LOCUS       NM_017027               2018 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Rattus norvegicus myelin protein zero (Mpz), transcript variant 1,
            mRNA.
ACCESSION   NM_017027
VERSION     NM_017027.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Chang,E.H., Mo,W.M., Doo,H.M., Lee,J.S., Park,H.T., Choi,B.O. and
            Hong,Y.B.
  TITLE     Aminosalicylic acid reduces ER stress and Schwann cell death
            induced by MPZ mutations
  JOURNAL   Int J Mol Med 44 (1), 125-134 (2019)
   PUBMED   31059078
  REMARK    GeneRIF: the findings of this study indicate that treatment with
            4ASA reduced the ER stress and Schwann cell death caused by 2
            different MPZ mutants and suggest that ASA may be a potential
            therapeutic agent for CharcotMarieTooth disease .
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Yamaguchi,Y. and Baba,H.
  TITLE     Phylogenetically Conserved Sequences Around Myelin P0 Stop Codon
            are Essential for Translational Readthrough to Produce L-MPZ
  JOURNAL   Neurochem Res 43 (1), 227-237 (2018)
   PUBMED   29081003
  REMARK    GeneRIF: that phylogenetic emergence of L-MPZ in amphibians may be
            related to particular distribution and/or function in the PNS
            myelin
REFERENCE   3  (bases 1 to 2018)
  AUTHORS   Zhang,X., Xin,N., Tong,L. and Tong,X.J.
  TITLE     Electrical stimulation enhances peripheral nerve regeneration after
            crush injury in rats
  JOURNAL   Mol Med Rep 7 (5), 1523-1527 (2013)
   PUBMED   23545781
REFERENCE   4  (bases 1 to 2018)
  AUTHORS   Yamaguchi,Y., Hayashi,A., Campagnoni,C.W., Kimura,A., Inuzuka,T.
            and Baba,H.
  TITLE     L-MPZ, a novel isoform of myelin P0, is produced by stop codon
            readthrough
  JOURNAL   J Biol Chem 287 (21), 17765-17776 (2012)
   PUBMED   22457349
  REMARK    GeneRIF: L-MPZ, a novel isoform of myelin P0, is produced by stop
            codon readthrough.
REFERENCE   5  (bases 1 to 2018)
  AUTHORS   Beier,H. and Grimm,M.
  TITLE     Misreading of termination codons in eukaryotes by natural nonsense
            suppressor tRNAs
  JOURNAL   Nucleic Acids Res 29 (23), 4767-4782 (2001)
   PUBMED   11726686
  REMARK    Review article
REFERENCE   6  (bases 1 to 2018)
  AUTHORS   Shapiro,L., Doyle,J.P., Hensley,P., Colman,D.R. and
            Hendrickson,W.A.
  TITLE     Crystal structure of the extracellular domain from P0, the major
            structural protein of peripheral nerve myelin
  JOURNAL   Neuron 17 (3), 435-449 (1996)
   PUBMED   8816707
REFERENCE   7  (bases 1 to 2018)
  AUTHORS   Cheng,L. and Mudge,A.W.
  TITLE     Cultured Schwann cells constitutively express the myelin protein P0
  JOURNAL   Neuron 16 (2), 309-319 (1996)
   PUBMED   8789946
REFERENCE   8  (bases 1 to 2018)
  AUTHORS   D'Urso,D., Brophy,P.J., Staugaitis,S.M., Gillespie,C.S., Frey,A.B.,
            Stempak,J.G. and Colman,D.R.
  TITLE     Protein zero of peripheral nerve myelin: biosynthesis, membrane
            insertion, and evidence for homotypic interaction
  JOURNAL   Neuron 4 (3), 449-460 (1990)
   PUBMED   1690568
REFERENCE   9  (bases 1 to 2018)
  AUTHORS   Lemke,G., Lamar,E. and Patterson,J.
  TITLE     Isolation and analysis of the gene encoding peripheral myelin
            protein zero
  JOURNAL   Neuron 1 (1), 73-83 (1988)
   PUBMED   2483091
REFERENCE   10 (bases 1 to 2018)
  AUTHORS   Lemke,G. and Axel,R.
  TITLE     Isolation and sequence of a cDNA encoding the major structural
            protein of peripheral myelin
  JOURNAL   Cell 40 (3), 501-508 (1985)
   PUBMED   2578885
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB608173.1 and BC078859.1.
            
            On Oct 5, 2015 this sequence version replaced NM_017027.1.
            
            Summary: This gene is specifically expressed in Schwann cells of
            the peripheral nervous system and encodes a type I transmembrane
            glycoprotein that is a major structural protein of the peripheral
            myelin sheath. The encoded protein contains a large hydrophobic
            extracellular domain and a smaller basic intracellular domain,
            which are essential for the formation and stabilization of the
            multilamellar structure of the compact myelin. Mutations in the
            orthologous gene in human are associated with myelinating
            neuropathies. A recent study showed that two isoforms are produced
            from the same mRNA by use of alternative in-frame translation
            termination codons via a stop codon readthrough mechanism.
            [provided by RefSeq, Oct 2015].
            
            Transcript Variant: This transcript (1) encodes two isoforms, which
            result from the use of alternative in-frame translation termination
            codons. The shorter isoform (MPZ, also known as P0) results from
            translation termination at the upstream UAG stop codon, while the
            longer isoform (L-MPZ) results from UAG stop codon readthrough to
            the downstream UGA termination codon. This RefSeq represents the
            shorter isoform (MPZ).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC078859.1, K03242.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            RefSeq Select criteria           :: based on single protein-coding
                                                transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-139               CB608173.1         1-139
            140-2018            BC078859.1         1-1879
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q24"
     gene            1..2018
                     /gene="Mpz"
                     /gene_synonym="MPP; P0"
                     /note="myelin protein zero"
                     /db_xref="GeneID:24564"
                     /db_xref="RGD:3109"
     exon            1..237
                     /gene="Mpz"
                     /gene_synonym="MPP; P0"
                     /inference="alignment:Splign:2.1.0"
     CDS             171..917
                     /gene="Mpz"
                     /gene_synonym="MPP; P0"
                     /note="isoform MPZ precursor is encoded by transcript
                     variant 1; myelin protein zero (Charcot-Marie-Tooth
                     neuropathy 1B); myelin protein P0; myelin peripheral
                     protein"
                     /codon_start=1
                     /product="myelin protein P0 isoform MPZ precursor"
                     /protein_id="NP_058723.2"
                     /db_xref="GeneID:24564"
                     /db_xref="RGD:3109"
                     /translation="MAPGAPSSSPSPILAALLFSSLVLSPTLAIVVYTDREVYGAVGS
                     QVTLHCSFWSSEWVSDDISFTWRYQPEGGRDAISIFHYAKGQPYIDEVGTFKERIQWV
                     GDPSWKDGSIVIHNLDYSDNGTFTCDVKNPPDIVGKTSQVTLYVFEKVPTRYGVVLGA
                     VIGGILGVVLLLLLLFYLIRYCWLRRQAALQRRLSAMEKGKFHKSSKDSSKRGRQTPV
                     LYAMLDHSRSTKAASEKKSKGLGESRKDKK"
     exon            238..404
                     /gene="Mpz"
                     /gene_synonym="MPP; P0"
                     /inference="alignment:Splign:2.1.0"
     exon            405..618
                     /gene="Mpz"
                     /gene_synonym="MPP; P0"
                     /inference="alignment:Splign:2.1.0"
     exon            619..754
                     /gene="Mpz"
                     /gene_synonym="MPP; P0"
                     /inference="alignment:Splign:2.1.0"
     exon            755..815
                     /gene="Mpz"
                     /gene_synonym="MPP; P0"
                     /inference="alignment:Splign:2.1.0"
     exon            816..1991
                     /gene="Mpz"
                     /gene_synonym="MPP; P0"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1969..1974
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mpz"
                     /gene_synonym="MPP; P0"
     polyA_site      1991
                     /gene="Mpz"
                     /gene_synonym="MPP; P0"
ORIGIN      
        1 ccacctctca attgcacatg ccaggctgca attggtcact ggctcaggac agccccctca
       61 tgctggggat ccaggggatt ttaagcaggt tccagaaaac accactcagt tccttgtccc
      121 ccgctctctc caccccacag acgctctggg cccttgcccc taccccagct atggctcctg
      181 gggctccctc atccagcccc agccctatcc tggctgccct gctcttctct tctttggtgc
      241 tgtccccaac cctggccatt gtggtttaca cggacaggga agtctatggt gctgtgggct
      301 cccaggtgac cctgcactgc tccttctggt ccagtgaatg ggtctcagat gacatctctt
      361 ttacctggcg ctaccagcct gaaggaggcc gagatgccat ttcaatcttc cactatgcca
      421 agggtcaacc ttacatcgat gaggtgggga ccttcaagga gcgcatccag tgggtagggg
      481 accctagctg gaaggatggc tccattgtca tacacaacct agactacagt gacaacggca
      541 ctttcacatg tgatgtcaaa aacccaccgg acatagtggg caagacgtct caggtcacgc
      601 tctatgtctt tgaaaaagtg cccactaggt atggggtggt gttgggagcc gtgatcggtg
      661 gcatcctcgg ggtggtgctg ttgctgctgt tgctcttcta cctgatccgg tactgctggc
      721 tgcgcaggca ggctgccctg cagaggaggc tcagtgccat ggagaagggg aaatttcaca
      781 agtcttctaa ggactcctcg aagcgcgggc ggcagacgcc agtgctgtat gccatgctgg
      841 accacagccg aagcaccaaa gctgccagtg agaagaaatc taaagggctg ggggagtctc
      901 gcaaggataa gaaatagcgg ttagcgggcc gggcgggggg tcgggggtct gcgatggagt
      961 cttccaaagg ctctcaggtg gtggtcatcg agatggagct tcgcaaagat gagcagagct
     1021 cggagctccg gcctgcagtc aaatccccca gtagaaccag cctcaagaac gccctcaaga
     1081 acatgatggg cctggactcg gacaagtgac cgtcacctcc aggctctgtc agagcagggg
     1141 acctaggctc ctctttttcc caggtctaga tgctttcccc ttgctcctca gccctgccct
     1201 gcccttacct cccaatgaga tgtaaagttt cattccaatg ttccttccca agtcttgtat
     1261 ttctccttca ctttttcccc tcggctttcc gggagcctag ggactaactc tactcctcac
     1321 ctgccccaag ggctgtgtgt ttggtgcctc tccctcaggc accgagaaga aaggaacctt
     1381 gatagcctct gcccaactcc aggccactgg gccttcccat ctcctcctcc ggttcccctg
     1441 gctccttcct gctgtctcct ttcctacctt gtggtggccc agctccacac tctgtcctcg
     1501 ggctcaaatc cacatcatgc agatcagact ctccatcagg gtttatctag gttgctgagt
     1561 attttttatt tgttcatcca tcctttgttt acctgagccc acggccccat gaccgaggac
     1621 caatgacgtc atgtggcttt tgcagttccc cactcctatt aagtctttac tggagagcca
     1681 gccaggcaag cagagcataa ttgaggccct ctgactgacc actctggagc acagttctga
     1741 gactccgaga tttggggaag gagagagaga aaaaatggat cttcacaggt caggggtgca
     1801 gtagggagct tcaagaaacg tcttcgaaat aagtaataag gagaacaggg aaagccaatg
     1861 agaataggaa ggaaaggaag tggttgcact cccaggcaca ggggactgtt aggacttttc
     1921 tacattctgt atatttctcc taccatctcc aaatgtcctt aaatgtttaa taaacactga
     1981 catttccagg aaaaaaaaaa aaaaaaaaaa aaaaaaaa
//
