LOCUS       XM_342975               7278 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus methylenetetrahydrofolate reductase
            (Mthfr), transcript variant X1, mRNA.
ACCESSION   XM_342975
VERSION     XM_342975.8
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051340.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_342975.9.
            
            On Jan 21, 2021 this sequence version replaced XM_342975.7.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..7278
                     /gene="Mthfr"
                     /note="methylenetetrahydrofolate reductase; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 48 ESTs, 6 Proteins, and 100% coverage of the
                     annotated genomic feature by RNAseq alignments, including
                     105 samples with support for all annotated introns"
                     /db_xref="GeneID:362657"
                     /db_xref="RGD:1309952"
     CDS             1539..3626
                     /gene="Mthfr"
                     /codon_start=1
                     /product="methylenetetrahydrofolate reductase (NADPH)
                     isoform X1"
                     /protein_id="XP_342976.3"
                     /db_xref="GeneID:362657"
                     /db_xref="RGD:1309952"
                     /translation="MNHQKAGVLPDGHCYTSLGMWASEAGCVGLSVPPSISRNPAMSN
                     EARRSGSPSPRSEGSSSGSESSKDSSRCSTPSLDPDRHERLREKMRRRMDSGDKWFSL
                     EFFPPRTAEGAVNLISRFDRMAAGGPLFVDVTWHPAGDPGSDKETSSMMIASTALNYC
                     GLETILHMTCCQQRQEEISGHLHRAKQLGLKNIMALRGDPVGDHWEAEEGGFSYATDL
                     VKHIRNEFGDYFDICVAGYPKGHPDAESFEDDLKHLKEKVSAGADFIITQLFFEASSF
                     FSFVKACEDIGISCPILPGIFPIQGYTSLRQLVKLSKLEVPQTIKDVIEPIKDNDAAI
                     RNYGIELAVNLCRELLDSGLVPGLHFYTLNREVATMEVLKQLGMWTEDPRRPLPWAVS
                     AHPKRREEDVRPIFWASRPKSYIYRTQGWDEFPNGRWGNSSSPAFGELKDYYLFYLKS
                     KSPREELLKMWGEELTSEESVFEVFEHYLSGEPNRHGYRVTCLPWNDEPLAAETSLMK
                     EELLRVNRLGILTINSQPNINGKPSSDPVVGWGPSGGYVFQKAYLEFFTSRETVEALL
                     QVLKTYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIVQPTVVDPISFMFWK
                     DEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFLLDNCLWQVVEDTFEL
                     LNRHTTKKEAQAP"
ORIGIN      
        1 ttcgagacag tttctctgtg tagccctagc tgttctggaa ctctctgtat atcaggcctc
       61 cgttgcctcc tgagagctgg ggaacaatgg gtcattagct tcagtgccca ggtgcccata
      121 gcgtcctgct gttcacatac atctatcaca gtgcttctgg gagtggtggg cactcagtgt
      181 ttgccacagt cttaaatgat cagtaggtgg cagaggggca ttttgagact gaggtcttgg
      241 cagtttgggg cccaggcact ggggatgcca cgcttgagac tggtctctgg tcccgatccc
      301 aggcagggga ccggacagta gaagggtaaa aaaatttgtc agttcacagt ctcaaccttg
      361 tatgttatgg gagctcaact aatagtggat agccggtggg tgctggttga tgtgtggaac
      421 atattagaaa tgcttaaatt aaaccttgga gcagtttgct gtgcctgagt cagcaactcc
      481 ggttttgttc cagcagtgaa aatgaagcat cttctttggg tggaggagga aggtcttggc
      541 tgatatgaaa ggttaccgtc tttctcaccc tttgtgagaa cctggcagtt ttaggttaga
      601 acagaagtgg cacagataga ctgggaataa cactggcact aagtctggcg tctgggaggg
      661 gaaggagcaa tggatttcat ccgattcttg aggctgagac ccaaaatagt acattgactt
      721 ggaggtggaa gtttctggaa agaaacaaac ctgaccgttc ctctgttgct ccacgtcacc
      781 atacttgctc cacgtcacca tactgatagc cactaccgtg tttgcccatc actgtgctgc
      841 agccgtagtg tttgttttcc tttttggcgt ttgtcttttc caggctagac tgcagtatgg
      901 ccctatgccc tgttagccag gctgtgattg ccgtgaccgg gagggtgcat atgtgcactg
      961 caaaggtgca gtgactgtgg tcactgatcc ttttgactta attggcgtgt ttagggagag
     1021 cggctagcag ggcagacaca gataggacaa cctttgttca gagttggaca taaagcctta
     1081 cccttggtga cttcagtcca cctgcttctg catcccaggc ccgttcaagt tctagctcac
     1141 gacaaacaca acagtctttt tgaagtagtg tgatctcagg agatcaggct gcctagccag
     1201 gctgcagaaa acctgtaaca cgttccaggc ggggtcagcc acagcgagct gttctttctt
     1261 tggaggggga aacctagtga tcctgatagg ctaatagcct aatctccact aacccctgca
     1321 gtccctgcct gcccactcct cccctcccct cccccattca tcttccttct ttgacttgcg
     1381 cctgagcctt ctccagcctg gaaaccttac tcccgccccc tttcgtggcc ccagcttcag
     1441 ctcagggctc tttgtcacag ctccgcccac ttaggcacac cctctaggaa aggttcctcc
     1501 gttccctcgc cactcactct gggtctaagc agatcgggat gaaccatcaa aaagccgggg
     1561 tcctccccga tgggcactgt tacacatccc tggggatgtg ggcctcagag gctggctgcg
     1621 tgggactttc tgtgccaccc tctatcagca ggaatccagc catgtcgaac gaggccagaa
     1681 gaagtggcag ccccagcccc cgatctgagg gcagcagcag tggcagcgag agttccaagg
     1741 acagttccag atgttccacc cccagcctgg acccggaccg gcacgagaga ctgcgggaga
     1801 agatgagacg cagaatggac tctggtgaca agtggttctc cctggagttc ttccctcctc
     1861 ggacagctga gggagctgtt aacctcatct cgaggtttga ccggatggca gcagggggcc
     1921 ccctcttcgt ggatgtcacc tggcacccag ctggagaccc tggctcagac aaggagacct
     1981 catctatgat gattgccagc acagcactga actactgtgg cttggaaacc atcctgcaca
     2041 tgacctgctg ccagcagcgc caggaggaga tctcaggcca cctgcacaga gccaagcagc
     2101 tcggcctgaa gaatataatg gcgctgaggg gagaccctgt aggtgaccac tgggaagcag
     2161 aggaaggagg cttcagctat gccacagacc tggtgaagca catccggaac gagtttggag
     2221 actattttga catctgtgtg gcaggttacc ccaaaggcca cccggatgca gagagcttcg
     2281 aggatgacct gaagcacctg aaggagaagg tatcagcagg cgccgacttt atcatcactc
     2341 agctcttctt tgaggccagc tccttcttca gcttcgtgaa ggcctgcgaa gacataggca
     2401 tctcctgccc tatcctgcct gggatcttcc ctatccaggg ctacacctcc cttcggcagc
     2461 ttgtaaaact gtccaagctg gaggtaccac agacgatcaa ggatgtgatc gagccgatca
     2521 aagacaacga tgctgccatc cgcaactacg gcattgagct ggctgtgaac ttgtgccggg
     2581 aactgctgga cagtggcttg gtgccgggcc tccacttcta cactctcaac cgcgaggtgg
     2641 ccaccatgga agtgctgaag caactgggca tgtggaccga ggaccccagg cgtcccctgc
     2701 cctgggctgt cagtgcgcac cccaagcgca gggaggaaga tgtgcgtccc atcttctggg
     2761 cctccagacc aaaaagctac atctaccgca cacagggctg ggatgagttt cccaatggcc
     2821 gctggggtaa ttcttcctca ccagccttcg gggagctgaa agactactac ctcttctacc
     2881 tgaaaagcaa gtcccccagg gaggagctgc tcaagatgtg gggtgaggag ctcaccagcg
     2941 aagagagtgt ctttgaggtc tttgagcact acctctcagg agagccgaat cgccatggct
     3001 acagagttac ctgcctgccc tggaacgatg agcccctggc ggcggaaacc agcctgatga
     3061 aggaggagct gctccgcgtg aacaggctgg gcatcctcac catcaactcc cagcccaaca
     3121 tcaacggaaa gccgtcctca gaccctgttg taggctgggg ccccagtggg ggctatgtct
     3181 tccagaaggc ctacctcgag ttcttcacct cccgagaaac cgtggaggct cttctgcaag
     3241 tgttgaagac gtatgagttg cgagtcaatt accacatcgt ggacgtgaag ggagagaaca
     3301 tcactaatgc ccctgagctg cagcccaacg ctgtcacgtg gggcatcttc cctggccgtg
     3361 agatcgtcca gcccactgtg gtggatccca tcagcttcat gttctggaag gatgaggcct
     3421 ttgccctgtg gatcgagcag tggggcaagt tatacgagga ggagtccccg tcccgcatga
     3481 tcatccagta catccacgac aactacttcc tggtcaacct ggtggacaac gagttcttgc
     3541 tggacaactg cctgtggcag gtggtagagg acacgtttga gcttctcaac aggcacacca
     3601 cgaagaaaga ggcacaggct ccgtgaggct gcgtccggta tcgtcctctc ccggaggccc
     3661 ctctctccct catctctcta cacatggtat tggcagctag actggcagga agcatccagg
     3721 ctctggctgg acctcagatg tcccatcgag gaggctgatg ctctctgctc aagcccagag
     3781 cccatgttct tgcagtggca agggccccca tcctcaacgg gaggttgcca agcaccaagg
     3841 cagcctctag ggggtgacct ttgttctggg gtctatgtga gacaggcaga ggcacttgta
     3901 ctaaaggagg taggaggaga tgtgccaagg tgggtacaga aacctccccc tgctcagcgg
     3961 accaggccaa agagtgcagg aggtctgtct cttccatggg gctcagaagg ttgtctagtc
     4021 tgtggctggg aagaaagtgc cccttaaggg ttaaaggcat tctgtcctca gtggaggcat
     4081 cctcttggcc ttgttttatg tcctcttaat gagattcacc tgggcagggc tcccccactc
     4141 tgcctgtggc ggtgtgcagc ccactccctg ccttctgagc agactcctgc cagctgctgc
     4201 cttctctgtt tggcctagac taggagctgt ccctggcact cctccccagt cagccttcag
     4261 gcctcgctgc ctggggcagt gactggcagg tacagatggc tgcttctgag gcggggctcc
     4321 cagcctgctg gtcttttgag tcagggaagg ggaggaggct gtccttaggt agagcagggg
     4381 ctcgaccctc cctccccctg tctcccaggc ctacacaact gcagtgctga gccgactcct
     4441 ccaactgtgg gcctgttccc tccctcggaa ggccaggcct ggttctgctt attcctcagg
     4501 aacatttcat agctgctgtt gggccctact caccgcacac ctgtgccgtc tggcccggcc
     4561 actgccaact gtctccaggg gcacaggctt gtggactctc tggtgctgct tttcagaggc
     4621 ctggaagctg ggcagaacaa gaaatgccac ttgactctgg ctcccccagg gcctgtactg
     4681 ggcctgtcac ctcagccacg ttctgtgtgt ccctggcagc cagcccaccc tgtctctacc
     4741 caaaaatcca ggctcagaat agactggact tgtgacctgg gaagctatgc ccagtgacct
     4801 gccaataaga ctcgtgtgct gggggttggg gatttagctc agtggtagag cgcttgccta
     4861 ggaagcgcaa ggccctgggt tcagtcccca gctccgaaaa aaagaaccaa aaaaaaaaaa
     4921 agtaagactc gtgtgctgga cctgggccta cagggaccag catggtgagc caccgccaca
     4981 aacagcctgg ttctcagact gagtgaaatt gacactgggc agggctgaag gccatagtca
     5041 agggctctca tgtcctgtac tctgtaacca gtagccaact caactgcatg aggaaacccc
     5101 acagtggtga gcagggacac cccacccctc ccaggacagc ctgcaggtga cactggtttt
     5161 ggaagactgt ctgggtgaca cgagataaag tcacctggag aaagacacag ctgccttctc
     5221 tttggagtag cagctgttga gccacccaga actgaaggac cttttcccac catgtcctct
     5281 acgccagttc tggctgccat tgtctaagct acagagagtg gatagaaaag accctctacc
     5341 atcctgtcag cctggccctc tactgccact ccaggggcca tcgctaagaa ccgttaacag
     5401 atgaggaggc ctggcccatc cctgaggctt tgggcatgag atgcagttag cgtctccagt
     5461 tagccaaggt taggtacaat gaatgggaag ttgctcccag ggccgctgct tggtagagag
     5521 gaactgagcc taccgacctg accaagacag ctgctccacc cagtgaacat ccacccagcc
     5581 ctgccatgcc cttgccaggc tccaggcctg ctgggaaccc agcgagggta aagagcaaga
     5641 gccccagggc cttcctgcca gagttgaaag ccagtggcct gagtggactg accacccctc
     5701 cccatgcaca ggatcgtgac cctgccccag tcacctttga agagccttgc cttggccctc
     5761 caaggatctg atctcagagc tacagagctc acctcatcct gtagggggcc atcggagggc
     5821 ctatgcaggc agtggctgcc agaactggag ggttgggcag aggggtttct ctgtgtcacc
     5881 tggaaaggag aattgggtca ggagaggatg atgccttgat ttaggagaac agaagctccc
     5941 ctcaagtctc caggctgtgg gctggcccaa gggactaata ggaaaagaag ggacttctcc
     6001 aaacagctgg ggcattcgag aagagatgga cagggcattg tttcacaggg tttgacctga
     6061 gggctgagac ccaaggaccc agcttcttat tcaaagccca ggatgggtct tctgtaggcc
     6121 tcttccacac gcccatgtca ggctgggcag cacattcttt acaacaggtg accagtagct
     6181 gtgagtctga aggatcaact gcagtgggct tgagcctact caccaagagg gagtcctgcc
     6241 tgcctcctgc cagagcaggc tcccacccgt gtgcataatg gggtgcttca cctgatactg
     6301 aggagtccca agacccccat atgtggcact actctccctg actccccctc ccccagttga
     6361 ctgaaggcat taagcagagg ctggcccagt aagagccgcc ctgtggtgga gctcaggcca
     6421 gccctgagac tgatgttgtg ctcctgagtt aagctttagg atgtcgctga agccttgcct
     6481 ctcacatagg gcctgacccc aaagtctggg ctcccaacta atgcaacaga cggaggggac
     6541 tcccagccgc cacagaatgg ctcaccacct gcacatgttg ccagggagag gttacctggg
     6601 cgtgtgtgtg cctgcgatga agagcaggac cctgggtacc attctcagtg tctgctgtgt
     6661 ttctagaaac ctcagtttat cagcgtatca gccttgcctt cactaagaag aagagagtga
     6721 aagtatatat actgttaaaa ggtggtagtg tactagtgaa attttcacaa ggttgaggca
     6781 ggaggaccag aggctgactc atgagcttga agccacctgg gctatacaag atgggtcaaa
     6841 gcctggaaca tcaagtcaat ccacaagtct acagggggat tctgaaaccc agcaatgcta
     6901 gtcttgcgat tccatttcaa aggcaaagcc actgtgatga gtttttatgc tctttccaaa
     6961 gtctgttcct tctggcagtc tgaagcaaga ctcttggtga cgccaaattg tccttaggaa
     7021 cactgtgtag cctgtcagga tctcgggtcc tctggttacg tctccagacg tccaggtggt
     7081 actgtttcct gcacctggca ttgtgattct cttccctcct ataggccatc agcctctcac
     7141 tgttgtgtca aagctaagga cttccggtct gaaggtgaca tttttgtaca aaaaaagcca
     7201 aaacaaaacc ctactttcta ctttattttc tgtaatgagc acatttcatt tttccaaaat
     7261 aaaaaattaa aaaataaa
//
