LOCUS       NM_001005901            3799 bp    mRNA    linear   ROD 26-APR-2024
DEFINITION  Rattus norvegicus MIB E3 ubiquitin protein ligase 2 (Mib2), mRNA.
ACCESSION   NM_001005901 XM_001052967 XM_001052968 XM_002726666 XM_002729580
VERSION     NM_001005901.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Zemoura,K., Trumpler,C. and Benke,D.
  TITLE     Lys-63-linked Ubiquitination of gamma-Aminobutyric Acid (GABA),
            Type B1, at Multiple Sites by the E3 Ligase Mind Bomb-2 Targets
            GABAB Receptors to Lysosomal Degradation
  JOURNAL   J Biol Chem 291 (41), 21682-21693 (2016)
   PUBMED   27573246
  REMARK    GeneRIF: sustained activation of glutamate receptors, a condition
            occurring in brain ischemia that down-regulates GABAB receptors,
            considerably increased the expression of MIB2 and Lys-63-linked
            ubiquitination of GABAB receptors. Interfering with Lys-63-linked
            ubiquitination by overexpressing ubiquitin mutants or GABAB1
            mutants deficient in Lys-63-linked ubiquitination prevented
            glutamate-induced down-regulation of the receptors.
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Ebstein,F., Lange,N., Urban,S., Seifert,U., Kruger,E. and
            Kloetzel,P.M.
  TITLE     Maturation of human dendritic cells is accompanied by functional
            remodelling of the ubiquitin-proteasome system
  JOURNAL   Int J Biochem Cell Biol 41 (5), 1205-1215 (2009)
   PUBMED   19028597
REFERENCE   3  (bases 1 to 3799)
  AUTHORS   Jurd,R., Thornton,C., Wang,J., Luong,K., Phamluong,K., Kharazia,V.,
            Gibb,S.L. and Ron,D.
  TITLE     Mind bomb-2 is an E3 ligase that ubiquitinates the
            N-methyl-D-aspartate receptor NR2B subunit in a
            phosphorylation-dependent manner
  JOURNAL   J Biol Chem 283 (1), 301-310 (2008)
   PUBMED   17962190
REFERENCE   4  (bases 1 to 3799)
  AUTHORS   Koo,B.K., Yoon,K.J., Yoo,K.W., Lim,H.S., Song,R., So,J.H., Kim,C.H.
            and Kong,Y.Y.
  TITLE     Mind bomb-2 is an E3 ligase for Notch ligand
  JOURNAL   J Biol Chem 280 (23), 22335-22342 (2005)
   PUBMED   15824097
REFERENCE   5  (bases 1 to 3799)
  AUTHORS   Matsuda,A., Suzuki,Y., Honda,G., Muramatsu,S., Matsuzaki,O.,
            Nagano,Y., Doi,T., Shimotohno,K., Harada,T., Nishida,E., Hayashi,H.
            and Sugano,S.
  TITLE     Large-scale identification and characterization of human genes that
            activate NF-kappaB and MAPK signaling pathways
  JOURNAL   Oncogene 22 (21), 3307-3318 (2003)
   PUBMED   12761501
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On or before Sep 1, 2012 this sequence version replaced
            XM_002726666.2, XM_001052968.3, XM_002729580.2, XM_001052967.3,
            NM_001005901.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643298.30683.1,
                                           SRR26643288.25153.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-682               JAXUCZ010000005.1  171541229-171541910 c
            683-839             JAXUCZ010000005.1  171540079-171540235 c
            840-1110            JAXUCZ010000005.1  171532218-171532488 c
            1111-1282           JAXUCZ010000005.1  171531962-171532133 c
            1283-1389           JAXUCZ010000005.1  171530950-171531056 c
            1390-1584           JAXUCZ010000005.1  171530654-171530848 c
            1585-1727           JAXUCZ010000005.1  171530389-171530531 c
            1728-1823           JAXUCZ010000005.1  171530159-171530254 c
            1824-1928           JAXUCZ010000005.1  171529133-171529237 c
            1929-2091           JAXUCZ010000005.1  171528876-171529038 c
            2092-2225           JAXUCZ010000005.1  171528667-171528800 c
            2226-2374           JAXUCZ010000005.1  171528444-171528592 c
            2375-2531           JAXUCZ010000005.1  171528192-171528348 c
            2532-2692           JAXUCZ010000005.1  171527926-171528086 c
            2693-2819           JAXUCZ010000005.1  171527676-171527802 c
            2820-3053           JAXUCZ010000005.1  171527355-171527588 c
            3054-3232           JAXUCZ010000005.1  171526877-171527055 c
            3233-3420           JAXUCZ010000005.1  171526603-171526790 c
            3421-3486           JAXUCZ010000005.1  171526460-171526525 c
            3487-3799           JAXUCZ010000005.1  171526037-171526349 c
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..3799
                     /gene="Mib2"
                     /note="MIB E3 ubiquitin protein ligase 2"
                     /db_xref="GeneID:474147"
                     /db_xref="RGD:1359469"
     exon            1..682
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            683..839
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            840..1110
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     CDS             864..3725
                     /gene="Mib2"
                     /EC_number="2.3.2.27"
                     /note="RBSC-skeletrophin/dystrophin-like polypeptide; mind
                     bomb homolog 2; mindbomb E3 ubiquitin protein ligase 2;
                     RING-type E3 ubiquitin transferase MIB2"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase MIB2"
                     /protein_id="NP_001005901.2"
                     /db_xref="GeneID:474147"
                     /db_xref="RGD:1359469"
                     /translation="MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGS
                     PSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGM
                     RWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQG
                     AKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGK
                     VDLKCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQ
                     EGHGGWNPRMAEMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVID
                     DLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPACLVACR
                     PEEDANLGVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRR
                     HPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASVDLLDEEGNTALHYTAMGNQPE
                     ATRLLLSAGCGVDAQNGTRSTALHVAVQRGFLEVVKILCEHGCDVNLPDAHADTPLHS
                     AISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVD
                     AKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPL
                     LVDAGCNVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGST
                     ELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGG
                     GVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLVLFSPCQHRTVCEECAR
                     RMKKCIRCQVIISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICID
                     SHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV"
     exon            1111..1282
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1389
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1584
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            1585..1727
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..1823
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1928
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2091
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            2092..2225
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            2226..2374
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            2375..2531
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            2532..2692
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            2693..2819
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            2820..3053
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            3054..3232
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            3233..3420
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            3421..3486
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
     exon            3487..3799
                     /gene="Mib2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggagttgtc tgccccgccc ccgggctgtc tctgcaaact acagctcccg gcagtctgtg
       61 cggcggcccg cgaccgtggt cggggggccg tggtggggct ccctggtggg ctagcgctgg
      121 agaggcggcc ttgctaagca ctgttggggg gcggaggacg gcgggctgca gctggcgcct
      181 cggtggcaag ccgaggttcc actgtcagcc tctgcgctgc tcagagccgg gtattcctgg
      241 gccgaacgag cgcggaaact ccgggcaagt ggggggccgg gccgggcgcc ctcccagcgc
      301 cgggggcggt ggcacggggc ctaggggggc ctgatcatcc tgacgaggct gaggtcttgt
      361 cggccgacct cggcccttca gcacgaccgg cttttagtcc ttcccccccg gacccagctc
      421 gtgcccgttg cgcgttctcg gtgcgtgggg gtgatgcagc gtgtcccagc caccagaccc
      481 atgggagctt tttaaggcga ccagcggcct ctgtcccttc tcagatgtta actgcgtctc
      541 agcacccctg gccatagggt tggatctccg ggggctttca gaaaggaagg tcagcacggc
      601 ggagcccagt cccaagtctc caagcatcag ggcttcctgc ccgattctca aggcgggtgg
      661 gggacactgg acctccagac agcctggctc cctgccttcc tgtttggttt gctgtattct
      721 atgttggcaa tggctgctag tttcccacag gaagttttgg cccaggaagc aaatttgtgg
      781 cccaggaagt agcaactaaa tgcctgggat gctgactaga ggcttctttg tgttctaagg
      841 tctttgaaca accccccctc accatggacc tggaccccca tgcaggtgtg caggtgggca
      901 tgcgtgtggt acgcggaatg gactggaaat ggggccagca ggatggaggt gagggcggtg
      961 tgggcaccgt ggtggagctt ggccgccatg gcagcccctc gacccccgac cgtacagttg
     1021 tcgttcagtg ggaccagggc acacgtacca actatcgagc tggctaccaa ggtgcccatg
     1081 acctgttgct ctatgacaac gcccaaatcg gtatccgcca tcccaacatc atctgtgact
     1141 gttgcaagaa acatgggctt cgtggcatgc gttggaagtg ccgtgtctgc tttgactatg
     1201 atctctgcac gcagtgctac atgcacaaca aacatgacct tacccatgcc ttcgagcgct
     1261 atgagacatc tcactctcgc ccggttacgc tgagtccccg acagggcctc cctcgaatcc
     1321 cactgagggg catctttcaa ggagcaaaag tggtacgagg ccctgactgg gaatggggct
     1381 cacaagatgg aggggaaggc aagacaggcc gtgtggtgga catccgtggc tgggatgtgg
     1441 agacaggccg aagtgtggcc agtgtgacat gggcagatgg aaccacaaat gtgtaccgag
     1501 tgggccacaa aggcaaggtg gatctcaaat gtgttggtga ggcagctggc ggcttttact
     1561 acaaggagca cctcccgaag cttggcaagc cagcagagct acagcgcagg gtgagtgctg
     1621 atggccagcc cttccagcgt ggggacaagg tcaagtgtct gctggacaca gatgtcctaa
     1681 gggacatgca agaaggccat ggtggctgga accctaggat ggcagagatg ggcaccgtgc
     1741 accgcatcac agaccgtggg gacgtgcgtg tgcagttcaa ccatgaaacc cgctggacct
     1801 tccaccctgg ggctctcacc aagcacaaca gcttctgggt aggtgatgtg gtccgggtca
     1861 tcgatgacct tgacactgtg aagcgactgc aagctggaca tggagaatgg actgacgaca
     1921 tggcccccgc cctgggccga gtggggaaag tggtgaaggt gtttggagat ggaaacttgc
     1981 gtgtggcggt tggtggccag cggtggacct tcagccccgc ctgcctagtg gcctgccggc
     2041 ctgaggagga tgccaacctg ggcgtggctg agcgcgccag agagaacaaa agctcactga
     2101 gtgtggccct ggacaagctt cggacacaga agagcgaccc agagcaccca gggaggctgg
     2161 tagtggaggc tgcactggga aatgtagccc gggccctgga cctgcttcga aggcatcctg
     2221 agcaggtgga caccaagaac cagggtagga ctgccctgca ggtggctgct tacctgggcc
     2281 aggtagaact ggtacggctg ctgctgcagg caagagcaag tgtggacctg ctggatgagg
     2341 agggcaacac tgcccttcac tacacagcca tggggaacca gcctgaggcc acaaggctgc
     2401 tcctgagtgc cggatgtggg gtggatgctc agaatggcac acgcagcaca gccctacacg
     2461 tggctgtaca gaggggcttc ctagaggtgg taaagatcct gtgtgaacat ggttgtgatg
     2521 tcaacttgcc ggatgcccat gcagacacac ccctgcattc tgccatctct gcgggtgccg
     2581 gtgccagcag cattgttgaa gtcctcaccg aggtgcctgg catcgatgtc actgctacca
     2641 atagccaggg cttcacactg ctgcaccatg catccctcaa gggccatgtg ctagcagtta
     2701 gaaagattct ggcaagagca cggcagctgg tggatgccaa gaaggaggac ggctttactg
     2761 cgctacatct ggcagctctt aacaaccacc gtgaggtggc ccaggtccta atccgagagg
     2821 gccgctgcga tgtgaatgtg cgaaacagga agcttcaatc cccactgcat ctggctgtgc
     2881 agcaggccca cctggggctc gtaccgctgc tggtggacgc tggctgcaat gtcaacactg
     2941 aggatgagga gggtgacaca gccctgcatg tggcactaca gcgtcatcag ctgctgcccc
     3001 tggtggctga cagggctggg ggagacccag ggcccttgca gctgctgtca aggttacagg
     3061 cctcaggcct cccaggcagc acagagttga ctgtaggcgc cgcagtggcc tgcttcctgg
     3121 cattggaggg tgctgatgtg agctacgcaa accaccgcgg ccggagccca ctggacctgg
     3181 ccacagaagg ccgagtgctc aaggccttgc agggctgtgc ccagcgcttc agggagcgac
     3241 aggcaggtgg cggtgggggt gtgcccccgg gcccccggca tgtgctgagt actcctaaca
     3301 ccgtgacgaa cctgcatgtg tctggcacag cagggccaga agctgccgag tgcctggtgt
     3361 gctcggagct ggcattgcta gttctgttct caccgtgtca gcaccgcaca gtgtgtgagg
     3421 agtgcgcacg aaggatgaag aagtgtatca ggtgccaggt gatcatcagc aagaagcttc
     3481 gcccagatgg ttcagaggtg gttaatgcca tccaggtgcc cggcccgcct cggcaactgg
     3541 tagaggagct acagagtcgc tacaggcaga tggaggagcg catcacctgc cccatctgca
     3601 tcgacagcca catccgcctg gtgttccagt gcggccatgg agcatgtgcg ccctgtggcg
     3661 ctgcccttaa cgcctgcccc atttgccgcc agcccatccg tgaccgcatt cagatcttcg
     3721 tgtgagcgca ctacacacgc tgtggctgag cccaggctct ccttcaaaac tcctgtattt
     3781 tataaaaaga ttatcggac
//
