LOCUS       NM_012608               3493 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus membrane metallo-endopeptidase (Mme), mRNA.
ACCESSION   NM_012608
VERSION     NM_012608.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Vasilev,D., Dubrovskaya,N.M. and Nalivaeva,N.N.
  TITLE     Caspase Inhibition Restores NEP Expression and Rescues Olfactory
            Deficit in Rats Caused by Prenatal Hypoxia
  JOURNAL   J Mol Neurosci 72 (7), 1516-1526 (2022)
   PUBMED   35344141
  REMARK    GeneRIF: Caspase Inhibition Restores NEP Expression and Rescues
            Olfactory Deficit in Rats Caused by Prenatal Hypoxia.
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Vasilev,D.S., Dubrovskaya,N.M., Zhuravin,I.A. and Nalivaeva,N.N.
  TITLE     Developmental Profile of Brain Neprilysin Expression Correlates
            with Olfactory Behaviour of Rats
  JOURNAL   J Mol Neurosci 71 (9), 1772-1785 (2021)
   PUBMED   33433852
  REMARK    GeneRIF: Developmental Profile of Brain Neprilysin Expression
            Correlates with Olfactory Behaviour of Rats.
REFERENCE   3  (bases 1 to 3493)
  AUTHORS   Dominguez-Vias,G., Aretxaga-Maza,G., Prieto,I., Segarra,A.B.,
            Luna,J.D., Gasparo,M. and Ramirez-Sanchez,M.
  TITLE     Light-dark influence on enkephalinase activity in hypothalamus and
            pituitary
  JOURNAL   Neuro Endocrinol Lett 39 (4), 277-280 (2018)
   PUBMED   30531704
  REMARK    GeneRIF: In standard light/dark conditions, the results
            demonstrated an opposite daily rhythm in hypothalamus and
            pituitary. While the highest levels of AlaAP or enkephalinase
            activities were measured in hypothalamus during the dark period,
            they were the highest in the pituitary during the light one.
REFERENCE   4  (bases 1 to 3493)
  AUTHORS   de Oliveira,R.W., Julian,G.S., Perry,J.C., Tufik,S. and Chagas,J.R.
  TITLE     Chronic intermittent hypoxia induces changes on the expression and
            activity of neprilysin (EC 3.4.24.11) in the brain of rats
  JOURNAL   Neurosci Lett 678, 43-47 (2018)
   PUBMED   29702204
  REMARK    GeneRIF: Study shows that chronic intermittent hypoxia (CIH)
            induces changes on the expression and activity of neprilysin (NEP)
            in the brain of rats. Data show that CIH protocol increases the
            expression and activity of NEP selectively in the temporal cortex.
REFERENCE   5  (bases 1 to 3493)
  AUTHORS   Liu,C.X., Tan,L., Sun,F.R., Zhang,W., Miao,D., Tan,M.S., Wan,Y.,
            Tan,C.C., Yu,J.T. and Tan,L.
  TITLE     The Association of MME microRNA Binding Site Polymorphism with the
            Risk of Late Onset Alzheimer's Disease in Northern Han Chinese
  JOURNAL   Curr Neurovasc Res 14 (2), 90-95 (2017)
   PUBMED   28294061
REFERENCE   6  (bases 1 to 3493)
  AUTHORS   Dutriez,I., Sales,N., Fournie-Zaluski,M.C. and Roques,B.P.
  TITLE     Pre- and post-natal ontogeny of neutral endopeptidase 24-11
            ('enkephalinase') studied by in vitro autoradiography in the rat
  JOURNAL   Experientia 48 (3), 290-300 (1992)
   PUBMED   1547865
REFERENCE   7  (bases 1 to 3493)
  AUTHORS   Yandle,T.G., Brennan,S.O., Espiner,E.A., Nicholls,M.G. and
            Richards,A.M.
  TITLE     Endopeptidase-24.11 in human plasma degrades atrial natriuretic
            factor (ANF) to ANF(99-105/106-126)
  JOURNAL   Peptides 10 (4), 891-894 (1989)
   PUBMED   2531377
REFERENCE   8  (bases 1 to 3493)
  AUTHORS   Bateman,R.C. Jr., Jackson,D., Slaughter,C.A., Unnithan,S.,
            Chai,Y.G., Moomaw,C. and Hersh,L.B.
  TITLE     Identification of the active-site arginine in rat neutral
            endopeptidase 24.11 (enkephalinase) as arginine 102 and analysis of
            a glutamine 102 mutant
  JOURNAL   J Biol Chem 264 (11), 6151-6157 (1989)
   PUBMED   2703483
REFERENCE   9  (bases 1 to 3493)
  AUTHORS   Shipp,M.A., Vijayaraghavan,J., Schmidt,E.V., Masteller,E.L.,
            D'Adamio,L., Hersh,L.B. and Reinherz,E.L.
  TITLE     Common acute lymphoblastic leukemia antigen (CALLA) is active
            neutral endopeptidase 24.11 ('enkephalinase'): direct evidence by
            cDNA transfection analysis
  JOURNAL   Proc Natl Acad Sci U S A 86 (1), 297-301 (1989)
   PUBMED   2521388
REFERENCE   10 (bases 1 to 3493)
  AUTHORS   Marcais-Collado,H., Uchida,G., Costentin,J., Schwartz,J.C. and
            Lecomte,J.M.
  TITLE     Naloxone-reversible antidiarrheal effects of enkephalinase
            inhibitors
  JOURNAL   Eur J Pharmacol 144 (2), 125-132 (1987)
   PUBMED   3481337
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC085753.1.
            
            On Nov 15, 2004 this sequence version replaced NM_012608.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC085753.1, SRR8487227.322012.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q31"
     gene            1..3493
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /note="membrane metallo-endopeptidase"
                     /db_xref="GeneID:24590"
                     /db_xref="RGD:3098"
     exon            1..118
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            119..288
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     CDS             129..2381
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /EC_number="3.4.24.11"
                     /note="membrane metallo-endopeptidase (neutral
                     endopeptidase/enkephalinase); atriopeptidase; neutral
                     endopeptidase 24.11; skin fibroblast elastase"
                     /codon_start=1
                     /product="neprilysin"
                     /protein_id="NP_036740.1"
                     /db_xref="GeneID:24590"
                     /db_xref="RGD:3098"
                     /translation="MGRSESQMDITDINAPKPKKKQRWTPLEISLSVLVLLLTIIAVT
                     MIALYATYDDGICKSSDCIKSAARLIQNMDASAEPCTDFFKYACGGWLKRNVIPETSS
                     RYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLTL
                     LPDIYGWPVASQNWEQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIH
                     FDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQRLPIDENQLSLEMNKV
                     MELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEINGKPFSWSNFTNEIMSTV
                     NINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRN
                     AFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREV
                     FIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYKEEEYF
                     ENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFS
                     ARQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVY
                     QYGNFTWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNH
                     KQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSY
                     MNPERKCRVW"
     exon            289..324
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            325..486
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            487..567
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            568..663
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            664..782
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            783..848
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            849..983
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1085
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1222
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1316
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1445
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1544
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1625
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..1729
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1730..1788
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1789..1908
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1909..2042
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2043..2108
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2109..2204
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2281
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..3465
                     /gene="Mme"
                     /gene_synonym="CD10; Nep; SFE"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcccaggagt tctgtttggt gaccgagagc agcgctgcca aattgcaccg gggttcattt
       61 gggggctggg aattggccat tccgctgtac agacactgtt tttattttaa aaagcaagat
      121 tttaggtgat gggaagatca gaaagtcaga tggatattac tgatatcaat gctccaaagc
      181 cgaagaagaa acagcgatgg actccactgg agatcagcct ttctgtgctc gtcttgctcc
      241 tgactatcat agctgtgaca atgattgctc tctatgcaac ctatgatgat ggtatttgca
      301 aatcatcaga ctgcataaaa tcagctgctc gactgatcca gaacatggat gcctctgctg
      361 agccatgtac ggacttcttc aaatatgctt gtggaggctg gttgaaacgc aatgtcatcc
      421 ctgagaccag ttcccgatac agtaattttg acattctaag agatgaacta gaagtcattt
      481 tgaaagatgt ccttcaagaa cccaaaactg aggacatagt agcagtgcag aaagcaaaaa
      541 ctttgtacag atcatgtata aatgaatctg ctattgatag cagaggtggg caacctctgc
      601 tcacactgtt accagatata tatgggtggc cagtagcatc acaaaactgg gaacaaacat
      661 atggtacttc ttggacagct gagaaatcta ttgcacaact gaattctaaa tatgggaaaa
      721 aggtcctcat taattttttt gttggcactg atgataagaa ttctacccag catataattc
      781 attttgacca gcctcgactt ggcctccctt ccagagacta ctatgagtgt acaggaatat
      841 ataaagaggc ttgcacagca tatgtggatt ttatgatttc tgtggccaga ctgattcgtc
      901 aggaacaaag attgcctatt gatgaaaacc agctctcttt ggaaatgaat aaagttatgg
      961 aattggaaaa agaaattgcc aatgccacaa ctaaaccaga agaccgaaat gacccaatgc
     1021 tgctttataa caaaatgaca ttggccaagc tccaaaataa cttctctctg gagatcaatg
     1081 ggaagccatt cagctggtca aatttcacaa atgaaatcat gtcaactgtg aatattaata
     1141 ttcaaaatga ggaagaagtg gttgtttatg ctccagaata tttaaccaaa cttaagccta
     1201 ttcttaccaa atattctccc agagatcttc aaaatttaat gtcctggagg ttcataatgg
     1261 atcttgtaag cagcctcagc cgaaactaca aggagtccag aaatgctttc cgcaaggccc
     1321 tttacgggac tacatccgaa actgcaacct ggagacggtg tgccaactac gtcaatggga
     1381 acatggagaa tgctgtgggg aggctttatg tggaagcagc ttttgctgga gagagcaagc
     1441 acgtggttga agatttgatt gcacaaatcc gtgaagtttt tattcagact ttagatgacc
     1501 tcacttggat ggatgctgag acaaaaaaga aagctgaaga gaaggccctg gcaattaaag
     1561 aaaggattgg ctatcctgat gacatcatct ccaatgagaa taaactgaat aatgagtatc
     1621 ttgagttgaa ctacaaggaa gaggaatact ttgagaacat aattcaaaat ttgaaattca
     1681 gccaaagcaa gcagctaaag aagctccgag aaaaggtgga caaagatgag tggataagtg
     1741 gcgcggcggt agtcaatgca ttttattcct caggcagaaa tcagatcgtc ttccccgccg
     1801 gcattttgca gcccccattc tttagtgctc ggcagtccaa ctcattgaac tatgggggca
     1861 tcggcatggt catcggacat gaaatcacac atggctttga tgacaatggc agaaatttta
     1921 acaaagatgg agacctcgtt gactggtgga ctcagcagtc tgcaaataat ttcaaagacc
     1981 aatcccagtg tatggtgtac cagtatggaa actttacatg ggacctagca ggtggacagc
     2041 atctcaatgg aattaacaca ctaggagaaa atattgctga taatggaggg attggccaag
     2101 catacagagc ctatcagaat tatgttaaaa agaatggtga agaaaaatta ctccctggac
     2161 ttgacctcaa tcacaaacaa ctattcttct tgaactttgc ccaggtgtgg tgtggaacct
     2221 accggccaga gtatgcagtc aattccatta aaacagatgt acacagtcct ggcaatttca
     2281 ggatcattgg gactttgcag aactctgctg agtttgcgga tgcctttcat tgccgcaaga
     2341 actcatacat gaatccagaa aggaaatgtc gggtttggtg atcttcacag gaagtggagc
     2401 atccatggca ggactcgcca aagccacaga aacaggaagt cttccctcag agaacgtggg
     2461 ccccggaagt ttcttcagct tcttggggga aattcacaga gatgagcacg agctaacaaa
     2521 aatgaaatta gattattaaa accgctgtga atgaaagggg agaaaaccta cgatctagca
     2581 aatcaatcac ttcactgtgt aaataattac cttccaacgg taatattacc gttcacttct
     2641 ggttctcaca cagactgcag ctttcatgct gtctgtagag aacagtgtta acacttaaag
     2701 caggttatga cttctgatca agaggaggaa gacgctgaat acagttgggc accaaagtac
     2761 agatttgcct ctcagcactc acttttgttt gcaacattca gctccttcaa aattctccca
     2821 aagaaccccc atgcatactg tggccttcag gctcctgcag tgtggaactc attttaccat
     2881 gcataaatta ttcattcatt ccacatcatt ttagtttgag cactcttaga gcttaaacta
     2941 gagagtctga aatggttccg ccatttaccc acttgagtga tgttgagact cttcagcccc
     3001 ctacagattt ttgagcaatt tcttgctctc gctgcccctc agacttagtc ttttaaagga
     3061 tttgtagtaa tgtataaaaa acattctata tttaattatt aactacacat gaccaaataa
     3121 accattgcta taggtaatca ttgaatattg acattatatg gccaagatag atagttaaga
     3181 agatctgtaa catgatgtgc agatgaaaat ttgaaacttt ttaagcctgt aaatcatatt
     3241 gctgaaaatc ttcaaacaca aactctgggg tgagcattac cattgaacag ttgtcttgag
     3301 atgcataata gttttggttt acaaacagcg ttttgaagat taagtctggc ctcagtggct
     3361 aagaaagggc ctaactctta tctgtgttac tccatgcgtc cttgttcttc aggtttgtca
     3421 tccgatggaa atattttgat aataaattga agttatgagt ccctaaaaaa aaaaaaaaaa
     3481 aaaaaaaaaa aaa
//
