID:291926"
                     /db_xref="RGD:1305801"
     exon            1..723
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     CDS             238..2598
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /EC_number="2.7.11.18"
                     /function="phosphorylates MLC-2v"
                     /note="cardiac-MLCK; cardiac-MyBP-C-associated Ca/CaM
                     kinase; putative myosin light chain kinase 3"
                     /codon_start=1
                     /product="myosin light chain kinase 3"
                     /protein_id="NP_001104280.1"
                     /db_xref="GeneID:291926"
                     /db_xref="RGD:1305801"
                     /translation="MSGVSEEDPEGLGPQGLPALGGACLVTVDKKLNVLTEKVDRLLH
                     FQEDVTEKLQCVCQGMDHLEQGLHRLEASQELGLAGPGSTSPAAAQAAWPEVLELVRA
                     VRQEGAQHGARLEALFKMVVAVDRAITLVGSTIQNSKVDDFILQGTVPWRKGSLADGP
                     EENKEQAEVAGVKPKHVLNTGSVQAATSRALWEESQKQDTPVGTVEGLPLIIDTSLKG
                     ADLTQAGASLRQGVEALDPGQEPPPAEAESRLPALASEDTGTTLELSVAIDRISEVLT
                     SLRMSQSAGEGTSSSKPDCSEPGPQPLGPLTTDSDIHSDEGLPRISVRMREMTTPEEL
                     FETQGGSPIGSAEAPGPGTVLEDQIPKGARPFPPLPKRSCNNGGASAEEATGPGAEPI
                     RGPSLVTRDWRDEPVGTTDLQQGRDPGAVSPEPGKDHAAQGPGRTEAGRRVSSAAEAA
                     IVVLDDSAAPPAPFEHRVVSIKDTLISTSYTVSQHEVLGGGRFGQVHRCTERSTGLAL
                     AAKIIKVKNIKDREDVKNEINIMNQLSHVNLIQLYDAFESKNSFTLIMEYVDGGELFD
                     RITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQTGHQIKIIDF
                     GLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGE
                     TDAETMNFIVNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLNHL
                     IAKASGSNVRLRSQLLLQKYMAQRKWKKHFHVVTAVNRLRKFPTCP"
     exon            724..817
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            818..1196
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1600
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1706
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1800
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1910
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..2052
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2123
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            2124..2252
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2405
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            2406..2538
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
     exon            2539..2732
                     /gene="Mylk3"
                     /gene_synonym="MLCK; RGD1305801"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 caagggcctg ggccaaactc agcctgtcgc tctctcagtg gttgagtggg agatagacac
       61 agagaacttg gccctctccg tcccccacac agttcagtca ggaggacagg ttggttccct
      121 gcagcaaggc tcttagcagc ggaggacaat ggccttccag acgttaccac caaagtgaga
      181 agcagaccct tcgtgctcca gtttccttgt ctttgccctt accaaaccag aattgggatg
      241 tcaggagttt cagaggagga tccagagggt ctgggccccc agggtctgcc agcgttgggc
      301 ggagcctgct tagtcaccgt ggacaaaaaa cttaatgtgc tgactgagaa ggtcgacaga
      361 ctcttgcatt tccaagaaga tgtcacagag aagctacagt gtgtgtgcca aggcatggat
      421 cacctggaac aaggtctgca tcggctggag gcctcccagg agttgggtct ggcagggccc
      481 ggcagcactt ccccagccgc tgctcaggcc gcatggcctg aggtcctgga gctggtgagg
      541 gccgtgcggc aggagggtgc ccagcacggt gccaggctcg aagccctctt caagatggtg
      601 gtggctgtgg acagggctat tactttggta gggtccacaa tccagaactc caaagtggat
      661 gatttcatcc tgcaagggac cgtgccctgg aggaaaggca gtctggctga tggccctgag
      721 gagaacaagg agcaagcaga agtggctgga gtgaagccaa agcatgtgct gaatacagga
      781 agtgtgcaag ctgccacttc tagggcgctg tgggaagaga gccagaagca ggacacaccc
      841 gtggggacag tggaggggct gcctctcatc atcgatacgt cactgaaggg agctgacctc
      901 acccaggctg gagcctcact gaggcaggga gttgaagctc ttgacccagg ccaagaaccc
      961 ccacccgcag aggcagaatc caggcttcct gcactagcca gcgaggacac tgggaccacc
     1021 ctggaattgt ctgtagcaat tgacagaatc agtgaggtcc tcactagcct caggatgtcc
     1081 caaagtgctg gcgaaggaac ctcatccagc aagcctgact gttcagagcc tggccctcag
     1141 cccctagggc cactaactac agacagtgac attcacagtg atgaaggact tcccaggatc
     1201 tctgtccgta tgcgagagat gactactcct gaggagctgt ttgagaccca aggtggcagc
     1261 cccattggct cggcagaagc tccaggccct ggaactgtgt tagaagacca gatccctaaa
     1321 ggagccagac catttccacc cctgccaaag aggagctgca acaatggtgg cgcgagtgca
     1381 gaggaggcaa cagggcctgg ggctgagccc atcagaggac caagcttggt cacaagggac
     1441 tggagagatg aacctgttgg gaccacagac ctgcagcaag gcagagaccc aggagcggtg
     1501 agccctgaac ctgggaagga ccatgcagcc cagggcccag ggagaaccga agctggaagg
     1561 agggtgtctt ctgctgcaga ggctgccatc gtagttctag atgacagcgc agcaccccca
     1621 gccccttttg aacaccgggt agtgagcatc aaagacaccc tcatctcgac aagttacaca
     1681 gtgtcccaac acgaagtctt gggagggggc cggtttggcc aggtgcacag gtgtacagag
     1741 cggtccacag gccttgcact ggcagccaag atcatcaaag tgaagaacat aaaggaccgg
     1801 gaggatgtga agaatgagat caacatcatg aaccagctca gccacgtaaa cttgatccaa
     1861 ctttatgatg cttttgagag caagaacagc ttcaccctga tcatggagta tgtggatgga
     1921 ggtgaactct tcgaccggat cacggatgag aagtaccacc tgaccgagct ggatgtggtc
     1981 ttgttcacaa ggcagatctg tgagggtgtg cattacctgc accagcacta cattctgcac
     2041 ctggacctca agccagagaa catactgtgt gtcagccaga ctgggcatca aattaagatt
     2101 attgactttg ggctggctag aagatataaa cctcgggaga agctaaaggt taactttggt
     2161 actccagagt tcctggctcc agaagttgtt aactatgagt ttgtctcatt cccaacagac
     2221 atgtggagtg tgggagttat cacctacatg ctactcagtg gtttgtcccc atttctaggg
     2281 gaaacagatg cagagaccat gaattttatt gtgaactgca gctgggattt cgatgctgat
     2341 accttcaaag ggctgtcaga ggaagccaag gactttgttt cacggttgct ggtcaaagag
     2401 aagagttgta ggatgagcgc cacacagtgc ctgaaacatg agtggttaaa tcacctgatt
     2461 gccaaagcct caggctccaa cgttcgcctc agatcccaac tactgctgca gaaatatatg
     2521 gctcagcgta aatggaagaa acatttccat gtggtgactg cagtcaacag gctaagaaaa
     2581 tttccaacgt gtccctaatc tacaactggg cctgggagtt cctgaggcga cacgcagtgg
     2641 taatgtgaag agatgactca ggattttatg gagtcaggag cttggctgtt attgatctta
     2701 ttttgcaaag aatggtggaa ggaagaaaga ga
//
