LOCUS       NM_001395047            2107 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus MIS12 kinetochore complex component (Mis12),
            transcript variant 1, mRNA.
ACCESSION   NM_001395047 XM_017597477
VERSION     NM_001395047.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Shrestha,R.L. and Draviam,V.M.
  TITLE     Lateral to end-on conversion of chromosome-microtubule attachment
            requires kinesins CENP-E and MCAK
  JOURNAL   Curr Biol 23 (16), 1514-1526 (2013)
   PUBMED   23891108
  REMARK    Erratum:[Curr Biol. 2013 Dec 2;23(23):2440-1]
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Kline,S.L., Cheeseman,I.M., Hori,T., Fukagawa,T. and Desai,A.
  TITLE     The human Mis12 complex is required for kinetochore assembly and
            proper chromosome segregation
  JOURNAL   J Cell Biol 173 (1), 9-17 (2006)
   PUBMED   16585270
REFERENCE   3  (bases 1 to 2107)
  AUTHORS   Cheeseman,I.M., Niessen,S., Anderson,S., Hyndman,F., Yates,J.R.
            3rd, Oegema,K. and Desai,A.
  TITLE     A conserved protein network controls assembly of the outer
            kinetochore and its ability to sustain tension
  JOURNAL   Genes Dev 18 (18), 2255-2268 (2004)
   PUBMED   15371340
REFERENCE   4  (bases 1 to 2107)
  AUTHORS   Goshima,G., Kiyomitsu,T., Yoda,K. and Yanagida,M.
  TITLE     Human centromere chromatin protein hMis12, essential for equal
            segregation, is independent of CENP-A loading pathway
  JOURNAL   J Cell Biol 160 (1), 25-39 (2003)
   PUBMED   12515822
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On May 28, 2021 this sequence version replaced XM_017597477.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript. All four transcripts encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643296.27562.1,
                                           SRR22582419.2618871.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               JAXUCZ010000010.1  56238317-56238461
            146-307             JAXUCZ010000010.1  56244130-56244291
            308-2107            JAXUCZ010000010.1  56244692-56246491
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q24"
     gene            1..2107
                     /gene="Mis12"
                     /note="MIS12 kinetochore complex component"
                     /db_xref="GeneID:501706"
                     /db_xref="RGD:1564837"
     exon            1..145
                     /gene="Mis12"
                     /inference="alignment:Splign:2.1.0"
     exon            146..307
                     /gene="Mis12"
                     /inference="alignment:Splign:2.1.0"
     exon            308..2107
                     /gene="Mis12"
                     /inference="alignment:Splign:2.1.0"
     CDS             341..961
                     /gene="Mis12"
                     /note="liver regeneration-related protein LRRG112; MIS12,
                     MIND kinetochore complex component, homolog"
                     /codon_start=1
                     /product="protein MIS12 homolog"
                     /protein_id="NP_001381976.1"
                     /db_xref="GeneID:501706"
                     /db_xref="RGD:1564837"
                     /translation="MSVDPMAYEAQFFGFTPQTCLLRIYIAFQDHLFEVMQAVEQVIL
                     KKLEGIPDCEISSIQTRKCTEKFLCFMKGRFDNLFGKMEQLILQSILQIPPNILLPED
                     KCQETNPFSEEKFQLLKQEIKELQEKYKVELCTEQALLAELEEQKTVKAKLRETLTFF
                     DELENIGRYQGTSNFRESLVSLVQNCRKLQDIRDNVEKESKRLETQ"
ORIGIN      
        1 gcctttgcgt tcgttcgcag aggcaggtcg cagttgattc tgaggagccg gtgtcgccgc
       61 cttattccaa gcagctcagt gtagtttgaa ggaagtggcg ggaagccggt gtgtggcgga
      121 ctacatcacc cccgggatcg cttcggatcc cagcattctg gtggtagagg cagaaggatc
      181 aaaagttcaa gaccattctc ggctacatcc caagtttgag gccagcttca gctacatgag
      241 accctgtcac aaacgaaaga gtttaaggcc aacccatagg ctgcacagca agaaattgtc
      301 ttaagaggtt ttcacagctg aaggctcaag gtaaagcagg atgtctgtgg atccgatggc
      361 ctatgaggcc cagttctttg gcttcacccc acagacttgc ttgctgagga tctacatagc
      421 atttcaagac cacctctttg aagtgatgca ggctgttgaa caggttatct taaagaagct
      481 ggagggcatt ccagactgtg agattagctc tatccagact cgtaaatgca cagagaagtt
      541 tctttgcttc atgaaaggac gttttgataa cctttttggt aaaatggagc agctgatttt
      601 acaatcgatt ttgcaaattc ctccaaacat cctgcttcct gaagataagt gtcaggagac
      661 gaatcctttc agtgaagaaa aattccagct tctcaaacag gaaatcaaag agttacagga
      721 gaaatataag gttgaattat gcactgagca ggcccttctt gcagaattag aggagcaaaa
      781 aactgttaaa gccaaactca gagagacgtt gactttcttt gatgagcttg aaaacatcgg
      841 caggtatcaa ggaactagta actttaggga gagtttggtg tctctggtcc agaactgcag
      901 aaaactccag gatattagag ataatgtaga aaaagagagc aaaaggctgg aaacacaata
      961 atttctcagt agtgaagaga gagcatgtaa atagatcgct tttctttgtt tactcttatg
     1021 tttaaggtct tttgggggcc acgttcccct cctttcctag gttacacaaa gccagtcagg
     1081 agatcggtat tgctgcaggc tccaggtcag acttctaaag cagagtttgc gttcatttgc
     1141 ttttctccct ctgaaacttg agttgaccac cctcacattt ggtaatgact tcagttcttt
     1201 tgtaactgta ctcattttac tgttagggat gttatagtat gatttcagga gggaatagaa
     1261 tcctcatgga actggatgct tctgtgtaaa cctggtctgg gtatttattg agtataacca
     1321 tgaagtacat tagagcagaa aagctaggct gaggcatagc tgagtggtag agtatgcttg
     1381 gcatgtgggt ccctcgggat ttaacatggg agagaagcaa aaagaggagc aggaagaagg
     1441 aaaagcaaac aaaggacagc aggatacagc agaggtgtgg gggtcaggga tatgggatgg
     1501 tgtatattgc agtatgagaa agggcatgtc tgataatgta agattcaagt cacacataga
     1561 aggcgttagc ctcgtgctta tgggattatc ctaggcaaag aaaacagtag aaaacccata
     1621 aggtagagca tattaaggaa ttcacacagc tggaacagat atggaagtgt aggaaaaggg
     1681 gatacacctg tcctgttcca ggctagtttt tgtaagacag acaagggctg atgagatggg
     1741 tcagcaggta aaagtacttg tagtacaagc ctgctgacct gtgtaggatt cctagaacct
     1801 acttaaagat ggaaaggagg cttgactcca cagattgtcc tctgattgct aaaggtacac
     1861 tggcatgtgc actcccatct cccacattgt gtatgcacac acagtgattt atatatctta
     1921 agagacaagc aaaacccctg tgtatgtgtg aatgtgtact attaaaactg actcctgagg
     1981 ggttggggat ttggctcagt ggtagagcgc ttgcctagca agcgcaaggc cctgggttcg
     2041 gtccccagct ccgaaaaaaa gaaagggaaa aaaaacaaaa caaaacaaaa caaaacaaaa
     2101 aaaaaaa
//
