LOCUS       NM_001399313            2700 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus mitochondrial poly(A) polymerase (Mtpap),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001399313 XM_039095744
VERSION     NM_001399313.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   3  (bases 1 to 2700)
  AUTHORS   Bai,Y., Srivastava,S.K., Chang,J.H., Manley,J.L. and Tong,L.
  TITLE     Structural basis for dimerization and activity of human PAPD1, a
            noncanonical poly(A) polymerase
  JOURNAL   Mol Cell 41 (3), 311-320 (2011)
   PUBMED   21292163
REFERENCE   4  (bases 1 to 2700)
  AUTHORS   Crosby,A.H., Patel,H., Chioza,B.A., Proukakis,C., Gurtz,K.,
            Patton,M.A., Sharifi,R., Harlalka,G., Simpson,M.A., Dick,K.,
            Reed,J.A., Al-Memar,A., Chrzanowska-Lightowlers,Z.M., Cross,H.E.
            and Lightowlers,R.N.
  TITLE     Defective mitochondrial mRNA maturation is associated with spastic
            ataxia
  JOURNAL   Am J Hum Genet 87 (5), 655-660 (2010)
   PUBMED   20970105
  REMARK    GeneRIF: Our molecular investigation of an autosomal-recessive
            spastic ataxia with optic atrophy, present among the Old Order
            Amish, identified a mutation of MTPAP associated with the disease
            phenotype.
REFERENCE   5  (bases 1 to 2700)
  AUTHORS   Mullen,T.E. and Marzluff,W.F.
  TITLE     Degradation of histone mRNA requires oligouridylation followed by
            decapping and simultaneous degradation of the mRNA both 5' to 3'
            and 3' to 5'
  JOURNAL   Genes Dev 22 (1), 50-65 (2008)
   PUBMED   18172165
REFERENCE   6  (bases 1 to 2700)
  AUTHORS   Lau,N.C., Seto,A.G., Kim,J., Kuramochi-Miyagawa,S., Nakano,T.,
            Bartel,D.P. and Kingston,R.E.
  TITLE     Characterization of the piRNA complex from rat testes
  JOURNAL   Science 313 (5785), 363-367 (2006)
   PUBMED   16778019
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000017.1.
            
            On Jan 6, 2022 this sequence version replaced XM_039095744.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643299.61123.1,
                                           SRR26643298.69503.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760389,
                                           SAMEA5760434 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-176               JAXUCZ010000017.1  58036545-58036720   c
            177-349             JAXUCZ010000017.1  58032634-58032806   c
            350-583             JAXUCZ010000017.1  58031683-58031916   c
            584-808             JAXUCZ010000017.1  58030194-58030418   c
            809-1020            JAXUCZ010000017.1  58027403-58027614   c
            1021-1247           JAXUCZ010000017.1  58026647-58026873   c
            1248-1340           JAXUCZ010000017.1  58018291-58018383   c
            1341-1414           JAXUCZ010000017.1  58017953-58018026   c
            1415-2700           JAXUCZ010000017.1  58015979-58017264   c
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17q12.1"
     gene            1..2700
                     /gene="Mtpap"
                     /gene_synonym="Papd1"
                     /note="mitochondrial poly(A) polymerase"
                     /db_xref="GeneID:307050"
                     /db_xref="RGD:1310900"
     exon            1..176
                     /gene="Mtpap"
                     /gene_synonym="Papd1"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..1774
                     /gene="Mtpap"
                     /gene_synonym="Papd1"
                     /EC_number="2.7.7.19"
                     /note="isoform 1 is encoded by transcript variant 1;
                     poly(A) RNA polymerase, mitochondrial; PAP associated
                     domain containing 1"
                     /codon_start=1
                     /product="poly(A) RNA polymerase, mitochondrial isoform 1"
                     /protein_id="NP_001386242.1"
                     /db_xref="GeneID:307050"
                     /db_xref="RGD:1310900"
                     /translation="MAARGVGLLTRLPLCSQRRSRVPHSISRHLSCPGTRVASVGSEE
                     QPSGGAETGTEDKTLQKKFSEVQKERREQAQRTVLIHCPNNINEKKFLKYLSQHGSIS
                     NHFFYESFGLYAVVEFCQKDSINSLQNGTHTPTQSTEAAIPFKSRFLNLKLKNPSNQV
                     SGQSFVQADSQSPPSSKKLFELLSYAESIDAQLTALLKAFQLTEENVRLRHLTCSLIE
                     DIAAAYFPSCVVWPFGSSVNTFGKLGCDLDMFLDLDETGNLSDHKNTGNFLMEFQVKN
                     VPSERVATQKILSVIGECLDNFGPGCVGVQKILNARCPLVRFSHQGSGFQCDLTANNS
                     IALKSSELLYIYGSLDSRVRALVFGVRCWARAHSLTSSIPGAWITNFSLTMMVIFFLQ
                     RRSPPILPTLDSLKSMADAEDRCILEGNNCTFIQDINKIKPSGNTETLELLLKEFFEY
                     FGNFAFNKNSINIRQGREQNKPDSSPLYIQNPFETSLNISKNVSQNQLQKFVELARDS
                     AWILEQEKKDQPSSSSHPWGLAALLLPPGAGQTSLSRKKKKKPMSEKVKGLLASIKSN
                     SPDRSTDASGKRAVSTQA"
     exon            177..349
                     /gene="Mtpap"
                     /gene_synonym="Papd1"
                     /inference="alignment:Splign:2.1.0"
     exon            350..583
                     /gene="Mtpap"
                     /gene_synonym="Papd1"
                     /inference="alignment:Splign:2.1.0"
     exon            584..808
                     /gene="Mtpap"
                     /gene_synonym="Papd1"
                     /inference="alignment:Splign:2.1.0"
     exon            809..1020
                     /gene="Mtpap"
                     /gene_synonym="Papd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1247
                     /gene="Mtpap"
                     /gene_synonym="Papd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1340
                     /gene="Mtpap"
                     /gene_synonym="Papd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1414
                     /gene="Mtpap"
                     /gene_synonym="Papd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..2700
                     /gene="Mtpap"
                     /gene_synonym="Papd1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acttcctgct gccgtgacaa tggctgctcg cggcgtgggg ctcctgaccc gcttgcccct
       61 gtgttcccag agaaggagcc gagtcccgca ctctatttcc aggcacctca gttgtccagg
      121 aaccagagtc gcgagcgtcg ggagcgaaga acagccttcg gggggtgcgg aaacaggcac
      181 tgaagacaag accctccaaa agaaattctc tgaggtgcag aaagagagaa gagaacaggc
      241 ccagcggact gttttgatac attgtccaaa taatatcaat gagaaaaagt ttctcaagta
      301 tctgtcccag catggatcca tcagtaatca tttcttctac gagagctttg gtctctatgc
      361 tgttgtagaa ttttgccaaa aagacagtat aaactctctg cagaatggaa cacatactcc
      421 aacccagagc acagaggctg caattccatt taagtcacgt ttcctgaact taaagctgaa
      481 gaatccatct aaccaagtat ctggacaatc gtttgtacaa gcagacagcc agtcacctcc
      541 ttcaagcaag aaactctttg aattgctgag ttacgcagaa agcatagacg cccagctgac
      601 cgctcttctg aaggccttcc agctcacgga ggagaatgtc aggctccgcc atctcacctg
      661 ctctctgatc gaggacatcg ccgctgccta ttttcccagc tgtgtcgtct ggccctttgg
      721 ctcctcagtg aacacgtttg gcaaattagg atgtgatttg gacatgtttt tagatttaga
      781 cgaaactgga aatctcagtg atcacaagaa tacagggaat tttttaatgg aatttcaagt
      841 gaaaaacgtt ccctctgaaa gggttgcgac ccagaagatc ttatctgtaa taggagagtg
      901 ccttgacaat tttggccctg gctgtgtggg tgttcagaag atactcaatg ctcgctgtcc
      961 actggtgaga ttctcccatc agggctctgg attccagtgt gacctgactg ccaacaatag
     1021 tattgctttg aaaagttccg agctccttta tatatatggc tccctggatt caagagtaag
     1081 agctttggtg ttcggtgtgc gatgctgggc tcgggcacat tcgttaacaa gcagtattcc
     1141 tggtgcttgg attacaaact tctccctaac aatgatggtc atctttttcc tccagagaag
     1201 atcacccccc attctcccaa ctctggattc tctaaagtcc atggcagatg ctgaagacag
     1261 atgtattcta gagggaaaca attgtacatt catccaggac ataaataaaa tcaaaccttc
     1321 tggaaacaca gaaacactag aattactact aaaggaattt tttgagtatt ttggcaattt
     1381 tgctttcaat aagaattcca taaacatccg acagggaagg gagcaaaaca agcctgattc
     1441 ttctcctctg tacatccaga atccttttga aacatctctt aacataagca aaaatgtgag
     1501 tcagaaccag ctacaaaaat ttgtggaatt ggccagagac agtgcctgga ttttggaaca
     1561 ggaaaaaaag gatcagcctt cttccagtag ccatccctgg gggctggctg ccctcctgct
     1621 gcctcctggg gcaggccaga catctctctc tcggaaaaag aagaaaaagc cgatgagtga
     1681 aaaagtcaaa ggcttgttag catccataaa aagtaacagc cctgaccgtt ctacagatgc
     1741 cagtggaaag agagccgtca gtacacaggc gtgatggtag ccctttggac ttctgggaag
     1801 aactgccacc aaatgttagc aggccttaac gtttatatat ctgacgtcat caccctttta
     1861 cttggtcaga agttctggat attttcttcc aatcaggtgg agtttattaa aatatcagtg
     1921 gcaaaaaaaa ttgcaaaaga catatttgaa ctctgcaaag tgaatgataa aatgaatttt
     1981 tatcatcaac ttggttgata gttctgtcag tttctagttg gaaatcattt caaacccctt
     2041 tcagttatca tcatatatag gtaatgaatg caataaagct tcaaaatttg tatttttagt
     2101 atctattttc aatcaaagtt ctcttaggtg ttttgtcttc tgaatgaata aatatatata
     2161 tcatatatag cctgctgggg atatatcata tatatatata aataaaataa agcagtgggc
     2221 ttgtgtccca tcaggttcct taggcagacc ttgtctttgt tgatttcctg ctcttcacac
     2281 accccctccc acttttctcc cccactaacc ctcagttgga ttttgcttca tgtatgagtg
     2341 actgttctgc tactctgtcc cttcccctcc ccataaggtc ctgctcccaa aggtccccag
     2401 cgttttatga cctgtacctc atacatgctt atgcttcacg taatatcaaa catgaaccca
     2461 aggttcacac tggagaggaa aggaacagct ttttcctcct ggatctggtg gacttcaagc
     2521 aaagttcccc cattcccagt taaacaggtt gggttggatt tcccaaactg tgtctctaac
     2581 acataaaaat gcagtcattg tgttctgtgt tctggtcaga tatgtagaat tgtggatata
     2641 ttgtactttc aactcaaatt ttatttttgt tttcaagaat aaaaccctgt ttctactgaa
//
