LOCUS       NM_001401077            2454 bp    mRNA    linear   ROD 23-APR-2024
DEFINITION  Rattus norvegicus mixed lineage kinase domain like pseudokinase
            (Mlkl), mRNA.
ACCESSION   NM_001401077 XM_008772572
VERSION     NM_001401077.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Marunouchi,T., Onda,S., Kurasawa,M. and Tanonaka,K.
  TITLE     Angiotensin II Is Involved in MLKL Activation During the
            Development of Heart Failure Following Myocardial Infarction in
            Rats
  JOURNAL   Biol Pharm Bull 47 (4), 809-817 (2024)
   PUBMED   38583954
  REMARK    GeneRIF: Angiotensin II Is Involved in MLKL Activation During the
            Development of Heart Failure Following Myocardial Infarction in
            Rats.
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Tang,Y., Chu,Q., Xie,G., Tan,Y., Ye,Z. and Qin,C.
  TITLE     MLKL regulates Cx43 ubiquitinational degradation and mediates
            neuronal necroptosis in ipsilateral thalamus after focal cortical
            infarction
  JOURNAL   Mol Brain 16 (1), 74 (2023)
   PUBMED   37904209
  REMARK    GeneRIF: MLKL regulates Cx43 ubiquitinational degradation and
            mediates neuronal necroptosis in ipsilateral thalamus after focal
            cortical infarction.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2454)
  AUTHORS   Ino,S., Yano,T., Kuno,A., Tanno,M., Kouzu,H., Sato,T.,
            Yamashita,T., Ohwada,W., Osanami,A., Ogawa,T., Toda,Y., Shimizu,M.
            and Miura,T.
  TITLE     Nuclear translocation of MLKL enhances necroptosis by a
            RIP1/RIP3-independent mechanism in H9c2 cardiomyoblasts
  JOURNAL   J Pharmacol Sci 151 (2), 134-143 (2023)
   PUBMED   36707179
  REMARK    GeneRIF: Nuclear translocation of MLKL enhances necroptosis by a
            RIP1/RIP3-independent mechanism in H9c2 cardiomyoblasts.
REFERENCE   4  (bases 1 to 2454)
  AUTHORS   Yuan,G., Cao,C., Cao,D., Li,B., Li,X., Li,H., Shen,H., Wang,Z. and
            Chen,G.
  TITLE     Receptor-interacting protein 3-phosphorylated Ca2+
            /calmodulin-dependent protein kinase II and mixed lineage kinase
            domain-like protein mediate intracerebral hemorrhage-induced
            neuronal necroptosis
  JOURNAL   J Neurochem 164 (1), 94-114 (2023)
   PUBMED   36424866
  REMARK    GeneRIF: Receptor-interacting protein 3-phosphorylated Ca[2+]
            /calmodulin-dependent protein kinase II and mixed lineage kinase
            domain-like protein mediate intracerebral hemorrhage-induced
            neuronal necroptosis.
REFERENCE   5  (bases 1 to 2454)
  AUTHORS   Xia,C., Shao,L., Ma,Y., Wang,X., Zhang,Y., Shi,C., Li,J., Zhang,W.,
            Li,H. and Wang,J.
  TITLE     Protective action of ultrasound-guided intraparenchymal
            transplantation of BMSCs in adriamycin nephropathy rats through the
            RIPK3/MLKL and NLRP3 pathways
  JOURNAL   Acta Histochem 123 (7), 151773 (2021)
   PUBMED   34517258
  REMARK    GeneRIF: Protective action of ultrasound-guided intraparenchymal
            transplantation of BMSCs in adriamycin nephropathy rats through the
            RIPK3/MLKL and NLRP3 pathways.
            Erratum:[Acta Histochem. 2022 Feb;124(2):151846. PMID: 35033378]
REFERENCE   6  (bases 1 to 2454)
  AUTHORS   Xie,T., Peng,W., Yan,C., Wu,J., Gong,X. and Shi,Y.
  TITLE     Structural insights into RIP3-mediated necroptotic signaling
  JOURNAL   Cell Rep 5 (1), 70-78 (2013)
   PUBMED   24095729
REFERENCE   7  (bases 1 to 2454)
  AUTHORS   Murphy,J.M., Czabotar,P.E., Hildebrand,J.M., Lucet,I.S.,
            Zhang,J.G., Alvarez-Diaz,S., Lewis,R., Lalaoui,N., Metcalf,D.,
            Webb,A.I., Young,S.N., Varghese,L.N., Tannahill,G.M.,
            Hatchell,E.C., Majewski,I.J., Okamoto,T., Dobson,R.C., Hilton,D.J.,
            Babon,J.J., Nicola,N.A., Strasser,A., Silke,J. and Alexander,W.S.
  TITLE     The pseudokinase MLKL mediates necroptosis via a molecular switch
            mechanism
  JOURNAL   Immunity 39 (3), 443-453 (2013)
   PUBMED   24012422
REFERENCE   8  (bases 1 to 2454)
  AUTHORS   Wu,J., Huang,Z., Ren,J., Zhang,Z., He,P., Li,Y., Ma,J., Chen,W.,
            Zhang,Y., Zhou,X., Yang,Z., Wu,S.Q., Chen,L. and Han,J.
  TITLE     Mlkl knockout mice demonstrate the indispensable role of Mlkl in
            necroptosis
  JOURNAL   Cell Res 23 (8), 994-1006 (2013)
   PUBMED   23835476
REFERENCE   9  (bases 1 to 2454)
  AUTHORS   Wang,Z., Jiang,H., Chen,S., Du,F. and Wang,X.
  TITLE     The mitochondrial phosphatase PGAM5 functions at the convergence
            point of multiple necrotic death pathways
  JOURNAL   Cell 148 (1-2), 228-243 (2012)
   PUBMED   22265414
REFERENCE   10 (bases 1 to 2454)
  AUTHORS   Sun,L., Wang,H., Wang,Z., He,S., Chen,S., Liao,D., Wang,L., Yan,J.,
            Liu,W., Lei,X. and Wang,X.
  TITLE     Mixed lineage kinase domain-like protein mediates necrosis
            signaling downstream of RIP3 kinase
  JOURNAL   Cell 148 (1-2), 213-227 (2012)
   PUBMED   22265413
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000019.1.
            
            On Feb 1, 2022 this sequence version replaced XM_008772572.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643429.56096.1,
                                           SRR26643299.64782.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-375               JAXUCZ010000019.1  56213484-56213858   c
            376-808             JAXUCZ010000019.1  56207316-56207748   c
            809-882             JAXUCZ010000019.1  56204364-56204437   c
            883-1066            JAXUCZ010000019.1  56200493-56200676   c
            1067-1164           JAXUCZ010000019.1  56196380-56196477   c
            1165-1300           JAXUCZ010000019.1  56191924-56192059   c
            1301-1376           JAXUCZ010000019.1  56191590-56191665   c
            1377-1528           JAXUCZ010000019.1  56190776-56190927   c
            1529-1578           JAXUCZ010000019.1  56189994-56190043   c
            1579-1719           JAXUCZ010000019.1  56189490-56189630   c
            1720-2454           JAXUCZ010000019.1  56186860-56187594   c
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..2454
                     /gene="Mlkl"
                     /note="mixed lineage kinase domain like pseudokinase"
                     /db_xref="GeneID:690743"
                     /db_xref="RGD:1592221"
     exon            1..375
                     /gene="Mlkl"
                     /inference="alignment:Splign:2.1.0"
     exon            376..808
                     /gene="Mlkl"
                     /inference="alignment:Splign:2.1.0"
     CDS             378..1772
                     /gene="Mlkl"
                     /note="rCG51567-like"
                     /codon_start=1
                     /product="mixed lineage kinase domain-like protein"
                     /protein_id="NP_001388006.1"
                     /db_xref="GeneID:690743"
                     /db_xref="RGD:1592221"
                     /translation="MDKLGQIIKLGQLIYDQCEKMKYCKRQCRRLRNRVHGLLQPLQR
                     LQAQEKKSLPSDITAALGRFEDVLKEARQQIEKFSQKSNIWKFATVGSDKILFHEVNE
                     KLRDIWEELLLLLQVDQWKSDSEVSQPASWQQEDQQDAEEDGNENMKVILVKLQISME
                     EIKEALKQCSLKPMQEVPQDFQVKEIPKEDLGYPWTKLKTNKLSTIYRGEYYKSPVTI
                     KVFNNPKAESVGIVRLTFNEEIKTMKKFDSPNILRIFGICIDQTVKPPEFSIVMEYCE
                     LGTLRELLDRDKDLTMSVRSLLVLRAARGLYRLHHSGTLHGNISSSSFLVAGGYQVKL
                     AGFELSKTQTSISRATKSTKAERFSSTAYVSPERLQDPFCKYDTKAEIYSFGIVLWEI
                     ATGKIPFEGCDSEKIYELVAENKKQEPVGQDCPELLQEIINECRAHEPSNRPSADGIL
                     ERLPAIEDSMDKKL"
     exon            809..882
                     /gene="Mlkl"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1066
                     /gene="Mlkl"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1164
                     /gene="Mlkl"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1300
                     /gene="Mlkl"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1376
                     /gene="Mlkl"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1528
                     /gene="Mlkl"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1578
                     /gene="Mlkl"
                     /inference="alignment:Splign:2.1.0"
     exon            1579..1719
                     /gene="Mlkl"
                     /inference="alignment:Splign:2.1.0"
     exon            1720..2454
                     /gene="Mlkl"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggaagaaga aacagctgtt ggttttgagg gctcggctgg ggcattgctg tgttaaccca
       61 aatttgactt tacacgagaa ctgtggccgg tgtagaattc ctccgggatg cgggagcatt
      121 aaggctgcac caagggaagg ggctcaggag accggggacc tgtcctggtc tggggtgcat
      181 tgtcagcgag gaagaagatc acagtggcca gtgggagact gatcctactg gaacactgga
      241 acggagcgca ggatagacca agaccagcgc ccctcggaaa aaccgggctg agtcctcaga
      301 ctccagggag ccatagcgca gcaggaggaa gcaggagaat cacggaatca cagataggac
      361 ttcctggaac tcggggtatg gataaattgg gacagatcat caagttaggc cagctcatct
      421 atgaccagtg tgaaaagatg aaatactgca aaagacagtg ccggcgtcta agaaaccgtg
      481 tgcatggcct gctacagcct ctccagaggc tccaggccca agaaaagaag agcctcccca
      541 gtgacattac tgctgccctg ggccgttttg aggacgtcct aaaagaagct agacagcaga
      601 tagaaaagtt cagtcagaag tccaatattt ggaagtttgc tactgtgggc agtgataaga
      661 tcctcttcca tgaagtgaat gagaagctga gagacatctg ggaggagctc ttgctgctgc
      721 ttcaggttga tcaatggaag tctgattccg aggtcagcca accagcatcc tggcagcagg
      781 aagatcaaca ggacgcagag gaagatggaa atgaaaatat gaaagttatt ttggtgaagc
      841 tgcaaatcag catggaagaa atcaaagagg ccctgaagca atgctcacta aaacccatgc
      901 aggaggtccc acaagatttc caagtcaagg agattccgaa ggaagatctt ggatatccgt
      961 ggaccaaact gaagacaaat aaactgagca cgatttatag aggagagtat tacaaatctc
     1021 cagtcaccat caaagtattc aacaacccca aggccgaaag tgttggaata gtgaggctca
     1081 ctttcaatga ggagatcaaa accatgaaga aatttgattc tcccaacatc ctgcgtatat
     1141 ttgggatttg catcgatcaa acagtgaagc cccccgaatt ctccattgtc atggagtact
     1201 gtgaactcgg aaccctgagg gaactgctgg atagagacaa ggacctcacc atgagtgtgc
     1261 gcagcctcct agtcctgagg gcagctagag gcttgtacag gttacaccac tcgggaacac
     1321 tccacggaaa catcagcagc tccagcttcc tggtagctgg aggctaccaa gtaaagcttg
     1381 caggatttga gctaagcaaa acacagactt ctataagccg ggcaacaaag agcactaaag
     1441 cagagagatt cagttcgaca gcatatgtct cccccgagag actgcaagat ccattttgca
     1501 aatatgacac aaaagctgaa atatatagct ttggaattgt gctctgggaa attgccactg
     1561 gaaagatccc gtttgaaggc tgtgattctg agaagatcta tgagctggtg gctgagaaca
     1621 agaagcagga gccagtgggt caggattgcc ccgagttgtt gcaggagatc attaatgagt
     1681 gccgggccca tgaaccctcc aatcggccct ctgcggatgg aatcttggag agactgcctg
     1741 ccattgaaga ctccatggac aaaaagctgt aagagggaca aactggacat gtgtccttcc
     1801 cattggtcag cagcatcaac ttattgctgg gtgcaaaaat ctttgttctc ccttggatgc
     1861 ttcttttttc ttcctttctt tttcttgaga tgggatctca ccaagaagcc cctgcaggcc
     1921 tggagcttgc tatgtagagc aggctggccc aaacttccag ttttgcctcc tgaatgctgg
     1981 gattaagggc acagactgcc aaactgtcat gcttcttatt tgttagaaat agaaaacaat
     2041 tgcatgggac tggaaacatg gctctgtagt taagagcact ggctgttctt ccagaagacc
     2101 cgggttcaat tcccagcaac cacatggtgg ctcacaacca tctgtaactc ctgttccagg
     2161 gatccaacac tctcacacag acatacatgc aggcaaaaaa ccaatgtaca taaaataaaa
     2221 ataaaattaa tcccccccaa ttttttttaa aactaaacaa ttctatctca gtgtggtagt
     2281 gcacaccttt gatcccagtg cttggaaggc agaggcaggt gggtctctgt ttgaggccag
     2341 cctggtctac agatcaagtt ctgtactatg taagacctgt ctcaaaaaaa ataaaacaaa
     2401 aaattgacag ccatatttct ttctttaatt aattattttt ggggggtagt gagt
//
