LOCUS       NM_001191889            2631 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus midline 2 (Mid2), mRNA.
ACCESSION   NM_001191889 XM_343825
VERSION     NM_001191889.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Kimura,T., Jain,A., Choi,S.W., Mandell,M.A., Schroder,K.,
            Johansen,T. and Deretic,V.
  TITLE     TRIM-mediated precision autophagy targets cytoplasmic regulators of
            innate immunity
  JOURNAL   J Cell Biol 210 (6), 973-989 (2015)
   PUBMED   26347139
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Uchil,P.D., Hinz,A., Siegel,S., Coenen-Stass,A., Pertel,T.,
            Luban,J. and Mothes,W.
  TITLE     TRIM protein-mediated regulation of inflammatory and innate immune
            signaling and its association with antiretroviral activity
  JOURNAL   J Virol 87 (1), 257-272 (2013)
   PUBMED   23077300
REFERENCE   3  (bases 1 to 2631)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   4  (bases 1 to 2631)
  AUTHORS   Uchil,P.D., Quinlan,B.D., Chan,W.T., Luna,J.M. and Mothes,W.
  TITLE     TRIM E3 ligases interfere with early and late stages of the
            retroviral life cycle
  JOURNAL   PLoS Pathog 4 (2), e16 (2008)
   PUBMED   18248090
REFERENCE   5  (bases 1 to 2631)
  AUTHORS   Short,K.M., Hopwood,B., Yi,Z. and Cox,T.C.
  TITLE     MID1 and MID2 homo- and heterodimerise to tether the
            rapamycin-sensitive PP2A regulatory subunit, alpha 4, to
            microtubules: implications for the clinical variability of X-linked
            Opitz GBBB syndrome and other developmental disorders
  JOURNAL   BMC Cell Biol 3, 1 (2002)
   PUBMED   11806752
REFERENCE   6  (bases 1 to 2631)
  AUTHORS   Buchner,G., Montini,E., Andolfi,G., Quaderi,N., Cainarca,S.,
            Messali,S., Bassi,M.T., Ballabio,A., Meroni,G. and Franco,B.
  TITLE     MID2, a homologue of the Opitz syndrome gene MID1: similarities in
            subcellular localization and differences in expression during
            development
  JOURNAL   Hum Mol Genet 8 (8), 1397-1407 (1999)
   PUBMED   10400986
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000021.1.
            
            [WARNING] On Oct 24, 2024 this sequence was replaced by
            NM_001191889.2.
            
            On Jul 17, 2010 this sequence version replaced XM_343825.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360194.1694828.1,
                                           SRR22582419.683634.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760386, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-168               JAXUCZ010000021.1  109143820-109143987
            169-884             JAXUCZ010000021.1  109157236-109157951
            885-980             JAXUCZ010000021.1  109173763-109173858
            981-1088            JAXUCZ010000021.1  109213866-109213973
            1089-1237           JAXUCZ010000021.1  109215819-109215967
            1238-1365           JAXUCZ010000021.1  109227017-109227144
            1366-1599           JAXUCZ010000021.1  109228287-109228520
            1600-1761           JAXUCZ010000021.1  109238809-109238970
            1762-1969           JAXUCZ010000021.1  109240546-109240753
            1970-2631           JAXUCZ010000021.1  109241312-109241973
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq33"
     gene            1..2631
                     /gene="Mid2"
                     /gene_synonym="RGD1565674"
                     /note="midline 2"
                     /db_xref="GeneID:363502"
                     /db_xref="RGD:1565674"
     exon            1..168
                     /gene="Mid2"
                     /gene_synonym="RGD1565674"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..2372
                     /gene="Mid2"
                     /gene_synonym="RGD1565674"
                     /EC_number="2.3.2.27"
                     /codon_start=1
                     /product="probable E3 ubiquitin-protein ligase MID2"
                     /protein_id="NP_001178818.1"
                     /db_xref="GeneID:363502"
                     /db_xref="RGD:1565674"
                     /translation="MGESPASAVLNASAGLFSLKMETLESELTCPICLELFEDPLLLP
                     CAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNI
                     IDRFQKASVSGPNSPSESRRERTYRPGSAMSSERIACQFCEQDPPRDAVKTCITCEVS
                     YCDRCLRATHPNKKPFTSHRLVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALC
                     KLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTA
                     MHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLIN
                     QAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKL
                     LEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISK
                     SWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTR
                     YIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKS
                     HTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASS
                     WVFSRCNSHFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVT
                     FILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH"
     exon            169..884
                     /gene="Mid2"
                     /gene_synonym="RGD1565674"
                     /inference="alignment:Splign:2.1.0"
     exon            885..980
                     /gene="Mid2"
                     /gene_synonym="RGD1565674"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1088
                     /gene="Mid2"
                     /gene_synonym="RGD1565674"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1237
                     /gene="Mid2"
                     /gene_synonym="RGD1565674"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1365
                     /gene="Mid2"
                     /gene_synonym="RGD1565674"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1599
                     /gene="Mid2"
                     /gene_synonym="RGD1565674"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..1761
                     /gene="Mid2"
                     /gene_synonym="RGD1565674"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1969
                     /gene="Mid2"
                     /gene_synonym="RGD1565674"
                     /inference="alignment:Splign:2.1.0"
     exon            1970..2631
                     /gene="Mid2"
                     /gene_synonym="RGD1565674"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagcggagaa ggcgggcggc ggcctagagt ggtggtggcg gcggcggcgg cagcgacagc
       61 ggcccgggag cttgcgcggg agcccgagcc agcttgctgt ggaggccagc gagcgcacgg
      121 cgcttcccgg cgcgcgtagg agatggctga cacccgggaa caatatgggt gagagcccag
      181 cctctgcggt tcttaatgcc tcagcaggat tattttcact aaagatggaa acactggagt
      241 ctgaattgac ctgtccaatc tgcctcgagt tgtttgaaga cccccttctg ctcccttgtg
      301 ctcacagcct atgttttagc tgtgctcacc gcatcctggt ctcaagctgc agctccggtg
      361 aatccattga acccattact gcttttcagt gtcctacatg caggtatgtt atctcgctga
      421 atcaccgggg cctggatggc ctcaagagga atgtgaccct gcagaacatt attgatcgct
      481 tccagaaggc ttcagtcagt gggcccaatt ctccaagtga gagccgtcgg gagaggactt
      541 acaggcctgg ttccgccatg tcgagtgagc gaattgcatg tcagttctgt gagcaggacc
      601 ctccgagaga tgctgtaaag acatgcatca cctgtgaggt ctcctactgt gaccggtgcc
      661 ttcgggccac acaccccaac aagaaacctt tcacgagcca tcgcctggtg gaaccagttt
      721 cagacacaca tcttcgaggg attacctgtc tggaccatga gaatgagaag gtgaacatgt
      781 actgtgtatc tgatgaccaa ttgatctgtg ccttatgcaa actggtgggt cgtcaccgag
      841 accatcaggt cgcttctctg aatgatcgat ttgagaaact aaagcaaact ctcgagatga
      901 acctcaccaa cctggttaag cgcaacagcg aactagaaaa tcaaatggcc aaactaatac
      961 agatctgcca gcaagttgag gtgaatactg ctatgcacga ggcaaaactt atggaagagt
     1021 gtgacgagct ggtagagatc atccagcaga ggaaacaaat gattgccgtc aaaatcaaag
     1081 agacaaaggt tatgaaactg agaaagttgg cccagcaggt tgctaattgc cgccagtgtc
     1141 ttgaacggtc aacagtcctc atcaaccagg ctgagcatat cctaaaggaa aacgaccaag
     1201 ctcgctttct gcagtctgca aaaaatattg ccgagagggt cgctatggca actgcatctt
     1261 cgcaagttct gattccagac atcaatttta atgatgcctt tgaaaacttt gctttagatt
     1321 tttctagaga aaagaaacta ttggaggggc tagactattt aacagctcca aacccaccat
     1381 ctatccgaga agaactctgt actgcttccc atgacaccat tacggtccac tggatctcag
     1441 acgacgagtt cagcatcagc tcctatgagc ttcagtacac catattcact ggccaggcta
     1501 acttcatcag taagtcatgg tgtagttggg gcctgtggcc agagataagg aaatgtaagg
     1561 aagcagtaag ctgctcaaga ctggccggtg cgccacgagg cctgtacaat tcagtggaca
     1621 gctggatgat tgtgcccaac attaagcaga accattacac agtccatgga ctccagagtg
     1681 ggacacgcta catctttatt gtgaaagcca taaatcaagc aggcagccgg aacagtgaac
     1741 ccaccaggct aaaaacaaac agccaaccat ttaaactaga tcccaaaatg actcacaaga
     1801 agttgaagat ctccaatgat ggattgcaga tggagaagga cgagagctct ctgaagaaga
     1861 gccacactcc ggagagattt agtggcacgg ggtgttatgg ggcagcagga aatatattca
     1921 ttgacagtgg ctgccactac tgggaggtgg tcatgggttc ttcaacatgg tatgcaatcg
     1981 gtattgccta caaatcagct ccaaagaacg agtggattgg caagaatgcc tcttcctggg
     2041 tcttctctcg atgcaacagt cacttcgtgg tgcgacataa caacaaggaa atgctggtgg
     2101 atgtgccccc acagttgaag cgtctaggcg tcctcttgga ttatgacaac aacatgctgt
     2161 ctttctatga cccagctaac tctctccatc ttcatacttt tgatgttacc ttcattcttc
     2221 cagtttgtcc aacattcaca atctggaaca aatccctaat gatcctgtct ggcttgcctg
     2281 ccccagattt tattgattat cctgagcggc aggaatgcaa ctgcaggcct caagaatccc
     2341 cgtatgtgtc ggggatgaaa gcttgtcatt aagtgtcaag agagcatgta agtcgctggc
     2401 tctccggttc agcacttctg cccttctcaa gcctccatta gtgctccata tatgagatac
     2461 aaactaaagt tcgttgggag cacccaaaat aactagatat tgtagactta atttttgtgc
     2521 attgaaactt atgtttgaat tcatgtattg agttactcag aacaaattaa tctgaatggt
     2581 ctgggttcat gccaccaggg aagaatttta gagaatatct ctggcgtcat t
//
