LOCUS       NM_138843               1248 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus mercaptopyruvate sulfurtransferase (Mpst), mRNA.
ACCESSION   NM_138843 XM_346800
VERSION     NM_138843.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Zhang,F., Chen,S., Wen,J.Y. and Chen,Z.W.
  TITLE     3-Mercaptopyruvate sulfurtransferase/hydrogen sulfide protects
            cerebral endothelial cells against oxygen-glucose
            deprivation/reoxygenation-induced injury via mitoprotection and
            inhibition of the RhoA/ROCK pathway
  JOURNAL   Am J Physiol Cell Physiol 319 (4), C720-C733 (2020)
   PUBMED   32813542
  REMARK    GeneRIF: 3-Mercaptopyruvate sulfurtransferase/hydrogen sulfide
            protects cerebral endothelial cells against oxygen-glucose
            deprivation/reoxygenation-induced injury via mitoprotection and
            inhibition of the RhoA/ROCK pathway.
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Bronowicka-Adamska,P., Hutsch,T., Gawrys-Kopczynska,M.,
            Maksymiuk,K. and Wrobel,M.
  TITLE     Hydrogen sulfide formation in experimental model of acute
            pancreatitis
  JOURNAL   Acta Biochim Pol 66 (4), 611-618 (2019)
   PUBMED   31893496
  REMARK    GeneRIF: The study revealed a significant role of MPST in hydrogen
            sulfide metabolism in the pancreas in experimental model of acute
            pancreatitis.
REFERENCE   3  (bases 1 to 1248)
  AUTHORS   Nagahara,N., Koike,S., Nirasawa,T., Kimura,H. and Ogasawara,Y.
  TITLE     Alternative pathway of H2S and polysulfides production from
            sulfurated catalytic-cysteine of reaction intermediates of
            3-mercaptopyruvate sulfurtransferase
  JOURNAL   Biochem Biophys Res Commun 496 (2), 648-653 (2018)
   PUBMED   29331374
  REMARK    GeneRIF: Presented is alternative pathway of production of hydrogen
            sulfide and/or polysulfides from (poly)sulfurated catalytic-site
            cysteine of reaction intermediates of MST via thioredoxin
            reduction.
REFERENCE   4  (bases 1 to 1248)
  AUTHORS   Hanaoka,K., Sasakura,K., Suwanai,Y., Toma-Fukai,S., Shimamoto,K.,
            Takano,Y., Shibuya,N., Terai,T., Komatsu,T., Ueno,T., Ogasawara,Y.,
            Tsuchiya,Y., Watanabe,Y., Kimura,H., Wang,C., Uchiyama,M.,
            Kojima,H., Okabe,T., Urano,Y., Shimizu,T. and Nagano,T.
  TITLE     Discovery and Mechanistic Characterization of Selective Inhibitors
            of H2S-producing Enzyme: 3-Mercaptopyruvate Sulfurtransferase
            (3MST) Targeting Active-site Cysteine Persulfide
  JOURNAL   Sci Rep 7, 40227 (2017)
   PUBMED   28079151
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1248)
  AUTHORS   Kuo,M.M., Kim,D.H., Jandu,S., Bergman,Y., Tan,S., Wang,H.,
            Pandey,D.R., Abraham,T.P., Shoukas,A.A., Berkowitz,D.E. and
            Santhanam,L.
  TITLE     MPST but not CSE is the primary regulator of hydrogen sulfide
            production and function in the coronary artery
  JOURNAL   Am J Physiol Heart Circ Physiol 310 (1), H71-H79 (2016)
   PUBMED   26519030
  REMARK    GeneRIF: 3-Mercaptopyruvate sulphurtransferase and not
            cystathionine gamma-lyase is the primary regulator of coronary
            artery hydrogen sulfide production and function.
REFERENCE   6  (bases 1 to 1248)
  AUTHORS   Nagahara,N., Ito,T., Kitamura,H. and Nishino,T.
  TITLE     Tissue and subcellular distribution of mercaptopyruvate
            sulfurtransferase in the rat: confocal laser fluorescence and
            immunoelectron microscopic studies combined with biochemical
            analysis
  JOURNAL   Histochem Cell Biol 110 (3), 243-250 (1998)
   PUBMED   9749958
REFERENCE   7  (bases 1 to 1248)
  AUTHORS   Slavin,A.J., Johns,T.G., Orian,J.M. and Bernard,C.C.
  TITLE     Regulation of myelin oligodendrocyte glycoprotein in different
            species throughout development
  JOURNAL   Dev Neurosci 19 (1), 69-78 (1997)
   PUBMED   9078435
REFERENCE   8  (bases 1 to 1248)
  AUTHORS   Nagahara,N. and Nishino,T.
  TITLE     Role of amino acid residues in the active site of rat liver
            mercaptopyruvate sulfurtransferase. CDNA cloning, overexpression,
            and site-directed mutagenesis
  JOURNAL   J Biol Chem 271 (44), 27395-27401 (1996)
   PUBMED   8910318
REFERENCE   9  (bases 1 to 1248)
  AUTHORS   Nagahara,N., Okazaki,T. and Nishino,T.
  TITLE     Cytosolic mercaptopyruvate sulfurtransferase is evolutionarily
            related to mitochondrial rhodanese. Striking similarity in active
            site amino acid sequence and the increase in the mercaptopyruvate
            sulfurtransferase activity of rhodanese by site-directed
            mutagenesis
  JOURNAL   J Biol Chem 270 (27), 16230-16235 (1995)
   PUBMED   7608189
REFERENCE   10 (bases 1 to 1248)
  AUTHORS   Kuo,S.M., Lea,T.C. and Stipanuk,M.H.
  TITLE     Developmental pattern, tissue distribution, and subcellular
            distribution of cysteine: alpha-ketoglutarate aminotransferase and
            3-mercaptopyruvate sulfurtransferase activities in the rat
  JOURNAL   Biol Neonate 43 (1-2), 23-32 (1983)
   PUBMED   6573924
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Nov 24, 2020 this sequence version replaced NM_138843.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ227898.1, FQ232045.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                JAXUCZ010000007.1  111836193-111836219
            28-649              JAXUCZ010000007.1  111838859-111839480
            650-1248            JAXUCZ010000007.1  111843053-111843651
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q34"
     gene            1..1248
                     /gene="Mpst"
                     /gene_synonym="Mst"
                     /note="mercaptopyruvate sulfurtransferase"
                     /db_xref="GeneID:192172"
                     /db_xref="RGD:620065"
     exon            1..27
                     /gene="Mpst"
                     /gene_synonym="Mst"
                     /inference="alignment:Splign:2.1.0"
     exon            28..649
                     /gene="Mpst"
                     /gene_synonym="Mst"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..948
                     /gene="Mpst"
                     /gene_synonym="Mst"
                     /EC_number="2.8.1.2"
                     /codon_start=1
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="NP_620198.1"
                     /db_xref="GeneID:192172"
                     /db_xref="RGD:620065"
                     /translation="MAAPQLFRALVSAQWVAEALKSPRASQPLKLLDASWYLPKLGRD
                     ARREFEERHIPGAAFFDIDRCSDHTSPYDHMLPSATHFADYAGSLGVSAATHVVIYDG
                     SDQGLYSAPRVWWMFRAFGHHSVSLLDGGFRYWLSQNLPISSGKSPSEPAEFCAQLDP
                     SFIKTHEDILENLDARRFQVVDARAAGRFQGTQPEPRDGIEPGHIPGSVNIPFTEFLT
                     SEGLEKSPEEIQRLFQEKKVDLSKPLVATCGSGVTACHVVLGAFLCGKPDVPVYDGSW
                     VEWYMRAQPEHVISQGRGKTL"
     exon            650..1248
                     /gene="Mpst"
                     /gene_synonym="Mst"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aagtctcctc ccttcggctg gccgcagccc tgcaatccga gcgtccccta ggccatggct
       61 gccccacagc ttttccgagc tctggtatct gctcagtggg tggcggaggc tctgaaatcc
      121 cctcgcgcct ctcagccgct gaagttactg gatgcgtcct ggtacctacc caagctgggc
      181 cgcgatgcgc gacgcgagtt tgaggagcgc cacatcccgg gcgccgcctt ctttgacatc
      241 gatcgttgca gcgaccacac atcgccctac gaccacatgc tgcctagtgc cacgcatttc
      301 gccgactacg cgggcagctt gggcgtgagt gccgctacac acgtcgtgat ctacgacggc
      361 agcgaccagg gcctctattc cgctccgagg gtctggtgga tgttccgtgc cttcggccac
      421 cactctgtgt cgttgctgga cggcggcttc cgctattggc tgagccagaa cctgccaatt
      481 agctctggca aaagcccctc ggaacctgcg gagttctgcg cgcagctcga tccctccttc
      541 atcaagaccc acgaggatat cctggagaac ttggatgccc ggcgcttcca ggtagtggac
      601 gcccgcgcag ctggccgttt ccaaggcaca cagccagaac cccgagatgg catcgaacct
      661 ggacacatcc ctggctcagt aaacatccca ttcactgaat tcctgaccag tgaaggtctg
      721 gagaagagcc cagaggagat ccaacgcctg ttccaggaga agaaggtgga cctgtccaag
      781 cccttggtag ccacatgcgg ctccggtgtc acagcctgcc acgtggtcct gggggccttc
      841 ctctgtggca aacccgatgt gcctgtctac gatggctcct gggtggagtg gtacatgcgt
      901 gcccaaccgg agcacgtcat ctctcagggc cgggggaaga ccctgtgaag gacacagtgc
      961 agcttgggtg acaccgaacc atcctagtgc cagcctggtc actctgaccc tgctttgcca
     1021 agagtccttc ctcagacaac tcaggtggtg tttaaggtga tatcccagag accaggagtt
     1081 tcagtgactc ctgggcactc agtggaggta gccgagaggt ggcagacagt aagagggtac
     1141 ccaggggagg gagggaggcc agacagtaga acagcttaca tggtgctaag ctggggccca
     1201 cctagcttgt ttcactgttg ggaaataaaa catcaagcac aaaacctt
//
