LOCUS       NM_001135157            6041 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus myosin heavy chain 2 (Myh2), mRNA.
ACCESSION   NM_001135157 XM_001077462 XM_001077478 XM_001077494 XM_001077511
            XM_001077532 XM_001077547 XM_001077568 XM_001077587 XM_001077603
VERSION     NM_001135157.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Sharlo,K.A., Paramonova,I.I., Lvova,I.D., Mochalova,E.P.,
            Kalashnikov,V.E., Vilchinskaya,N.A., Tyganov,S.A.,
            Konstantinova,T.S., Shevchenko,T.F., Kalamkarov,G.R. and
            Shenkman,B.S.
  TITLE     Plantar Mechanical Stimulation Maintains Slow Myosin Expression in
            Disused Rat Soleus Muscle via NO-Dependent Signaling
  JOURNAL   Int J Mol Sci 22 (3), 1372 (2021)
   PUBMED   33573052
  REMARK    GeneRIF: Plantar Mechanical Stimulation Maintains Slow Myosin
            Expression in Disused Rat Soleus Muscle via NO-Dependent Signaling.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Tornavaca,O., Chia,M., Dufton,N., Almagro,L.O., Conway,D.E.,
            Randi,A.M., Schwartz,M.A., Matter,K. and Balda,M.S.
  TITLE     ZO-1 controls endothelial adherens junctions, cell-cell tension,
            angiogenesis, and barrier formation
  JOURNAL   J Cell Biol 208 (6), 821-838 (2015)
   PUBMED   25753039
REFERENCE   3  (bases 1 to 6041)
  AUTHORS   Mizui,T., Sekino,Y., Yamazaki,H., Ishizuka,Y., Takahashi,H.,
            Kojima,N., Kojima,M. and Shirao,T.
  TITLE     Myosin II ATPase activity mediates the long-term
            potentiation-induced exodus of stable F-actin bound by drebrin A
            from dendritic spines
  JOURNAL   PLoS One 9 (1), e85367 (2014)
   PUBMED   24465547
  REMARK    GeneRIF: Data suggest that the myosin-II mediated drebrin A-actin
            exodus might be an initial event in chemical long-term potentiation
            (LTP) induction, triggering actin polymerization and spine
            enlargement.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6041)
  AUTHORS   Liang,Y., Cucchetti,M., Roncagalli,R., Yokosuka,T., Malzac,A.,
            Bertosio,E., Imbert,J., Nijman,I.J., Suchanek,M., Saito,T.,
            Wulfing,C., Malissen,B. and Malissen,M.
  TITLE     The lymphoid lineage-specific actin-uncapping protein Rltpr is
            essential for costimulation via CD28 and the development of
            regulatory T cells
  JOURNAL   Nat Immunol 14 (8), 858-866 (2013)
   PUBMED   23793062
REFERENCE   5  (bases 1 to 6041)
  AUTHORS   Galic,M., Jeong,S., Tsai,F.C., Joubert,L.M., Wu,Y.I., Hahn,K.M.,
            Cui,Y. and Meyer,T.
  TITLE     External push and internal pull forces recruit curvature-sensing
            N-BAR domain proteins to the plasma membrane
  JOURNAL   Nat Cell Biol 14 (8), 874-881 (2012)
   PUBMED   22750946
REFERENCE   6  (bases 1 to 6041)
  AUTHORS   Wylie,S.R. and Chantler,P.D.
  TITLE     Myosin IIA drives neurite retraction
  JOURNAL   Mol Biol Cell 14 (11), 4654-4666 (2003)
   PUBMED   12960431
REFERENCE   7  (bases 1 to 6041)
  AUTHORS   Martinsson,T., Oldfors,A., Darin,N., Berg,K., Tajsharghi,H.,
            Kyllerman,M. and Wahlstrom,J.
  TITLE     Autosomal dominant myopathy: missense mutation (Glu-706 --> Lys) in
            the myosin heavy chain IIa gene
  JOURNAL   Proc Natl Acad Sci U S A 97 (26), 14614-14619 (2000)
   PUBMED   11114175
REFERENCE   8  (bases 1 to 6041)
  AUTHORS   Lieber,R.L., Bodine,S.C., Burkholder,T.J., Pierotti,D.J. and
            Ryan,A.F.
  TITLE     Cloning and in situ hybridization of type 2A and 2B rat skeletal
            muscle myosin tail region: implications for filament assembly
  JOURNAL   Biochem Biophys Res Commun 197 (3), 1312-1318 (1993)
   PUBMED   8280148
REFERENCE   9  (bases 1 to 6041)
  AUTHORS   Larsson,L. and Moss,R.L.
  TITLE     Maximum velocity of shortening in relation to myosin isoform
            composition in single fibres from human skeletal muscles
  JOURNAL   J Physiol 472, 595-614 (1993)
   PUBMED   8145163
REFERENCE   10 (bases 1 to 6041)
  AUTHORS   DeNardi,C., Ausoni,S., Moretti,P., Gorza,L., Velleca,M.,
            Buckingham,M. and Schiaffino,S.
  TITLE     Type 2X-myosin heavy chain is coded by a muscle fiber type-specific
            and developmentally regulated gene
  JOURNAL   J Cell Biol 123 (4), 823-835 (1993)
   PUBMED   8227143
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On or before Sep 25, 2008 this sequence version replaced
            XM_001077547.1, XM_001077462.1, XM_001077511.1, XM_001077587.1,
            XM_001077478.1, XM_001077603.1, XM_001077532.1, XM_001077494.1,
            XM_001077568.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643295.38485.1,
                                           SRR26643296.26330.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                JAXUCZ010000010.1  52355739-52355760
            23-65               JAXUCZ010000010.1  52355905-52355947
            66-289              JAXUCZ010000010.1  52357765-52357988
            290-433             JAXUCZ010000010.1  52358094-52358237
            434-590             JAXUCZ010000010.1  52359599-52359755
            591-618             JAXUCZ010000010.1  52360770-52360797
            619-733             JAXUCZ010000010.1  52360880-52360994
            734-826             JAXUCZ010000010.1  52361079-52361171
            827-890             JAXUCZ010000010.1  52361713-52361776
            891-989             JAXUCZ010000010.1  52361895-52361993
            990-1093            JAXUCZ010000010.1  52362712-52362815
            1094-1232           JAXUCZ010000010.1  52363250-52363388
            1233-1351           JAXUCZ010000010.1  52363716-52363834
            1352-1501           JAXUCZ010000010.1  52363926-52364075
            1502-1672           JAXUCZ010000010.1  52365113-52365283
            1673-1982           JAXUCZ010000010.1  52365373-52365682
            1983-2062           JAXUCZ010000010.1  52366325-52366404
            2063-2150           JAXUCZ010000010.1  52367908-52367995
            2151-2268           JAXUCZ010000010.1  52368082-52368199
            2269-2392           JAXUCZ010000010.1  52369562-52369685
            2393-2529           JAXUCZ010000010.1  52369772-52369908
            2530-2785           JAXUCZ010000010.1  52371142-52371397
            2786-3028           JAXUCZ010000010.1  52372796-52373038
            3029-3205           JAXUCZ010000010.1  52373150-52373326
            3206-3351           JAXUCZ010000010.1  52373410-52373555
            3352-3442           JAXUCZ010000010.1  52373637-52373727
            3443-3832           JAXUCZ010000010.1  52373815-52374204
            3833-3959           JAXUCZ010000010.1  52374809-52374935
            3960-4078           JAXUCZ010000010.1  52375888-52376006
            4079-4275           JAXUCZ010000010.1  52376140-52376336
            4276-4459           JAXUCZ010000010.1  52376960-52377143
            4460-4625           JAXUCZ010000010.1  52377224-52377389
            4626-4750           JAXUCZ010000010.1  52377490-52377614
            4751-5059           JAXUCZ010000010.1  52377769-52378077
            5060-5263           JAXUCZ010000010.1  52378162-52378365
            5264-5389           JAXUCZ010000010.1  52379112-52379237
            5390-5560           JAXUCZ010000010.1  52379313-52379483
            5561-5665           JAXUCZ010000010.1  52379567-52379671
            5666-5761           JAXUCZ010000010.1  52379764-52379859
            5762-6041           JAXUCZ010000010.1  52381956-52382235
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q24"
     gene            1..6041
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /note="myosin heavy chain 2"
                     /db_xref="GeneID:691644"
                     /db_xref="RGD:620354"
     exon            1..22
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            23..65
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            66..289
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..5914
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /note="myosin, heavy polypeptide 2, skeletal muscle,
                     adult; 2A myosin heavy chain; myosin heavy chain type IIx;
                     myosin heavy chain IIa; myosin, heavy chain 2, skeletal
                     muscle, adult"
                     /codon_start=1
                     /product="myosin-2"
                     /protein_id="NP_001128629.1"
                     /db_xref="GeneID:691644"
                     /db_xref="RGD:620354"
                     /translation="MSSDSEMAVFGEAAPYLRKSEKERIEAQNRPFDAKTSVFVAEPK
                     ESFVKGTIQSKDAGKVTVKTEAGATLTVKEDQIFPMNPPKYDKIEDMAMMTHLHEPAV
                     LYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVAAYRGKKRQEAPPHIFSISD
                     NAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEVTSGKMQGT
                     LEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRV
                     TFQLKAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELMA
                     TDSAIDILGFTNDEKVSIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQG
                     LNSADLLKALCYPRVKVGNEYVTKGQTVEQVTNAVGALAKAMYEKMFLWMVTRINQQL
                     DTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEG
                     IEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSANF
                     QKPKVVKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNETVVGLYQKSSMKTLAYLF
                     SGAQTAEAEASSGGAAKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIP
                     NETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEG
                     QYIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQAM
                     CRGFLARVEYQKMVERRESIFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAETEKEM
                     ATMKEEFQKTKDELAKSEAKRKELEEKMVSLLKEKNDLQLQVQAEAEGLADAEERCDQ
                     LIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVE
                     KEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKA
                     KIKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDIENEKQQLDERLK
                     KKEFEMSNLQSKIEDEQAIGIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDL
                     SRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHA
                     DSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLEDQ
                     VSELKSKEEEQQRLINDLTTQRGRLQTESGEFSRQLDEKEALVSQLSRGKQAFTQQIE
                     ELKRQLEEEVKAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANSEVAQW
                     RTKYETDAIQRTEELEEAKKKLAQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLM
                     LDVERTNAACAALDKKQRNFDKILAEWKQKYEETHAELEASQKEARSLGTELFKMKNA
                     YEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKCELQAALE
                     EAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQSTLDA
                     EIRSRNDAIRIKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQLHLDDAL
                     RGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQ
                     NTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSA
                     HLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNVE
                     AVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNTNL
                     SKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE"
     exon            290..433
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            434..590
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            591..618
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            619..733
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            734..826
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            827..890
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            891..989
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1093
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1232
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1351
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1501
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1672
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1982
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2062
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..2150
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..2268
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2269..2392
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2393..2529
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2530..2785
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            2786..3028
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3029..3205
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3206..3351
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3352..3442
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3443..3832
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3833..3959
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            3960..4078
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4079..4275
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4276..4459
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4460..4625
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4626..4750
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            4751..5059
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            5060..5263
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            5264..5389
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            5390..5560
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            5561..5665
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            5666..5761
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
     exon            5762..6041
                     /gene="Myh2"
                     /gene_synonym="Myh1; Myh2a; MyHC-2A; MyHC-IIA"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tcctgcttta aaaagctcca aggaccctct tacttcccag ctgcaccttc tcatttgcca
       61 ataagggtct gtgagttcag cagccatgag ttccgactct gagatggccg ttttcgggga
      121 ggctgctcct tacctccgga agtctgaaaa ggagcgaatc gaggcccaga ataggccttt
      181 tgatgccaaa acatctgtct ttgtggcgga gcccaaggag tcctttgtca aaggaaccat
      241 tcagagcaaa gacgcaggga aagtgactgt gaaaactgaa gcaggagcga ccctgacggt
      301 gaaagaagac cagatcttcc ccatgaaccc tcccaagtac gacaagatcg aggacatggc
      361 catgatgacc cacctccacg agcccgctgt gctgtacaac ctcaaagagc gttacgcagc
      421 ctggatgatc tacacctact cgggcctctt ctgtgtcact gtcaacccct acaagtggct
      481 gccagtgtac aaccctgagg tggtggctgc ctaccgtggc aaaaagcgac aggaggcccc
      541 gccccacatc ttctccatct ctgacaatgc ctaccagttc atgctaactg accgggagaa
      601 tcagtcaatc ctgatcaccg gagaatccgg ggccgggaag actgtgaaca cgaagcgtgt
      661 catccagtac tttgcaacaa ttgcagtcac tggggagaaa aagaaggagg aggtgacttc
      721 tggcaaaatg caggggaccc tggaagatca aatcatcagt gccaaccctc tgctggaggc
      781 ctttgggaat gccaagaccg tgaggaatga caactcctct cgctttggta aattcatcag
      841 gattcacttt ggcaccacgg ggaaactggc atccgctgac atcgagacat atctgctaga
      901 gaagtcccgg gtcactttcc agcttaaggc ggaaaggagc taccatatat tttatcaaat
      961 cacatccaac aagaagccag agctgatcga aatgctgctg atcaccacaa acccatacga
     1021 ttacccattc gtcagtcaag gggagatcag tgtggccagc attgatgacc aggaagaact
     1081 gatggccacg gatagtgcta ttgacattct gggctttaca aatgatgaaa aggtctccat
     1141 ctataagctc acgggagctg tgatgcatta tgggaacatg aagttcaagc agaagcagcg
     1201 ggaagaacag gctgagccgg atggcacgga agtggctgac aaggctgcct atcttcaggg
     1261 tctgaactct gctgacctgc tcaaagccct ttgttacccc agggtcaagg tcggcaatga
     1321 gtacgtcacc aaaggccaga ctgtggagca ggtgaccaac gctgtgggtg ccctggccaa
     1381 ggccatgtac gagaagatgt tcctgtggat ggtcacccgc atcaaccagc agctggacac
     1441 caagcagccc aggcagtact tcatcggggt cttggacatc gctggctttg agatctttga
     1501 tttcaacagc ctggagcagc tgtgcatcaa cttcaccaac gagaaactgc aacagttttt
     1561 caaccaccac atgttcgtgc tggagcagga ggagtacaag aaggaaggca tcgagtggac
     1621 cttcatcgac ttcgggatgg acctggcggc ctgcatcgag ctcatcgaga agccgatggg
     1681 catcttctcc atcctggaag aggagtgcat gttccccaag gcgacagaca cctccttcaa
     1741 gaacaagctg tatgaacagc atcttggaaa gtccgccaac ttccagaagc ctaaggtggt
     1801 caaaggcaaa gccgaggccc acttctccct catccactat gcgggcaccg tggattacaa
     1861 catcactggc tggctggaca agaacaagga ccccctgaat gagaccgtgg tggggctgta
     1921 ccagaagtct tcaatgaaaa ctcttgctta cctcttctct ggagctcaaa ctgctgaagc
     1981 agaggcaagt agtggtggag ctgccaagaa aggtgctaag aagaaagggt cctccttcca
     2041 gacagtgtct gccctgttca gagagaactt gaacaagctg atgaccaacc tcaggagcac
     2101 ccaccctcat tttgtgaggt gtatcatccc caatgaaacc aagacacctg gtgccatgga
     2161 gcacgaactg gtcctgcacc agctgaggtg taacggggtg ctagaaggca tccgcatctg
     2221 caggaagggg ttccccagca ggatcctcta cgcagacttc aagcagagat acaaggtgtt
     2281 aaatgcaagt gccatccctg agggtcagta catagatagc aagaaggctt ctgagaagct
     2341 ccttggctcc atcgacattg accacactca gtataagttt ggtcacacca aggtcttttt
     2401 caaagctggt cttctggggc tcctagagga gatgagagat gacaagttgg cccagctgat
     2461 tacccggacg caggccatgt gcagagggtt cttggcaaga gtggagtacc agaagatggt
     2521 ggagcgaaga gagtccatct tctgcatcca gtacaacatc cgcgccttca tgaacgtcaa
     2581 gcactggccc tggatgaaac tcttcttcaa gatcaaacca ctgctgaaga gcgctgagac
     2641 cgagaaggag atggccacca tgaaggagga gttccagaaa accaaggatg aacttgccaa
     2701 atcggaggca aagaggaagg aacttgaaga aaagatggtg tccctgttga aagaaaaaaa
     2761 tgacttgcaa ctccaagttc aggctgaagc tgaaggcttg gctgatgctg aggaaagatg
     2821 cgaccagctg atcaaaacca aaatccagct ggaggccaaa atcaaagagg tgactgagag
     2881 agctgaggat gaggaagaga tcaacgccga gctcacggcc aagaagagga agctggagga
     2941 cgagtgctca gagctgaaga aagacatcga tgaccttgag ctgacactgg ccaaggttga
     3001 gaaggagaag cacgccacgg agaacaaggt gaaaaacctc acagaggaga tggcgggcct
     3061 ggacgaaacc atcgccaagc tgaccaagga gaagaaggcc ctgcaggagg cccaccagca
     3121 gaccctggat gacctgcagg cagaggagga caaagtcaac accctgacca aagccaaaat
     3181 caagctggaa cagcaagtgg atgatcttga agggtccttg gagcaagaaa agaagcttcg
     3241 catggaccta gaaagagcca agagaaaact tgagggtgac ttgaagttag cccaggagtc
     3301 catcatggac attgaaaatg agaaacagca gcttgatgaa aggctcaaaa agaaagagtt
     3361 tgaaatgagc aacctgcaga gcaagatcga ggatgagcag gccatcggca ttcaactgca
     3421 gaagaagatc aaggagttgc aggctcgcat tgaggagctg gaggaggaaa tcgaggcaga
     3481 gcgggcctcc agggccaaag cagagaagca gcgctctgac ctctcccggg aactggagga
     3541 gatcagcgag cggctggaag aagccggcgg ggccacttca gcccagatcg agatgaacaa
     3601 gaagagagag gccgagttcc agaaaatgcg cagggacctg gaggaggcca ccctgcagca
     3661 cgaagccaca gcagccaccc tgaggaagaa gcacgcggac agcgtggctg agctcgggga
     3721 gcagattgac aacctgcagc gggtgaagca gaagctggag aaggagaaga gcgagatgaa
     3781 gatggagatc gacgacctcg ctagtaatgt agagacagtg tctaaggcca agggaaacct
     3841 agagaagatg tgccgcaccc tggaggacca ggtgagtgag ctgaagtcga aggaggagga
     3901 acaacagcgc ctgatcaacg acctgaccac ccagagagga cgcctgcaga ccgaatccgg
     3961 tgaattttcc cggcagcttg atgagaagga agcgctggtt tctcagttat caaggggcaa
     4021 gcaagcattt acccaacaga ttgaggagct gaagaggcag ctggaagagg aagtaaaggc
     4081 caagaacgcg ctagcccacg ccctgcagtc ctcccgccat gactgtgacc tgcttcggga
     4141 acagtacgag gaggagcagg aatctaaggc cgagctgcag agggcgctgt ccaaggccaa
     4201 cagtgaggtg gcccagtgga ggaccaaata tgagacggat gccatccagc gcacggagga
     4261 gctggaggag gccaagaaaa agctggctca gcgtctgcag gcggctgagg agcacgtaga
     4321 agccgtgaac gccaagtgtg cttccctgga gaagactaag cagcggttgc agaacgaggt
     4381 ggaggacctc atgctggacg tagagaggac caacgctgcc tgtgctgccc tggacaagaa
     4441 gcagagaaac ttcgacaaga tcctggcaga gtggaagcag aagtatgagg aaacacatgc
     4501 tgagctggag gcatcccaga aggaagcccg ctccctgggt actgagctct tcaagatgaa
     4561 gaacgcctac gaggagtccc tggatcagct ggaaaccctg aagcgagaga ataagaactt
     4621 acagcaggag atttctgacc tcacggaaca gattgcagaa ggaggaaagc gcatccacga
     4681 actggagaaa ataaagaaac aagtcgaaca agagaagtgt gaacttcagg ctgctctaga
     4741 agaagcagag gcatctctgg agcacgagga gggaaagatc ctgcgcatcc agctggagct
     4801 gaaccaagtc aagtctgaga tcgacaggaa gattgctgag aaggatgagg agatcgacca
     4861 gctgaagaga aaccacattc gagtcgtgga gtccatgcaa agcacgctgg acgccgagat
     4921 caggagcagg aacgacgcca tcaggatcaa gaagaagatg gagggagatc tcaatgagat
     4981 ggaaatccag ctgaaccacg ccaaccgcat ggctgctgag gccctgagaa actacaggaa
     5041 cacccaaggc atcctcaagg acacccagct gcacctggat gacgctctcc ggggccagga
     5101 ggacctgaag gagcagctgg ccatggtgga gcgcagagcc aacctgctgc aggctgagat
     5161 cgaggagctg cgggccactc tggagcagac tgagaggagc aggaagattg cagagcagga
     5221 actgctggac gccagtgagc gcgtgcagct cctccacacc cagaacacca gcctcatcaa
     5281 caccaagaag aagctggaga cagacatttc ccagatccag ggagagatgg aggacatcgt
     5341 ccaggaagcc cgcaatgcag aagagaaggc caagaaagcc atcactgacg ccgccatgat
     5401 ggcggaggag ctgaagaagg agcaggacac cagcgcccat ctggagcgga tgaagaagaa
     5461 catggagcag acggtgaagg acctgcaact gcgtctggac gaggctgagc agctggcgct
     5521 gaagggcggc aagaagcaga tccagaaact ggaggccaga gtgcgtgaac tggagggtga
     5581 ggtagagagt gagcagaagc ggaatgttga ggctgtcaaa gggcttcgaa aacacgagag
     5641 gcgagtgaag gagcttactt accagacaga agaagaccga aaaaatatcc tcaggcttca
     5701 agatttggtg gataaactcc aagcaaaagt aaaatcttac aagagacaag ctgaggaggc
     5761 tgaggaacaa tccaacacaa atctatccaa gttccgcaag ctgcagcatg agctggagga
     5821 agccgaggag cgggctgaca tcgccgagtc ccaggtcaac aagctgcggg tgaagagccg
     5881 cgaggttcac actaaagtca taagtgaaga gtaaggcagc tctgatgctg tagaatgacc
     5941 gaagagaggc acaaaatgtg aagcctttgg tcatgtcccc atgtgattct atttaatcct
     6001 attgtaagga aataaagagc ccaagttctt tgcaagcaat a
//
