LOCUS       NM_001419564            3304 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus MICAL-like 2 (Micall2), mRNA.
ACCESSION   NM_001419564 XM_006248975
VERSION     NM_001419564.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Sun,Y., Jaldin-Fincati,J., Liu,Z., Bilan,P.J. and Klip,A.
  TITLE     A complex of Rab13 with MICAL-L2 and alpha-actinin-4 is essential
            for insulin-dependent GLUT4 exocytosis
  JOURNAL   Mol Biol Cell 27 (1), 75-89 (2016)
   PUBMED   26538022
  REMARK    GeneRIF: Findings suggest that MICAL-L2 is an effector of
            insulin-activated Rab13, which links to GLUT4 through ACTN4,
            localizing GLUT4 vesicles at the muscle cell periphery to enable
            their fusion with the membrane.
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Hasson,S.A., Kane,L.A., Yamano,K., Huang,C.H., Sliter,D.A.,
            Buehler,E., Wang,C., Heman-Ackah,S.M., Hessa,T., Guha,R.,
            Martin,S.E. and Youle,R.J.
  TITLE     High-content genome-wide RNAi screens identify regulators of parkin
            upstream of mitophagy
  JOURNAL   Nature 504 (7479), 291-295 (2013)
   PUBMED   24270810
REFERENCE   3  (bases 1 to 3304)
  AUTHORS   Sakane,A., Alamir Mahmoud Abdallah,A., Nakano,K., Honda,K.,
            Kitamura,T., Imoto,I., Matsushita,N. and Sasaki,T.
  TITLE     Junctional Rab13-binding protein (JRAB) regulates cell spreading
            via filamins
  JOURNAL   Genes Cells 18 (9), 810-822 (2013)
   PUBMED   23890175
REFERENCE   4  (bases 1 to 3304)
  AUTHORS   Sakane,A., Honda,K. and Sasaki,T.
  TITLE     Rab13 regulates neurite outgrowth in PC12 cells through its
            effector protein, JRAB/MICAL-L2
  JOURNAL   Mol Cell Biol 30 (4), 1077-1087 (2010)
   PUBMED   20008558
REFERENCE   5  (bases 1 to 3304)
  AUTHORS   Nakatsuji,H., Nishimura,N., Yamamura,R., Kanayama,H.O. and
            Sasaki,T.
  TITLE     Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to
            cell-cell junctions and the formation of functional tight junctions
  JOURNAL   Mol Cell Biol 28 (10), 3324-3335 (2008)
   PUBMED   18332111
REFERENCE   6  (bases 1 to 3304)
  AUTHORS   Yamamura,R., Nishimura,N., Nakatsuji,H., Arase,S. and Sasaki,T.
  TITLE     The interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates
            the assembly of tight junctions and adherens junctions
  JOURNAL   Mol Biol Cell 19 (3), 971-983 (2008)
   PUBMED   18094055
REFERENCE   7  (bases 1 to 3304)
  AUTHORS   Terai,T., Nishimura,N., Kanda,I., Yasui,N. and Sasaki,T.
  TITLE     JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the
            endocytic recycling of occludin
  JOURNAL   Mol Biol Cell 17 (5), 2465-2475 (2006)
   PUBMED   16525024
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000012.1.
            
            On Apr 11, 2023 this sequence version replaced XM_006248975.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643288.47035.1,
                                           SRR26360172.1562835.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-281               JAXUCZ010000012.1  20013037-20013317
            282-330             JAXUCZ010000012.1  20027140-20027188
            331-472             JAXUCZ010000012.1  20029259-20029400
            473-666             JAXUCZ010000012.1  20030211-20030404
            667-782             JAXUCZ010000012.1  20031044-20031159
            783-1931            JAXUCZ010000012.1  20031716-20032864
            1932-2188           JAXUCZ010000012.1  20034979-20035235
            2189-2267           JAXUCZ010000012.1  20035872-20035950
            2268-2425           JAXUCZ010000012.1  20036545-20036702
            2426-2569           JAXUCZ010000012.1  20037551-20037694
            2570-2634           JAXUCZ010000012.1  20037808-20037872
            2635-2758           JAXUCZ010000012.1  20037947-20038070
            2759-2849           JAXUCZ010000012.1  20038524-20038614
            2850-2923           JAXUCZ010000012.1  20038699-20038772
            2924-3038           JAXUCZ010000012.1  20039277-20039391
            3039-3085           JAXUCZ010000012.1  20041415-20041461
            3086-3304           JAXUCZ010000012.1  20041577-20041795
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12q11"
     gene            1..3304
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /note="MICAL-like 2"
                     /db_xref="GeneID:288515"
                     /db_xref="RGD:1307875"
     exon            1..281
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..3159
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /note="Junctional Rab13-binding protein; Molecule
                     interacting with CasL-like 2"
                     /codon_start=1
                     /product="mICAL-like protein 2"
                     /protein_id="NP_001406493.1"
                     /db_xref="GeneID:288515"
                     /db_xref="RGD:1307875"
                     /translation="MAAIKALQEWCRQQCEGYRDVSITNMTTSFRDGLAFCAILHRHR
                     PDLINFNALRKENIYENNKLAFQVAEEQLGIPALLDAEDMVALKIPDRLSILTYVSQY
                     YNYFHGRSPIGGMAGMKRPSSDSTEELSGKKKVPSQPAKLSSPVPTQRLPLSPARTNP
                     VVQRNEGVSERPSPKAAPGTVGSSVSSICGVCGKHVHLVQRHLADGRLYHRSCFRCKQ
                     CSSTLHSGAYRATGEPGVFVCTHHSSEAVSVSPKLSNLASRQPGGGIADTRPIGVSQK
                     VLETNGEATPLRARTAAWEHAGGNRAAKGFVQTELTPPATSRVHVGSPAGPRLPMSTV
                     TTTSANSKATTHVTNSSPVGWSSSAQSSTGTSGSRPVVSPSALGAHLSVPQGQAASKG
                     VKTQLNSSTDSSSTAPTPAWTSSSSRTQQAREKFFHNLSPAPAPAPASSSSSHASRVP
                     TVVTAPSGKVSPLVNTSTSKVPSATVVTVPTSKASTVVTAPTSKAPTVVTVPISKAPT
                     VVTAPTSKVSTVVTVPTSKASTVVTAPTSKASTVVTVPTGRGHVVVNTSASKVSGVVD
                     NPAQESSREQALSVLRKALPGLTRAGSQAPSRSSPATSSVLITLPKNEVPPKVPSAKL
                     SHSTTQAFSPTPKMEPTAPLSVGSTSWTSVSLQAGKKSPGISPGIGKTSAVSRPQAEV
                     KGPGPTSQEGQEEGPEGWRARLKPVDKRALEQKEPVLAEPRAGDTPRKASSSSDSSIH
                     ITLTPIQQKRTPCLADSGSSLAAPSPPSRRKKLVVPPTLDVSADWLQPELKKQDDQTR
                     SCKEKTATWGTRESSAILDNDLVSPDEAVTSPVRLHPNYISQEELQRQLQDIERQLDA
                     LELRGVELEKRLRAAEGDASEDGLMVDWFRLIHEKQLLLRRESELMYKSKDQCLEERQ
                     LDLQGELRRLMEKPEGLKSPQDRKREQELLNQYVNTVNDRSDIVDNLDEDRLREQEED
                     QMLESMIQNLGLQRKKSKSFLSKIWSSKSKSGQT"
     exon            282..330
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            331..472
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            473..666
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            667..782
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            783..1931
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            1932..2188
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            2189..2267
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            2268..2425
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            2426..2569
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            2570..2634
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            2635..2758
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            2759..2849
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            2850..2923
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            2924..3038
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            3039..3085
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
     exon            3086..3304
                     /gene="Micall2"
                     /gene_synonym="Jrab; MICAL-L2; RGD1307875"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtgtggggc gccggtctgc tggagctctc ttcttccagc ccggctggtg gtcggcgggt
       61 ccgggcctcc tctgcgtctc gggccctagc agggcctgtg gccagctcct cgagccgcca
      121 gcccgtcttg ccgcccgcat ggccgccatc aaagccctgc aagaatggtg ccggcagcag
      181 tgcgagggct accgggacgt gagcatcacc aacatgacta cctcgttccg cgatggcctg
      241 gccttctgcg ctatcttgca ccgacaccgg ccagacctta taaacttcaa tgccctccgg
      301 aaggaaaaca tttacgagaa caacaagctg gccttccaag tggctgagga acagctgggc
      361 atcccagccc tgctggacgc tgaggacatg gttgccttga agattccaga ccggctgagc
      421 atcctcacct atgtgtcaca gtattacaat tacttccacg gccggtcccc aattggaggc
      481 atggctggca tgaagagacc ctcatcagac tcgactgaag aactttctgg aaagaagaag
      541 gtcccgtctc agccggccaa gttgtcctcg cctgttccaa cccagaggtt accgttgtcc
      601 ccagctagga caaaccccgt ggttcagagg aatgaaggtg tctcagagag accatcccca
      661 aaggccgctc cggggactgt gggaagctct gtcagcagca tctgcggggt ctgtggcaag
      721 cacgttcacc tcgtgcagcg gcacctggct gacgggcgcc tctaccaccg aagctgcttc
      781 aggtgtaaac agtgttccag cacgctgcat tcgggggcct accgcgccac gggagagccc
      841 ggcgtctttg tgtgcactca tcacagctca gaagccgtct ctgtgagccc caagttgtca
      901 aacctggcct ccaggcagcc aggaggtggc atcgcagaca ccaggccaat cggagtctca
      961 cagaaggttc tggagacaaa tggagaggcg acaccactga gggccaggac agcagcctgg
     1021 gagcacgccg gaggcaacag agctgccaaa ggttttgtcc agactgaact tacgccgcca
     1081 gctacctccc gcgtccatgt ggggagccct gcagggccca ggctacctat gagtacggtg
     1141 actactactt ctgcgaatag caaagcgacc acccatgtga ctaacagctc cccagtggga
     1201 tggtcatcat ctgcccagag cagcacaggg acctccggct cccgccctgt tgtatcccca
     1261 agtgctctag gcgctcatct atctgtgccc caaggccaag cagcttcaaa aggtgttaag
     1321 actcaactca actccagcac agactcttca agcacagcgc ccaccccagc ctggacctcc
     1381 tcatcctcta ggactcagca ggcccgagag aaatttttcc acaacctttc tccagcccca
     1441 gccccagccc cagccagtag cagcagcagt catgccagca gggtccccac tgtggtgact
     1501 gcccccagtg gcaaggtttc ccctctggtg aatacctcta ccagcaaggt cccctctgcc
     1561 actgtggtga ctgtccccac cagcaaggcc tccactgtgg tgactgcccc caccagcaag
     1621 gcccccactg tggtgactgt tcccatcagc aaggccccca ctgtggtgac tgcccctaca
     1681 agcaaggtct ccactgtggt gactgtcccc accagcaagg cctccactgt ggtgactgcc
     1741 cccaccagca aggcctccac tgtggtgact gtccccactg gtaggggcca tgttgtggtg
     1801 aatacttctg ccagcaaagt ctctggtgtc gtggataacc ctgcccagga gagcagccgg
     1861 gagcaagcac taagtgtcct taggaaggcc ctgccggggc tgaccagagc tggttcccag
     1921 gctccaagca ggtcctcccc agccacttcc tctgtcctga tcacccttcc caagaatgaa
     1981 gtgccaccaa aagttccctc agccaagctg tcacattcaa ccactcaggc ctttagtccc
     2041 acaccaaaga tggagcccac agcgccgctt agtgtgggca gtacctcgtg gacatccgta
     2101 tcactccagg ctggcaaaaa gagcccgggt atttctccag gcattggtaa gaccagcgct
     2161 gtctccaggc cacaagcaga agtaaagggt ccaggtccca cttctcagga aggccaagag
     2221 gaagggccag aaggatggag ggcccggctg aagcctgtgg ataagagggc tctagaacaa
     2281 aaagaaccag tcctggcaga gccgagggca ggggacactc ccaggaaagc ctccagtagc
     2341 tctgactcca gcatccacat cactttaact cccattcagc aaaagaggac accgtgcctg
     2401 gctgattctg ggtccagcct tgcagccccc tctcctccat cacgccgcaa gaaactagtt
     2461 gttcctccca ccctcgatgt ttctgcggat tggctccagc cagagctcaa gaagcaggac
     2521 gatcagacca ggagctgtaa ggagaagaca gccacctggg ggacaagaga gagctctgca
     2581 atcctggaca atgaccttgt ttcacctgac gaggctgtga cctccccagt caggctccac
     2641 cccaactata tctcccagga agagctgcag agacagctgc aggacattga gagacaactg
     2701 gacgccctgg agctcagggg tgttgagttg gagaagcggc tgcgggcagc tgagggagat
     2761 gcttctgagg atggcctcat ggtggactgg ttccggctta ttcacgagaa gcagttgctg
     2821 ctgaggcgtg aatcggaact catgtacaag tccaaggacc agtgcctgga ggagcggcag
     2881 ctagacctcc agggtgagct gcgtagactg atggagaagc cagagggtct gaagtcccct
     2941 caggaccgca agcgggagca ggagctgctg aaccaatatg tgaatactgt gaatgaccga
     3001 agtgacatcg ttgacaacct ggatgaggat cggctccggg aacaggagga ggaccaaatg
     3061 ctggagagta tgatccagaa tctgggcctc cagagaaaga agtccaagtc cttcttgtcc
     3121 aagatctggt cctcgaagag caaaagtgga cagacatgag ctgcacatgg ctggcacaag
     3181 ggccctggcc cttctcagga agagactggt gctgtgactc cagaagggat gcttgagggt
     3241 accagggccc atggctccca ctctgggtcc ttgtaacctc aataaagaca ttgaccttct
     3301 ctca
//
