LOCUS       XM_017602142            6076 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus midline 1 (Mid1), transcript variant
            X2, mRNA.
ACCESSION   XM_017602142
VERSION     XM_017602142.2
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051356.1) annotated using gene prediction method: Gnomon,
            supported by mRNA and EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_017602142.3.
            
            On Jan 21, 2021 this sequence version replaced XM_017602142.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..6076
                     /gene="Mid1"
                     /gene_synonym="Fxy; Midline1"
                     /note="midline 1; Derived by automated computational
                     analysis using gene prediction method: Gnomon. Supporting
                     evidence includes similarity to: 3 mRNAs, 18 ESTs, 13
                     Proteins, and 100% coverage of the annotated genomic
                     feature by RNAseq alignments, including 37 samples with
                     support for all annotated introns"
                     /db_xref="GeneID:54252"
                     /db_xref="RGD:2640"
     CDS             428..2431
                     /gene="Mid1"
                     /gene_synonym="Fxy; Midline1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase Midline-1 isoform
                     X1"
                     /protein_id="XP_017457631.1"
                     /db_xref="GeneID:54252"
                     /db_xref="RGD:2640"
                     /translation="METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCAT
                     NEPVESINAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRR
                     ERAFDANTMSSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHR
                     LIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDK
                     LKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQER
                     RQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKN
                     ITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELC
                     TASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTV
                     HGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVER
                     DESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHE
                     WIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHPRRVGILLDYDNGSIAFYDALNSI
                     HLYTFDVALAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP"
ORIGIN      
        1 tacaggccaa ctgacagagc ccttctggca tgtatagagt taactaggtt acgggggttc
       61 ttagttaact tctttcacca ggaatgcaat tcatgtttac ctatataagg aaacctgtta
      121 ggctgacctg agtttggctt tttttttttc tctgactgag aggcatgtaa gcacacaccc
      181 tggagagggg gagcgattcc cagctggaca gagcaagctt cctgaacgcc ccagccattc
      241 agctgatccg aggcgaagag cagagtccgt acttgccctg gagcagcctc cttggaggac
      301 ccacaggttt gccttttcca gctcccagtt gagaaacaaa cctacgatcc tgggaaaaaa
      361 tggggaagcc gatagctgat cagcttcctt ggattttgct gatgaccccg gagagttttg
      421 cctgaagatg gaaacactgg agtcggagct gacctgtcct atttgtctgg agctttttga
      481 ggaccctctc ctgcttccct gtgcacatag cctctgtttc aactgtgccc accgcatcct
      541 ggtctctcac tgtgccacca acgagcctgt ggagtccatc aacgccttcc agtgccccac
      601 ctgccggcat gtcatcacgc tcagccagcg aggtctagac gggctcaagc gcaacgtcac
      661 cttacaaaac atcattgaca ggtttcagaa agcatccgtg agtgggccca attctcccag
      721 tgagacccgc agggagcggg cctttgacgc caacaccatg tcttctgccg agaaagttct
      781 ctgccagttc tgtgaccagg atcctgccca ggatgctgtg aagacctgtg ttacttgcga
      841 agtgtcctac tgtgatgagt gcctgaaagc cactcatccg aataagaagc cctttacagg
      901 ccatcgtctg attgagccaa tcccggactc gcatatccgg gggctaatgt gcttggagca
      961 tgaggatgag aaggtgaata tgtactgtgt gaccgatgac cagttaatct gtgccttgtg
     1021 taaactggtt gggcggcacc gcgatcatca ggtggcagct ttgagtgagc gctatgacaa
     1081 attgaagcaa aatttggaga gtaatctcac caatcttatt aagagaaaca cagaactgga
     1141 gacccttttg gctaaactca tccaaacttg tcaacatgtt gaggtcaatg catcccgtca
     1201 agaagccaag ttgacagagg aatgtgatct tctcattgaa attattcagg aaagaagaca
     1261 aattattgga acaaagatta aagaaggcaa ggtgatgagg ctccgcaaat tagctcagca
     1321 gattgcaaac tgtaaacaat gcattgaaag gtctgcatca ctcatttccc aagcagaaca
     1381 ctcactgaag gagaatgacc atgcccgttt tctacagaca gcaaagaaca tcactgagag
     1441 agtctccatg gcaactgcat cctcccaggt cctaattcct gaaatcaacc tcaatgacac
     1501 atttgacacc tttgccttag atttttcccg ggagaagaaa ctgctagaat gtttggatta
     1561 cctaacagct cccaaccctc ccacaattag agaagagctc tgcacggctt cctatgacac
     1621 catcactgtc cactggactt cggatgatga gttcagtgtg gtctcctatg aactccagta
     1681 caccatattc actggacaag ccaatgtcgt tagtctttgt aactcggcag atagctggat
     1741 gattgtgccc aacatcaagc agaaccacta tactgtgcat ggcctacaaa gtggcaccaa
     1801 gtatatcttc atggtcaagg ccatcaacca ggcaggcagc cgcagcagtg agcctgggaa
     1861 gctgaagaca aacagtcagc catttaaact ggatcccaaa tctgctcatc gaaaactcaa
     1921 ggtgtcccat gacaacctga ctgttgaacg tgatgagtca tcatccaaaa agagtcacac
     1981 acctgaacgc ttcactagcc aagggagcta tggcgtggct ggaaatgtgt ttattgacag
     2041 tggccgtcat tactgggaag tggtcataag cggaagcaca tggtatgcca ttggccttgc
     2101 gtacaaatca gctccaaaac atgaatggat tgggaagaat tctgcttcct gggccctttg
     2161 ccgctgcaac aataactggg tggtgagaca taacagcaag gaaataccca ttgaaccagc
     2221 cccccacccc cggcgtgtag gcatcctgct ggactatgat aatggctcaa tcgcttttta
     2281 tgatgctttg aactccatcc acctctatac cttcgatgtg gcgcttgcgc agcctgtgtg
     2341 ccccaccttt actgtgtgga acaagtgttt gacgattata actggtcttc ccatcccaga
     2401 ccatttggac tgtacagaac agctaccttg agcatctggc cccagagcag ccccaaggaa
     2461 ttacagaggt tgaggatgtt gtttatggga ttgagaaact aaggcttcaa gagtgtgatg
     2521 aaatattccc caacttgtcc agaaaccagc tcagataggg ctccacatgg aagatttccc
     2581 tccttttcct gtgtttcgta ttaagtacag gctttaataa tttctttact tttttgtatt
     2641 tagaggaaaa tctatagatt atttataaaa gaagcataat ctggattaca gctgttagga
     2701 atcacttggt tttgtccaat aagaggcccg taagtttacc agtcctttac cagtttcatt
     2761 tcatcagtct atgagcctgt aaaaaatttt ttgtagattt gtgtggtttt ttttcacttt
     2821 cttacattct actccatact gaggccatgc taggtactgt cacttagaac aactgaatat
     2881 atggctaaaa taaaagtaat tttgatcctt gtaaggaagt acaggttcct ttactctttt
     2941 agtcaagata ttttaactgt gatatcagat cacttgtact ggatcaaata gaatgagaaa
     3001 aaatcgagaa ggaaagaatt aactttggac ctcaagctta cccagactga tgggatgatt
     3061 tggaaattct taaaagtttt gttatatatt atcacccact tctccagggc tgaagctttt
     3121 gggctaatat cttgaatgag attacaatta aatgttgtca tttgatttct acaagtgatt
     3181 taagaattca tgctgatttg ggttttatca aaagcagaat tctgttttat ggtataaaac
     3241 tgttttactt tacctcttac cagtcatttt tgggcctatg aaattttttc actaaattta
     3301 ataggtgccc tattattcca ttccacaaat caaattttca tttctttctg taactataga
     3361 tattaacatg gccttttaca aatagcaatc ccactttcaa ctgattttgt tttctgggac
     3421 atcagcacat gcccttgaac actgtccaaa tataagtcac caaaaacagt atttatgagt
     3481 aatgataaga catattaaac aaagtactag tcactagtgt acatgccttt aaagttacag
     3541 tgttttagta agcacatttt gaagatgtag acatccccat atatcctagt atagtttatc
     3601 atagagttgt aagaaaaaaa tctaatttgc tgttcctaca gaagaatctg ccaatagaac
     3661 ctactaataa aattgcagat agaattcttt tcatactgac ctgatatact gtagtggtct
     3721 ctaaattaag actttctcag gtgatcttgg catgggacat tgacatgggt tcaccttgat
     3781 aaaagacatt tacctgtata agtggtttca actgctaaaa tatttatctt ggccaccaca
     3841 tcagccagcc attgactgaa aagactgatg gattgaggca ttggttttgg tgattttgtt
     3901 tttaatcaaa tcaaccattt cccagaaata actcaataca ctcccccttt cagcgtcaca
     3961 tggtcacaat taatgtgcat gccaatcttg acttcatgga agacaaagcc aatttcctgt
     4021 gtctttctgt cccttccttg gcagattttg tccttttttt taatggctag atttttatat
     4081 attgactata ttatttatac ccagatgaat attttgatag caacttaaga caatttcaca
     4141 tcttaaaaat ggacacctca gtggaaaaac tttcattttt ctctaacaat ttattaggac
     4201 tttttaacag tataaaaaaa tgtgtatttg ccttccctga ataaaccgaa aaggttgtct
     4261 cagtcatttt tatgtacatt ttgggtaact ggataataga tattttaatg cactttgtac
     4321 tcttacaggt tctaaacaaa agggtctaaa actcaaattc tgagttttta aaaactcatg
     4381 gtctccaggt aacaataaat gctacagttt gccttatgat gttcacataa agcatttatg
     4441 aacaggacaa tatttccatg aatatgtttt tcagtataaa gatgttacta ctcagatttt
     4501 cactgcaagc tttttagggt gtgttttggc tatttttgta aggacaagac agattatgtt
     4561 ataattttgt tgtgcaagtc ttactcttgg ctaacttaaa aacattgcgg atgtagcagt
     4621 tactatttcc aggttgtcat atttacagga aaatgtgtat atggtgaaag accacagtgt
     4681 ttaattgctg taatgatgta gaaaagattt ccatgtggat tttttaagtt taaaaataca
     4741 tgatgtaaac tttgctgcag tataactctg cattttcttt aatggactgg ggccactcaa
     4801 aggaagcatt tatatagagg ggcaaaagag atggcagagt caattgaatt agtacaacaa
     4861 aaatgatgag atgccaatga aggctgtaaa gccttggcac agtgtgtatg tgaataactc
     4921 agttgccaag tgccctgagc acaaaaaggg cctaagcttt gtatcatgtt atcttttcct
     4981 tgttatgaat cttagtggtt tttttgaaca tggaggtcat cattttcact tttgcactgg
     5041 gccttgcata ttatgtagcc attccttgga acaggactcc aaatcatggt actgctcttc
     5101 ctaatggata aagagaaaca tgttgcttat tgaaacttgc tttagattca tactcaaatc
     5161 tgaatttaga taatagataa gatgagagtg gatgtagagt gaagattttc tctccagctt
     5221 taaaagaaag tgacttcaga aagtgactgt gcagatggtg gtttctgtgg agtgaaaagc
     5281 aacctgaaaa tggacagtct tttattatgg tactgatata tggaccttga acttcctgag
     5341 gaatgagcca gtgccctcta ctcaagatgg atttgtaact cagctccagg tttgcatgta
     5401 aatggctgca gagagacatg tgactgcctg ttcagtgcat ctggtgctgg gaagctgaca
     5461 tctggtctct cttggtaaaa catcaatggc atgcagagac aatgacacta gcaagagaaa
     5521 tgttttgaaa atagtgcttc tcttcaagtt cttgattttg ctacccaaga agacatttgg
     5581 ccttatttgg agtcaatttt ggtagttgca agttggagac atgttgctcc tggcatctgg
     5641 ttggtgcaag tcagaaatgg catttaacat cccacagtgc acagtgaagc ttctcatcag
     5701 aaaatgatat atagctcaaa atgtcagctg tttcctagtt tagctactct tgaatgttag
     5761 atttttttca gggataaaca aacaaaaagg cacagaagtc aataacttca cagaagtggc
     5821 actatcatcc ttaaaattat ttaaaaatac agaaaacctg atttctaaag caatccaaga
     5881 gggtgagaat gcacattatt gaaataagtg ttgtgatttt attatcttgc tgaaatgatg
     5941 tttttttctg tcttgttgga ccagtatact ttcaatccct ggtacttgag tcaactgcca
     6001 agcccctcag tccagaatcg attcttgctt actgttgctt cctggttcaa aataaaatcc
     6061 ttttgtggca ccagaa
//
