LOCUS       XM_039081224            4763 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus methylenetetrahydrofolate
            dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), transcript
            variant X1, mRNA.
ACCESSION   XM_039081224
VERSION     XM_039081224.1
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051336.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..4763
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /note="methylenetetrahydrofolate dehydrogenase (NADP+
                     dependent) 1-like; Derived by automated computational
                     analysis using gene prediction method: Gnomon. Supporting
                     evidence includes similarity to: 6 ESTs, 2 Proteins, and
                     100% coverage of the annotated genomic feature by RNAseq
                     alignments, including 61 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:361472"
                     /db_xref="RGD:1307311"
     CDS             188..2113
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /codon_start=1
                     /product="monofunctional C1-tetrahydrofolate synthase,
                     mitochondrial isoform X1"
                     /protein_id="XP_038937152.1"
                     /db_xref="GeneID:361472"
                     /db_xref="RGD:1307311"
                     /translation="MSARLPLLLRQLGHQQLPPGPACRLRELCRSGSRSSSSGGGDPE
                     GLRGRRLQDGQAFISHSPGSPEDPGMDSIVRDVIHNSKEVLNLLQEKNPAFKPVLAVI
                     QAGDDHLMQNVNQNLAKEAGLDITHICLPPDSGEDEIIDEILKINEDPRVHGLALQIS
                     EDSLCNKVLNALKPEKDVDGVTDVNLGKLVRGDAPECFVSPLARAAVELIEKSGITLD
                     GKKVLVIGAHGPLEAALQCLFQRKGSMTMSSPWITPQLQDKLREADIVVLGSPKPDEI
                     PAAWIPSGATMLNCFHDFLSGKLRGGSPGVPMNRLIADESVSLLAAALRVQNMVNSGR
                     RWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGK
                     SKAKVRLSLLERLKDQTDGKYVLVAGITPTPLGEGKSTVTIGLVQALTAHLNVNSFAC
                     LRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAAAIDTRIFH
                     ESTQTDKALYNRLVPLVNGVRKFSEIQLSRLKKLGIHKTDPTALTEEEVRKFTRLNID
                     PSTITWQRVLDTNDRFLRKITIGQGSTEKGYSRQHQLPGGLGEIHSASCRRWIMEASA
                     GRGNSSADMEDMENFRE"
ORIGIN      
        1 ctaggtacag tgccccctag gggcctcagt tcctcccacg aggaagagcc tggtcgttcc
       61 tgctgctggt cctttactgt gccgcttggt tctctctctc tactgccttc ctcgccaacc
      121 aggggaggaa cttgcagccg catctcggtg cctccggagg accagtcaca ccgcgctctg
      181 cagcggcatg agtgcccgcc ttcctctcct gctacgccaa ctcggccacc agcagctgcc
      241 cccgggcccg gcgtgtcgcc tccgtgagct gtgtcgatcc ggcagcagaa gcagcagcag
      301 cggaggtggt gaccccgagg ggttgcgcgg gcggcggctg caggatggcc aagccttcat
      361 cagccatagc ccaggcagtc cggaagaccc cgggatggac tcaatcgtta gagacgtcat
      421 tcacaattcc aaagaagtgc tgaacttgtt gcaagaaaaa aaccctgcct ttaagccagt
      481 tcttgctgtg attcaggcag gagatgacca cctgatgcag aatgtgaacc agaatttggc
      541 caaggaggct ggcctggaca tcactcacat ctgcctacct ccagacagtg gagaggatga
      601 gatcatagat gaaatcctga agattaatga agaccctaga gtgcatggcc ttgcccttca
      661 gatctctgaa gattcattgt gcaacaaagt tctcaatgct ttgaaaccgg aaaaagatgt
      721 ggacggggtg accgatgtca atctagggaa gctggtgcga ggtgacgctc ctgaatgttt
      781 tgtttcgccc ttggccagag ctgcagtaga actcattgag aaatcaggga tcaccttgga
      841 tggaaagaag gtcttggtca taggggctca cgggcctctg gaagctgccc tgcaatgtct
      901 tttccagaga aaaggatcca tgacaatgag ctccccgtgg ataacacctc agcttcaaga
      961 caagcttcga gaggcagaca ttgtggtctt gggctcacct aagcccgacg aaattcctgc
     1021 tgcttggatc ccgtcaggag ccaccatgct caattgcttc catgacttct tgtcagggaa
     1081 gctgagagga ggttctcctg gggtccccat gaacagactc attgccgacg agagcgtgag
     1141 tctccttgct gcagctctgc gggttcagaa catggtgaac agtggcagga gatggctccg
     1201 agagcagcag catagaagat ggcgactcca ctgcttgaaa ctgcagcctc tctcccctgt
     1261 gcccagtgat attgagatct cacgaggaca gactccaaaa gcggtggatg tccttgccaa
     1321 ggagatagga ttgcttgcag atgaaattga aatctacggc aagagcaagg ccaaagtccg
     1381 cttgtccctg ctggagaggt taaaggatca aacagatgga aaatacgtct tggttgctgg
     1441 gatcacaccc accccacttg gagaagggaa gagcacagtc accatcggac tagtgcaggc
     1501 actaactgct cacctgaacg tcaactcctt cgcctgtctg agacagcctt cccagggacc
     1561 cacttttgga gtgaaaggag gagctgcagg tggtggatat gctcaggtca ttcccatgga
     1621 ggagttcaac cttcacctga ctggggacat ccacgccatc actgccgcta ataacttact
     1681 ggctgctgcc atcgacacga gaattttcca tgagagcact cagacagaca aggctctata
     1741 caatcgactg gttcccttag tgaatggtgt cagaaaattt tcagaaattc aactttctcg
     1801 gctgaagaaa ctggggatac ataagactga tcccaccgct ctgacagaag aggaagtgag
     1861 gaagttcacc cgcctcaaca ttgacccctc taccatcaca tggcagagag tattggacac
     1921 gaatgaccga tttctgagaa aaataactat tgggcaggga agcacggaga aaggatattc
     1981 ccggcagcac caactgccag gtggccttgg ggaaattcat tcagcatcct gcaggagatg
     2041 gataatggaa gcttcggcag gaagaggaaa cagcagcgct gatatggaag acatggagaa
     2101 cttcagagaa taggggacac tcctcctgga ccaagacggc cattgacggt cactatagag
     2161 aggggctgtt gctgacaaaa ggcagagact atgcaggaaa catcactttt gatgtgtcta
     2221 aacattgagc ataaaacaaa cattaaggct agtggtcatc tgggacatgt ggcagttatt
     2281 gtacctaaaa taaggacatt caacaagtaa tactcagaca gggctcgtcc cctggccatc
     2341 ttatatcaca ccaaagggtc ctatccccaa actcgcctct cttccccaac taagtcctgc
     2401 acctgaatcc cttcctatca tgctggattt tgtcccctcc ttaaagcaga gttttgggtt
     2461 ctgtattcta tacttacggc aaacatttga cacagtaggt ggttggtgtc agtagattgt
     2521 tgaacaaata aaacaaatga cagttgacag atatgaaaat aaaataacac tgtaaaatgg
     2581 tatctatatt gctacaaagt aagacagtcg tgggagctac ctgtggtgcg tgcctgtaat
     2641 cccagcatta ggcggcagaa gcagatggat ccctgtgttt gaggacagcc tagtatacat
     2701 agtacaagtt gagattactt tgccaggctg cttatatttt gtattgcgta aagtaatgaa
     2761 gtgttatatt agggtgttgg ttgcttggta tggcccttag gccctgtaga tcagcgtcct
     2821 acattaataa aggtagaact atatgtcact ggttttatag gctacatcag ctcatataga
     2881 tatgtcaacc aactcatcat ttctcttaag gacatgtggg ctattcacag tggttgtctc
     2941 tcctaaataa ataggccata ccaagcatgt ttgtgttcag atgtgtactt ttctggtatg
     3001 attttgaagg tagatttcca cggatgtaat tgtagagttg gtgtacaaac cgtgtggatt
     3061 tctacattgc tttccagaaa tgttaagtcc actcacagaa ctagcagtgt tgtccactgt
     3121 cctttgtgtt taaacatgtg gctaattgag aaacagtatg aacctcatcc atttatccat
     3181 tgtcagtagg tagctcagga tagccccaaa ttcatgatcc tcctgcctca gcctcccaaa
     3241 tgctggagtt agaagaatcc accgtgaggc tggcttatag ttttagaaat aaagtaattc
     3301 caagtgaagt acttctacac acattgtaaa ttctacaaga tgctttctac atagctcaaa
     3361 atgcaaaaat atttccaggc tcaggttatg cagagaaaac tgtacaattt ttgtcatttt
     3421 tttcatgtga tatttacagt gctattggaa cctagaattc acgcagtgaa aaatcactta
     3481 tgttttacat tgagagtata atcatttaat atagctgtat ttaactttca ggcaatattt
     3541 ttttcagtaa atcaatagtt aatacaatct caatcaggaa ggagccagca agctccttcc
     3601 ttccttcctt catttcctca tctgtcagtg atagaagtga gtaataaaat gtcttatgag
     3661 gtgatcccac atgggtagag ccattctgtg tctgtgtatc tgtgtgtgcg tgtgcatgca
     3721 tgtgtgtgcg tgtgtgtgtg tgtgtgtgtg tgtgtgcgtg tgtgtgtgtg tgtgtgtgtg
     3781 tgtttgagcc tgtctgactc acactcccca tgggatctgg agaattaagc tagagcttaa
     3841 ggaagtggct ctgtgcagaa gtcccccagt gtctggattg caagcagaaa gaagtactca
     3901 ttccttgtct ccactctttg ttcccattgg actccgtaca acctgctgtt gagccaagag
     3961 gagggaagaa gaagccctgg gaagtttttg aagaaagtta gagctctctg gctgccattg
     4021 ccagatatga tgtggccata tcgtcagaga gcagaacaca gaatggttcc ttgcccttcc
     4081 ttagctgcta ggccaaacct atgctgaaat ttaactgttt gagaaatggg cctcatgcac
     4141 aggaactgtt atccaactct cttctcatcc ctcatggaca cgtttgcttg taattacata
     4201 tcaatgggcc tgctgctgtg tggtagaatg ctctagtgga cataagatcc tgggtttaat
     4261 tcctatctca acaaaaagaa gagaaacaag atcagcagag tggaggggaa cagaatgaca
     4321 gactgactgc tgtgaataga ggtaactatt aggtcactga ctagttcatt cagggtagac
     4381 cagaaacccc ctgggtatta cagactgatg tccctgctgg gcatccagaa gcaatctttc
     4441 tgtggtcccc acctttgtgg ccagacattc tagtgataaa gctcagaaga cagatagtga
     4501 caataatact tttagggatg agaaggaacc aaacctgggg cttcatatgt cccaggtaag
     4561 aactccacca ttgagctgca cccctgtcct cataagtact caactgttag accgaagcag
     4621 actgcagtta tgtgttccac tgagcagcag gtttcatggg cctgacccgg gtgactccta
     4681 ctcagcagca gacaatgtga gcagtctaca gaggccacat gctgtgcact ctaggaaaac
     4741 cgctatgagt gtgagctgtt cca
//
