LOCUS       NM_001107997            2781 bp    mRNA    linear   ROD 28-MAR-2024
DEFINITION  Rattus norvegicus membrane metallo-endopeptidase-like 1 (Mmel1),
            mRNA.
ACCESSION   NM_001107997 XM_001077229 XM_006239567 XM_233712
VERSION     NM_001107997.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Whyteside,A.R. and Turner,A.J.
  TITLE     Human neprilysin-2 (NEP2) and NEP display distinct subcellular
            localisations and substrate preferences
  JOURNAL   FEBS Lett 582 (16), 2382-2386 (2008)
   PUBMED   18539150
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Voisin,S. and Ouimet,T.
  TITLE     The ultimate tryptophan residue of neprilysin 2 is not involved in
            protein maturation and enzymatic activity
  JOURNAL   Biochem Biophys Res Commun 335 (2), 356-360 (2005)
   PUBMED   16081046
  REMARK    GeneRIF: Modification of the structure of the C-terminal region
            will either impair substrate hydrolysis or completely abolish the
            secretion and enzymatic activity of the secreted isoform of rat
            NEP2.
REFERENCE   3  (bases 1 to 2781)
  AUTHORS   Voisin,S., Rognan,D., Gros,C. and Ouimet,T.
  TITLE     A three-dimensional model of the neprilysin 2 active site based on
            the X-ray structure of neprilysin. Identification of residues
            involved in substrate hydrolysis and inhibitor binding of
            neprilysin 2
  JOURNAL   J Biol Chem 279 (44), 46172-46181 (2004)
   PUBMED   15294904
  REMARK    GeneRIF: A three-dimensional model of the neprilysin 2 active site
            based on the X-ray structure of neprilysin.
REFERENCE   4  (bases 1 to 2781)
  AUTHORS   Facchinetti,P., Rose,C., Schwartz,J.C. and Ouimet,T.
  TITLE     Ontogeny, regional and cellular distribution of the novel
            metalloprotease neprilysin 2 in the rat: a comparison with
            neprilysin and endothelin-converting enzyme-1
  JOURNAL   Neuroscience 118 (3), 627-639 (2003)
   PUBMED   12710972
REFERENCE   5  (bases 1 to 2781)
  AUTHORS   Rose,C., Voisin,S., Gros,C., Schwartz,J.C. and Ouimet,T.
  TITLE     Cell-specific activity of neprilysin 2 isoforms and enzymic
            specificity compared with neprilysin
  JOURNAL   Biochem J 363 (Pt 3), 697-705 (2002)
   PUBMED   11964170
REFERENCE   6  (bases 1 to 2781)
  AUTHORS   Ouimet,T., Facchinetti,P., Rose,C., Bonhomme,M.C., Gros,C. and
            Schwartz,J.C.
  TITLE     Neprilysin II: A putative novel metalloprotease and its isoforms in
            CNS and testis
  JOURNAL   Biochem Biophys Res Commun 271 (3), 565-570 (2000)
   PUBMED   10814502
  REMARK    Erratum:[Biochem Biophys Res Commun 2000 Aug 18;275(1):247. Tanja,
            O [corrected to Ouimet, T]]
REFERENCE   7  (bases 1 to 2781)
  AUTHORS   Ikeda,K., Emoto,N., Raharjo,S.B., Nurhantari,Y., Saiki,K.,
            Yokoyama,M. and Matsuo,M.
  TITLE     Molecular identification and characterization of novel
            membrane-bound metalloprotease, the soluble secreted form of which
            hydrolyzes a variety of vasoactive peptides
  JOURNAL   J Biol Chem 274 (45), 32469-32477 (1999)
   PUBMED   10542292
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1 and BC161870.1.
            
            On or before Aug 2, 2021 this sequence version replaced
            XM_006239567.4, NM_001107997.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360181.574051.1,
                                           SRR26643293.31886.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-60                JAXUCZ010000005.1  170713629-170713688
            61-886              BC161870.1         1-826
            887-890             JAXUCZ010000005.1  170734814-170734817
            891-2781            BC161870.1         827-2717
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..2781
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /note="membrane metallo-endopeptidase-like 1"
                     /db_xref="GeneID:313755"
                     /db_xref="RGD:1309299"
     exon            1..139
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            140..299
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     CDS             149..2473
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /EC_number="3.4.24.11"
                     /note="neprilysin 2; NL2; NEP2(m); neprilysin II; mel
                     transforming oncogene-like 1"
                     /codon_start=1
                     /product="membrane metallo-endopeptidase-like 1"
                     /protein_id="NP_001101467.2"
                     /db_xref="GeneID:313755"
                     /db_xref="RGD:1309299"
                     /translation="MGKSESSVGMMERADNCGRRRLGFVECGLLVLLTLLLMGAIVTL
                     GVFYSIGKQLPLLNSLLHVSRHERTVVKRVLRDSSQKSDICTTPSCVIAAARILQNMD
                     QSKKPCDNFYQYACGGWLRHHVIPETNSRYSVFDILRDELEVILKGVLEDSSVQHRPA
                     VEKAKTLYRSCMNQSVIEKRDSEPLLNVLDMIGGWPVAMDKWNETMGPKWELERQLAV
                     LNSQFNRRVLIDLFIWNDDQNSSRHVIYIDQPTLGMPSREYYFKEDSHRVREAYLQFM
                     TSVATMLRRDLNLPGETDLVQEEMAQVLHLETHLANATVPQEKRHDVTALYHRMGLEE
                     LQERFGLKGFNWTLFIQNVLSSVQVELLPNEEVVVYGIPYLENLEEIIDVFPAQTLQN
                     YLVWRLVLDRIGSLSQRFKEARVDYRKALYGTTMEEVRWRECVSYVNSNMESAVGSLY
                     IKRAFSKDSKSIVSELIEKIRSVFVDNLDELNWMDEESKKKAQEKALNIREQIGYPDY
                     ILEDNNRHLDEEYSSLTFSEDLYFENGLQNLKNNAQRSLKKLREKVDQNLWIIGAAVV
                     NAFYSPNRNLIVFPAGILQPPFFSKDQPQALNFGGIGMVIGHEITHGFDDNGRNFDKN
                     GNMLDWWSNFSARHFRQQSQCMIYQYSNFSWELADNQNVNGFSTLGENIADNGGVRQA
                     YKAYLQWLAEGGRDQRLPGLNLTYAQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLK
                     YRVLGSLQNLPGFSEAFHCPRGSPMHPMNRCRIW"
     exon            300..368
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            369..428
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            429..590
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            591..671
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            672..767
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            768..886
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            887..949
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1084
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1174
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1311
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1405
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1534
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1633
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1717
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1821
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1822..1880
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..2000
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2001..2134
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2135..2200
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2296
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2297..2373
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
     exon            2374..2781
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEPII"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agctccatgt gacctcacag ccagctcggc ctgaccgctg ctctactcca cccagagcct
       61 accttggcca gctcacccca gctctgcgag gtcccaaact agcctatagg acttgccaag
      121 tggcagctga cagcactaga accggctgat ggggaagtcg gagagctcag tggggatgat
      181 ggagagagcg gacaactgtg ggaggaggcg cctaggcttc gtggagtgtg ggctgctggt
      241 actgctgaca ctgctgttga tgggagccat agtgactctg ggtgtcttct acagcatagg
      301 gaagcagctg cccctcttaa atagcctgct gcacgtctcc cggcatgaga ggacggttgt
      361 aaaacgagtc ctcagagatt catcgcagaa gagtgacatc tgtactaccc caagctgcgt
      421 gatagcagct gccagaatcc tccagaacat ggaccagtca aagaaaccct gcgacaactt
      481 ctatcagtat gcttgcggag gctggctacg gcaccatgtg atccccgaga ccaactccag
      541 atacagcgtc tttgacatcc ttcgggatga gctggaggtc atcctcaaag gggtgctgga
      601 ggattcctct gtccagcacc gcccagctgt ggagaaggcc aagacactgt accgctcctg
      661 catgaaccag agtgtgatag agaagagaga ctctgagccc ctgctgaacg tcttagatat
      721 gataggaggt tggcctgtag ccatggacaa gtggaatgag accatgggcc ccaagtggga
      781 actggagcgg cagttggctg tgttgaactc gcagttcaac aggcgcgtcc tcatcgacct
      841 cttcatctgg aatgatgacc agaactccag ccggcacgtc atctacatag accagcccac
      901 cttgggcatg ccctcccggg agtactattt caaggaagac agccaccggg tacgggaagc
      961 ctacctgcag ttcatgacat cagtggccac tatgctgagg agagacctga acctgcccgg
     1021 ggagaccgat ttggtgcagg aggaaatggc acaggtgctg catctggaga cacatctggc
     1081 caacgccacg gtcccccagg agaaaaggca tgatgtcacc gccctgtatc accgaatggg
     1141 cctggaggag ctgcaggaaa ggtttggtct gaaggggttt aactggactc tcttcataca
     1201 aaacgtgctg tcttctgtgc aagttgagct gctcccgaat gaggaggtgg tggtctatgg
     1261 catcccctac ctggagaatc ttgaggagat cattgacgtc ttcccagcac agaccttgca
     1321 aaactacctg gtgtggcgcc tggtgctaga tcgcatcggc agcctgagcc agagattcaa
     1381 agaagcgcgt gtggactacc gcaaggcgct gtacggtaca accatggagg aagtacgctg
     1441 gcgggagtgt gtcagctatg tcaacagcaa catggagagt gccgtgggct ccctctacat
     1501 caagcgggcc ttctccaagg acagcaagag catagtcagt gagcttatcg agaagatacg
     1561 gtccgtgttt gtggataacc tggacgagtt gaactggatg gatgaggaat ccaagaaaaa
     1621 ggcccaggaa aaggccttga atatccggga acagatcggc taccctgact acattttgga
     1681 agacaataac agacacctgg atgaggaata ctccagtctg actttctcag aggacctgta
     1741 ttttgagaac gggcttcaga acctcaagaa caatgcccaa aggagcctca agaaacttcg
     1801 ggaaaaggtg gaccagaatc tctggatcat tggggctgca gtggtcaatg cattctactc
     1861 cccaaacaga aacctgatcg tctttccagc ggggatcctc cagccaccct tcttcagcaa
     1921 ggaccaacca caggccttga atttcggggg catcgggatg gtgattggac acgagatcac
     1981 acacggcttt gatgataacg gtcggaactt tgacaagaat ggcaacatgc tggactggtg
     2041 gagcaacttc tcggcccggc acttccgaca gcagtcacag tgtatgattt atcagtacag
     2101 caacttctct tgggaactag cagacaacca gaatgtgaac ggattcagca ccctcgggga
     2161 gaacatcgcc gacaacggcg gtgtgcggca ggcatacaag gcttacctac agtggctagc
     2221 tgaaggcggc agagaccaga gactgccggg actgaacctg acctatgctc agcttttctt
     2281 cattaactat gcccaggtgt ggtgtgggtc ctacaggccg gagttcgcca tccagtccat
     2341 caagacagat gtccacagtc ctcttaagta cagggtgctg ggctcactac agaacctacc
     2401 aggcttctct gaggcgttcc actgcccacg aggcagcccc atgcacccta tgaatcgatg
     2461 tcgcatctgg tagccaaggc tgagctatgc tgcggcccac gccccgccac ccagaggctt
     2521 cgtgaatggt gtagccggca tagatgtgca ggttgttgcc tgaaggccac tggagccacc
     2581 agccagccct ccgcgcccag cctagagggc agccacccgc ccacatctgg gatgagtggt
     2641 ggtgcctggt cctgcgcctt ttccggccag tgagggtcag cggcccggta ggagcagtca
     2701 gctgtccccc accctcttca tagtgtgtgg ctaaatgtcc tcgagcttca gacttgagct
     2761 aagtaaacgc ttcaaagaag g
//
