LOCUS       NM_001105719             991 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus major intrinsic protein of lens fiber (Mip),
            mRNA.
ACCESSION   NM_001105719 XM_001070399 XM_343137
VERSION     NM_001105719.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Kumari,S.S., Gandhi,J., Mustehsan,M.H., Eren,S. and Varadaraj,K.
  TITLE     Functional characterization of an AQP0 missense mutation, R33C,
            that causes dominant congenital lens cataract, reveals impaired
            cell-to-cell adhesion
  JOURNAL   Exp Eye Res 116, 371-385 (2013)
   PUBMED   24120416
REFERENCE   2  (bases 1 to 991)
  AUTHORS   Watanabe,K., Wada,K., Ohashi,T., Okubo,S., Takekuma,K.,
            Hashizume,R., Hayashi,J., Serikawa,T., Kuramoto,T. and Kikkawa,Y.
  TITLE     A 5-bp insertion in Mip causes recessive congenital cataract in
            KFRS4/Kyo rats
  JOURNAL   PLoS One 7 (11), e50737 (2012)
   PUBMED   23226368
  REMARK    GeneRIF: the kfrs4 rat represents the first characterised rat model
            with a recessive mutation in the Mip gene
REFERENCE   3  (bases 1 to 991)
  AUTHORS   Nakazawa,Y., Oka,M., Mitsuishi,A., Bando,M. and Takehana,M.
  TITLE     Quantitative analysis of ascorbic acid permeability of aquaporin 0
            in the lens
  JOURNAL   Biochem Biophys Res Commun 415 (1), 125-130 (2011)
   PUBMED   22020074
  REMARK    GeneRIF: These findings suggest that AQP0 plays an important role
            in controlling lens ascorbic acid content.
REFERENCE   4  (bases 1 to 991)
  AUTHORS   Yakata,K., Tani,K. and Fujiyoshi,Y.
  TITLE     Water permeability and characterization of aquaporin-11
  JOURNAL   J Struct Biol 174 (2), 315-320 (2011)
   PUBMED   21251984
REFERENCE   5  (bases 1 to 991)
  AUTHORS   Grey,A.C., Li,L., Jacobs,M.D., Schey,K.L. and Donaldson,P.J.
  TITLE     Differentiation-dependent modification and subcellular distribution
            of aquaporin-0 suggests multiple functional roles in the rat lens
  JOURNAL   Differentiation 77 (1), 70-83 (2009)
   PUBMED   19281766
  REMARK    GeneRIF: These results show that changes in the subcellular
            distribution and processing of AQP0 occur at discrete stages during
            fiber cell differentiation in the eye lens and suggest that these
            stages of differentiation.
REFERENCE   6  (bases 1 to 991)
  AUTHORS   Zeidel,M.L., Ambudkar,S.V., Smith,B.L. and Agre,P.
  TITLE     Reconstitution of functional water channels in liposomes containing
            purified red cell CHIP28 protein
  JOURNAL   Biochemistry 31 (33), 7436-7440 (1992)
   PUBMED   1510932
REFERENCE   7  (bases 1 to 991)
  AUTHORS   Preston,G.M., Carroll,T.P., Guggino,W.B. and Agre,P.
  TITLE     Appearance of water channels in Xenopus oocytes expressing red cell
            CHIP28 protein
  JOURNAL   Science 256 (5055), 385-387 (1992)
   PUBMED   1373524
REFERENCE   8  (bases 1 to 991)
  AUTHORS   Kent,N.A. and Shiels,A.
  TITLE     Nucleotide and derived amino-acid sequence of the major intrinsic
            protein of rat eye-lens
  JOURNAL   Nucleic Acids Res 18 (14), 4256 (1990)
   PUBMED   2377471
REFERENCE   9  (bases 1 to 991)
  AUTHORS   Shiels,A., Kent,N.A., McHale,M. and Bangham,J.A.
  TITLE     Homology of MIP26 to Nod26
  JOURNAL   Nucleic Acids Res 16 (19), 9348 (1988)
   PUBMED   3174458
REFERENCE   10 (bases 1 to 991)
  AUTHORS   Lyon,M.F., Jarvis,S.E., Sayers,I. and Holmes,R.S.
  TITLE     Lens opacity: a new gene for congenital cataract on chromosome 10
            of the mouse
  JOURNAL   Genet Res 38 (3), 337-341 (1981)
   PUBMED   7333462
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Mar 29, 2021 this sequence version replaced NM_001105719.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X53040.1, SRR26360190.898927.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN06680424, SAMN06680427
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-404               JAXUCZ010000007.1  1231905-1232308
            405-569             JAXUCZ010000007.1  1233083-1233247
            570-650             JAXUCZ010000007.1  1233770-1233850
            651-991             JAXUCZ010000007.1  1237367-1237707
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..991
                     /gene="Mip"
                     /gene_synonym="Aqp0"
                     /note="major intrinsic protein of lens fiber"
                     /db_xref="GeneID:25480"
                     /db_xref="RGD:3090"
     exon            1..404
                     /gene="Mip"
                     /gene_synonym="Aqp0"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..836
                     /gene="Mip"
                     /gene_synonym="Aqp0"
                     /EC_number="1.11.1.6"
                     /note="major intrinsic protein of eye lens fiber; MP26;
                     MIP26; aquaporin-0"
                     /codon_start=1
                     /product="lens fiber major intrinsic protein"
                     /protein_id="NP_001099189.1"
                     /db_xref="GeneID:25480"
                     /db_xref="RGD:3090"
                     /translation="MWELRSASFWRAIFAEFFATLFYVFFGLGSSLRWAPGPLHVLQV
                     ALAFGLALATLVQTVGHISGAHVNPAVTFAFLVGSQMSLLRAFCYIAAQLLGAVAGAA
                     VLYSVTPPAVRGNLALNTLHAGVSVGQATTVEIFLTLQFVLCIFATYDERRNGRMGSV
                     ALAVGFSLTLGHLFGMYYTGAGMNPARSFAPAILTRNFSNHWVYWVGPIIGGGLGSLL
                     YDFLLFPRLKSVSERLSILKGARPSDSNGQPEGTGEPVELKTQAL"
     exon            405..569
                     /gene="Mip"
                     /gene_synonym="Aqp0"
                     /inference="alignment:Splign:2.1.0"
     exon            570..650
                     /gene="Mip"
                     /gene_synonym="Aqp0"
                     /inference="alignment:Splign:2.1.0"
     exon            651..991
                     /gene="Mip"
                     /gene_synonym="Aqp0"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agggggttat tagggaccca ggcactgtga ttgcccccac tgccatgtgg gaacttcggt
       61 ctgcctcctt ttggagggcc atatttgctg agttctttgc taccctcttc tatgtcttct
      121 ttgggctggg atcctcactg cgttgggccc ctggacccct acatgtcctg caagtggctt
      181 tggcctttgg cctggccctg gccacactgg tgcagactgt gggtcacatc agtggagccc
      241 atgtcaaccc tgcggtcact tttgccttcc ttgtgggctc ccagatgtcc ttgctccgtg
      301 ctttctgcta tatagcagcc cagcttctgg gagctgtggc tggagcggct gtgctgtaca
      361 gtgtcacccc accagctgtc cgaggaaacc tagcactcaa cacgctgcat gctggggtaa
      421 gcgtgggcca ggccaccact gtggagatct tcctgacgct ccagttcgtg ctctgcatct
      481 ttgctacgta tgacgagagg cggaatggcc gcatgggctc tgtggccctg gctgttggct
      541 tctccctcac cctggggcac ctctttggga tgtattacac tggcgcgggg atgaatcccg
      601 cccgctcctt tgctcctgct atcctcacca ggaacttcag caaccactgg gtgtactggg
      661 tgggcccaat catcggcggg ggcctgggca gcctgctcta cgactttctc ctcttcccac
      721 ggctcaagag tgtttctgag agactgtcta tcctcaaggg agccagaccc agtgactcca
      781 atggacagcc agagggtaca ggggaacctg tggaactgaa gactcaggcc ctgtaaaagc
      841 tgcagaacgc ccgctgggtt tcagggaggg ccaagccagc ccttggatga agaaagagac
      901 tgtggggagg ggcatgtact tctttatttt ttaacttacg tttttcattt tttccttttg
      961 ctgtgtgaaa tctttcaagt tgcattcacg a
//
