LOCUS       NM_001109398            2061 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Rattus norvegicus methylenetetrahydrofolate dehydrogenase (NADP+
            dependent) 2, methenyltetrahydrofolate cyclohydrolase (Mthfd2),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001109398 XM_001056154 XM_001056213
VERSION     NM_001109398.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Hu,W.
  TITLE     EP300-mediated H3 acetylation elevates MTHFD2 expression to reduce
            mitochondrial dysfunction in lipopolysaccharide-induced tubular
            epithelial cells
  JOURNAL   Ren Fail 46 (2), 2369342 (2024)
   PUBMED   39230047
  REMARK    GeneRIF: EP300-mediated H3 acetylation elevates MTHFD2 expression
            to reduce mitochondrial dysfunction in lipopolysaccharide-induced
            tubular epithelial cells.
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Bohm,D., Keller,K., Pieter,J., Boehm,N., Wolters,D., Siggelkow,W.,
            Lebrecht,A., Schmidt,M., Kolbl,H., Pfeiffer,N. and Grus,F.H.
  TITLE     Comparison of tear protein levels in breast cancer patients and
            healthy controls using a de novo proteomic approach
  JOURNAL   Oncol Rep 28 (2), 429-438 (2012)
   PUBMED   22664934
REFERENCE   3  (bases 1 to 2061)
  AUTHORS   Watkins,D. and Rosenblatt,D.S.
  TITLE     Update and new concepts in vitamin responsive disorders of folate
            transport and metabolism
  JOURNAL   J Inherit Metab Dis 35 (4), 665-670 (2012)
   PUBMED   22108709
  REMARK    Review article
REFERENCE   4  (bases 1 to 2061)
  AUTHORS   Tibbetts,A.S. and Appling,D.R.
  TITLE     Compartmentalization of Mammalian folate-mediated one-carbon
            metabolism
  JOURNAL   Annu Rev Nutr 30, 57-81 (2010)
   PUBMED   20645850
  REMARK    Review article
REFERENCE   5  (bases 1 to 2061)
  AUTHORS   Ito,Y.N., Ito,M., Takita,H., Yoneya,S., Peyman,G.A., Gehlbach,P.L.
            and Mori,K.
  TITLE     Transpupillary thermotherapy-induced modification of angiogenesis-
            and coagulation-related gene expression in the rat posterior fundus
  JOURNAL   Mol Vis 12, 802-810 (2006)
   PUBMED   16885923
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2061)
  AUTHORS   Christensen,K.E., Mirza,I.A., Berghuis,A.M. and Mackenzie,R.E.
  TITLE     Magnesium and phosphate ions enable NAD binding to
            methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate
            cyclohydrolase
  JOURNAL   J Biol Chem 280 (40), 34316-34323 (2005)
   PUBMED   16100107
REFERENCE   7  (bases 1 to 2061)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   8  (bases 1 to 2061)
  AUTHORS   Pawelek,P.D. and MacKenzie,R.E.
  TITLE     Methenyltetrahydrofolate cyclohydrolase is rate limiting for the
            enzymatic conversion of 10-formyltetrahydrofolate to
            5,10-methylenetetrahydrofolate in bifunctional
            dehydrogenase-cyclohydrolase enzymes
  JOURNAL   Biochemistry 37 (4), 1109-1115 (1998)
   PUBMED   9454603
REFERENCE   9  (bases 1 to 2061)
  AUTHORS   Yang,X.M. and MacKenzie,R.E.
  TITLE     NAD-dependent methylenetetrahydrofolate
            dehydrogenase-methenyltetrahydrofolate cyclohydrolase is the
            mammalian homolog of the mitochondrial enzyme encoded by the yeast
            MIS1 gene
  JOURNAL   Biochemistry 32 (41), 11118-11123 (1993)
   PUBMED   8218174
REFERENCE   10 (bases 1 to 2061)
  AUTHORS   Rozen,R., Hum,D.W. and Mackenzie,R.E.
  TITLE     In vitro synthesis of the trifunctional protein,
            methylenetetrahydrofolate dehydrogenase--methenyltetrahydrofolate
            cyclohydrolase--formyltetrahydrofolate synthetase, in normal and
            transformed cells
  JOURNAL   Can J Biochem Cell Biol 63 (11), 1189-1193 (1985)
   PUBMED   3878730
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473957.1.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_001056154.1, XM_001056213.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN16093045
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q34"
     gene            1..2061
                     /gene="Mthfd2"
                     /note="methylenetetrahydrofolate dehydrogenase (NADP+
                     dependent) 2, methenyltetrahydrofolate cyclohydrolase"
                     /db_xref="GeneID:680308"
                     /db_xref="RGD:1311313"
     exon            1..173
                     /gene="Mthfd2"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..1125
                     /gene="Mthfd2"
                     /note="methylenetetrahydrofolate dehydrogenase (NAD+
                     dependent), methenyltetrahydrofolate cyclohydrolase;
                     methylenetetrahydrofolate dehydrogenase 2"
                     /codon_start=1
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/cyclohydrolase, mitochondrial"
                     /protein_id="NP_001102868.1"
                     /db_xref="GeneID:680308"
                     /db_xref="RGD:1311313"
                     /translation="MVSVSLSALAVRLLRPTHGCHSRLQSFHLAAVRNEAVVISGRKL
                     AQQIKQEVRQEVEEWVASGNKRPHLSVILVGDNPASHSYVLNKTRAAAEVGINSETIV
                     KPASVSEEELLNSIRTLNDDENVDGLLVQLPLPEHIDERKICNAVSPDKDVDGFHVIN
                     VGRMCLDQYSMLPATPWGVWEILKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAH
                     ERPGGDATVTISHRHTPKEQLKKHTILADIVISAAGIPNLITADMIKEGATVIDVGIN
                     RVQDPVTAKPKLVGDVDFEGVKKKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRPE
                     ELEVLKSTQRGVATN"
     exon            174..358
                     /gene="Mthfd2"
                     /inference="alignment:Splign:2.1.0"
     exon            359..481
                     /gene="Mthfd2"
                     /inference="alignment:Splign:2.1.0"
     exon            482..634
                     /gene="Mthfd2"
                     /inference="alignment:Splign:2.1.0"
     exon            635..742
                     /gene="Mthfd2"
                     /inference="alignment:Splign:2.1.0"
     exon            743..835
                     /gene="Mthfd2"
                     /inference="alignment:Splign:2.1.0"
     exon            836..961
                     /gene="Mthfd2"
                     /inference="alignment:Splign:2.1.0"
     exon            962..2061
                     /gene="Mthfd2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggcttctgtg ggcccggggt atagcttcgt ggcccgcgcg cgcgcttctc tccggcgcga
       61 gcaccggcgc gggccatggt ttctgtttcc ttgtctgcgc tggcagtgcg gttgttgcgc
      121 cccacgcacg gctgccactc ccgcctacag tccttccacc tggcggcagt acgaaatgaa
      181 gccgtggtca tttctggaag gaagctggcc cagcagatta agcaagaagt acggcaggag
      241 gtggaagagt gggtggcctc gggcaacaag cggccacacc tcagtgtcat tctggttggc
      301 gacaatcctg ccagtcactc ctatgttctc aacaaaacca gggcagcagc tgaagtggga
      361 atcaacagtg agacaattgt gaaaccagcc tcagtgtcag aggaagagct gttgaattca
      421 atcaggacat tgaatgacga tgagaatgta gatggcctcc ttgttcagct gccactccca
      481 gagcacattg acgagagaaa gatctgcaat gctgtttctc ctgacaagga tgttgatggc
      541 tttcatgtca tcaacgttgg gcgcatgtgt ttggaccagt attccatgct gccagcaact
      601 ccatggggcg tgtgggagat actcaagcga acaggcattc caaccttagg gaagaatgtg
      661 gtagtggctg gcaggtccaa aaacgttggg atgccaattg caatgctgct gcacacggat
      721 ggggcacacg agcggcctgg gggtgatgcc acagtcacaa tatctcatcg gcacactccc
      781 aaagaacagc tgaagaagca cacgatcctt gcggacattg tgatatctgc tgctggcatt
      841 ccaaacctca tcacagctga catgatcaag gaaggagcaa cagtcatcga cgtggggata
      901 aacagagttc aagatcctgt caccgcaaag cccaagttgg ttggagatgt ggattttgaa
      961 ggagtcaaga agaaagctgg ttacatcact cctgtccctg ggggtgttgg tcccatgaca
     1021 gtggccatgc tcatgaagaa caccattatt gctgcaaaga aagtgctcag gccagaggag
     1081 ctggaagtgt tgaagtccac acagcgcgga gttgccacca actagccgcg agcgagccag
     1141 tctgggaggc aaagcgggcc aagagggatg cagtgttctt aatttattct gttgaaatgg
     1201 tttaaaatgt ttgtatttat tgaaagctta gacaggtggc tgtgtgtgca cacatggctc
     1261 tgcagaacct caccagggtg cccatccgtg tcctgccttt gacctggtga tgcctgggag
     1321 acgcctcaca ctcagtggat gctgaactcc atcaaaaaga gaaaaaaaat ctgccttttt
     1381 gggctctcat ttcccaagtg ctattccaat acaagttgat ggctcatctt aggtggcaag
     1441 cctctgagtt caactttcaa accaaaggaa aacttagtag agaaaattaa gggaaagcaa
     1501 gagacagcag actgtgtctc atcggcactc ccttccttcc tgtgcatctg ggagaaccca
     1561 ggttgccctg aggacagctc agctggctgg gctggtaaac gaaagcccac tgttactagc
     1621 tgtgtcccca tggcgggtca tcagggaagg ttgagtctcc ccactcgctg ttacccttct
     1681 gtgagcttgc cgtgttttaa ttttctagaa tcaaaaggct cattttataa atgatgtatg
     1741 tattatcatg tttggctttt gcagcacatt agagagagtg tgtgtgtgtg ttttctttgg
     1801 tgtatgcttt gaggcctttt ttttttttaa taataaaact ctggtacagt aatcaggagg
     1861 gcagcttgtg ccaccaaaga actgtcgaca gctgcctgca tgtctttcac ggaccctggc
     1921 tttcctgaaa gttctcaagg agcttgagtt aatgagtcag attttcttat taaagggttt
     1981 tgttgctgtt gttgggtttt gtttttttgt tttgttttgt ttttgctttg tttttaaata
     2041 aagcacatct gtctggtgtg g
//
