LOCUS       NM_001107344            3069 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus myosin regulatory light chain interacting protein
            (Mylip), mRNA.
ACCESSION   NM_001107344 XM_001059193 XM_225220
VERSION     NM_001107344.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Wang,W., Zhang,Z.Z., Wu,Y., Wang,R.Q., Chen,J.W., Chen,J.,
            Zhang,Y., Chen,Y.J., Geng,M., Xu,Z.D., Dai,M., Li,J.H. and Pan,L.L.
  TITLE     (-)-Epigallocatechin-3-Gallate Ameliorates Atherosclerosis and
            Modulates Hepatic Lipid Metabolic Gene Expression in Apolipoprotein
            E Knockout Mice: Involvement of TTC39B
  JOURNAL   Front Pharmacol 9, 195 (2018)
   PUBMED   29593532
  REMARK    Erratum:[Front Pharmacol. 2018 May 01;9:459. doi:
            10.3389/fphar.2018.00459.. PMID: 29745376]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Liu,S. and Vaziri,N.D.
  TITLE     Role of PCSK9 and IDOL in the pathogenesis of acquired LDL receptor
            deficiency and hypercholesterolemia in nephrotic syndrome
  JOURNAL   Nephrol Dial Transplant 29 (3), 538-543 (2014)
   PUBMED   24166456
  REMARK    GeneRIF: LDLR deficiency, hypercholesterolemia and elevated plasma
            LDL in NS are associated with upregulation of PCSK9 and IDOL.
REFERENCE   3  (bases 1 to 3069)
  AUTHORS   Do,H.T., Bruelle,C., Tselykh,T., Jalonen,P., Korhonen,L. and
            Lindholm,D.
  TITLE     Reciprocal regulation of very low density lipoprotein receptors
            (VLDLRs) in neurons by brain-derived neurotrophic factor (BDNF) and
            Reelin: involvement of the E3 ligase Mylip/Idol
  JOURNAL   J Biol Chem 288 (41), 29613-29620 (2013)
   PUBMED   23990472
  REMARK    GeneRIF: Data indicate that down-regulation of Mylip/Idol using
            shRNAs abrogated the decrease in VLDLRs induced by Reelin.
REFERENCE   4  (bases 1 to 3069)
  AUTHORS   Zhang,L., Fairall,L., Goult,B.T., Calkin,A.C., Hong,C.,
            Millard,C.J., Tontonoz,P. and Schwabe,J.W.
  TITLE     The IDOL-UBE2D complex mediates sterol-dependent degradation of the
            LDL receptor
  JOURNAL   Genes Dev 25 (12), 1262-1274 (2011)
   PUBMED   21685362
REFERENCE   5  (bases 1 to 3069)
  AUTHORS   Zelcer,N., Hong,C., Boyadjian,R. and Tontonoz,P.
  TITLE     LXR regulates cholesterol uptake through Idol-dependent
            ubiquitination of the LDL receptor
  JOURNAL   Science 325 (5936), 100-104 (2009)
   PUBMED   19520913
REFERENCE   6  (bases 1 to 3069)
  AUTHORS   Ebstein,F., Lange,N., Urban,S., Seifert,U., Kruger,E. and
            Kloetzel,P.M.
  TITLE     Maturation of human dendritic cells is accompanied by functional
            remodelling of the ubiquitin-proteasome system
  JOURNAL   Int J Biochem Cell Biol 41 (5), 1205-1215 (2009)
   PUBMED   19028597
REFERENCE   7  (bases 1 to 3069)
  AUTHORS   Bornhauser,B.C., Johansson,C. and Lindholm,D.
  TITLE     Functional activities and cellular localization of the ezrin,
            radixin, moesin (ERM) and RING zinc finger domains in MIR
  JOURNAL   FEBS Lett 553 (1-2), 195-199 (2003)
   PUBMED   14550572
REFERENCE   8  (bases 1 to 3069)
  AUTHORS   Olsson,P.A., Korhonen,L., Mercer,E.A. and Lindholm,D.
  TITLE     MIR is a novel ERM-like protein that interacts with myosin
            regulatory light chain and inhibits neurite outgrowth
  JOURNAL   J Biol Chem 274 (51), 36288-36292 (1999)
   PUBMED   10593918
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000017.1.
            
            On Sep 21, 2012 this sequence version replaced NM_001107344.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360186.1061984.1,
                                           SRR26360190.1841985.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-313               JAXUCZ010000017.1  19514159-19514471   c
            314-504             JAXUCZ010000017.1  19512860-19513050   c
            505-690             JAXUCZ010000017.1  19500996-19501181   c
            691-888             JAXUCZ010000017.1  19499544-19499741   c
            889-1053            JAXUCZ010000017.1  19498690-19498854   c
            1054-1474           JAXUCZ010000017.1  19496671-19497091   c
            1475-3069           JAXUCZ010000017.1  19492826-19494420   c
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17p14"
     gene            1..3069
                     /gene="Mylip"
                     /gene_synonym="MIR"
                     /note="myosin regulatory light chain interacting protein"
                     /db_xref="GeneID:306825"
                     /db_xref="RGD:1305761"
     exon            1..313
                     /gene="Mylip"
                     /gene_synonym="MIR"
                     /inference="alignment:Splign:2.1.0"
     CDS             227..1564
                     /gene="Mylip"
                     /gene_synonym="MIR"
                     /EC_number="2.3.2.27"
                     /note="idol; inducible degrader of the LDL-receptor;
                     RING-type E3 ubiquitin transferase MYLIP"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase MYLIP"
                     /protein_id="NP_001100814.2"
                     /db_xref="GeneID:306825"
                     /db_xref="RGD:1305761"
                     /translation="MLCYVTRPDAVLMEVEVEAKANGEDCLNQVCRRLGIIEVDYFGL
                     QFTGSKGESLWLNLRNRISQQMDGLAPYRLKLRVKFFVEPHLILQEQTRHIFFLHIKE
                     SLLAGHLQCSPEQAVELSALLAQTKFGDYNQNTAQYSYEDLCEKELSSSTLNSIVGKH
                     KELEGISQASAEYQVLQIVSAMENYGIEWHAVRDSEGQKLLIGVGPEGISICKEDFSP
                     INRIAYPVVQMATQSGKNVYLTVTKESGNSIVLLFKMISTRAASGLYRAITETHAFYR
                     CDTVTSAVMMQYSRDLKGHLASLFLNENINLGKKYVFDIKRTSKEVYDHARRALYNAG
                     VVDLVSRNDQSPPSSPLKSSDSSMSCSSCEGLSCQQTRVLQEKLRKLKEAMLCMVCCE
                     EEINSTFCPCGHTVCCESCAAQLQSCPVCRSRVEHVQHVYLPTHTSLLNLTVI"
     exon            314..504
                     /gene="Mylip"
                     /gene_synonym="MIR"
                     /inference="alignment:Splign:2.1.0"
     exon            505..690
                     /gene="Mylip"
                     /gene_synonym="MIR"
                     /inference="alignment:Splign:2.1.0"
     exon            691..888
                     /gene="Mylip"
                     /gene_synonym="MIR"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1053
                     /gene="Mylip"
                     /gene_synonym="MIR"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1474
                     /gene="Mylip"
                     /gene_synonym="MIR"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..3069
                     /gene="Mylip"
                     /gene_synonym="MIR"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagtggggct gtcggagcgg cgcggccgtg tagctcccgg gaactggctg tcgtgggggg
       61 tggcggggac gcgagtggcg gctgcgtggg gtgcagggcg ggtgaccgca cggctgcacc
      121 ttcctcgcgg tgcccggagc cgacttggag caattgcagt gaggcgacag ccccggcgca
      181 caccggagaa gaagcggccg tggcggcggt ggcggcggcc ccagccatgc tgtgctatgt
      241 gacgaggccg gacgcggtgc tgatggaggt ggaggtggag gcaaaagcca acggcgagga
      301 ctgtctcaac caggtgtgca ggcgattggg aatcatagaa gttgattatt ttgggctgca
      361 gttcacgggt agcaaaggtg aaagcttatg gctgaatctg agaaaccgga tctcccagca
      421 gatggatggg ctggcacctt accggcttaa actgagggtc aagttctttg tggagcccca
      481 tctcatctta caggagcaga caaggcatat ctttttcttg cacattaaag agtccctctt
      541 ggcaggccac ctccagtgtt ccccagagca ggcagtggaa cttagtgccc tcctggccca
      601 gaccaaattc ggagactaca accagaacac tgcccaatac agctatgagg acctgtgtga
      661 gaaagagctc tccagctcca cattgaacag cattgttggg aagcataagg agctggaggg
      721 catcagccag gcttctgcag aataccaagt tctgcagatt gtgtcagcaa tggagaacta
      781 cggcatagag tggcatgccg tgagggacag cgaaggacag aaactcctca ttggggtcgg
      841 acctgaaggc atctcaattt gtaaagagga ctttagccct attaacagga tagcttatcc
      901 tgtggtgcag atggccaccc agtcaggaaa gaatgtctac ttgaccgtca ccaaggagtc
      961 cggtaacagc atcgtgctcc tgtttaaaat gatcagcacc cgagctgcca gtgggctcta
     1021 ccgagctatc acggaaacac atgcattcta caggtgtgac acagtcacca gtgccgtcat
     1081 gatgcagtac agtcgtgact tgaagggcca cttggcgtct ctgtttctga atgagaacat
     1141 taatcttggc aagaaatatg tctttgatat taaaagaaca tccaaagagg tatatgacca
     1201 tgccaggagg gctctgtaca acgccggtgt tgtggacctt gtctctcgaa atgaccagag
     1261 ccctcccagc tcgcctctga agtcctctga cagcagcatg agctgcagca gctgcgaggg
     1321 cctcagctgc cagcagacca gagtgctgca ggagaagctg cgcaaactga aggaagccat
     1381 gctgtgcatg gtgtgctgcg aggaggagat caactctacc ttttgcccct gtggccatac
     1441 tgtgtgctgt gagagctgtg cagcccagct gcagtcatgt cccgtctgca gatcacgtgt
     1501 ggaacatgtc cagcacgtct acctgcccac ccacaccagt cttctcaatc tgactgtcat
     1561 ctaatgcgtc ctgtgcttac tgggcatgcc atgtccccac aagctgcagt attgtaaact
     1621 agaagaagag taactctgtg aagaaatagt tcactctcaa cacccatttg ccatgagacg
     1681 tttccagaaa cgaggaggaa aggaaagaat gcgaccacac ctcttctgtg aggagaagca
     1741 acagttccca tggcaaccag gaagagatct ggaacatgga cacattcctt gggctttggg
     1801 gtttttttaa tgatcaagta aaggagtaga taaaattgtc tctgtcagtc aagtggcaac
     1861 atggcccaac cgtgggcacc tttaaggaaa tgatgtcata tgtctccttc acttgtcccg
     1921 aggcagcaga ttttgtaaga gttttaagga tttccttggt tctttttgta tggtcatgga
     1981 gcgctgaaca tttttaatag gatttttttt tttttgtctt aaagaaatag tcctcattag
     2041 aaagtcattt ctgtctttat aactcattca agaacaactg gaaagctggt agtttggaaa
     2101 gcaatcccgt gacttctcaa gggttgagag caacgtggtc agattggaac cagtctggct
     2161 gagagtcaac aggtaaccca ccgtgggtga cttcgctcct agctcccctg tttccctcac
     2221 tccacatccc atgcctttca ctgataaaaa tgctaccagt ttggttacat acatggcatg
     2281 gtcaagcact ccctgggctt tggagctagt ggactatttg cagatcggaa agggagagtg
     2341 gcagagaggc ttccttggaa gggaaggggg ggggagagag agagaaagag agagagagag
     2401 agagagagag agagagggga gccagagagc cagagtgagt tttcacttcc cccaagcact
     2461 cactccagca gcacccatgg gtgtcgccgt gcttgaagat caaactttct acagccttat
     2521 aggtttctag gttgtatctc ctctttgtgt ctgtcttaat tccctttgtt ggtgttttct
     2581 taggtcagtg cttccgtatt cattgtactg cctcctcggc atcttccaga ggtggggcca
     2641 cttcattatg tttctatatt cttcgtcata atttcacccc cacttgggca ttttggaagc
     2701 tagtgagcta gggggttttc tagggtgtcc ggaagcctag ctgacctcat cgggtgcaat
     2761 actagctaca ttaaagctag aaacctacac tgtcacttta ctgagctctg agtctacgtt
     2821 tcatattgcc ttaatgtagc agtaatgtgt ttatgcattt gtttctttgc acagacattt
     2881 tgtcagatat taaaactcta cttttttatg gcacatatta gcatataagc ctttattcca
     2941 agaggtattt attttttcac ttgtaaaaaa aaataatgtt tccacattta aaaaaaaatc
     3001 aactctgtta tatcctagag gacttctgtc ttttatattc aggataataa agactttaaa
     3061 gcaaactgg
//
