LOCUS       NM_017343               1212 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus myosin light chain 12B (Myl12b), mRNA.
ACCESSION   NM_017343 XM_346859
VERSION     NM_017343.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Toepfer,C.N., Sikkel,M.B., Caorsi,V., Vydyanath,A., Torre,I.,
            Copeland,O., Lyon,A.R., Marston,S.B., Luther,P.K., Macleod,K.T.,
            West,T.G. and Ferenczi,M.A.
  TITLE     A post-MI power struggle: adaptations in cardiac power occur at the
            sarcomere level alongside MyBP-C and RLC phosphorylation
  JOURNAL   Am J Physiol Heart Circ Physiol 311 (2), H465-H475 (2016)
   PUBMED   27233767
  REMARK    GeneRIF: Phosphorylation of myosin regulatory light chain (RLC)
            increased and remained elevated post-MI, while phosphorylation of
            myosin binding protein-C (MyBP-C) was initially depressed but then
            increased as the hearts became decompensated.
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Lu,Z., Ma,X.N., Zhang,H.M., Ji,H.H., Ding,H., Zhang,J., Luo,D.,
            Sun,Y. and Li,X.D.
  TITLE     Mouse myosin-19 is a plus-end-directed, high-duty ratio molecular
            motor
  JOURNAL   J Biol Chem 289 (26), 18535-18548 (2014)
   PUBMED   24825904
REFERENCE   3  (bases 1 to 1212)
  AUTHORS   Kiyomitsu,T. and Cheeseman,I.M.
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately
            position the spindle in anaphase
  JOURNAL   Cell 154 (2), 391-402 (2013)
   PUBMED   23870127
  REMARK    Erratum:[Cell. 2013 Sep 12;154(6):1401]
REFERENCE   4  (bases 1 to 1212)
  AUTHORS   Sutherland,C. and Walsh,M.P.
  TITLE     Myosin regulatory light chain diphosphorylation slows relaxation of
            arterial smooth muscle
  JOURNAL   J Biol Chem 287 (29), 24064-24076 (2012)
   PUBMED   22661704
  REMARK    GeneRIF: Findings conclude that phosphorylation at Thr(18)
            decreases the rates of LC(20) dephosphorylation and smooth muscle
            relaxation compared with LC(20) phosphorylated exclusively at
            Ser(19).
REFERENCE   5  (bases 1 to 1212)
  AUTHORS   Revenu,C., Ubelmann,F., Hurbain,I., El-Marjou,F., Dingli,F.,
            Loew,D., Delacour,D., Gilet,J., Brot-Laroche,E., Rivero,F.,
            Louvard,D. and Robine,S.
  TITLE     A new role for the architecture of microvillar actin bundles in
            apical retention of membrane proteins
  JOURNAL   Mol Biol Cell 23 (2), 324-336 (2012)
   PUBMED   22114352
REFERENCE   6  (bases 1 to 1212)
  AUTHORS   Wang,Y. and Chantler,P.D.
  TITLE     Functional analysis of individual brain myosin II isoforms through
            hybrid formation
  JOURNAL   FEBS Lett 348 (3), 244-248 (1994)
   PUBMED   8034049
REFERENCE   7  (bases 1 to 1212)
  AUTHORS   Ozaki,T., Asai,T. and Sakiyama,S.
  TITLE     Molecular cloning of a unique complementary DNA of rat myosin
            regulatory light chain and its elevated expression in
            v-src-transformed rat culture cell lines
  JOURNAL   Cell Growth Differ 4 (11), 939-945 (1993)
   PUBMED   8297799
REFERENCE   8  (bases 1 to 1212)
  AUTHORS   Feinstein,D.L., Durand,M. and Milner,R.J.
  TITLE     Expression of myosin regulatory light chains in rat brain:
            characterization of a novel isoform
  JOURNAL   Brain Res Mol Brain Res 10 (2), 97-105 (1991)
   PUBMED   1649372
REFERENCE   9  (bases 1 to 1212)
  AUTHORS   Grant,J.W., Taubman,M.B., Church,S.L., Johnson,R.L. and
            Nadal-Ginard,B.
  TITLE     Mammalian nonsarcomeric myosin regulatory light chains are encoded
            by two differentially regulated and linked genes
  JOURNAL   J Cell Biol 111 (3), 1127-1135 (1990)
   PUBMED   2391362
  REMARK    Erratum:[J Cell Biol 1990 Nov;111(5 Pt 1):2207]
REFERENCE   10 (bases 1 to 1212)
  AUTHORS   Taubman,M.B., Grant,J.W. and Nadal-Ginard,B.
  TITLE     Cloning and characterization of mammalian myosin regulatory light
            chain (RLC) cDNA: the RLC gene is expressed in smooth, sarcomeric,
            and nonmuscle tissues
  JOURNAL   J Cell Biol 104 (6), 1505-1513 (1987)
   PUBMED   3584239
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CF977112.1, BC060577.1, EV769828.1 and CO405920.1.
            
            On Sep 17, 2008 this sequence version replaced NM_017343.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ118700.1, FQ229195.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132262, SAMD00155333
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 CF977112.1         5-9
            6-261               BC060577.1         5-260
            262-262             EV769828.1         249-249
            263-1090            BC060577.1         262-1089
            1091-1212           CO405920.1         1-122               c
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q38"
     gene            1..1212
                     /gene="Myl12b"
                     /gene_synonym="Mrlc2; Mrlcb; Mylc2b"
                     /note="myosin light chain 12B"
                     /db_xref="GeneID:50685"
                     /db_xref="RGD:628855"
     exon            1..85
                     /gene="Myl12b"
                     /gene_synonym="Mrlc2; Mrlcb; Mylc2b"
                     /inference="alignment:Splign:2.1.0"
     exon            86..284
                     /gene="Myl12b"
                     /gene_synonym="Mrlc2; Mrlcb; Mylc2b"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..619
                     /gene="Myl12b"
                     /gene_synonym="Mrlc2; Mrlcb; Mylc2b"
                     /note="myosin regulatory light chain-B; MLC-2; MLC20;
                     myosin RLC-B; myosin regulatory light chain 20 kDa; myosin
                     regulatory light chain MRLC2; myosin, light chain 12B,
                     regulatory; myosin light chain, regulatory B"
                     /codon_start=1
                     /product="myosin regulatory light chain 12B"
                     /protein_id="NP_059039.2"
                     /db_xref="GeneID:50685"
                     /db_xref="RGD:628855"
                     /translation="MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQN
                     RDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDV
                     IRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFT
                     RILKHGAKDKDD"
     exon            285..446
                     /gene="Myl12b"
                     /gene_synonym="Mrlc2; Mrlcb; Mylc2b"
                     /inference="alignment:Splign:2.1.0"
     exon            447..1200
                     /gene="Myl12b"
                     /gene_synonym="Mrlc2; Mrlcb; Mylc2b"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtgcggcgc ctccactgtc ccgccgcagc ctccgctggt tgcagtcgcc ggtctggtcc
       61 tcttgtgcgt gcagcttcag gtcagatttg aaccgccacc atgtcgagca aaaaagcaaa
      121 gaccaagacc accaaaaagc gccctcagcg cgcaacgtcc aacgtgttcg ccatgtttga
      181 ccagtcccag atccaggagt tcaaagaggc cttcaacatg atcgaccaga accgggacgg
      241 cttcattgac aaggaggacc tgcacgacat gctcgcgtct ctggggaaga accccaccga
      301 cgcctacctg gacgccatga tgaacgaggc cccgggcccc atcaatttca ccatgttcct
      361 caccatgttt ggagagaagc tgaacggcac cgacccagag gacgtcatca gaaacgcctt
      421 cgcttgcttc gatgaggaag ccacaggcac catccaggag gattacctga gggagctgct
      481 gaccaccatg ggtgaccgct tcacagatga ggaagtggat gagctgtaca gggaggcccc
      541 catcgacaaa aagggcaatt tcaactacat cgagttcacg cgcatcctca agcacggagc
      601 gaaagacaaa gatgactgaa gagctgcagc tgacattcct gttgagtttc cttattcact
      661 tcatgtctca gacacgcacc ccataggacc tactgtgtgc cactttgtcc ctcagacacc
      721 ccctcccata gggcctactg tgtgccgctt agtccctcag acaccccctc ccatagggcc
      781 tactgtgtgc cacttagtcc ctcagacacc ccctcccata gggcctactg tgtgccactt
      841 tgtccctcag acaccccctc ccataggacc tactgtgtac cacttggttt ctcagacagc
      901 cccaccccca aaccccctaa ggacctactg cgggccactt agtttcacag cctttcctct
      961 tttttaatgt atttattcca gaccctttct gccacttagc atgtgtataa tcagactgga
     1021 atggggacaa tgttgtaaat tgtactgaaa atgagatgca aataaaaaaa tcaaccaatg
     1081 tgaaagccca ggaaaatata tcggcatttc tggttttgct ggattttttt acatttttat
     1141 ataataaaaa tgttattttg aaataaagat tatgctgact caagtggagt ataaaacaaa
     1201 aaaaaaaaaa aa
//
