LOCUS       NM_001012225            7181 bp    mRNA    linear   ROD 10-JUN-2024
DEFINITION  Rattus norvegicus alpha-1,3-mannosyl-glycoprotein
            4-beta-N-acetylglucosaminyltransferase A (Mgat4a), transcript
            variant 2, mRNA.
ACCESSION   NM_001012225 XM_346039
VERSION     NM_001012225.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Niu,B., Ma,L., Yao,L., Zhang,Y. and Su,H.
  TITLE     HCV affects KATP channels through GnT-IVa-mediated N-glycosylation
            of GLUT2 on the surface of pancreatic beta-cells leading to
            impaired insulin secretion
  JOURNAL   Endocrine 84 (2), 427-440 (2024)
   PUBMED   37962815
  REMARK    GeneRIF: HCV affects KATP channels through GnT-IVa-mediated
            N-glycosylation of GLUT2 on the surface of pancreatic beta-cells
            leading to impaired insulin secretion.
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Kurimoto,A., Kitazume,S., Kizuka,Y., Nakajima,K., Oka,R.,
            Fujinawa,R., Korekane,H., Yamaguchi,Y., Wada,Y. and Taniguchi,N.
  TITLE     The absence of core fucose up-regulates GnT-III and Wnt target
            genes: a possible mechanism for an adaptive response in terms of
            glycan function
  JOURNAL   J Biol Chem 289 (17), 11704-11714 (2014)
   PUBMED   24619415
REFERENCE   3  (bases 1 to 7181)
  AUTHORS   Takamatsu,S., Antonopoulos,A., Ohtsubo,K., Ditto,D., Chiba,Y.,
            Le,D.T., Morris,H.R., Haslam,S.M., Dell,A., Marth,J.D. and
            Taniguchi,N.
  TITLE     Physiological and glycomic characterization of
            N-acetylglucosaminyltransferase-IVa and -IVb double deficient mice
  JOURNAL   Glycobiology 20 (4), 485-497 (2010)
   PUBMED   20015870
REFERENCE   4  (bases 1 to 7181)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   5  (bases 1 to 7181)
  AUTHORS   Ide,Y., Miyoshi,E., Nakagawa,T., Gu,J., Tanemura,M., Nishida,T.,
            Ito,T., Yamamoto,H., Kozutsumi,Y. and Taniguchi,N.
  TITLE     Aberrant expression of N-acetylglucosaminyltransferase-IVa and IVb
            (GnT-IVa and b) in pancreatic cancer
  JOURNAL   Biochem Biophys Res Commun 341 (2), 478-482 (2006)
   PUBMED   16434023
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000009.1, BC088215.1 and BM388934.1.
            
            On May 14, 2009 this sequence version replaced NM_001012225.1.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1. The
            resulting isoform (2) lacks an internal 10-aa segment, compared to
            isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360176.478231.1,
                                           SRR26643299.38878.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                JAXUCZ010000009.1  47262672-47262713   c
            43-1944             BC088215.1         1-1902
            1945-7161           JAXUCZ010000009.1  47169234-47174450   c
            7162-7181           BM388934.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q21"
     gene            1..7181
                     /gene="Mgat4a"
                     /note="alpha-1,3-mannosyl-glycoprotein
                     4-beta-N-acetylglucosaminyltransferase A"
                     /db_xref="GeneID:367252"
                     /db_xref="RGD:1305650"
     exon            1..52
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            53..398
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     CDS             305..1885
                     /gene="Mgat4a"
                     /EC_number="2.4.1.145"
                     /note="isoform 2 is encoded by transcript variant 2;
                     gnT-IVa; glcNAc-T IVa; N-acetylglucosaminyltransferase
                     IVa; N-glycosyl-oligosaccharide-glycoprotein
                     N-acetylglucosaminyltransferase IVa;
                     UDP-N-acetylglucosamine: alpha-1,3-D-mannoside
                     beta-1,4-N-acetylglucosaminyltransferase IVa; mannoside
                     acetylglucosaminyltransferase 4, isoenzyme A; mannosyl
                     (alpha-1,3-)-glycoprotein
                     beta-1,4-N-acetylglucosaminyltransferase, isozyme A"
                     /codon_start=1
                     /product="alpha-1,3-mannosyl-glycoprotein
                     4-beta-N-acetylglucosaminyltransferase A isoform 2"
                     /protein_id="NP_001012225.1"
                     /db_xref="GeneID:367252"
                     /db_xref="RGD:1305650"
                     /translation="MRLRNGTVATALVFVTSFLTLSWYTTWQNGKEKLIAYQREFLAL
                     KERLRVAEHRISQRSSELNTIVQQFRRAGAETNGNNTIKLLKELTSKKSLQVPSIYYH
                     LPHLLQNERSLQPAVQIGSGRTGVSIVMGIPTVKREVKSYLIETLHSLIDNLYPEEKL
                     DCVIVVFIGETDLDYVHSVVANLEKEFSREISSGLLEVISPPESYYPDLTNLKETFGD
                     SKERVRWRTKQNLDYCFLMMYAQEKGIYYIQLEDDIIVKQNYFNTIKNFALQLSSEEW
                     MILEFSQLGFIGKMFQAPDLALVVEFILMFYKEKPIDWLLDHILWVKVCNPEKDAKHC
                     DRQKANLRIRFRPSLFQHVGLHSSLSGKIQKLTDKDYMKPLLLKIHVNPPAEVSTSLK
                     VYQGHTLEKTYMGEDFFWAITPTAGDYILFKFDKPVNVESYLFHSGNQEHPGDILLNT
                     TVEVLPLKSDSLEISKETKDKRLEDGYFRIGKFEYGVAEGIVDPGLNPISAFRLSVIQ
                     NSAVWAILNEIHIKKVTS"
     exon            399..539
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            540..680
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            681..814
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            815..861
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            862..975
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1051
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1166
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1297
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1405
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1599
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..1678
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            1679..1745
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            1746..1858
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..7166
                     /gene="Mgat4a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctccagctg cgtcgccggc ggccgcggta gaagcgccct ggcccgcggc agaaagaaga
       61 aaaagaaaca gcagccctta gccctcagcc tctgaaaacc tcgaagacaa tctggaatag
      121 caatacccac gcttgctttg ctgcttgaca ccttctgccc agcatctgaa gtgaacctca
      181 atcatcaatt gctttgtttt tggcccaggt ccaaggcgtt ctgccttctg ttgacttgag
      241 gctggagaga gactggtctt gcttgctgtt gccaacttgt gaaccagtgt gatggtgaaa
      301 tgagatgagg ctccgaaatg gaactgtggc cactgcgctg gtgtttgtca cgtccttcct
      361 taccctgtcc tggtatacca catggcaaaa tgggaaagaa aaattaattg cttaccaacg
      421 agaattcctc gctttaaaag aacgtcttcg agtggccgaa cataggatat ctcagcgctc
      481 ctctgagcta aacaccattg tccaacagtt ccgccgagct ggagcggaga ctaatggaaa
      541 taataccata aagcttctaa aagagttgac aagcaaaaaa tcacttcaag tgccaagtat
      601 ttattatcat ttgcctcatc tattgcaaaa tgaaagaagc cttcagcccg ccgtacagat
      661 tggcagtgga agaacgggag tttcaatagt catggggatt cctactgtga agagagaagt
      721 taaatcttac ctcatagaga cccttcactc ccttattgat aatctgtatc ctgaagagaa
      781 gctggactgt gttatagttg tcttcatagg agagacagat cttgattatg ttcacagcgt
      841 tgttgccaac ctggagaaag aattttctag agaaattagt tccggcttgc tagaagtaat
      901 ctctcctcct gaaagctatt accccgactt aacaaacctg aaggagacgt tcggagactc
      961 caaggaaaga gtaagatgga gaacgaagca aaacctggac tattgttttc tgatgatgta
     1021 tgctcaggag aagggcattt actacattca gcttgaagat gatattattg tcaagcaaaa
     1081 ctattttaat accataaaaa attttgcact tcaactttct tctgaagaat ggatgattct
     1141 agagttttcc cagctgggct tcattgggaa gatgttccag gcgccggacc tagctctggt
     1201 tgtggagttc attctcatgt tctataagga gaagcccatt gactggctct tggaccacat
     1261 tctctgggtg aaggtctgca accccgaaaa agatgccaaa cactgcgaca gacagaaggc
     1321 aaacctacga atccgcttcc gaccctccct cttccagcac gtgggtctac actcatctct
     1381 gtcggggaaa attcagaaac ttacggataa agattacatg aaaccattgc ttctcaagat
     1441 ccacgtgaac ccgcctgcag aggtctccac ttccctgaag gtgtaccaag ggcacacact
     1501 ggagaagacc tacatggggg aagacttctt ttgggccatc acccccacag ctggagacta
     1561 catcttgttt aaatttgata aaccggtcaa tgtggagagt tatttgttcc acagcggcaa
     1621 tcaagagcac ccaggggaca tcctgctgaa cacgaccgtg gaggttctgc ctcttaagag
     1681 cgacagtttg gagatcagca aagaaaccaa agacaaacga ttagaagatg gctatttcag
     1741 aatagggaaa tttgagtacg gagttgcaga gggaattgtg gatcctggac tgaaccctat
     1801 ctcagccttt cgactgtcgg ttattcagaa ctctgctgtc tgggccattc tcaatgagat
     1861 tcatattaag aaagtcacca gttgatctgc ttagaaacca acacgtcctt tcttgtgact
     1921 cttgattaaa gatactgagc acgccgtcct cttctgctct gacttgaaca ctacctctcg
     1981 tgaagcctac tgtagataag atgattacca tctccacttg ggcagggacc cttcccaccg
     2041 acaactgcat tcatttgaaa cgaggctgtc ctccaaattt gaacttgaca caaacatttt
     2101 acaattctga cagcctggta acacgcctga gactattttg gtattatact aatacacaag
     2161 agttgtacat attgttacat tccttaaaat ttgagaaaac tgatgttaaa tacattttgt
     2221 gaagggggta cttttgaagt tcacttattt tactattata gaccctcttt tatagacgat
     2281 cagggatgtt atatatatat aaatataaat atataaatac acatgaagat gttatgaagt
     2341 taatttatta gaagtactta agaagtacat atttttgtgc agtaaagttt tgaatcaaca
     2401 cctttttttg aagaagcggt tcctggcttt tttaagttcc ttctatattt ttatttgtag
     2461 atgcagacat tctagatcaa cgccattttt ttccagtgca ctgccattcc agattggttc
     2521 tagatgggtt tcttaacttg aagtactttt ataatcacag caattctgaa caaaatattt
     2581 tcagaggcat ttgtcattcc taaaaaaaca atcaagattt taaaagacca gagccctctg
     2641 gagggttata ttactgtact gtgtgtggtg tgtagccaca gaaacccgtg tgattgtcag
     2701 tgtcgggtgg gaggccctgc ctcgtctcca tacactcctt caccccctca tagttccctc
     2761 catgtgggtg acgagactcc tttccctttt gctctgtctt taatcagtag ttagacacac
     2821 tgtcttcttt tctccctggt ccttttatag acctgtggtg ggttttgacg ttcagataat
     2881 gtttcctggg taatgctgtt ggatgagtca tattgggtat aattttacaa ccaacattgt
     2941 tatgtggggc tctggtgaaa gacaaccgta gcgcttctag gtttccacag catgtttgct
     3001 ctgctatgtc ctgaatgcaa gggtgaacgc gtgtgcagtc ccaagaaggg ctagttggca
     3061 aacgtgcttt actcttacac ttaggaaggc cgggcaagtt gccagtcttc tctggctctc
     3121 caggagctgc gcatgcccag caggtgtggg ggcctaggtg tggaagttcc cagctggtgg
     3181 cctccaccct ggaactttct cagttgccca gtgtatggaa cacagtacaa agcagcagcc
     3241 attttgtatg ttctgactgg ggaaacgtag ataaacacta ctgtgcatgt caaagcccag
     3301 ctgaagcttt tcttggtgtt ttgtaaactt tgtaaacatg gtgttttatt caagttttta
     3361 atttcttctc tgatttcctt tgccctgtgc ccacaagcac atccttagaa aatttaagca
     3421 ctatcttccc acactcaagt gtgcagcagg gtaacagact gaaaccatgc ggatggatgc
     3481 aggggccctt ttaggtctct gctttctcag tggctgcaag ttcagagctt tgaaaatcca
     3541 gtgggtcact tgactgtctc ctgcgtgggt ctttgagctg tcctcccgcc aggctcctct
     3601 catttgtctt gcctgtaggg actcttagcc aagtcctttc cttcattcca ggaatccctt
     3661 ttccctttgt gcgcttactc ccagtgccta gaacatgcct gagcctcggc aggttctttt
     3721 agcatctgga tgagtggatt aactagccag gttgctcaca tctctctccg agcactattt
     3781 gcctgctatc ttgtcatgca tgggcacaca cactccgtgt ctagtcttca ttctgtgtgg
     3841 tgtgtgttct ccaacacact ccgagctctg gaggccagct ctcctgtctt ccacgtcagg
     3901 tgctcatttg atccatcagt gttagttgct ctcacttttg tgcccagttc agcactagac
     3961 tatcagaaac agtttggttg gcccaagcaa gatttttgtg ggagccctgg agatttgtga
     4021 aggtaaagac tagaggaggt gaagccagag tacttagaac tgcaaggtgt ccaccttgtg
     4081 ggcaaccaac ggaccttcac ccacagtgaa tgggaaaagt gccaaataca gtaccaatta
     4141 tttctgtgaa gtagatggtg gttggtacca gtgtatttca gatactccag actctagagt
     4201 ggtgctggcc tctacagaag ccacacatct gttgaaatga aatgttgagt tctgtgccca
     4261 tgttggcagc atttggagtg gtcagcggcc acatggccag cagccaccat attggatccc
     4321 agaaatatgg gacactgaca tcatggttct gctggtcata aatataagca ttctagaaag
     4381 gagtgatgca tcctgctgct gtgattaaaa gtagacctat ggattcatgg tagtacaggt
     4441 gaaggtgtgt attttgatgg gacggccaaa attgcctggc tacatgttag cattatgatt
     4501 cttcccagca tgcattgttt cagtgtcccc aagaggtaca tttgaaaagc atgagaagtg
     4561 aatacaggtg gatgatttag tttgtagcct agatgtgtgt tatgtccgtc agagtgtgtt
     4621 tgaacaaatg tcattggtta catgcaaaag attatttcaa aagctgtcag gaaaagttga
     4681 gagattagag ttctttttag tctttcagaa ttacaagaac tatagttctc aacagtgctg
     4741 tctctcaagg aagtttgctt atgtggaaag aggtgttgtt agcaaaggtt tttccagtta
     4801 ataagtgtgt atctgtcata tgcaggattc taagaatgac ccctcccatg actttgtata
     4861 atatcctcct ccccttctga gtatagcgac aactgtgacc atgtcatgtc ttgttactta
     4921 acaaaaggga gattacctgt cagtggctga tccaatgttg agagtcatgt caggctggta
     4981 caagtgagag acagcagcag tagaactctg aacagcattg ctgggcctgc cttaaggagc
     5041 cagaggcttt gttacccact gagtgggagc tgcagacctt ttgccacagg accatttgac
     5101 ccagacactt aaccggtttg gacatggaaa tgcttgggcg agcagtcagc tgggtaaacc
     5161 ttccctgcag ctttgtaagc ggggccctac ctaggcttct gatgtgcagc agtgggtaag
     5221 aaattggcag tgctgtacgt ggctaggttt gtgttttgta aaaagtaaca atctcagtcc
     5281 tgagaaggct tcatatgtga ttgcctgcag tgactggttt ttaagacaag gtctcatctt
     5341 gcaccccagg ctggttctcc cagttctgcc cctcaagtgt cgatgtgcat caccatgccc
     5401 agttgactga tggcttttga cataagaata aacttgttcc tagcaagccc tattcctggc
     5461 tctctgctag gatgcttggt gccaggaccc gtctccacta ctagccagac ttgcgttatt
     5521 ttttgttgtg taaaactggc aggatgaagc tgaaggtgtg ggctgtaggc atcttctcct
     5581 agatactcct aaattgtact gacttaaagg cctgccggaa gtagggggaa gaatgaggaa
     5641 cagtatttta gatcagatca acaagaagaa aaattcccac cgtcatgtcg atttgctggt
     5701 gtgtccaacc ttctgacagt gcagtgggac actgttctct acagagtcca ggctcaagaa
     5761 cctcacggtc cagttacaaa aacaacgaaa ctgttaaaaa gctttgagta agtttacaat
     5821 tttgtattgt gccattcatt gctgtcctgg gccacaggct gaacatacct gcaagagctt
     5881 cagctacgac agctgtaatt tttaaaagat cacttagctg agtagctcac gggtcaaacc
     5941 ctgtatgttc caaggttttg tatttacatg tttcccaaaa taccatgctt attgttgcta
     6001 ttattcaacc atgctgctgt agccttgagg gaaataaaca gaactgaagg aaaggaaccc
     6061 agaggttaaa cactgtcgtc atcttcgcat ggtgagggat cgagtcccag ttcaagactt
     6121 cagggaaggg cattcccaag ccacgtagca acatttacgg cttgtaaaac ttaaactgtg
     6181 atagttgtga ggacttaaat gattaatatt aacatagatg ctatgctaat taatatgcta
     6241 aatttaatgc atgtgaacat atgatgctac aaagatgacc acagtcttta caaagagcaa
     6301 aagaataaaa ccaaccaacc cacccaaggg ttttgttact cgtgcccctg tactctatca
     6361 ctagctaaac agacctttgt tgtttctgaa atgctccctt ttccagtttc tgcactacct
     6421 aaggatgcaa gtgtgagagc agacaggcag caggtggccc tgtgtgagca ggggccggag
     6481 aaggactgga agtgtccgtg caggcacaca ggcggccact gcgagtgcag cgggaacccc
     6541 tggactgcag agggattctt gctgctctca acacatgtgt tgtgggaaca gctaggtact
     6601 tgtgatacta aggaggctgc ttcttgaagc tgctctgctg ctgagttagc aattatccca
     6661 aaatacagag tggggtcctt tagcttgcgt agcattccac cctttattgg aaatgctcca
     6721 gcgctgtgta agaggacctg aagcgcattc tcttgagcct tacaaagagc tgctgggaca
     6781 gtactgtcca gtccactaag ctcacacctc tttcccccag tggagtcaaa gacgctcagt
     6841 tggtgcacgt cctccacaag gctgtcttca cactggggga cgttctggag tcttgctagg
     6901 ttgtaccagg tcctcttttc ctctgctcac aatgtcttta tgggttgaac attaactgca
     6961 gtaatatttg aggtacctgt tgaattttta aaggcagatt atttattttt aagtttgctg
     7021 ctataatcga aacctaataa tgtcctgttg actgtgcctt tcataactgt ctctgtgcta
     7081 caactgtcca tgtgtatttg aaataaagat gtttgaaaag ctatgttgaa tcaagattcc
     7141 ttctaataaa aatggcttaa accaaaaaaa aaaaaaaaaa a
//
