LOCUS       NM_053307               1348 bp    mRNA    linear   ROD 30-JUL-2024
DEFINITION  Rattus norvegicus methionine sulfoxide reductase A (Msra), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_053307 XM_573806
VERSION     NM_053307.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Liu,J.S. and Cui,Z.J.
  TITLE     Pancreatic Stellate Cells Serve as a Brake Mechanism on Pancreatic
            Acinar Cell Calcium Signaling Modulated by Methionine Sulfoxide
            Reductase Expression
  JOURNAL   Cells 8 (2), 109 (2019)
   PUBMED   30717164
  REMARK    GeneRIF: Msr play important roles in pancreatic stellate cell
            function.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Fan,H., Wu,P.F., Zhang,L., Hu,Z.L., Wang,W., Guan,X.L., Luo,H.,
            Ni,M., Yang,J.W., Li,M.X., Chen,J.G. and Wang,F.
  TITLE     Methionine sulfoxide reductase A negatively controls
            microglia-mediated neuroinflammation via inhibiting
            ROS/MAPKs/NF-kappaB signaling pathways through a catalytic
            antioxidant function
  JOURNAL   Antioxid Redox Signal 22 (10), 832-847 (2015)
   PUBMED   25602783
  REMARK    GeneRIF: The study provides the first direct evidence for the
            biological significance of MsrA in microglia-mediated
            neuroinflammation.
REFERENCE   3  (bases 1 to 1348)
  AUTHORS   Wu,P.F., Zhang,Z., Guan,X.L., Li,Y.L., Zeng,J.H., Zhang,J.J.,
            Long,L.H., Hu,Z.L., Wang,F. and Chen,J.G.
  TITLE     A specific and rapid colorimetric method to monitor the activity of
            methionine sulfoxide reductase A
  JOURNAL   Enzyme Microb Technol 53 (6-7), 391-397 (2013)
   PUBMED   24315642
  REMARK    GeneRIF: Specific colorimetric microplate assay for testing MsrA
            activity quickly, based on the fact that MsrA can catalyze the
            reduction of methyl sulfoxides and simultaneously oxidize
            dithiothreitol, whose color can be produced by reacting with
            Ellman's reagent.
REFERENCE   4  (bases 1 to 1348)
  AUTHORS   Dun,Y., Vargas,J., Brot,N. and Finnemann,S.C.
  TITLE     Independent roles of methionine sulfoxide reductase A in
            mitochondrial ATP synthesis and as antioxidant in retinal pigment
            epithelial cells
  JOURNAL   Free Radic Biol Med 65, 1340-1351 (2013)
   PUBMED   24120970
  REMARK    GeneRIF: MsrA probably enhances ATP synthesis by increasing complex
            IV activity.
REFERENCE   5  (bases 1 to 1348)
  AUTHORS   Kim,J.Y., Choi,S.H., Lee,E., Kang,Y.J. and Kim,H.Y.
  TITLE     Methionine sulfoxide reductase A attenuates heme oxygenase-1
            induction through inhibition of Nrf2 activation
  JOURNAL   Arch Biochem Biophys 528 (2), 134-140 (2012)
   PUBMED   23036869
  REMARK    GeneRIF: MsrA overexpression in MsrA-depleted cells led to the
            reduction of increased HO-1 expression, and suppressed nuclear
            accumulation of Nrf2.
REFERENCE   6  (bases 1 to 1348)
  AUTHORS   Prentice,H.M., Moench,I.A., Rickaway,Z.T., Dougherty,C.J.,
            Webster,K.A. and Weissbach,H.
  TITLE     MsrA protects cardiac myocytes against hypoxia/reoxygenation
            induced cell death
  JOURNAL   Biochem Biophys Res Commun 366 (3), 775-778 (2008)
   PUBMED   18083115
  REMARK    GeneRIF: These observations indicate that MsrA is protective
            against hypoxia/reoxygenation stress in cardiac myocytes and point
            to MsrA as an important therapeutic target for ischemic heart
            disease.
REFERENCE   7  (bases 1 to 1348)
  AUTHORS   Haenold,R., Wassef,R., Hansel,A., Heinemann,S.H. and Hoshi,T.
  TITLE     Identification of a new functional splice variant of the enzyme
            methionine sulphoxide reductase A (MSRA) expressed in rat vascular
            smooth muscle cells
  JOURNAL   Free Radic Res 41 (11), 1233-1245 (2007)
   PUBMED   17907003
  REMARK    GeneRIF: Multiple MSRA variants participate in the repair of
            oxidized proteins in vascular smooth muscle cells mitochondria.
REFERENCE   8  (bases 1 to 1348)
  AUTHORS   Yermolaieva,O., Xu,R., Schinstock,C., Brot,N., Weissbach,H.,
            Heinemann,S.H. and Hoshi,T.
  TITLE     Methionine sulfoxide reductase A protects neuronal cells against
            brief hypoxia/reoxygenation
  JOURNAL   Proc Natl Acad Sci U S A 101 (5), 1159-1164 (2004)
   PUBMED   14745014
  REMARK    GeneRIF: Methionine sulfoxide reductase A plays a protective role
            against hypoxia/reoxygenation-induced cell injury.
REFERENCE   9  (bases 1 to 1348)
  AUTHORS   Moskovitz,J., Bar-Noy,S., Williams,W.M., Requena,J., Berlett,B.S.
            and Stadtman,E.R.
  TITLE     Methionine sulfoxide reductase (MsrA) is a regulator of antioxidant
            defense and lifespan in mammals
  JOURNAL   Proc Natl Acad Sci U S A 98 (23), 12920-12925 (2001)
   PUBMED   11606777
REFERENCE   10 (bases 1 to 1348)
  AUTHORS   Petropoulos,I., Mary,J., Perichon,M. and Friguet,B.
  TITLE     Rat peptide methionine sulphoxide reductase: cloning of the cDNA,
            and down-regulation of gene expression and enzyme activity during
            aging
  JOURNAL   Biochem J 355 (Pt 3), 819-825 (2001)
   PUBMED   11311146
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC087009.1.
            
            On or before Apr 19, 2005 this sequence version replaced
            XM_573806.1, NM_053307.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC087009.1, CK480500.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760476 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     gene            1..1348
                     /gene="Msra"
                     /note="methionine sulfoxide reductase A"
                     /db_xref="GeneID:29447"
                     /db_xref="RGD:70979"
     exon            1..146
                     /gene="Msra"
                     /inference="alignment:Splign:2.1.0"
     CDS             11..712
                     /gene="Msra"
                     /EC_number="1.8.4.11"
                     /note="peptide methionine sulfoxide reductase; PMSR;
                     peptide Met(O) reductase; protein-methionine-S-oxide
                     reductase; peptide-methionine (S)-S-oxide reductase"
                     /codon_start=1
                     /product="mitochondrial peptide methionine sulfoxide
                     reductase"
                     /protein_id="NP_445759.1"
                     /db_xref="GeneID:29447"
                     /db_xref="RGD:70979"
                     /translation="MLSASRRTLQLLSSSIPVRMMGDSSSKVISAEEALPGRTESIPV
                     AAKHHVSGNRTVEPFPEGTQMAVFGMGCFWGAERKFWLLKGVYSTQVGFAGGYTRNPT
                     YKEVCSEKTGHAEVVRVVYRPEHVSFEELLKVFWENHDPTQGMRQGNDCGTQYRSAVY
                     PTSAVQMEAALKSKEEYQKVLSKHGFGPITTDIREGQVFYYAEDYHQQYLSKNPDGYC
                     GLGGTGVSCPTAIKK"
     exon            147..215
                     /gene="Msra"
                     /inference="alignment:Splign:2.1.0"
     exon            216..335
                     /gene="Msra"
                     /inference="alignment:Splign:2.1.0"
     exon            336..440
                     /gene="Msra"
                     /inference="alignment:Splign:2.1.0"
     exon            441..547
                     /gene="Msra"
                     /inference="alignment:Splign:2.1.0"
     exon            548..1300
                     /gene="Msra"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cacgctgtcc atgctctccg cctccagaag gactctccag ctcctctcca gctccatccc
       61 ggtacggatg atgggcgact catcttcgaa ggtcatcagc gcggaggaag ctttgccagg
      121 acgcacagag tcgatccccg tagcagccaa acaccatgtc agtggcaaca gaacggttga
      181 acctttccca gagggaacac agatggctgt atttggaatg ggctgcttct ggggagctga
      241 gcgcaagttc tggctcttga aaggagtgta ttcaacccaa gtgggtttcg caggaggcta
      301 cacacgcaat cccacctaca aagaggtctg ctcagaaaaa accggtcacg cagaagtcgt
      361 ccgggttgtg taccggccag agcacgtcag ctttgaggag ctgctcaagg tcttctggga
      421 gaatcacgac ccgacccaag ggatgcgcca aggcaatgac tgtggcacgc agtatcgatc
      481 ggcggtctat cccacctccg ccgtgcagat ggaagctgcc ctgaaatcca aggaggagta
      541 ccagaaggtt ctttcaaagc atggctttgg ccccatcacc accgacatcc gagagggaca
      601 agtgttctac tacgccgaag attaccacca gcaatacctg agcaagaacc cagacggcta
      661 ctgtggcctt gggggcactg gggtttcctg tcccacggcc attaaaaagt aatttctctc
      721 cacgaagctg gccctggagg ttccatcaac accatgattg ctggatggag cagcactgct
      781 gtgattccca tctgtcactt tatgaagagc acacaccaca cgggcattta catgaatctg
      841 gttgttatcc gggtctgatt tacagaaagt aggatgacga ggtgatgcgt tgtaatttgt
      901 cttctgatca gtcgggatgg ggatagaaca tgttttagtg gatcatttgg ggtctaaatg
      961 aagataacga atatcctgtg tccatctgct ggagccggag ggatgctaag ggttctgaaa
     1021 cagggagctg gctgggagag cacggagcac tgggactcta gggctgacat acctcgccca
     1081 cctccctgcc ctcaggtgcc taattcatta gtgtctagga gctccacgaa tcattgccga
     1141 aacactttgc tctgctgcct ctagacgcat attaggggtt tctctccccc ctccgtcctc
     1201 tctgtgcaga gaaaagacat gagtgacctt aatgaattct aacattctgc ttacagctat
     1261 gataaagctt gttctaataa aaatctacgg tttggtgata aaaaaaaaaa aaaaaaaaaa
     1321 aaaaaaaaaa aaaaaaaaaa aaaaaaaa
//
