LOCUS       NM_012861                812 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Rattus norvegicus O-6-methylguanine-DNA methyltransferase (Mgmt),
            mRNA.
ACCESSION   NM_012861
VERSION     NM_012861.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Ni,Y., Gu,J., Wu,J., Xu,L. and Rui,Y.
  TITLE     MGMT-Mediated neuron Apoptosis in Injured Rat Spinal Cord
  JOURNAL   Tissue Cell 62, 101311 (2020)
   PUBMED   32433023
  REMARK    GeneRIF: MGMT-Mediated neuron Apoptosis in Injured Rat Spinal Cord.
REFERENCE   2  (bases 1 to 812)
  AUTHORS   Quintavalle,C., Mangani,D., Roscigno,G., Romano,G.,
            Diaz-Lagares,A., Iaboni,M., Donnarumma,E., Fiore,D., De Marinis,P.,
            Soini,Y., Esteller,M. and Condorelli,G.
  TITLE     MiR-221/222 target the DNA methyltransferase MGMT in glioma cells
  JOURNAL   PLoS One 8 (9), e74466 (2013)
   PUBMED   24147153
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 812)
  AUTHORS   Ling,Z.Q., Li,P., Ge,M.H., Hu,F.J., Fang,X.H., Dong,Z.M. and
            Mao,W.M.
  TITLE     Aberrant methylation of different DNA repair genes demonstrates
            distinct prognostic value for esophageal cancer
  JOURNAL   Dig Dis Sci 56 (10), 2992-3004 (2011)
   PUBMED   21674174
REFERENCE   4  (bases 1 to 812)
  AUTHORS   Toshimitsu,H., Yoshimoto,Y., Augustine,C.K., Padussis,J.C.,
            Yoo,J.S., Angelica Selim,M., Pruitt,S.K., Friedman,H.S.,
            Ali-Osman,F. and Tyler,D.S.
  TITLE     Inhibition of poly(ADP-ribose) polymerase enhances the effect of
            chemotherapy in an animal model of regional therapy for the
            treatment of advanced extremity malignant melanoma
  JOURNAL   Ann Surg Oncol 17 (8), 2247-2254 (2010)
   PUBMED   20182810
REFERENCE   5  (bases 1 to 812)
  AUTHORS   Duthie,S.J., Grant,G., Pirie,L.P., Watson,A.J. and Margison,G.P.
  TITLE     Folate deficiency alters hepatic and colon MGMT and OGG-1 DNA
            repair protein expression in rats but has no effect on genome-wide
            DNA methylation
  JOURNAL   Cancer Prev Res (Phila) 3 (1), 92-100 (2010)
   PUBMED   20051376
  REMARK    GeneRIF: Folate deficiency resulted in significant increases in
            levels of the DNA repair proteins OGG-1 and MGMT in the liver
REFERENCE   6  (bases 1 to 812)
  AUTHORS   Liem,L.K., Lim,A. and Li,B.F.
  TITLE     Specificities of human, rat and E. coli O6-methylguanine-DNA
            methyltransferases towards the repair of O6-methyl and
            O6-ethylguanine in DNA
  JOURNAL   Nucleic Acids Res 22 (9), 1613-1619 (1994)
   PUBMED   8202360
REFERENCE   7  (bases 1 to 812)
  AUTHORS   Santibanez-Koref,M., Elder,R.H., Fan,C.Y., Cawkwell,L., McKie,J.H.,
            Douglas,K.T., Margison,G.P. and Rafferty,J.A.
  TITLE     Isolation and partial characterization of murine
            O6-alkylguanine-DNA-alkyltransferase: comparative sequence and
            structural properties
  JOURNAL   Mol Carcinog 5 (2), 161-169 (1992)
   PUBMED   1554415
REFERENCE   8  (bases 1 to 812)
  AUTHORS   Sakumi,K., Shiraishi,A., Hayakawa,H. and Sekiguchi,M.
  TITLE     Cloning and expresion of cDNA for rat O6-methylguanine-DNA
            methyltransferase
  JOURNAL   Nucleic Acids Res 19 (20), 5597-5601 (1991)
   PUBMED   1945835
REFERENCE   9  (bases 1 to 812)
  AUTHORS   Rahden-Staron,I. and Laval,F.
  TITLE     cDNA cloning of the rat O6-methylguanine-DNA-methyltransferase
  JOURNAL   Biochem Biophys Res Commun 177 (2), 597-602 (1991)
   PUBMED   2049083
REFERENCE   10 (bases 1 to 812)
  AUTHORS   Potter,P.M., Rafferty,J.A., Cawkwell,L., Wilkinson,M.C.,
            Cooper,D.P., O'Connor,P.J. and Margison,G.P.
  TITLE     Isolation and cDNA cloning of a rat
            O6-alkylguanine-DNA-alkyltransferase gene, molecular analysis of
            expression in rat liver
  JOURNAL   Carcinogenesis 12 (4), 727-733 (1991)
   PUBMED   2013136
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from S61804.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FM104063.1, SRR8487230.66046.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132263, SAMD00132264
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q41"
     gene            1..812
                     /gene="Mgmt"
                     /note="O-6-methylguanine-DNA methyltransferase"
                     /db_xref="GeneID:25332"
                     /db_xref="RGD:3087"
     exon            1..61
                     /gene="Mgmt"
                     /inference="alignment:Splign:2.1.0"
     exon            62..198
                     /gene="Mgmt"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..703
                     /gene="Mgmt"
                     /EC_number="2.1.1.63"
                     /note="O-6-methylguanine-DNA-alkyltransferase;
                     6-O-methylguanine-DNA methyltransferase;
                     O6-alkylguanine-DNA alkyltransferase;
                     O(6)-alkylguanine-DNA alkyltransferase;
                     O6-methylguanine-DNA methyltranferase;
                     0-6-methylguanine-DNA methyltransferase"
                     /codon_start=1
                     /product="methylated-DNA--protein-cysteine
                     methyltransferase"
                     /protein_id="NP_036993.1"
                     /db_xref="GeneID:25332"
                     /db_xref="RGD:3087"
                     /translation="MAEICKMKYTVLDSPLGKIELSGCERGLHGIRFLSGKTPNTDPT
                     EAPACPEVLGGPEGVPEPLVQCTAWLEAYFHEPAATEGLPLPALHHPVFQQDSFTRQV
                     LWKLLKVVKFGEMVSYQQLAALAGNPKAARAVGGAMRSNPVPILIPCHRVIRSDGAIG
                     NYSGGGQTVKEWLLAHEGIPTGQPASKGLGLIGSWLKPSFESSSPKPSG"
     exon            199..359
                     /gene="Mgmt"
                     /inference="alignment:Splign:2.1.0"
     exon            360..499
                     /gene="Mgmt"
                     /inference="alignment:Splign:2.1.0"
     exon            500..802
                     /gene="Mgmt"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctcagttcca gagcctgtgt accgttccag gttacggagt ttctgcacag tttgcaaact
       61 ggaacttggc agaatggctg agatctgcaa aatgaaatac acggtgttgg acagcccttt
      121 ggggaagata gagctgtccg gctgtgagcg aggcctgcat gggatacgat ttctcagtgg
      181 gaagacccca aacactgacc ccacagaggc tccagcctgt cctgaggtgc tcggtgggcc
      241 agagggagtg ccagagcccc tggtgcagtg cacagcctgg ctggaagcct atttccacga
      301 acctgcagcc acagaggggc ttcccttgcc tgctctccat caccctgtgt tccagcaaga
      361 ttcattcacc agacaggtgt tatggaagct gctgaaggtt gtgaaattcg gagaaatggt
      421 ttcttaccag caattagcag ccctggcagg caaccccaaa gcggctcgtg cagtaggagg
      481 agcgatgagg agcaatccag tccccatcct catcccctgc cacagggtga ttcgcagtga
      541 cggtgccatt ggcaattact ctggaggagg acagactgtg aaagagtggc ttctggccca
      601 tgagggcatc ccaactggac agccggcctc caaaggcttg ggtctgattg ggagctggct
      661 caagccatcc ttcgagtctt ccagcccaaa gccgtctggc tgaaattgag taaccgtttg
      721 aatgacacat agatgtaatg cggtgttgga agcggatgtg tggtgggtac cactatatta
      781 aaagagctgc atgtgtcctg ggaaaaaaaa aa
//
