LOCUS       NM_031053               2589 bp    mRNA    linear   ROD 05-DEC-2024
DEFINITION  Rattus norvegicus mutL homolog 1 (Mlh1), mRNA.
ACCESSION   NM_031053 XM_346837
VERSION     NM_031053.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Wu,X.Y., Chen,H.C., Li,W.W., Yan,J.D. and Lv,R.Y.
  TITLE     DNMT1 promotes cell proliferation via methylating hMLH1 and hMSH2
            promoters in EGFR-mutated non-small cell lung cancer
  JOURNAL   J Biochem 168 (2), 151-157 (2020)
   PUBMED   32211850
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Kowluru,R.A. and Mohammad,G.
  TITLE     Epigenetics and Mitochondrial Stability in the Metabolic Memory
            Phenomenon Associated with Continued Progression of Diabetic
            Retinopathy
  JOURNAL   Sci Rep 10 (1), 6655 (2020)
   PUBMED   32313015
  REMARK    GeneRIF: Epigenetics and Mitochondrial Stability in the Metabolic
            Memory Phenomenon Associated with Continued Progression of Diabetic
            Retinopathy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2589)
  AUTHORS   Maletzki,C., Huehns,M., Bauer,I., Ripperger,T., Mork,M.M.,
            Vilar,E., Klocking,S., Zettl,H., Prall,F. and Linnebacher,M.
  TITLE     Frameshift mutational target gene analysis identifies similarities
            and differences in constitutional mismatch repair-deficiency and
            Lynch syndrome
  JOURNAL   Mol Carcinog 56 (7), 1753-1764 (2017)
   PUBMED   28218421
REFERENCE   4  (bases 1 to 2589)
  AUTHORS   Huang,S.C., Huang,S.F., Chen,Y.T., Chang,Y., Chiu,Y.T., Chang,I.C.,
            Wu,H.I. and Chen,J.S.
  TITLE     Overexpression of MutL homolog 1 and MutS homolog 2 proteins have
            reversed prognostic implications for stage I-II colon cancer
            patients
  JOURNAL   Biomed J 40 (1), 39-48 (2017)
   PUBMED   28411881
REFERENCE   5  (bases 1 to 2589)
  AUTHORS   Finsterbusch,F., Ravindranathan,R., Dereli,I., Stanzione,M.,
            Trankner,D. and Toth,A.
  TITLE     Alignment of Homologous Chromosomes and Effective Repair of
            Programmed DNA Double-Strand Breaks during Mouse Meiosis Require
            the Minichromosome Maintenance Domain Containing 2 (MCMDC2) Protein
  JOURNAL   PLoS Genet 12 (10), e1006393 (2016)
   PUBMED   27760146
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2589)
  AUTHORS   Prolla,T.A., Baker,S.M., Harris,A.C., Tsao,J.L., Yao,X.,
            Bronner,C.E., Zheng,B., Gordon,M., Reneker,J., Arnheim,N.,
            Shibata,D., Bradley,A. and Liskay,R.M.
  TITLE     Tumour susceptibility and spontaneous mutation in mice deficient in
            Mlh1, Pms1 and Pms2 DNA mismatch repair
  JOURNAL   Nat Genet 18 (3), 276-279 (1998)
   PUBMED   9500552
REFERENCE   7  (bases 1 to 2589)
  AUTHORS   Baker,S.M., Plug,A.W., Prolla,T.A., Bronner,C.E., Harris,A.C.,
            Yao,X., Christie,D.M., Monell,C., Arnheim,N., Bradley,A., Ashley,T.
            and Liskay,R.M.
  TITLE     Involvement of mouse Mlh1 in DNA mismatch repair and meiotic
            crossing over
  JOURNAL   Nat Genet 13 (3), 336-342 (1996)
   PUBMED   8673133
REFERENCE   8  (bases 1 to 2589)
  AUTHORS   Edelmann,W., Cohen,P.E., Kane,M., Lau,K., Morrow,B., Bennett,S.,
            Umar,A., Kunkel,T., Cattoretti,G., Chaganti,R., Pollard,J.W.,
            Kolodner,R.D. and Kucherlapati,R.
  TITLE     Meiotic pachytene arrest in MLH1-deficient mice
  JOURNAL   Cell 85 (7), 1125-1134 (1996)
   PUBMED   8674118
REFERENCE   9  (bases 1 to 2589)
  AUTHORS   Zechmeister-Machhart,M., Hufnagl,P., Uhrin,P., Xu,J., Geiger,M. and
            Binder,B.R.
  TITLE     Molecular cloning and tissue distribution of mouse protein C
            inhibitor (PCI)
  JOURNAL   Immunopharmacology 32 (1-3), 96-98 (1996)
   PUBMED   8796278
REFERENCE   10 (bases 1 to 2589)
  AUTHORS   Sasaki,S., Horii,A., Shimada,M., Han,H.J., Yanagisawa,A., Muto,T.
            and Nakamura,Y.
  TITLE     Somatic mutations of a human mismatch repair gene, hMLH1, in tumors
            from patients with multiple primary cancers
  JOURNAL   Hum Mutat 7 (3), 275-278 (1996)
   PUBMED   8829664
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Nov 25, 2020 this sequence version replaced NM_031053.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments. I kept the dAUG as it
            is better conserved.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U80054.1, SRR26643287.21476.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-185               JAXUCZ010000008.1  120111827-120112011 c
            186-276             JAXUCZ010000008.1  120109463-120109553 c
            277-375             JAXUCZ010000008.1  120107346-120107444 c
            376-449             JAXUCZ010000008.1  120104044-120104117 c
            450-522             JAXUCZ010000008.1  120100713-120100785 c
            523-614             JAXUCZ010000008.1  120099575-120099666 c
            615-657             JAXUCZ010000008.1  120097431-120097473 c
            658-746             JAXUCZ010000008.1  120096995-120097083 c
            747-859             JAXUCZ010000008.1  120095213-120095325 c
            860-953             JAXUCZ010000008.1  120092316-120092409 c
            954-1107            JAXUCZ010000008.1  120090434-120090587 c
            1108-1484           JAXUCZ010000008.1  120087366-120087742 c
            1485-1633           JAXUCZ010000008.1  120086786-120086934 c
            1634-1742           JAXUCZ010000008.1  120081543-120081651 c
            1743-1806           JAXUCZ010000008.1  120079794-120079857 c
            1807-1971           JAXUCZ010000008.1  120077595-120077759 c
            1972-2064           JAXUCZ010000008.1  120077010-120077102 c
            2065-2178           JAXUCZ010000008.1  120076517-120076630 c
            2179-2589           JAXUCZ010000008.1  120074871-120075281 c
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q32"
     gene            1..2589
                     /gene="Mlh1"
                     /note="mutL homolog 1"
                     /db_xref="GeneID:81685"
                     /db_xref="RGD:620937"
     exon            1..185
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..2346
                     /gene="Mlh1"
                     /note="mismatch repair protein 1; mutL protein homolog 1;
                     mutL homolog 1, colon cancer, nonpolyposis type 2"
                     /codon_start=1
                     /product="DNA mismatch repair protein Mlh1"
                     /protein_id="NP_112315.2"
                     /db_xref="GeneID:81685"
                     /db_xref="RGD:620937"
                     /translation="MAFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKS
                     TNIQVIVKEGGLKLIQIQDNGTGIRKEDLDIVCERFTTSKLQTFEDLASISTYGFRGE
                     ALASISHVAHVTITTKTADGKCAYRASYSDGKLQAPPKPCAGNQGTLITVEDLFYNII
                     TRRKALKNPSEEYGKILEVVGRYSIHNSGISFSVKKQGETVSDVRTLPNATTVDNIRS
                     IFGNAVSRELIEVGCEDKTLAFKMNGYISNANYSVKKCIFLLFINHRLVESAALKKAI
                     EAVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILERVQQHIESKL
                     LGSNSSRMYFTQTLLPGLAGPSGEAVKSTTGIASSSTSGSGDKVHAYQMVRTDSRDQK
                     LDAFMQPVSRRLPSQPQDPVPGNRTEGSPEKAMQKDQEMSELPAAMEAAADSASLERE
                     SVIGASEVAPPAAPPSSPGSSRKRHREDSDEEMMENDSRKEMTAACYPRRRIINLTSV
                     LSLQEEINDRGHETLREMLRNHTFVGCVNPQWALAQHQTKLYLLNTTKLSEELFYQIL
                     IYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEML
                     ADYFSVEIDEEGNLIGLPLLIDSYVPPLEGLPIFILRLATEVNWDEEKECFESLSKEC
                     AVFYSIRKQYILEESALSGQQSDMPGSPSKPWKWTVEHIIYKAFRSHLLPPKHFTEDG
                     NVLQLANLPDLYKVFERC"
     exon            186..276
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            277..375
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            376..449
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            450..522
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            523..614
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            615..657
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            658..746
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            747..859
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            860..953
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1107
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1484
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            1485..1633
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1742
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1806
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1971
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            1972..2064
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            2065..2178
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
     exon            2179..2589
                     /gene="Mlh1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccaaggtgaa ggaagaactt gcgcttgagg agccgaagtg attggctgat ggcaaaatcg
       61 ggcgccaata tggcgtttgt agcaggagtt attcggcgcc tggacgagac ggtagtgaat
      121 cgcatagcgg cgggagaagt tatccagcgg ccggccaatg ctatcaaaga gatgattgaa
      181 aactgtttag atgcaaaatc tacaaatatt caagtgattg ttaaagaagg aggcctgaag
      241 ctaattcaga tccaagacaa tggcactgga atcaggaagg aagatttgga tattgtgtgt
      301 gagaggttca ctacaagtaa actgcagacc tttgaggatt tagctagtat ttccacctat
      361 ggctttcgtg gtgaggcttt ggcaagcata agccatgtgg cccatgtcac tattacaacc
      421 aaaacagctg atgggaaatg tgcatacaga gcaagttact cagatggaaa gctacaagcc
      481 cctcctaaac catgtgcagg caaccagggt accctgatca cggtggaaga ccttttttac
      541 aacataatca caaggaggaa agctttaaaa aatcccagtg aagagtacgg gaagattttg
      601 gaagttgttg gcaggtactc aatacacaat tcaggaatta gtttctcagt taaaaaacaa
      661 ggcgagacag tctctgatgt cagaacacta cccaatgcca cgaccgtaga caacattcgc
      721 tccatctttg gaaatgctgt tagtcgagaa ctgatcgaag ttgggtgtga ggataaaacc
      781 ctagctttca aaatgaatgg ctatatctcg aatgcaaact actcagtgaa gaagtgcatt
      841 ttcctactct tcatcaacca ccggctggtg gaatcggctg ccctgaagaa agccatagaa
      901 gctgtatatg cagcatattt acccaaaaac acgcacccat tcctgtacct cagtttggaa
      961 atcagtcctc agaacgtgga tgtcaatgtg caccccacca agcatgaagt tcactttctc
     1021 catgaggaga gtattctgga gcgtgtgcag cagcacatcg agagcaagct attgggctcc
     1081 aattcatcca ggatgtattt tacccagacc ttgcttccag gacttgctgg gccctctggg
     1141 gaagccgtta aatccacgac aggcatagct tcctcatcta ctagtggaag tggcgacaag
     1201 gttcacgctt accagatggt ccgtacagac tctcgggacc agaagctcga tgcctttatg
     1261 cagcctgtaa gcaggcgtct gcccagccag ccccaggacc ctgtccctgg gaacaggaca
     1321 gagggctctc ctgaaaaagc catgcagaag gatcaggaga tgtctgagct cccggccgcc
     1381 atggaagcag ctgctgacag tgcgagtttg gagagagaat cagtgattgg ggcttcggaa
     1441 gtggcacccc cagcggcacc cccttccagt ccaggcagct ccagaaagag acatcgggag
     1501 gactccgatg aggaaatgat ggaaaatgat tcccggaagg aaatgacagc tgcttgctac
     1561 ccccggagga ggatcattaa cctcaccagt gttttgagtc tccaggaaga aattaacgac
     1621 cggggtcatg agactctccg ggagatgctc cgtaaccata cctttgtggg ctgtgtgaat
     1681 cctcagtggg ccttggcaca gcaccagacc aagctctacc tcctcaacac taccaagctc
     1741 agtgaagaac tcttctacca gatactcatt tacgattttg ccaacttcgg tgttctcagg
     1801 ttatcggaac cagcaccact ctttgacctc gccatgctgg ccttagacag tcctgaaagt
     1861 ggctggacag aggaagatgg cccaaaggaa ggacttgcag aatacattgt tgagtttttg
     1921 aagaagaaag ccgagatgct tgcagactat ttctctgtgg aaattgatga ggaagggaac
     1981 ctgattggat tacctctcct gattgatagc tatgtgccgc ctttggaggg actgcctatc
     2041 ttcattcttc gactggccac tgaggtgaac tgggatgaag aaaaggagtg tttcgaaagt
     2101 ctcagtaaag aatgtgctgt gttttactcc attcggaagc aatacatact ggaggagtca
     2161 gccctctcag gccagcagag tgacatgcct ggctcccctt caaagccctg gaagtggact
     2221 gtggaacaca ttatctataa agccttccgc tcacaccttc tgcctccgaa gcatttcaca
     2281 gaagatggca atgtcctgca gctcgccaac ctgccagatc tgtacaaagt ctttgagcgg
     2341 tgctaaatgc aatcatagcc accatagaga gactgcatgg ccatccaagg tgaagcgaag
     2401 cgtatggcac tgatctggaa gccacagaat aggacacttg gtttcttagg ctccagggtt
     2461 tccagtgctc tctgttcttg ctctgtatcc cagtattggt gctgcaattt aatgcatttc
     2521 acctgtggat tagctgcaaa taaactcaca tgtgttgatg tgatttttat ataaccgcac
     2581 actggaaaa
//
