LOCUS       NM_001305460            4202 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus macrophage expressed 1 (Mpeg1), transcript
            variant 1, mRNA.
ACCESSION   NM_001305460 XM_006231115
VERSION     NM_001305460.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Strbo,N., Pastar,I., Romero,L., Chen,V., Vujanac,M., Sawaya,A.P.,
            Jozic,I., Ferreira,A.D.F., Wong,L.L., Head,C., Stojadinovic,O.,
            Garcia,D., O'Neill,K., Drakulich,S., Taller,S., Kirsner,R.S. and
            Tomic-Canic,M.
  TITLE     Single cell analyses reveal specific distribution of anti-bacterial
            molecule Perforin-2 in human skin and its modulation by wounding
            and Staphylococcus aureus infection
  JOURNAL   Exp Dermatol 28 (3), 225-232 (2019)
   PUBMED   30609079
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   McCormack,R.M., de Armas,L.R., Shiratsuchi,M., Fiorentino,D.G.,
            Olsson,M.L., Lichtenheld,M.G., Morales,A., Lyapichev,K.,
            Gonzalez,L.E., Strbo,N., Sukumar,N., Stojadinovic,O., Plano,G.V.,
            Munson,G.P., Tomic-Canic,M., Kirsner,R.S., Russell,D.G. and
            Podack,E.R.
  TITLE     Perforin-2 is essential for intracellular defense of parenchymal
            cells and phagocytes against pathogenic bacteria
  JOURNAL   Elife 4, e06508 (2015)
   PUBMED   26402460
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4202)
  AUTHORS   Fields,K.A., McCormack,R., de Armas,L.R. and Podack,E.R.
  TITLE     Perforin-2 restricts growth of Chlamydia trachomatis in macrophages
  JOURNAL   Infect Immun 81 (8), 3045-3054 (2013)
   PUBMED   23753625
REFERENCE   4  (bases 1 to 4202)
  AUTHORS   McCormack,R., de Armas,L.R., Shiratsuchi,M., Ramos,J.E. and
            Podack,E.R.
  TITLE     Inhibition of intracellular bacterial replication in fibroblasts is
            dependent on the perforin-like protein (perforin-2) encoded by
            macrophage-expressed gene 1
  JOURNAL   J Innate Immun 5 (2), 185-194 (2013)
   PUBMED   23257510
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1 and AI511268.1.
            
            On Mar 15, 2015 this sequence version replaced XM_006231115.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript from the same
            strain was available for the full length of the gene. The extent of
            this transcript is supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643429.5123.1,
                                           SRR26643293.8074.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-119               JAXUCZ010000001.1  218876894-218877012
            120-3875            JAXUCZ010000001.1  218877295-218881050
            3876-4202           AI511268.1         1-327               c
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q43"
     gene            1..4202
                     /gene="Mpeg1"
                     /gene_synonym="Mpg-1; P-2"
                     /note="macrophage expressed 1"
                     /db_xref="GeneID:64552"
                     /db_xref="RGD:69275"
     exon            1..119
                     /gene="Mpeg1"
                     /gene_synonym="Mpg-1; P-2"
                     /inference="alignment:Splign:2.1.0"
     exon            120..4189
                     /gene="Mpeg1"
                     /gene_synonym="Mpg-1; P-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             149..2314
                     /gene="Mpeg1"
                     /gene_synonym="Mpg-1; P-2"
                     /note="macrophage-expressed gene 1 protein; perforin-2"
                     /codon_start=1
                     /product="macrophage-expressed gene 1 protein precursor"
                     /protein_id="NP_001292389.1"
                     /db_xref="GeneID:64552"
                     /db_xref="RGD:69275"
                     /translation="MAGNACTMNSFMAIALIWMMIACAEADKPLRDPGMTGFQTCKDT
                     LKLPVLEVLPGGGWDNLRNIDMGRVIDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLE
                     MNSEILDSWVNYQSTTSFSINTELSLFSKVNGKFSTEFQRMKTLQVKDQAVTTRVQVR
                     NRIYTVKNSPTSELSFGFTNALMDICDQLEKNQTKMATYLAELLVLNYGTHVITSVDA
                     GAALVQEDHIRSSFLLDNQNSENTVTASAGIAFLNIVNFKVETDHTSQTTLTKSYLSN
                     RTNSRVQSFGGIPFYPGITLETWQKGITNHLVAIDRAGLPLHFFIKPDKLPGLPGPLV
                     KKLSKTVETAVRHYYTFNTHPGCTNVDSPNFNFQANMEDDSCDAKVTNFTFGGLYQEC
                     TELSGDALCQNLEQKNLLTGDFSCPSGYTPVHLLSQTHEEGYSRLECKKKCTLKIFCK
                     TVCEDVFRVAKAQFRAYWCVATGQVPDNSGLLFGGLFTDKSINPMTNAQSCPAGYIPL
                     NLFESLKVCVSLDYELGYKFSVPFGGFFSCIMGNPLVNSDTAKDIGAPSLKKCPGGFS
                     QHLAVISDGCQVSYCVKAGIFTGGSLLPVRLPPYTKPPLMSQVATNTVIVTSSETARS
                     WIKDPQTNQWKLGEPLELHRAMTVIHGDGNGMSGGEAAGVTLGVIIALGIVITLAIYS
                     TRKYKKEKEYQEIEEQESLVGSFATDASAPNGEQDPCPA"
ORIGIN      
        1 agtaaaaaga aactaatatt gtgatttcac aagaaaggag taaggctgat tttgaagtca
       61 gttaaagaga gccaaaagcc agacagagcc ttcagacaga gccttctgac agagacaagt
      121 cttgctggtg aatgcctggg actggtatat ggctggaaac gcctgcacca tgaacagctt
      181 catggccatt gccctcatct ggatgatgat agcatgtgct gaagcagaca agcctctcag
      241 agacccgggt atgactggat ttcaaacatg caaggatacc ttgaagctac ctgtcttgga
      301 ggtcctgcca ggaggaggct gggataatct gagaaacatt gacatgggac gggtgataga
      361 cttgacctac accaactgta agaccacaga agatggacag tacattatcc ctgatgaagt
      421 ctatactatt cctcagaagg agagcaacct ggagatgaat tcagaaatcc tggattcctg
      481 ggtgaattac cagagtacca cctcattttc catcaacacg gaactctccc ttttctccaa
      541 agtcaatggc aagttttcta cagagttcca aaggatgaag acccttcaag taaaggacca
      601 agctgtaact actagagttc aggtaagaaa ccggatctac acagtcaaaa acagcccaac
      661 ttcagagctc agcttcgggt ttacgaacgc acttatggac atctgtgacc aattagagaa
      721 aaaccagacg aagatggcca cctacctggc agaactcttg gtcctcaact atggcacaca
      781 tgttatcacc agtgtggatg ctggggctgc actggttcag gaggatcaca tcaggtcctc
      841 cttcctcctg gacaaccaga acagtgagaa tactgtgact gcctctgctg ggattgcctt
      901 cttaaacatt gtgaacttta aagttgaaac agaccacact tctcagacca ctttgaccaa
      961 gagctacctg tctaacagaa ccaactccag ggtgcagagc tttggaggga ttcccttcta
     1021 tccaggaatt accttagaaa cctggcagaa gggcatcact aaccacctag tggcaataga
     1081 ccgagctggc ttgcctctgc atttcttcat taagcctgac aaactgcctg gtttgccggg
     1141 tcccttggtg aaaaagctgt cgaagacagt ggaaactgct gtgagacatt attacacatt
     1201 taacactcac cctggctgca cgaatgtcga ctcccccaac ttcaattttc aagccaatat
     1261 ggaagatgat tcgtgtgatg cgaaagtaac caacttcacc tttggtggac tttatcagga
     1321 atgcactgaa ctgtcaggtg atgctctttg ccaaaacctg gagcagaaga atctgcttac
     1381 tggtgatttc tcttgtccct ctggctacac ccctgtccat ctgctgtctc agacccatga
     1441 ggagggttac agtcgcctgg aatgtaaaaa gaaatgcacc ctcaagattt tctgtaagac
     1501 agtgtgtgaa gatgtgttcc gagtggccaa agctcaattc agggcttatt ggtgcgtagc
     1561 cactggccaa gtacctgaca actcaggact tctctttgga ggactcttca ctgacaagag
     1621 catcaaccca atgacaaatg cacagtcatg cccagctggc tacattccac tgaacctgtt
     1681 tgaaagcctt aaggtatgtg tgtccctgga ttatgagttg gggtacaagt tttcagttcc
     1741 ctttggtggg ttcttcagct gtataatggg gaaccccctg gttaattctg atacagccaa
     1801 agacatagga gcaccatctc tgaaaaaatg tcctgggggc ttcagccaac acctagctgt
     1861 tatcagtgat ggatgccaag tgtcctactg tgtcaaggct ggaatcttta caggagggtc
     1921 cctgctccct gtcaggcttc caccttacac caagccaccc ctcatgagcc aagttgccac
     1981 caacactgtc atagtgacca gtagtgagac tgccagatcc tggattaagg atcctcagac
     2041 caaccagtgg aagctgggag agcctctgga gcttcataga gccatgactg tcatccatgg
     2101 ggacggtaat ggaatgtcag gaggggaagc tgctggagtc acgttgggag tcatcatagc
     2161 actaggaatt gtcattacct tggccatcta cagtactcgg aagtacaaga aagagaagga
     2221 ataccaggaa atcgaggagc aggagagttt ggttggaagc tttgcaacag atgcatcagc
     2281 ccctaatgga gaacaggatc catgtccagc ttaattgtct ccagaggaaa cagtttccag
     2341 ccgcagtttc aagtacatct ttctctttgt tttctctact tctcccttcc taagtactga
     2401 agtgaccctt gcaataggaa gaaagcaggc attacatccc tggaagtatt tgtttagacc
     2461 tctgcaccag gaaattaaag gagccatttt aatgggtatg gggaggggta tgattcatct
     2521 ggaaaaagct ggactgggac tgagccccgg gatgtgtgtg tgtgtgtgtg tgtgtgtgtg
     2581 tgtgtgtgtg tgtgtgtcca ctctcatagt tgtcattatc tgaatgcatg tgtgtaaaaa
     2641 gagattagat ttggagattg tgcatttagt ctgaatttga aaggctttcc agtttgtgag
     2701 ctgaaaaggt ttgtgctcta agtacattac ttcttttatt ttgttttcaa agaagccctc
     2761 tggatttcaa tcttgaggca aatgtgtccc tgaaatgcca caggaggcct acatgttccc
     2821 tactctttag ctttaaggac ttggtgttga ttgatagtac ccttccccct gagtcagctc
     2881 ccaggcatgt gaaggagagg cgggtggggt ttatacaaat tccatttccc aactttctca
     2941 cagctaatct agattagtct tgggcttctg aacagtgcaa acaagtcctc agggaaaaga
     3001 atatgtaact tataattttc tgttaggggg tgaaggatga agaaacagta aggaaacaaa
     3061 caataaattg aggataaaaa ctggggttct ggagacgatg cgagtaatcc aggaaagttt
     3121 gaactctcag atctgtcagt taacagcgtt ataaatgaag aactgaacag agctctggtt
     3181 tggacagatc aaacagtatg gtttcatgtt tatgtgtcaa aaatataact ccttcctctc
     3241 tgtggatgtc tttatccttg tcttctcacc ccagccgagg atgcagagag atgcacacaa
     3301 acaccctgta ttgcaacaag aagcatttat tttcctaggg gcgaaactgg gcttaaacgc
     3361 tagttcctcc ctaaccctaa acgtacgggg aaagtgcggt cttctgtctg ctaggatttg
     3421 ctgtttcaca ttgctaggaa gaggactgag gtgattaaaa acgaaacaag gttagctgga
     3481 aaaggctgtg acatgtgttt cagatccgcg atggcttcca tgggggcgtc cagcaggctg
     3541 catgattgac acctagcaat agttgggggt ttgctcaatt ttcacctctg agaatttaat
     3601 tctctacaac atgaaaattc ctccactgct gtcttagaag ttatgtatct attcaccaca
     3661 gcacatcttc agtgagctat cttctatgaa agacgatgtg ttctctcttc aatatttaca
     3721 atcaagggcc tgtgcacaca gtaggcccac ctgaagagct ccaagtgtct caatctctgg
     3781 gaatgggagt aaattactca ttctttttca gttgagtaat ttatgagaga aatgaatgac
     3841 tgaatttgaa ctctgcccta ccaactgccc agccaagggg gttgatgtat aacctctcca
     3901 tcaaacctgc ttcttgaaca ataaaatgtt ctccttaacc tttgtatgct ttatgggttt
     3961 ggggtgcatt ttaatgtttc attgaagaca atcacatttc taaacatact ggcttcattt
     4021 tgtaaattct ttaagaatta ctaagtctgc aaaacaaggc acagcagaag acttccagac
     4081 ctttaatgca gaaacttcct gttaaagtgt ctaatatttg tcccataaat atgtagctct
     4141 actaacaaaa atgtgtataa atattcatta aagtgtgtac ttagcaaaaa aaaaaaaaaa
     4201 aa
//
