LOCUS       NM_001105874            7764 bp    mRNA    linear   ROD 26-APR-2024
DEFINITION  Rattus norvegicus myosin light chain kinase (Mylk), mRNA.
ACCESSION   NM_001105874 XM_001071705 XM_213611
VERSION     NM_001105874.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Wang,S., Wang,R., Li,G.Q., Cho,J.L., Deng,Y. and Li,Y.
  TITLE     Myosin light chain kinase mediates intestinal barrier dysfunction
            following simulated microgravity based on proteomic strategy
  JOURNAL   J Proteomics 231, 104001 (2021)
   PUBMED   33035716
  REMARK    GeneRIF: Myosin light chain kinase mediates intestinal barrier
            dysfunction following simulated microgravity based on proteomic
            strategy.
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   Wang,S., Cheng,M., Hu,Z., Hu,S., Zou,Q., Lai,X., Liu,B., Jiang,H.,
            Huang,C. and Wu,G.
  TITLE     Angiotensin II Facilitates Matrix Metalloproteinase-9-Mediated
            Myosin Light Chain Kinase Degradation in Pressure Overload-Induced
            Cardiac Hypertrophy
  JOURNAL   Cell Physiol Biochem 44 (6), 2281-2295 (2017)
   PUBMED   29262413
  REMARK    GeneRIF: Ang II is involved in the degradation of myosin light
            chain kinase in pressure overload-induced cardiac hypertrophy and
            that this process was mediated by MMP9.
REFERENCE   3  (bases 1 to 7764)
  AUTHORS   Li,L., Wu,X., Yue,H.Y., Zhu,Y.C. and Xu,J.
  TITLE     Myosin light chain kinase facilitates endocytosis of synaptic
            vesicles at hippocampal boutons
  JOURNAL   J Neurochem 138 (1), 60-73 (2016)
   PUBMED   27062289
REFERENCE   4  (bases 1 to 7764)
  AUTHORS   Liang,Y., Cucchetti,M., Roncagalli,R., Yokosuka,T., Malzac,A.,
            Bertosio,E., Imbert,J., Nijman,I.J., Suchanek,M., Saito,T.,
            Wulfing,C., Malissen,B. and Malissen,M.
  TITLE     The lymphoid lineage-specific actin-uncapping protein Rltpr is
            essential for costimulation via CD28 and the development of
            regulatory T cells
  JOURNAL   Nat Immunol 14 (8), 858-866 (2013)
   PUBMED   23793062
REFERENCE   5  (bases 1 to 7764)
  AUTHORS   Hu,W. and Feng,P.
  TITLE     Myosin light chain kinase is involved in the mechanism of
            gastrointestinal dysfunction in diabetic rats
  JOURNAL   Dig Dis Sci 57 (5), 1197-1202 (2012)
   PUBMED   22302242
  REMARK    GeneRIF: The expression levels of Myosin light chain kinase in the
            pylorus and ileum of the diabetic rats were decreased and the
            changes of Myosin light chain kinase expression in these tissues
            were partially reversed after treatment with insulin.
REFERENCE   6  (bases 1 to 7764)
  AUTHORS   Gao,L., Grant,A., Halder,I., Brower,R., Sevransky,J., Maloney,J.P.,
            Moss,M., Shanholtz,C., Yates,C.R., Meduri,G.U., Shriver,M.D.,
            Ingersoll,R., Scott,A.F., Beaty,T.H., Moitra,J., Ma,S.F., Ye,S.Q.,
            Barnes,K.C. and Garcia,J.G.
  TITLE     Novel polymorphisms in the myosin light chain kinase gene confer
            risk for acute lung injury
  JOURNAL   Am J Respir Cell Mol Biol 34 (4), 487-495 (2006)
   PUBMED   16399953
REFERENCE   7  (bases 1 to 7764)
  AUTHORS   Shimizu,S., Yoshida,T., Wakamori,M., Ishii,M., Okada,T.,
            Takahashi,M., Seto,M., Sakurada,K., Kiuchi,Y. and Mori,Y.
  TITLE     Ca2+-calmodulin-dependent myosin light chain kinase is essential
            for activation of TRPC5 channels expressed in HEK293 cells
  JOURNAL   J Physiol 570 (Pt 2), 219-235 (2006)
   PUBMED   16284075
REFERENCE   8  (bases 1 to 7764)
  AUTHORS   Russo,J.M., Florian,P., Shen,L., Graham,W.V., Tretiakova,M.S.,
            Gitter,A.H., Mrsny,R.J. and Turner,J.R.
  TITLE     Distinct temporal-spatial roles for rho kinase and myosin light
            chain kinase in epithelial purse-string wound closure
  JOURNAL   Gastroenterology 128 (4), 987-1001 (2005)
   PUBMED   15825080
REFERENCE   9  (bases 1 to 7764)
  AUTHORS   Dulyaninova,N.G., Patskovsky,Y.V. and Bresnick,A.R.
  TITLE     The N-terminus of the long MLCK induces a disruption in normal
            spindle morphology and metaphase arrest
  JOURNAL   J Cell Sci 117 (Pt 8), 1481-1493 (2004)
   PUBMED   15020676
REFERENCE   10 (bases 1 to 7764)
  AUTHORS   Shen,M.R., Furla,P., Chou,C.Y. and Ellory,J.C.
  TITLE     Myosin light chain kinase modulates hypotonicity-induced Ca2+ entry
            and Cl- channel activity in human cervical cancer cells
  JOURNAL   Pflugers Arch 444 (1-2), 276-285 (2002)
   PUBMED   11976941
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000011.1.
            
            On Jul 9, 2010 this sequence version replaced NM_001105874.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence because insufficient rat transcript evidence was
            available to represent the gene. The genomic coordinates used for
            the transcript record were based on alignments and orthologous
            data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA5760383, SAMEA5760386
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-64                JAXUCZ010000011.1  79498511-79498574   c
            65-232              JAXUCZ010000011.1  79468753-79468920   c
            233-440             JAXUCZ010000011.1  79422773-79422980   c
            441-489             JAXUCZ010000011.1  79413183-79413231   c
            490-640             JAXUCZ010000011.1  79412220-79412370   c
            641-806             JAXUCZ010000011.1  79410724-79410889   c
            807-825             JAXUCZ010000011.1  79408381-79408399   c
            826-1337            JAXUCZ010000011.1  79406857-79407368   c
            1338-1544           JAXUCZ010000011.1  79406114-79406320   c
            1545-1679           JAXUCZ010000011.1  79400123-79400257   c
            1680-1832           JAXUCZ010000011.1  79396840-79396992   c
            1833-1970           JAXUCZ010000011.1  79384264-79384401   c
            1971-2168           JAXUCZ010000011.1  79382344-79382541   c
            2169-2418           JAXUCZ010000011.1  79381474-79381723   c
            2419-2493           JAXUCZ010000011.1  79375370-79375444   c
            2494-3617           JAXUCZ010000011.1  79373772-79374895   c
            3618-3734           JAXUCZ010000011.1  79366915-79367031   c
            3735-3821           JAXUCZ010000011.1  79357983-79358069   c
            3822-3872           JAXUCZ010000011.1  79343679-79343729   c
            3873-4000           JAXUCZ010000011.1  79342810-79342937   c
            4001-4154           JAXUCZ010000011.1  79339719-79339872   c
            4155-4457           JAXUCZ010000011.1  79330978-79331280   c
            4458-4490           JAXUCZ010000011.1  79322205-79322237   c
            4491-4584           JAXUCZ010000011.1  79322012-79322105   c
            4585-4788           JAXUCZ010000011.1  79320531-79320734   c
            4789-5006           JAXUCZ010000011.1  79313275-79313492   c
            5007-5130           JAXUCZ010000011.1  79311059-79311182   c
            5131-5283           JAXUCZ010000011.1  79303486-79303638   c
            5284-5407           JAXUCZ010000011.1  79301308-79301431   c
            5408-5537           JAXUCZ010000011.1  79295138-79295267   c
            5538-5669           JAXUCZ010000011.1  79293579-79293710   c
            5670-7764           JAXUCZ010000011.1  79288243-79290337   c
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q22"
     gene            1..7764
                     /gene="Mylk"
                     /note="myosin light chain kinase"
                     /db_xref="GeneID:288057"
                     /db_xref="RGD:1310915"
     exon            1..64
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..5926
                     /gene="Mylk"
                     /EC_number="2.7.11.18"
                     /note="myosin, light polypeptide kinase"
                     /codon_start=1
                     /product="myosin light chain kinase, smooth muscle"
                     /protein_id="NP_001099344.2"
                     /db_xref="GeneID:288057"
                     /db_xref="RGD:1310915"
                     /translation="MPGQLPGKTMGDVKLFASSHMSKTSHSVDPSRVSSLPLTEAPAF
                     ILPPRNLCVKEGATAKFEGRVRGHPEPQVTWHRNGQTITNGGRFLLDGGVRGTFSLVI
                     RGVREEDKGKYTCEASNGSGARQVTVELTVEGDLMKKHGQPVLSKASGFPGETRPSIW
                     GECPPKFATKLGRAVVKEGQMGRFSCKITGRPPPQVTWLKGNVPLQPSARVSMSEKNG
                     MQILEIHEVTQDDMGTYTCMVVNGSGKASMSAELSIPGLDNATRLSRGTKDPSPDIRK
                     EVTNGISKDPETVAESKNCSSPQKSNFLAQATNSHLKSLQQPKLKPCEDSPRKVLQSS
                     VLQKTSSTIVLQASKVQPEVRAAGIGASGPGEERKSLTAPRQATPPTRQCGLGGSVGN
                     KFITGNIPIENQRESRFPKFESQPQSQEVTEGQTVKFTCEVSGIPKPEVGWFLEGIPV
                     RRQEGIVEIYEDGTSHYLCLPRAGTRDSGRYSCTASNTLGQVSCSWTLRVDRPSLTQT
                     APSFSSVLKDSVVIEGQDFVLRCSVRGTPAPQVTWLLNGQPIQFARSICEAGEAELHI
                     QDALPEDRGTYTCLAENALGQVSCSATVTVQEKKGEGERAHRLPPAPSKLFAPIFLQG
                     LSDLKVMDGSQVTMTVQVSGNPPPEVIWLHDGNEIQESEDFHFEQKGGWHSLCIQEVF
                     PEDTGTYTCEAWNSAGEVRTQAILTVQEPHDGTQPWFISKPRSVTASLGQSVLISCAI
                     AGDPFPTVHWLRDGKALSRDHGHFELLQNEDVFTLVLKNVQPWHAGQYEILLKNRVGE
                     CSCQVSLMLHKGPSRVPPPRGREPASCEGLCGGGVGAHGDADRDGTLRPCWPARGQGW
                     PEEEDGEDVRGLLKRRVETRLHTEEAIRQQEVGQLDFRDLLGKKVSTKTVSEDDLKDI
                     PAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTPKTPVPEKVPPQKA
                     ATPDFRSVLGSKKKSPAENGGNTAEVLNVKAGESPTPAGNAQPVGALKPIGNAKPAET
                     LKSVGNAKPAETLKPVGNAKPAETLKPVGNVKPAETLKPIANAQPSGSPKPIANAQPI
                     ETQKSVANAKSAETPKLAGKEELKEVRNDVNCKKAHVGATGNEKRPESQGSAPVFKEK
                     LQDVHVAEGEKLLLQCQVTSDPPATVTWTLNGKTLKTTKFIILAQEGSLFSVSIEKAL
                     PEDRGLYKCVAKNSAGQAECSCQVTVDDAGTSENTKAPEMKSRRPKSSLTPVLGTESD
                     ATVKKKPASKTPTKAAMPPQIIQFPEDQKVRAGEPVELFGKVTGTQPITCTWMKFRKQ
                     IQESENIRVENSESGSKLTILAARQEHCGCYTLVVENKLSSRQAQVNLTVVDKPDPPA
                     GTPCASDIRSSSLTLSWYGSSYDGGSAVQSYNVEIWDTEDKVWTELATCRSTSFNVQD
                     LLPDREYKFRVRAVNVYGTSEPSQESELTAVGEKPEEPKDEVEVSDDDEKEPEVDYRT
                     VTVNTEQKVSDVYDIEERLGSGKFGQVFRLVEKRTGKIWAGKFFKAYSAKEKENIRQE
                     ISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMR
                     QISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTP
                     EFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDE
                     AFDEISEDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMA
                     RRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPINAEKLESEEDVSQAFLEA
                     VAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQI
                     DYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEEGGEEEE
                     EEE"
     exon            65..232
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            233..440
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            441..489
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            490..640
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            641..806
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            807..825
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            826..1337
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1544
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1679
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            1680..1832
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            1833..1970
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            1971..2168
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            2169..2418
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2493
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            2494..3617
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            3618..3734
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            3735..3821
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            3822..3872
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            3873..4000
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            4001..4154
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            4155..4457
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            4458..4490
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            4491..4584
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            4585..4788
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            4789..5006
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            5007..5130
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            5131..5283
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            5284..5407
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            5408..5537
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            5538..5669
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
     exon            5670..7764
                     /gene="Mylk"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gactctccct gggtgtccaa gagagccttg atgtgcaagc atgcctggcc agttacctgg
       61 gaagaccatg ggggacgtga aactgtttgc ctcgtcgcac atgtccaaaa cctcccacag
      121 tgtggatccc tcgagggtca gctctctgcc tctcaccgag gcccctgctt tcattctgcc
      181 tcctcggaac ctctgcgtca aagaaggagc caccgccaag tttgaaggga gggtccgggg
      241 ccacccagag ccccaggtga catggcacag aaatgggcaa accatcacca atggcggccg
      301 cttcctgctg gacggtgggg tgcgggggac tttcagcctc gtcatccgtg gtgtccgtga
      361 ggaggacaag ggcaagtaca cctgtgaagc tagcaacggc agtggcgccc gccaggtgac
      421 cgtggagctg actgtggaag gggatcttat gaagaagcat ggtcagcccg ttctttctaa
      481 agcctcagga tttccagggg agactcgtcc tagcatctgg ggtgagtgcc cgccaaagtt
      541 tgccaccaag ctgggcagag ctgtggtcaa agaagggcag atggggcgat tttcctgcaa
      601 gatcactggc cggcctccac cacaggtcac ctggctcaag ggaaatgtcc cgttgcagcc
      661 aagtgcccga gtgtctatgt cagagaagaa tggcatgcag atcctggaaa tccacgaggt
      721 cactcaggac gacatgggca cgtacacatg catggtggtg aatgggtcag ggaaagcctc
      781 catgtcagcg gagctctcca tcccaggttt ggacaatgcc accaggttgt caagaggaac
      841 caaggacccc agtccagaca tcaggaagga ggtgaccaat ggaatctcca aggacccaga
      901 gactgtggct gaaagtaaga actgttcaag tccccagaag agcaacttct tagcccaggc
      961 caccaacagt cacctcaagt ccctgcagca gccaaagctg aagccgtgtg aagattcccc
     1021 cagaaaggtc ctgcagtcct ccgtcctgca gaagacctcc agcaccattg ttctgcaggc
     1081 ctcaaaagtc cagccagaag tgagagcagc gggcataggg gcctccggtc ctggggaaga
     1141 gaggaagagt ctgactgccc ctcggcaagc cacaccccct accagacagt gtggcctggg
     1201 aggctcagtt gggaacaaat ttatcacggg gaacatcccc atagagaacc aaagggagtc
     1261 aagatttccc aagtttgaga gtcagcccca aagccaagaa gtcactgaag gtcaaacagt
     1321 caagttcacc tgtgaggttt cagggatacc aaagcctgaa gtgggttggt tcctggaagg
     1381 catccccgtg aggagacaag aaggcatcgt tgagatttat gaagacggta catcccatta
     1441 cctgtgtctg ccgagagccg ggacgagaga cagcgggaga tacagctgca cagcatccaa
     1501 tacccttggc caagtgtcct gcagttggac gctccgggtg gaccggccaa gcttgacgca
     1561 gacagcccct tccttctcta gtgttctgaa ggacagtgtt gtcattgaag gccaggattt
     1621 tgtgctacgg tgctctgtac gggggacccc agcaccccag gtcacttggc tgctcaatgg
     1681 gcagcccatc cagtttgctc gatccatctg tgaggctggc gaggccgagc tccacatcca
     1741 ggatgccctg cccgaggacc gtggcaccta cacctgcctg gctgagaatg ccttggggca
     1801 ggtgtcctgc agtgccacgg tcacggtcca agaaaagaaa ggcgaaggag agagggcgca
     1861 ccgtctgccc ccagctccca gcaagctctt cgctcccatc ttcctgcagg gtctctctga
     1921 cctcaaagtc atggatggaa gccaggtcac tatgacagtc caggtgtcag gaaacccacc
     1981 tccagaagtc atatggcttc acgacgggaa cgagatccag gagtcagagg acttccactt
     2041 cgagcagaaa ggtggctggc acagcctatg tatccaggaa gtgttcccgg aggacacggg
     2101 cacatacacc tgtgaggcct ggaacagcgc tggggaggtc cgcacccagg ccatactcac
     2161 cgtgcaagag ccccatgatg gcacccagcc ctggttcatc agcaagcctc gttcggtgac
     2221 agcctccctg ggccagagtg tcctcatctc ctgcgccata gctggagacc cctttcccac
     2281 tgtgcactgg ctccgagatg gcaaagccct ctccagggac catggccact ttgagctgct
     2341 ccagaatgag gacgtgttca ccctggttct aaagaacgtg cagccctggc atgccggcca
     2401 gtatgagatc ctgctcaaga accgggttgg cgagtgcagt tgccaagtgt cactgatgct
     2461 gcacaaaggt ccttccagag tcccaccacc acgggggcgg gagcctgcca gctgtgaggg
     2521 cctctgtggt ggaggagttg gtgctcatgg tgatgctgac cgtgatggga ccctgaggcc
     2581 ctgctggcca gcaagagggc agggttggcc tgaggaggaa gacggagaag acgtgagggg
     2641 gctgttgaag agacgggtgg agaccaggct gcacacagaa gaggccatcc gccagcagga
     2701 ggtggggcag ctggattttc gagacctcct gggaaagaag gtgagcacaa agaccgtatc
     2761 agaagacgac ctgaaggaca tcccagccga gcagatggat ttccgcgcca accttcagcg
     2821 gcaagtgaag ccaaagactg tgtctgagga agagaggaag gtgcatagcc cccagcaggt
     2881 tgacttccgg tctgtcctag ccaagaaggg gacccctaag acccctgtcc ctgagaaggt
     2941 gccacctcaa aaagctgcca ccccagactt tcgctcagtg ctaggtagca agaagaagtc
     3001 gcctgcggag aatgggggca acactgctga ggtcttgaat gtcaaggctg gggaaagccc
     3061 cacgcctgca ggcaatgcac agccagtcgg ggccctgaaa cccataggca acgccaagcc
     3121 tgctgagacc ctgaagtccg tgggcaatgc caaaccagca gagaccctga agcccgtggg
     3181 gaatgccaag ccagcagaga ccctgaaacc cgtaggtaat gtcaagcctg ccgagaccct
     3241 gaagcccata gccaacgcac agccttcagg gtccccaaaa cccatagcca acgcacagcc
     3301 tattgagacc cagaagtctg tggccaatgc caagtctgcc gagaccccga aactagcggg
     3361 gaaagaagaa ctcaaggagg ttaggaatga tgtgaactgt aagaaagcgc atgtcggagc
     3421 caccggcaat gaaaaaagac ctgagagcca aggctcggcc ccagttttca aggaaaagct
     3481 acaagatgtc catgtggctg agggcgagaa gctgctactc cagtgccagg tgacctctga
     3541 tcctccggcc actgtcacct ggacactgaa tggaaagaca ctcaagacca caaaattcat
     3601 catcctcgcc caagaaggct cactcttctc cgtctccatc gagaaggcac tgcctgagga
     3661 caggggtcta tacaagtgtg tagccaagaa cagcgctggc caggcggaat gttcctgcca
     3721 agtcactgtg gacgatgctg ggaccagcga gaacaccaag gccccggaga tgaaatcccg
     3781 gagacccaag agctctctta cgcctgtgct aggaacggaa agtgatgcta ctgtgaaaaa
     3841 gaaacctgcc tccaagacac ctacaaaagc agctatgcct cctcagatca tccagttccc
     3901 tgaggaccag aaggtgcgtg ctggagagcc agtggagctg tttgggaaag tgaccggtac
     3961 ccagcccatc acctgcacgt ggatgaagtt ccgaaagcag atccaggaga gcgagaacat
     4021 cagggtggag aacagtgaga gcggcagcaa gctcaccatc ctggctgcac ggcaggagca
     4081 ctgtggctgc tacacactgg tggtggagaa caagctgagc agcagacagg cccaagtcaa
     4141 cctcacggtt gtggataagc cagacccccc ggctggcaca ccttgtgctt ctgacatacg
     4201 gagttcctcg ctcaccctgt cctggtatgg ctcctcgtat gatgggggca gtgccgtaca
     4261 gtcctacaat gttgagatct gggacacgga ggacaaggtg tggacagagc tagccacgtg
     4321 ccgcagcacc tctttcaatg tccaagacct gctgcctgac cgcgagtata aattccgagt
     4381 acgtgcagtc aacgtctacg gaaccagcga gccgagccag gagtctgagc tcaccgcagt
     4441 gggagagaag cctgaagagc caaaggatga agtggaagtg tccgatgatg acgaaaagga
     4501 gcctgaggtt gattaccgga cggtgacggt gaacactgaa cagaaagtat ctgatgtata
     4561 tgacattgaa gagcgactag gatctgggaa atttggacaa gtcttccgac ttgttgaaaa
     4621 gagaactgga aaaatctggg cagggaaatt tttcaaggcc tattccgcca aggagaaaga
     4681 aaacatccgt caggagatca gcatcatgaa ctgtctccac caccccaagc tggtccagtg
     4741 cgtggatgcc ttcgaagaaa aggccaacat cgtcatggtt ctggagattg tgtcaggggg
     4801 tgagctattt gagcgcatca ttgatgagga cttcgagcta acagagcggg agtgcatcaa
     4861 gtacatgagg cagatttcgg aaggggtgga gtacatccac aagcagggca tcgtgcatct
     4921 ggacctcaag cctgagaaca ttatgtgtgt caacaagaca ggcaccagga tcaagctcat
     4981 tgacttcggc ctggcacgaa gactcgagaa cgctgggtct ctgaaggtcc tctttgggac
     5041 cccagagttt gtggctcctg aggtgatcaa ctatgagccc atcggctacg ccacagacat
     5101 gtggagcatc ggggtcatct gctatattct ggtcagcgga ctttccccct tcatgggtga
     5161 caatgataac gaaactttag ccaatgtcac ttcagctacc tgggacttcg acgacgaggc
     5221 gttcgatgag atctcggaag atgcgaagga cttcatcagt aacttgctaa agaaagatat
     5281 gaaaaaccgt ctggactgca cccagtgcct tcagcaccca tggctgatga aagacaccaa
     5341 gaacatggag gccaagaagc tgtccaagga ccggatgaag aaatacatgg caagaaggaa
     5401 gtggcagaaa acaggcaatg ctgtgagagc cattgggaga ctgtcctcta tggcaatgat
     5461 ctcagggctc agtggcagga aatcttcaac agggtcacca accagcccga tcaatgcaga
     5521 gaagctagag tctgaagaag atgtctccca ggccttcctc gaggctgttg ctgaggaaaa
     5581 gccccatgta aagccctact tctctaagac cattcgtgac ctggaggttg tggagggaag
     5641 cgctgctaga tttgactgca agatcgaagg atacccagac cctgaggttg tctggttcaa
     5701 ggatgaccag tcaatcaggg agtcccgcca cttccagata gactacgatg aagacgggaa
     5761 ctgctcgcta attatcagtg atgtctgtgg ggatgatgac gccaagtaca cctgcaaggc
     5821 tgtcaacagc ctcggagaag ccacctgcac cgcggagctc atcgtggaga ccatggagga
     5881 aggggaaggg gaggaggggg gggaggagga ggaggaggag gagtgaagcc aagccaaaca
     5941 gaagcaggtc ctaagtcatt taaaaaggac taattcctta aagttcaaaa aaagtcaatc
     6001 taaaggggca cctggtgtgg tagattacct agttaggcca tttggaggct gattcgtcag
     6061 caagggcggt tgatacctag gagatattga tgggcattac tttaaattag atagcacctt
     6121 acaacactag tgcagctcga ttccatttga gggagatcac cagcaacttg tctaaccgat
     6181 ttgatttgag agagaaaata gctaaaagaa atactcacct ggaaaagcca gacgtttccc
     6241 aatttaatgc accctgaaaa ataaagtcat catcagggcc ctgagcctcc tgcagcagct
     6301 caacacagat tccccggaga ctgaatatgc cccacccacc cacccaccca atccggagag
     6361 ccagccttgt cccatcctgc agtgtcttcg tctgaaagca gttgagccat tctgactcta
     6421 gaggcacatt gccttccaac cctacacttc caagctctct gtctctctcc ccattccacc
     6481 tgctgggttt cctggctctg atcctggcat gagttacagc aatgaggttt caattacgtt
     6541 tcttccatcc tgaaaccaga ttcccctgct cgtggatgac tctgacttcc ttggagtggg
     6601 gggaatctcc tctcctctaa aacccttagg gaactaaact tactcctctt ttcgggccat
     6661 ctggcagctt cagatatttg catgagaatt catgaaggag aaaaagaaaa tccatgctaa
     6721 tgtggccctc ccttaaacca ggattcctat ttgtgccagt taacaaactg tcagcctgcg
     6781 tgtgcagtaa agagtcttgc acccggatgt agaaagccag tttgctgaag tgctggttta
     6841 aattatttat tgggcgctat gaaactgctg tcaactggtg agcaaaggta gaactcggtg
     6901 tactttggaa tctccttcta gacagtctga gtttaataca tcttccttgt cgacactctt
     6961 caaagagctt ggcctgcccc ctggggtcca cctttatttt tcttcttgaa tgtgcatccc
     7021 ctttgctccc cgaccttgag ctatattctt agacctgttc agcacactca gacttgcatc
     7081 tttagcaatt ttttttaact tcctttcact tcattggcac ttaattattt ttccttataa
     7141 aactttttgg aggtcataaa caaagaccat aaaccaatat cttagtatgt ttcctctgtg
     7201 tgtgcgtgtg cgtgcgtgtc taacattcca aatacttttt ttttcttttc cactgtttgt
     7261 aaagtgcagc aaattagtat ttcaaaggat gttttaatag ttccttaaaa accgaactta
     7321 aactacttcc gtgcagtcct acacagtgcc aacattagag agagatgctg cttcgtctgc
     7381 ttagcttcca cccttccttg gctgtttgct gctgctaaat tgccagtatt cccctgtttt
     7441 gcttcttaag cagttacaat atctttcatt atagtcacaa tattgccacg gtttattata
     7501 ataaaggggt tttaatttga tttagagcat tcaaagtgtt tttttcatca cttggtgttt
     7561 atattataat ctttgtgtgt atgttgtgtg tatgtgtgca tgggtggtca tacgtgtgtt
     7621 tatagggtaa tgccttcttg ggattgtgtt attgttctct cagtttatta tgccatctaa
     7681 atggtgaaca agaactctat ggctgtagtt aactcacaat ttttaaataa aggataccac
     7741 agtgcatggt gtttgttcaa tctt
//
