LOCUS       NM_134396               2348 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus mitochondrial calcium uptake 2 (Micu2), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_134396
VERSION     NM_134396.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Vishnu,N., Hamilton,A., Bagge,A., Wernersson,A., Cowan,E.,
            Barnard,H., Sancak,Y., Kamer,K.J., Spegel,P., Fex,M., Tengholm,A.,
            Mootha,V.K., Nicholls,D.G. and Mulder,H.
  TITLE     Mitochondrial clearance of calcium facilitated by MICU2 controls
            insulin secretion
  JOURNAL   Mol Metab 51, 101239 (2021)
   PUBMED   33932586
  REMARK    GeneRIF: Mitochondrial clearance of calcium facilitated by MICU2
            controls insulin secretion.
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Matesanz-Isabel,J., Arias-del-Val,J., Alvarez-Illera,P.,
            Fonteriz,R.I., Montero,M. and Alvarez,J.
  TITLE     Functional roles of MICU1 and MICU2 in mitochondrial Ca(2+) uptake
  JOURNAL   Biochim Biophys Acta 1858 (6), 1110-1117 (2016)
   PUBMED   26903221
REFERENCE   3  (bases 1 to 2348)
  AUTHORS   Tsai,M.F., Phillips,C.B., Ranaghan,M., Tsai,C.W., Wu,Y.,
            Willliams,C. and Miller,C.
  TITLE     Dual functions of a small regulatory subunit in the mitochondrial
            calcium uniporter complex
  JOURNAL   Elife 5, e15545 (2016)
   PUBMED   27099988
  REMARK    Erratum:[Elife. 2017 Mar 02;6:e26228. doi: 10.7554/eLife.26228..
            PMID: 28253172]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2348)
  AUTHORS   Petrungaro,C., Zimmermann,K.M., Kuttner,V., Fischer,M., Dengjel,J.,
            Bogeski,I. and Riemer,J.
  TITLE     The Ca(2+)-Dependent Release of the Mia40-Induced MICU1-MICU2 Dimer
            from MCU Regulates Mitochondrial Ca(2+) Uptake
  JOURNAL   Cell Metab 22 (4), 721-733 (2015)
   PUBMED   26387864
REFERENCE   5  (bases 1 to 2348)
  AUTHORS   Patron,M., Checchetto,V., Raffaello,A., Teardo,E., Vecellio
            Reane,D., Mantoan,M., Granatiero,V., Szabo,I., De Stefani,D. and
            Rizzuto,R.
  TITLE     MICU1 and MICU2 finely tune the mitochondrial Ca2+ uniporter by
            exerting opposite effects on MCU activity
  JOURNAL   Mol Cell 53 (5), 726-737 (2014)
   PUBMED   24560927
REFERENCE   6  (bases 1 to 2348)
  AUTHORS   Sancak,Y., Markhard,A.L., Kitami,T., Kovacs-Bogdan,E., Kamer,K.J.,
            Udeshi,N.D., Carr,S.A., Chaudhuri,D., Clapham,D.E., Li,A.A.,
            Calvo,S.E., Goldberger,O. and Mootha,V.K.
  TITLE     EMRE is an essential component of the mitochondrial calcium
            uniporter complex
  JOURNAL   Science 342 (6164), 1379-1382 (2013)
   PUBMED   24231807
REFERENCE   7  (bases 1 to 2348)
  AUTHORS   Plovanich,M., Bogorad,R.L., Sancak,Y., Kamer,K.J., Strittmatter,L.,
            Li,A.A., Girgis,H.S., Kuchimanchi,S., De Groot,J., Speciner,L.,
            Taneja,N., Oshea,J., Koteliansky,V. and Mootha,V.K.
  TITLE     MICU2, a paralog of MICU1, resides within the mitochondrial
            uniporter complex to regulate calcium handling
  JOURNAL   PLoS One 8 (2), e55785 (2013)
   PUBMED   23409044
REFERENCE   8  (bases 1 to 2348)
  AUTHORS   Tsai,L.C., Chan,G.C., Nangle,S.N., Shimizu-Albergine,M.,
            Jones,G.L., Storm,D.R., Beavo,J.A. and Zweifel,L.S.
  TITLE     Inactivation of Pde8b enhances memory, motor performance, and
            protects against age-induced motor coordination decay
  JOURNAL   Genes Brain Behav 11 (7), 837-847 (2012)
   PUBMED   22925203
REFERENCE   9  (bases 1 to 2348)
  AUTHORS   Cros,N., Tkatchenko,A.V., Pisani,D.F., Leclerc,L., Leger,J.J.,
            Marini,J.F. and Dechesne,C.A.
  TITLE     Analysis of altered gene expression in rat soleus muscle atrophied
            by disuse
  JOURNAL   J Cell Biochem 83 (3), 508-519 (2001)
   PUBMED   11596118
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF327513.1, EV765275.1, BQ207114.1, DY317464.1, EX491796.1 and
            CV797964.1.
            
            On Oct 26, 2007 this sequence version replaced NM_134396.1.
            
            ##Evidence-Data-START##
            CDS exon combination :: AF327513.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMD00132261,
                                    SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                AF327513.1         1-38
            39-266              EV765275.1         46-273
            267-707             BQ207114.1         35-475              c
            708-1033            DY317464.1         358-683
            1034-1681           EX491796.1         49-696
            1682-2348           CV797964.1         1-667               c
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     gene            1..2348
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /note="mitochondrial calcium uptake 2"
                     /db_xref="GeneID:171433"
                     /db_xref="RGD:619739"
     exon            1..221
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     CDS             12..1307
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /note="EF-hand domain-containing family member A1; EF hand
                     domain family A1; EF-hand domain family, member A1"
                     /codon_start=1
                     /product="calcium uptake protein 2, mitochondrial"
                     /protein_id="NP_599223.2"
                     /db_xref="GeneID:171433"
                     /db_xref="RGD:619739"
                     /translation="MAAAAGRSARLAAWGERLRRGLAAGGRAVPSRGPLAAAVAGVAL
                     AGAGAAWHHGRVKAAARESTLTGLVQKNDLGPIEKLSLRKQRFMQFSSLEHDGEYYMT
                     PRDFLFSVMFERMDRKTLVKKLAKKDIEDVLSGIQTARCGSTFFRDLGDKGVISYTEY
                     LFLLTILTKPHSGFHVAFKMLDADGNEMIERKEFVKLQKIISKQDGFKTVKTNEIEYQ
                     EPTVNEPGLNTTLQVRFFGKRGEKKLHYKEFRRFMENLQTEVQEMEFLQFSKGLNFMR
                     KEDFAEWLLFFTNSENKDIYWKNVREKLSVGESISLDEFKSFCHFTTHLEDFAIAMQM
                     FSLAHRPVRLAEFKRAVKVATGQELSNNLLDTVFKIFDLDGDECLSHGEFLGVLKNRM
                     HRGLWVSQQQSVQEYWKCVKKESIKGVKEAWKQAGKGPF"
     exon            222..360
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            361..392
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            393..468
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            469..516
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            517..599
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            600..665
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            666..763
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            764..935
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1044
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1202
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..2333
                     /gene="Micu2"
                     /gene_synonym="Efha1; RGD1309934; Smhs2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cttccgcaaa gatggcggcg gctgcgggaa gaagcgctcg gctggcggcc tggggcgaaa
       61 ggctgcggcg cgggcttgcg gcgggcggac gagctgtgcc gagtcgcggc cctctggcag
      121 cggctgtggc cggcgtggcc ctggcaggcg caggagcagc atggcatcac ggacgcgtga
      181 aagcagccgc gcgcgagagc acccttacgg ggttggtgca gaaaaatgac ttgggaccaa
      241 tagagaaact ttctctccgc aaacagcggt tcatgcagtt ttcctcactg gagcatgacg
      301 gagagtatta catgacgccc cgagacttcc tcttctcagt gatgtttgag cgaatggatc
      361 gtaaaacact agtcaagaag ctggcaaaaa aggatatcga ggatgtactg tcaggaatcc
      421 aaacagcacg ttgtggatca acgtttttta gagacctagg cgataaaggg gtaatttcat
      481 atactgagta tcttttcttg cttacgatcc tcacaaagcc tcactctggg ttccatgttg
      541 cttttaaaat gctggatgca gatggtaatg agatgattga gagaaaggag tttgttaagc
      601 tgcagaagat cataagtaag caagatggct tcaagacagt gaagactaat gagatagaat
      661 accaggaacc aacagtgaac gagcctggcc ttaacacaac ccttcaagtg cgtttctttg
      721 ggaaaagagg agaaaaaaag cttcattata aagaatttcg aagatttatg gaaaatttac
      781 aaacagaagt tcaagaaatg gaattccttc agttttctaa aggtttgaat ttcatgagaa
      841 aagaagactt tgcagagtgg ctacttttct tcactaactc tgaaaataaa gacatttact
      901 ggaaaaatgt gagagaaaag ttgtcagtag gagagagcat tagtttggat gagttcaagt
      961 ccttctgcca ttttacaaca catttggaag acttcgctat cgccatgcag atgttcagtt
     1021 tagctcatcg ccctgtgagg ctggcggagt tcaagagagc tgtgaaagtg gcgactggac
     1081 aagagctttc caacaatctt ctggacactg tcttcaagat ctttgacctg gacggggacg
     1141 aatgcctcag ccacggggag tttcttggag tactgaaaaa caggatgcat cgaggtttat
     1201 gggtgtcgca gcagcagagt gtgcaagaat actggaagtg cgtgaagaag gaaagcatta
     1261 agggagtgaa ggaggcctgg aaacaggccg ggaagggccc tttctgaaag acgtaacagc
     1321 tcacggggcc gggatagttc tcaaagaact ggctggtttt ccataagtct tcattgagtt
     1381 gccgtgcgga taaagacgcc atgtactcat tttctgactt gggggttttg gagggatgag
     1441 gggaggtgaa taattgtgtg ccacagtagg aagtcagagg ccaacctgtg ggagccggtt
     1501 ctccatggga gtccagagat tgaactcagg ttatgagctt tggtggcaag tgccttcacc
     1561 tgataagcca tctcaccagc gctgactcgg taatgtcttg gtgaattttc attgacaaag
     1621 tattcttcta tgaaaacatg cactgctctg ttaaaggccc gaatgcaaag ggatgcttta
     1681 ggaagccgaa ggccaaggct ggcactgcgt gctttggaca tgagaatgca gtgtgctttc
     1741 tggagggcta aacaagtcca ccagggaagg gacatcgttc ccaagacagg tatcgatccc
     1801 agaagccatg gtggcctagg gagtagttga agttaacact tgcttcggtg tttctacctt
     1861 gaaaataaca tgtaagtgac agtgtcctgt ctggaagcag gataaagtgt ttacattgat
     1921 acaaaaagaa ttatttcttt tataaatttc catgcttttg aaatataagt atagtgtcct
     1981 ctcccagata cataggtgtg ggggctcaac cagcatagga catagctatg taccatactt
     2041 tgctaacaag cagcagacag ttcaatctcc agccactcgt gcacacagat acacacacac
     2101 tgatcataat atcaaggttt cataaggtaa ggtgtcttgg gttgtgggaa acattcagta
     2161 tggcactata aagttgtttg caataaaata aatttgaaat aatgttatgt ggaaaagtat
     2221 gtatatgttt ctgtatacag gtgggcacac gtatgtgtgg aagcccctgt tcctgtaaat
     2281 aacccccatg tttctgcagt catccaataa acccattgat tcaccaaact agaaaaaaaa
     2341 aaaaaaaa
//
