LOCUS       NM_001108105            2777 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus modulator of VRAC current 1 (Mlc1), mRNA.
ACCESSION   NM_001108105 XM_001054359 XM_235558
VERSION     NM_001108105.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Xu,H., Isenmann,S., Lopez-Hernandez,T., Estevez,R., Lukacs,G.L. and
            Apaja,P.M.
  TITLE     Control of membrane protein homeostasis by a chaperone-like glial
            cell adhesion molecule at multiple subcellular locations
  JOURNAL   Sci Rep 11 (1), 18435 (2021)
   PUBMED   34531445
  REMARK    GeneRIF: Control of membrane protein homeostasis by a
            chaperone-like glial cell adhesion molecule at multiple subcellular
            locations.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Elorza-Vidal,X., Sirisi,S., Gaitan-Penas,H., Perez-Rius,C.,
            Alonso-Gardon,M., Armand-Ugon,M., Lanciotti,A., Brignone,M.S.,
            Prat,E., Nunes,V., Ambrosini,E., Gasull,X. and Estevez,R.
  TITLE     GlialCAM/MLC1 modulates LRRC8/VRAC currents in an indirect manner:
            Implications for megalencephalic leukoencephalopathy
  JOURNAL   Neurobiol Dis 119, 88-99 (2018)
   PUBMED   30076890
  REMARK    GeneRIF: In astrocytes with reduced or increased levels of MLC1 we
            observe changes in the phosphorylation state of the VRAC subunit
            LRRC8C.
REFERENCE   3  (bases 1 to 2777)
  AUTHORS   Lanciotti,A., Brignone,M.S., Visentin,S., De Nuccio,C.,
            Catacuzzeno,L., Mallozzi,C., Petrini,S., Caramia,M., Veroni,C.,
            Minnone,G., Bernardo,A., Franciolini,F., Pessia,M., Bertini,E.,
            Petrucci,T.C. and Ambrosini,E.
  TITLE     Megalencephalic leukoencephalopathy with subcortical cysts
            protein-1 regulates epidermal growth factor receptor signaling in
            astrocytes
  JOURNAL   Hum Mol Genet 25 (8), 1543-1558 (2016)
   PUBMED   26908604
  REMARK    GeneRIF: Study discloses an important role for MLC1 in the control
            of astrocyte growth and in the regulation of pathways that trigger
            quiescent astrocytes into reactive ones in response to brain
            injury. It also shows that MLC1 pathological mutations cause loss
            of its function.
REFERENCE   4  (bases 1 to 2777)
  AUTHORS   Lanciotti,A., Brignone,M.S., Molinari,P., Visentin,S., De
            Nuccio,C., Macchia,G., Aiello,C., Bertini,E., Aloisi,F.,
            Petrucci,T.C. and Ambrosini,E.
  TITLE     Megalencephalic leukoencephalopathy with subcortical cysts protein
            1 functionally cooperates with the TRPV4 cation channel to activate
            the response of astrocytes to osmotic stress: dysregulation by
            pathological mutations
  JOURNAL   Hum Mol Genet 21 (10), 2166-2180 (2012)
   PUBMED   22328087
REFERENCE   5  (bases 1 to 2777)
  AUTHORS   Lanciotti,A., Brignone,M.S., Camerini,S., Serafini,B., Macchia,G.,
            Raggi,C., Molinari,P., Crescenzi,M., Musumeci,M., Sargiacomo,M.,
            Aloisi,F., Petrucci,T.C. and Ambrosini,E.
  TITLE     MLC1 trafficking and membrane expression in astrocytes: role of
            caveolin-1 and phosphorylation
  JOURNAL   Neurobiol Dis 37 (3), 581-595 (2010)
   PUBMED   19931615
REFERENCE   6  (bases 1 to 2777)
  AUTHORS   Ambrosini,E., Serafini,B., Lanciotti,A., Tosini,F., Scialpi,F.,
            Psaila,R., Raggi,C., Di Girolamo,F., Petrucci,T.C. and Aloisi,F.
  TITLE     Biochemical characterization of MLC1 protein in astrocytes and its
            association with the dystrophin-glycoprotein complex
  JOURNAL   Mol Cell Neurosci 37 (3), 480-493 (2008)
   PUBMED   18165104
  REMARK    GeneRIF: findings show that the membrane-associated component of
            MLC1 (60-64 kDa) localizes in astrocytic lipid rafts together with
            dystroglycan, syntrophin and caveolin-1, and co-fractionates with
            the dystrophin-glycoprotein complex in whole rat brain tissue
REFERENCE   7  (bases 1 to 2777)
  AUTHORS   Boor,I., Nagtegaal,M., Kamphorst,W., van der Valk,P., Pronk,J.C.,
            van Horssen,J., Dinopoulos,A., Bove,K.E., Pascual-Castroviejo,I.,
            Muntoni,F., Estevez,R., Scheper,G.C. and van der Knaap,M.S.
  TITLE     MLC1 is associated with the dystrophin-glycoprotein complex at
            astrocytic endfeet
  JOURNAL   Acta Neuropathol 114 (4), 403-410 (2007)
   PUBMED   17628813
REFERENCE   8  (bases 1 to 2777)
  AUTHORS   Boor,P.K., de Groot,K., Waisfisz,Q., Kamphorst,W., Oudejans,C.B.,
            Powers,J.M., Pronk,J.C., Scheper,G.C. and van der Knaap,M.S.
  TITLE     MLC1: a novel protein in distal astroglial processes
  JOURNAL   J Neuropathol Exp Neurol 64 (5), 412-419 (2005)
   PUBMED   15892299
REFERENCE   9  (bases 1 to 2777)
  AUTHORS   Teijido,O., Martinez,A., Pusch,M., Zorzano,A., Soriano,E., Del
            Rio,J.A., Palacin,M. and Estevez,R.
  TITLE     Localization and functional analyses of the MLC1 protein involved
            in megalencephalic leukoencephalopathy with subcortical cysts
  JOURNAL   Hum Mol Genet 13 (21), 2581-2594 (2004)
   PUBMED   15367490
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH474027.1.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_235558.4, XM_001054359.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN12840107,
                              SAMN12840108 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q34"
     gene            1..2777
                     /gene="Mlc1"
                     /note="modulator of VRAC current 1"
                     /db_xref="GeneID:315215"
                     /db_xref="RGD:1308552"
     exon            1..496
                     /gene="Mlc1"
                     /inference="alignment:Splign:2.1.0"
     CDS             429..1649
                     /gene="Mlc1"
                     /note="megalencephalic leukoencephalopathy with
                     subcortical cysts 1 homolog"
                     /codon_start=1
                     /product="membrane protein MLC1"
                     /protein_id="NP_001101575.1"
                     /db_xref="GeneID:315215"
                     /db_xref="RGD:1308552"
                     /translation="MNTSCWKSITQRPALPTAGPQQSAMTREGQFREELGYDRMPTLE
                     RGRQDAGRQDTGSYTPDTKPKDLQLTKRLPPCFSYKTWVFSVLMGSCLLVTSGFSLYL
                     GNVFPSEMDYLRCAAGSCIPSAIVSFAVGRRNVSAIPNFQILFVSTFAVTTTCLIWFG
                     CKLILNPSAININFNLILLLLLELLMAATVIISARSSEEPCKKKKGSISDGTNILDEV
                     TFPARVLKSYSVVEVIAGVSAVLGGVIALNVEEAVSGPHLSVTFFWILVACFPSAIAS
                     HVTAECPSKCLVEVLITISSLTSPLLFTASGYLSFSVMRIVEIFKDYPPDIKSYDVLL
                     LLLLLLLLLQGGLNTGTAIQCVSFKVSARLQAASWDSQSCPQERPAGEVVRSPLKEFD
                     KEKAWRAVVVQMAQ"
     exon            497..695
                     /gene="Mlc1"
                     /inference="alignment:Splign:2.1.0"
     exon            696..785
                     /gene="Mlc1"
                     /inference="alignment:Splign:2.1.0"
     exon            786..839
                     /gene="Mlc1"
                     /inference="alignment:Splign:2.1.0"
     exon            840..941
                     /gene="Mlc1"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1043
                     /gene="Mlc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1115
                     /gene="Mlc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1232
                     /gene="Mlc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1289
                     /gene="Mlc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1412
                     /gene="Mlc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1574
                     /gene="Mlc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1575..2777
                     /gene="Mlc1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 catggtggga ttcccggcag ttcaggggcc agccttgcca aaatcaagga cttaaaggtt
       61 tatgtggccg gacgaaggag attttcgttg tctcggaata atgggagaca aagaaagatg
      121 gcttaaaagg atgtagacct ttgagtcatt ttctgttcca ggcccaaggc tggtcaggat
      181 ccacttccag ggctggactc tatgctcagt aatgcttctt tttcaaagac atccacccag
      241 ctaggtatgc ggtgagtggg accgagggct cttcctttcc ttcttgtgtt gggcaagctc
      301 caagccttgg ttatgcaatc aaggaggaca tttctaggat ccactccctt aatctcctca
      361 ttgaaattca ggacgctgtg tgccggcccc tggctgaagt gcggtttggc tgagcttttg
      421 ctccacttat gaatacttct tgctggaagt ccatcaccca gaggcctgct ctcccaacgg
      481 ctggaccaca gcagagtgcc atgacacggg aggggcagtt cagggaggag ctgggctatg
      541 accggatgcc cacactggag aggggtcgac aggatgcagg acggcaggac acaggcagtt
      601 acacccctga cacaaagccc aaggacctgc agctgacgaa gaggttgcct ccgtgcttca
      661 gctacaagac atgggtattc tctgtgttga tggggagctg ccttctcgtg acctctgggt
      721 tttcgctcta tctggggaac gtgttcccct ccgagatgga ttatttgcgc tgtgcagcag
      781 gctcttgcat cccctcagca atcgtgagct tcgctgttgg gaggagaaat gtcagtgcga
      841 ttcccaactt tcagatattg ttcgtttcca cgttcgctgt taccactacg tgcttaatct
      901 ggtttggatg caaattgatc ctgaatcctt cagccataaa tattaatttc aatctcatcc
      961 tgctcttgct gttggagctc ctcatggctg ccacggtgat catctcggcc cggtccagtg
     1021 aggagccctg caagaagaag aagggttcca tctcggatgg cactaacatt ctggatgaag
     1081 tgacttttcc tgctcgggtc ctaaagtcct attctgtggt agaagtaatt gctggtgtct
     1141 cggctgtcct cggtggggtg atcgctctaa atgtagagga agctgtctca ggtccccatc
     1201 tctcggtgac gttcttttgg attttagtgg cttgttttcc aagtgccatc gccagccatg
     1261 tgacagcgga atgtcccagc aaatgtctgg tggaggtcct gatcaccatc agcagcctta
     1321 cgtccccact gctgttcacc gcctctggat acctgtcctt cagcgtgatg agaatcgtgg
     1381 agatttttaa agattaccca ccagacatca aatcctatga tgtgctgctg ctgctcctgc
     1441 tgctgctgct gctgcttcag gggggactca acactggcac cgccatccag tgtgtgagct
     1501 tcaaagtcag cgcccggctg caggcggcct cctgggactc ccagtcctgt ccccaggagc
     1561 ggccagcagg cgaggtggtc cgaagccccc tcaaggagtt tgacaaagaa aaagcctgga
     1621 gagctgtggt ggtacaaatg gcccagtgac tgcaggataa agaaactgag tctgggtgtc
     1681 tgcctggctc ccggcgtgtt ccactgtatg gactctaacc attgcgcaac taccgctttc
     1741 taacatactc tttccctccc gtgagggcag cccaggctgc aaggagcttt gggtttcctg
     1801 tagtagctta actttgaaat gggcaggtgt tgagtatcgg gccttgatag ttgtgtcagt
     1861 tgtgtgcatc tgaggtgacc ctgatggcct ggacaatgtt cttttcagcc ctcttcactt
     1921 cctggtttga tcctcattca caaggatctg gtgttttggc cttaggacac ttccatgcct
     1981 ttgcatgcct gccacagggt ggggatagtt tggctgtgag aatcacagat tgtttgtatg
     2041 ccccctcccc gaaccccaga ggctggctga caagagaact aaggaatcta tagtaagttg
     2101 ggtcctttgt gaaaatgggg ctcattttag gaattgcctc tgtgcttggg aggggaacct
     2161 ctgtgcttgg aaagacagtg tgcctttact gagctgggat aacaatgggc agatagggga
     2221 aagaacagag ctgccagggt cagggagaga gcgaaaatcc ccctaggctt ctcacgtgca
     2281 gatgctcacg actctgctag ggttcatgga aagcagagtc atttccaaac ggaggggtgg
     2341 gtgcaagtaa ggcacctgac agggagatgt atgagccaag agctccatct ccagtgttgg
     2401 ggattccggt cctgtcttca tctctgcgac tccagagagc tggacctgcc ccacataagt
     2461 tccatgagag gtcattctcg cctgcctatg tgcagagccc actgggcctc tggccttctg
     2521 cagctctacc acatgctacc gggtaggtag ggagccgtgg tcacactggg ctggcaagcc
     2581 atcttctggg agacttgaac tttcaggacc agaatgaggc ggcggcagga ggattgctgc
     2641 cagcttgcac tttctaattt tagagttttt tttttaaaaa gatcatataa acggggtcta
     2701 aaaaatggtc tcttgtatcc ttgtgtttga taatagttta atatgcttga gcaataaatg
     2761 ctaacacgca aatgtgg
//
