LOCUS       NM_057209               2816 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus myosin light chain kinase 2 (Mylk2), mRNA.
ACCESSION   NM_057209
VERSION     NM_057209.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Hu,S., Cheng,M., Guo,X., Wang,S., Liu,B., Jiang,H., Huang,C. and
            Wu,G.
  TITLE     Down-regulation of miR-200c attenuates AngII-induced cardiac
            hypertrophy via targeting the MLCK-mediated pathway
  JOURNAL   J Cell Mol Med 23 (4), 2505-2516 (2019)
   PUBMED   30680929
  REMARK    GeneRIF: Down-regulation of miR-200c attenuates AngII-induced
            cardiac hypertrophy via targeting the MLCK-mediated pathway.
            Erratum:[J Cell Mol Med. 2023 Jul;27(14):2093-2094. doi:
            10.1111/jcmm.17724.. PMID: 37395337]
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Leitman,E.M., Tewari,A., Horn,M., Urbanski,M., Damanakis,E.,
            Einheber,S., Salzer,J.L., de Lanerolle,P. and Melendez-Vasquez,C.V.
  TITLE     MLCK regulates Schwann cell cytoskeletal organization,
            differentiation and myelination
  JOURNAL   J Cell Sci 124 (Pt 22), 3784-3796 (2011)
   PUBMED   22100921
  REMARK    GeneRIF: MLCK regulates Schwann cell cytoskeletal organization,
            cell differentiation and myelination.
REFERENCE   3  (bases 1 to 2816)
  AUTHORS   Al Madhoun,A.S., Mehta,V., Li,G., Figeys,D., Wiper-Bergeron,N. and
            Skerjanc,I.S.
  TITLE     Skeletal myosin light chain kinase regulates skeletal myogenesis by
            phosphorylation of MEF2C
  JOURNAL   EMBO J 30 (12), 2477-2489 (2011)
   PUBMED   21556048
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2816)
  AUTHORS   Barfod,E.T., Moore,A.L., Van de Graaf,B.G. and Lidofsky,S.D.
  TITLE     Myosin light chain kinase and Src control membrane dynamics in
            volume recovery from cell swelling
  JOURNAL   Mol Biol Cell 22 (5), 634-650 (2011)
   PUBMED   21209319
  REMARK    GeneRIF: Src and MLCK form a swelling-induced protein complex that
            regulates volume recovery through membrane turnover and
            compensatory endocytosis under osmotic stress.
REFERENCE   5  (bases 1 to 2816)
  AUTHORS   Tinsley,J.H., Hunter,F.A. and Childs,E.W.
  TITLE     PKC and MLCK-dependent, cytokine-induced rat coronary endothelial
            dysfunction
  JOURNAL   J Surg Res 152 (1), 76-83 (2009)
   PUBMED   18621396
  REMARK    GeneRIF: PKC and MLCK pathways important in coronary endothelial
            dysfunction elicited by ischemia/reperfusion injury and cytokine
            release
REFERENCE   6  (bases 1 to 2816)
  AUTHORS   Bozzo,C., Stevens,L., Toniolo,L., Mounier,Y. and Reggiani,C.
  TITLE     Increased phosphorylation of myosin light chain associated with
            slow-to-fast transition in rat soleus
  JOURNAL   Am J Physiol Cell Physiol 285 (3), C575-C583 (2003)
   PUBMED   12748068
  REMARK    GeneRIF: in rat skeletal muscles an increase of MLC2
            phosphorylation is associated with the slow-to-fast transition
            regardless of whether hypertrophy or atrophy develops.
REFERENCE   7  (bases 1 to 2816)
  AUTHORS   Chen,B.H., Tzen,J.T., Bresnick,A.R. and Chen,H.C.
  TITLE     Roles of Rho-associated kinase and myosin light chain kinase in
            morphological and migratory defects of focal adhesion kinase-null
            cells
  JOURNAL   J Biol Chem 277 (37), 33857-33863 (2002)
   PUBMED   12105199
  REMARK    GeneRIF: Rho-kinase and MLC kinase play a role in migratory defect
            in FAK null cells.
REFERENCE   8  (bases 1 to 2816)
  AUTHORS   Liu,X., Takeda,N. and Dhalla,N.S.
  TITLE     Myosin light-chain phosphorylation in diabetic cardiomyopathy in
            rats
  JOURNAL   Metabolism 46 (1), 71-75 (1997)
   PUBMED   9005973
REFERENCE   9  (bases 1 to 2816)
  AUTHORS   Herring,B.P., Nunnally,M.H., Gallagher,P.J. and Stull,J.T.
  TITLE     Molecular characterization of rat skeletal muscle myosin light
            chain kinase
  JOURNAL   Am J Physiol 256 (2 Pt 1), C399-C404 (1989)
   PUBMED   2465691
REFERENCE   10 (bases 1 to 2816)
  AUTHORS   Roush,C.L., Kennelly,P.J., Glaccum,M.B., Helfman,D.M., Scott,J.D.
            and Krebs,E.G.
  TITLE     Isolation of the cDNA encoding rat skeletal muscle myosin light
            chain kinase. Sequence and tissue distribution
  JOURNAL   J Biol Chem 263 (21), 10510-10516 (1988)
   PUBMED   2839493
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Dec 7, 2020 this sequence version replaced NM_057209.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643295.15825.1,
                                           SRR26643296.11681.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                JAXUCZ010000003.1  161836767-161836838
            73-172              JAXUCZ010000003.1  161836942-161837041
            173-644             JAXUCZ010000003.1  161837509-161837980
            645-934             JAXUCZ010000003.1  161838635-161838924
            935-1040            JAXUCZ010000003.1  161840301-161840406
            1041-1134           JAXUCZ010000003.1  161840804-161840897
            1135-1244           JAXUCZ010000003.1  161843011-161843120
            1245-1386           JAXUCZ010000003.1  161843203-161843344
            1387-1457           JAXUCZ010000003.1  161845021-161845091
            1458-1586           JAXUCZ010000003.1  161845196-161845324
            1587-1739           JAXUCZ010000003.1  161845888-161846040
            1740-1872           JAXUCZ010000003.1  161846147-161846279
            1873-2816           JAXUCZ010000003.1  161847666-161848609
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q41"
     gene            1..2816
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /note="myosin light chain kinase 2"
                     /db_xref="GeneID:117558"
                     /db_xref="RGD:620934"
     exon            1..72
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /inference="alignment:Splign:2.1.0"
     exon            73..172
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..1953
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /EC_number="2.7.11.18"
                     /note="MLCK2; myosin light chain kinase 2, skeletal
                     muscle; myosin, light polypeptide kinase 2"
                     /codon_start=1
                     /product="myosin light chain kinase 2, skeletal/cardiac
                     muscle"
                     /protein_id="NP_476557.2"
                     /db_xref="GeneID:117558"
                     /db_xref="RGD:620934"
                     /translation="MATENGAVELGTQSLSTEQTPKDAAGDGSPASEKEPSLPDTEKD
                     LGPTNTKKDPGAPDPKKNPDPPSLKKTPEAPGPEKKGDSAPASASNQGPSGEGDGGGG
                     PAEGGTGPPAVLPQPTATADASIQKLDATQAPSGNQESGEAKAGKKAAECREAGRRGS
                     PAFLHSPSCPAIISCSEKTLAMKPLSETTELIFAGVSETPDPQDPGPAKAEGGTNTLA
                     ERKEEAEAGQAEQAKVQGDTSQRIGFQAVPSERAEVGQALCLTAKEEDCFQILDDCPP
                     PPAPFPHRIVELRTGNVNSEFSMNSKEALGGGKFGAVCTCTERSTGLKLAAKVIKKQT
                     PKDKEMVLLEIEVMNQLNHRNLIQLYSAIETSHEIILFMEYIEGGELFERIVDEDYQL
                     TEVDTMVFVRQICDGILFMHKMRVLHLDLKPENILCVNTTGHLVKIIDFGLARRYNPN
                     EKLKVNFGTPEFLSPEVVNYDQISDKTDMWSLGVITYMLLSGLSPFLGDDDTETLNNV
                     LSANWYFDEETFEAVSDEAKDFVSNLITKDQSARMSAEQCLAHPWLNNLAEKAKRCNR
                     RLKSQILLKKYLMKRRWKKNFIAVSAANRFKKISSSGALMALGV"
     exon            173..644
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /inference="alignment:Splign:2.1.0"
     exon            645..934
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1040
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1134
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1244
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1386
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1457
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1586
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /inference="alignment:Splign:2.1.0"
     exon            1587..1739
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /inference="alignment:Splign:2.1.0"
     exon            1740..1872
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..2816
                     /gene="Mylk2"
                     /gene_synonym="Klmc; Mlck"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acaaccaccc caggatttcg cctgactagc tgcagcagac tgccggccac caggttgccc
       61 ctctccattc agctagaaag acttgagtta gacaagcaga agcacacgcc tacctacctc
      121 atggctacag aaaacggagc agttgagctg ggaacccaga gtctgtcaac agaacaaaca
      181 cctaaagacg cagcaggtga tggatctcca gcttcagaga aagaacccag cctcccagac
      241 acagagaaag acctgggccc cactaacacg aagaaagacc ctggcgcgcc ggatccaaag
      301 aaaaatccag atccaccctc cctgaagaaa actcccgaag cccctggtcc agagaaaaag
      361 ggtgactcgg ccccagcttc agccagcaac cagggccctt caggagaggg tgatggaggt
      421 gggggccctg ctgaaggcgg tacggggcct cctgcagtct tgccccagcc cactgccaca
      481 gctgatgcca gcatccagaa gctggacgcc acgcaagcgc cctcgggcaa ccaggaatct
      541 ggagaggcca aggcaggcaa gaaggcagca gaatgccgag aagcaggacg aaggggctca
      601 cccgcctttc ttcacagccc tagctgcccc gcgatcatct cctgttctga gaagacactg
      661 gccatgaagc ccttaagcga gacaacagag ctcatctttg caggggtgtc tgaaacccct
      721 gatccccaag atcctggtcc agctaaggca gaaggaggga cgaacacgct ggcagagcgg
      781 aaagaagagg cagaggcagg ccaggctgag caggctaagg tgcaagggga cacctcccag
      841 aggatcgggt tccaggctgt tccctcagag agagcagagg tggggcaggc cctctgtctc
      901 acggccaagg aggaagactg cttccaaatt ctggatgatt gtccgccacc cccagccccc
      961 ttcccacaca ggatcgtgga gctgaggacc ggaaatgtca acagtgaatt cagcatgaac
     1021 tcaaaggagg cattgggagg tggcaagttt ggagctgtgt gcacctgcac ggagagatcc
     1081 acaggcctca agctggcagc caaggtcatc aagaaacaga cccccaagga caaggaaatg
     1141 gtgttgctgg agatcgaggt catgaaccag ctgaaccacc gcaatttgat ccagctgtac
     1201 tcagccattg agacctctca cgagatcatc ctgttcatgg agtacattga gggcggcgag
     1261 ctctttgaga ggatcgtgga tgaggactac caactgacgg aagtggatac catggtgttc
     1321 gtcaggcaga tctgcgacgg gatcctcttc atgcacaaga tgagggtcct gcacttggac
     1381 ctcaagccag agaacatcct gtgtgtgaat accaccgggc acttggtgaa gataattgac
     1441 ttcggcctgg caaggaggta taaccccaat gagaagctga aggtgaattt tgggaccccg
     1501 gagttcctgt caccagaggt ggtgaactat gaccagatct ctgacaagac ggacatgtgg
     1561 agtcttgggg tgatcaccta catgctactg agtggcctct ctcccttcct gggcgacgat
     1621 gacacggaga ccctaaacaa cgtcctgtct gccaactggt actttgatga ggagactttt
     1681 gaagctgtgt cagacgaggc caaagacttc gtttccaatc ttatcacgaa ggaccagagt
     1741 gccagaatga gtgctgagca gtgcctcgcc cacccctggc tcaacaacct ggcagaaaaa
     1801 gccaagcgct gcaaccgtcg tctcaagtcc cagatcctgc ttaagaaata cctcatgaag
     1861 aggcgctgga agaaaaactt cattgctgtc agcgcagcca atcgcttcaa gaagatcagt
     1921 agctcggggg cactgatggc tttgggggtc tgaaccccag gagcggctaa agcctggatg
     1981 cagctgcgca gtggtcaagg ctgaaaccac acagctcaga aggccagaga gagccggcca
     2041 gatctctaga gcaggctggt caggacaaag ctgcaccagg cccagaggct tggggctccg
     2101 caggtcctta ggcttaccgc ttcccctacg tgaacatgga gtgtggcctg gaatccatct
     2161 tgctaccgcc tcctcagcag ggctgtggcc tgtcagcccc acccagactc caaggacagc
     2221 caggccattc cctagtctct ccctagcttg cccctttgaa ctgctgcccc gtagcctttt
     2281 ttttgcccca cctggggcac tcttgcctgg agcgggatgg ccgggggtcc agcaggtggg
     2341 ctgctgtagt ggtggtggtg gatgggaaga ttctaggagg gtaagaatgc agtccagctg
     2401 ccaggggaga cagagcaggc tttgtgtgag aggacctcca tgccctgcct cacactccca
     2461 acagatacag ccttgcacac accatctggc ctcctggctc cccaccttct gtttttgtga
     2521 ccaccaacac ataggaactt tgtgagagaa agccagccca ggcctggaga ggtggtggtg
     2581 gtagcggaga gaagtctggg aggcctgaga ccacccccct aagtttactt gaggaccgag
     2641 tcttcccatc ctggctactc tgtacccatc ccaggggcat ctgaaacccc aaataacagg
     2701 atgaactggc cacaaggagt atgaagccgt cgggcagacc ccaacctgct ctcagcttgt
     2761 gccttataaa taaacacaca ggttggaggc agcctccttc cctctgcgca tgcaca
//
