LOCUS       NM_001108462            3564 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus methylenetetrahydrofolate dehydrogenase (NADP+
            dependent) 1-like (Mthfd1l), mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_001108462 XM_001065359 XM_341750
VERSION     NM_001108462.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Momb,J., Lewandowski,J.P., Bryant,J.D., Fitch,R., Surman,D.R.,
            Vokes,S.A. and Appling,D.R.
  TITLE     Deletion of Mthfd1l causes embryonic lethality and neural tube and
            craniofacial defects in mice
  JOURNAL   Proc Natl Acad Sci U S A 110 (2), 549-554 (2013)
   PUBMED   23267094
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Watkins,D. and Rosenblatt,D.S.
  TITLE     Update and new concepts in vitamin responsive disorders of folate
            transport and metabolism
  JOURNAL   J Inherit Metab Dis 35 (4), 665-670 (2012)
   PUBMED   22108709
  REMARK    Review article
REFERENCE   3  (bases 1 to 3564)
  AUTHORS   Pike,S.T., Rajendra,R., Artzt,K. and Appling,D.R.
  TITLE     Mitochondrial C1-tetrahydrofolate synthase (MTHFD1L) supports the
            flow of mitochondrial one-carbon units into the methyl cycle in
            embryos
  JOURNAL   J Biol Chem 285 (7), 4612-4620 (2010)
   PUBMED   19948730
REFERENCE   4  (bases 1 to 3564)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   5  (bases 1 to 3564)
  AUTHORS   Parle-McDermott,A., Pangilinan,F., O'Brien,K.K., Mills,J.L.,
            Magee,A.M., Troendle,J., Sutton,M., Scott,J.M., Kirke,P.N.,
            Molloy,A.M. and Brody,L.C.
  TITLE     A common variant in MTHFD1L is associated with neural tube defects
            and mRNA splicing efficiency
  JOURNAL   Hum Mutat 30 (12), 1650-1656 (2009)
   PUBMED   19777576
REFERENCE   6  (bases 1 to 3564)
  AUTHORS   Walkup,A.S. and Appling,D.R.
  TITLE     Enzymatic characterization of human mitochondrial
            C1-tetrahydrofolate synthase
  JOURNAL   Arch Biochem Biophys 442 (2), 196-205 (2005)
   PUBMED   16171773
REFERENCE   7  (bases 1 to 3564)
  AUTHORS   Prasannan,P., Pike,S., Peng,K., Shane,B. and Appling,D.R.
  TITLE     Human mitochondrial C1-tetrahydrofolate synthase: gene structure,
            tissue distribution of the mRNA, and immunolocalization in Chinese
            hamster ovary calls
  JOURNAL   J Biol Chem 278 (44), 43178-43187 (2003)
   PUBMED   12937168
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC166462.1.
            
            On Dec 2, 2010 this sequence version replaced NM_001108462.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC166462.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1p11-q11"
     gene            1..3564
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /note="methylenetetrahydrofolate dehydrogenase (NADP+
                     dependent) 1-like"
                     /db_xref="GeneID:361472"
                     /db_xref="RGD:1307311"
     exon            1..329
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     CDS             106..3039
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /EC_number="6.3.4.3"
                     /note="formyltetrahydrofolate synthetase domain containing
                     1"
                     /codon_start=1
                     /product="monofunctional C1-tetrahydrofolate synthase,
                     mitochondrial"
                     /protein_id="NP_001101932.2"
                     /db_xref="GeneID:361472"
                     /db_xref="RGD:1307311"
                     /translation="MSARLPLLLRQLGHQQLPPGPACRLRELCRSGSRSSSSGGGDPE
                     GLRGRRLQDGQAFISHSPGSPEDPGMDSIVRDVIHNSKEVLNLLQEKNPAFKPVLAVI
                     QAGDDHLMQNVNQNLAKEAGLDITHICLPPDSGEDEIIDEILKINEDPRVHGLALQIS
                     EDSLCNKVLNALKPEKDVDGVTDVNLGKLVRGDAPECFVSPLARAAVELIEKSGITLD
                     GKKVLVIGAHGPLEAALQCLFQRKGSMTMSSPWITPQLQDKLREADIVVLGSPKPDEI
                     PAAWIPSGATMLNCFHDFLSGKLRGGSPGVPMNRLIADESVSLLAAALRVQNMVNSGR
                     RWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGK
                     SKAKVRLSLLERLKDQTDGKYVLVAGITPTPLGEGKSTVTIGLVQALTAHLNVNSFAC
                     LRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAAAIDTRIFH
                     ESTQTDKALYNRLVPLVNGVRKFSEIQLSRLKKLGIHKTDPTALTEEEVRKFTRLNID
                     PSTITWQRVLDTNDRFLRKITIGQGSTEKGYSRQAQFDIAVASEIMAVLALTDSLADM
                     KERLGRMVVASDKDGQPVTAEDLGVTGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFA
                     NIAHGNSSVLADKIALKLVGEEGFVVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLV
                     ATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQIAQLFGVPVVVAL
                     NVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSAGGKGSVDLARAVREAANKRSRFRF
                     LYDVQLPIVAKIRVIAQTVYGAKDIELSPEAQAKIDRYTQQGFGNLPICMAKTHLSLS
                     HEPDKKGVPRDFILPISDVRASIGAGFIYPLVGMMSTMPGLPTRPCFYDIDLDTETEQ
                     VKGLF"
     exon            330..414
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            415..465
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            466..519
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            520..644
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            645..745
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            746..882
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            883..994
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1086
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1184
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..1358
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..1495
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1542
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1650
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1725
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1828
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1829..1905
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1906..2046
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2047..2115
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2227
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2228..2367
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2368..2409
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2410..2510
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2688
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..2796
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2797..2949
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..3069
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            3070..3528
                     /gene="Mthfd1l"
                     /gene_synonym="Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgcttggttc tctctctcta ctgccttcct cgccaaccag gggaggaact tgcagccgca
       61 tctcggtgcc tccggaggac cagtcacacc gcgctctgca gcggcatgag tgcccgcctt
      121 cctctcctgc tacgccaact cggccaccag cagctgcccc cgggcccggc gtgtcgcctc
      181 cgtgagctgt gtcgatccgg cagcagaagc agcagcagcg gaggtggtga ccccgagggg
      241 ttgcgcgggc ggcggctgca ggatggccaa gccttcatca gccatagccc aggcagtccg
      301 gaagaccccg ggatggactc aatcgttaga gacgtcattc acaattccaa agaagtgctg
      361 aacttgttgc aagaaaaaaa ccctgccttt aagccagttc ttgctgtgat tcaggcagga
      421 gatgaccacc tgatgcagaa tgtgaaccag aatttggcca aggaggctgg cctggacatc
      481 actcacatct gcctacctcc agacagtgga gaggatgaga tcatagatga aatcctgaag
      541 attaatgaag accctagagt gcatggcctt gcccttcaga tctctgaaga ttcattgtgc
      601 aacaaagttc tcaatgcttt gaaaccggaa aaagatgtgg acggggtgac cgatgtcaat
      661 ctagggaagc tggtgcgagg tgacgctcct gaatgttttg tttcgccctt ggccagagct
      721 gcagtagaac tcattgagaa atcagggatc accttggatg gaaagaaggt cttggtcata
      781 ggggctcacg ggcctctgga agctgccctg caatgtcttt tccagagaaa aggatccatg
      841 acaatgagct ccccgtggat aacacctcag cttcaagaca agcttcgaga ggcagacatt
      901 gtggtcttgg gctcacctaa gcccgacgaa attcctgctg cttggatccc gtcaggagcc
      961 accatgctca attgcttcca tgacttcttg tcagggaagc tgagaggagg ttctcctggg
     1021 gtccccatga acagactcat tgccgacgag agcgtgagtc tccttgctgc agctctgcgg
     1081 gttcagaaca tggtgaacag tggcaggaga tggctccgag agcagcagca tagaagatgg
     1141 cgactccact gcttgaaact gcagcctctc tcccctgtgc ccagtgatat tgagatctca
     1201 cgaggacaga ctccaaaagc ggtggatgtc cttgccaagg agataggatt gcttgcagat
     1261 gaaattgaaa tctacggcaa gagcaaggcc aaagtccgct tgtccctgct ggagaggtta
     1321 aaggatcaaa cagatggaaa atacgtcttg gttgctggga tcacacccac cccacttgga
     1381 gaagggaaga gcacagtcac catcggacta gtgcaggcac taactgctca cctgaacgtc
     1441 aactccttcg cctgtctgag acagccttcc cagggaccca cttttggagt gaaaggagga
     1501 gctgcaggtg gtggatatgc tcaggtcatt cccatggagg agttcaacct tcacctgact
     1561 ggggacatcc acgccatcac tgccgctaat aacttactgg ctgctgccat cgacacgaga
     1621 attttccatg agagcactca gacagacaag gctctataca atcgactggt tcccttagtg
     1681 aatggtgtca gaaaattttc agaaattcaa ctttctcggc tgaagaaact ggggatacat
     1741 aagactgatc ccaccgctct gacagaagag gaagtgagga agttcacccg cctcaacatt
     1801 gacccctcta ccatcacatg gcagagagta ttggacacga atgaccgatt tctgagaaaa
     1861 ataactattg ggcagggaag cacggagaaa ggatattccc ggcaggcaca gtttgacatt
     1921 gcagtggcca gcgagatcat ggccgtgctg gccttgactg atagcctcgc agacatgaaa
     1981 gagcggctag gaagaatggt ggtggccagt gacaaagatg ggcagcctgt gacagctgag
     2041 gacttggggg tgacaggtgc tctgacagtt ttaatgaaag acgccatcaa gccaaatcta
     2101 atgcagaccc tggaaggaac acctgtgttt gtgcacgctg gtccctttgc taatatcgct
     2161 catggcaact cgtcggtgtt agctgataaa atcgcactaa aactggtcgg cgaagaaggg
     2221 tttgtagtga ctgaagctgg ctttggggct gatattggga tggagaaatt cttcaacatc
     2281 aaatgccgag cttctggcct ggtgcccaat gttgttgtcc tggtggccac cgttcgagct
     2341 ctgaagatgc atggaggtgg gccaagtgta accgctgggg ttcctctcaa gaaagaatat
     2401 acagaagaga acattcagct ggtagctgat ggctgctgca acctacagaa acaaattcag
     2461 atcgctcagc tcttcggggt gcccgtggtg gtggcactga atgtcttcaa gacggacacc
     2521 cgtgcagaga ttgacctggt gtgtgagctc gctaaacggg ccggtgcctt tgatgcggtc
     2581 ccctgctatc actggtcagc tggaggaaaa gggtccgtgg acttggctcg ggctgtgaga
     2641 gaagctgcga ataagagaag ccgcttccgg ttcctgtacg atgttcagct tcccatcgtg
     2701 gcaaagataa gagtaattgc ccagaccgtc tatggagcca aggatattga gctgtctcct
     2761 gaggcacagg ccaaaatcga ccgctatacg caacagggtt ttggaaattt gcccatctgc
     2821 atggcaaaga cccacctttc tctgtctcat gaacctgaca aaaagggggt gccgagggac
     2881 ttcattctgc ccatcagtga tgtccgagcc agcatcgggg ctgggttcat ttaccctttg
     2941 gtgggaatga tgagcacaat gccaggtctg cccacccgac cctgctttta cgatatagac
     3001 cttgacacag aaacagagca agttaaaggc ttgttctaat ggaatcgaac tctcacagga
     3061 ctgggccaga gtcttcaaac aaaaaggtct ctccttgcct ttttgctgca gttggagaag
     3121 aaaatgaatt ttgaaatctg cttgttatgc aatgctggag aaatcctgag acaggccaaa
     3181 gactgctttc ctcatgcaag atgaattctg cttccagcca agcggtgttc aacagaggga
     3241 cctgcaccac atctggaagg agcaaagtta ctctcggttc cgatagcgtt tacaccattt
     3301 ctgctgcttt tactttagaa agtttatttt atggaggcta ggggattgca ggagtgtgaa
     3361 ctaaaaacta atgtttcctt ctgtggggtt tcctctttca gctttttagc tctcccttcc
     3421 ctcacctgtg ttggggatgg gaggccaggc ctcggattgc actgtccagc actgtgctgc
     3481 caagccagtt tttgtctttg aactggaaat aaaagtattt ctagaaaaaa aaaaaaaaaa
     3541 aaaaaaaaaa aaaaaaaaaa aaaa
//
