LOCUS       NM_053604               2510 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Rattus norvegicus alpha-1,6-mannosyl-glycoprotein
            2-beta-N-acetylglucosaminyltransferase (Mgat2), mRNA.
ACCESSION   NM_053604
VERSION     NM_053604.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Kadirvelraj,R., Yang,J.Y., Sanders,J.H., Liu,L., Ramiah,A.,
            Prabhakar,P.K., Boons,G.J., Wood,Z.A. and Moremen,K.W.
  TITLE     Human N-acetylglucosaminyltransferase II substrate recognition uses
            a modular architecture that includes a convergent exosite
  JOURNAL   Proc Natl Acad Sci U S A 115 (18), 4637-4642 (2018)
   PUBMED   29666272
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Jin,Y., McFie,P.J., Banman,S.L., Brandt,C. and Stone,S.J.
  TITLE     Diacylglycerol acyltransferase-2 (DGAT2) and monoacylglycerol
            acyltransferase-2 (MGAT2) interact to promote triacylglycerol
            synthesis
  JOURNAL   J Biol Chem 289 (41), 28237-28248 (2014)
   PUBMED   25164810
  REMARK    GeneRIF: The interaction of DGAT2 and MGAT2 serves to channel lipid
            substrates efficiently for triacylglycerol biosynthesis.
REFERENCE   3  (bases 1 to 2510)
  AUTHORS   Voorhees,R.M., Fernandez,I.S., Scheres,S.H. and Hegde,R.S.
  TITLE     Structure of the mammalian ribosome-Sec61 complex to 3.4 A
            resolution
  JOURNAL   Cell 157 (7), 1632-1643 (2014)
   PUBMED   24930395
REFERENCE   4  (bases 1 to 2510)
  AUTHORS   Hassinen,A., Rivinoja,A., Kauppila,A. and Kellokumpu,S.
  TITLE     Golgi N-glycosyltransferases form both homo- and heterodimeric
            enzyme complexes in live cells
  JOURNAL   J Biol Chem 285 (23), 17771-17777 (2010)
   PUBMED   20378551
REFERENCE   5  (bases 1 to 2510)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   6  (bases 1 to 2510)
  AUTHORS   Cormier-Daire,V., Amiel,J., Vuillaumier-Barrot,S., Tan,J.,
            Durand,G., Munnich,A., Le Merrer,M. and Seta,N.
  TITLE     Congenital disorders of glycosylation IIa cause growth retardation,
            mental retardation, and facial dysmorphism
  JOURNAL   J Med Genet 37 (11), 875-877 (2000)
   PUBMED   11228641
  REMARK    Review article
REFERENCE   7  (bases 1 to 2510)
  AUTHORS   Tan,J., Dunn,J., Jaeken,J. and Schachter,H.
  TITLE     Mutations in the MGAT2 gene controlling complex N-glycan synthesis
            cause carbohydrate-deficient glycoprotein syndrome type II, an
            autosomal recessive disease with defective brain development
  JOURNAL   Am J Hum Genet 59 (4), 810-817 (1996)
   PUBMED   8808595
REFERENCE   8  (bases 1 to 2510)
  AUTHORS   Tan,J., D'Agostaro,A.F., Bendiak,B., Reck,F., Sarkar,M.,
            Squire,J.A., Leong,P. and Schachter,H.
  TITLE     The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2-
            N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic
            DNA, localization to chromosome 14q21, expression in insect cells
            and purification of the recombinant protein
  JOURNAL   Eur J Biochem 231 (2), 317-328 (1995)
   PUBMED   7635144
REFERENCE   9  (bases 1 to 2510)
  AUTHORS   D'Agostaro,G.A., Zingoni,A., Moritz,R.L., Simpson,R.J.,
            Schachter,H. and Bendiak,B.
  TITLE     Molecular cloning and expression of cDNA encoding the rat
            UDP-N-acetylglucosamine:alpha-6-D-mannoside
            beta-1,2-N-acetylglucosaminyltransferase II
  JOURNAL   J Biol Chem 270 (25), 15211-15221 (1995)
   PUBMED   7797505
REFERENCE   10 (bases 1 to 2510)
  AUTHORS   Bendiak,B. and Schachter,H.
  TITLE     Control of glycoprotein synthesis. Kinetic mechanism, substrate
            specificity, and inhibition characteristics of
            UDP-N-acetylglucosamine:alpha-D-mannoside beta 1-2
            N-acetylglucosaminyltransferase II from rat liver
  JOURNAL   J Biol Chem 262 (12), 5784-5790 (1987)
   PUBMED   2952645
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC081754.1.
            
            On Sep 16, 2004 this sequence version replaced NM_053604.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: BC081754.1, U21662.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q24"
     gene            1..2510
                     /gene="Mgat2"
                     /gene_synonym="Gnt2"
                     /note="alpha-1,6-mannosyl-glycoprotein
                     2-beta-N-acetylglucosaminyltransferase"
                     /db_xref="GeneID:94273"
                     /db_xref="RGD:620098"
     exon            1..2490
                     /gene="Mgat2"
                     /gene_synonym="Gnt2"
                     /inference="alignment:Splign:2.1.0"
     CDS             443..1771
                     /gene="Mgat2"
                     /gene_synonym="Gnt2"
                     /EC_number="2.4.1.143"
                     /note="GNT-II; glcNAc-T II;
                     N-glycosyl-oligosaccharide-glycoprotein
                     N-acetylglucosaminyltransferase II;
                     beta-12-N-acetylglucosaminyltransferase II; mannoside
                     acetylglucosaminyltransferase 2;
                     beta-1,2-N-acetylglucosaminyltransferase II; mannosyl
                     (alpha-1,6-)-glycoprotein
                     beta-1,2-N-acetylglucosaminyltransferase"
                     /codon_start=1
                     /product="alpha-1,6-mannosyl-glycoprotein
                     2-beta-N-acetylglucosaminyltransferase precursor"
                     /protein_id="NP_446056.1"
                     /db_xref="GeneID:94273"
                     /db_xref="RGD:620098"
                     /translation="MRFRIYKRKVLILTLVVAACGFVLWSSNGRQRKNDALAPPLLDS
                     EPLRGAGHFAASVGIRRVSNDSAAPLVPAVPRPEVDNLTLRYRSLVYQLNFDQMLRNV
                     DKDGTWSPGELVLVVQVHNRPEYLRLLIDSLRKAQGIREVLVIFSHDFWSAEINSLIS
                     SVDFCPVLQVFFPFSIQLYPSEFPGSDPRDCPRDLKKNAALKLGCINAEYPDSFGHYR
                     EAKFSQTKHHWWWKLHFVWERVKVLQDYTGLILFLEEDHYLAPDFYHVFKKMWKLKQQ
                     ECPGCDVLSLGTYTTIRSFYGIADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFC
                     TYDDYNWDWTLQYLTLACLPKVWKVLVPQAPRIFHAGDCGMHHKKTCRPSTQSAQIES
                     LLNNNKQYLFPETLVIGEKFPMAAISPPRKNGGWGDIRDHELCKSYRRLQ"
ORIGIN      
        1 gtcagaacgg tcgctgccgg agcggggcga ggctgcggcg ttcggtgtcg gcgttccgcc
       61 gtgcgaccaa ggatgagcgc gcagaggacg cagggccacg ggcagcgcag gtaaagaaac
      121 cacggtgtgg cggccgtggc tgggcttctt ccgggacccc gggtgctgaa tggagaggac
      181 cgaggcgaca ctaagccgcg gctcatagcg gcggggcggc tgcttgagct gcaactgcca
      241 gaggatgtgc ggagctcggg cggcgcgagg cagttgaaag ccttcgggcc ccaggacgcc
      301 ggggccgggg atgagttagc gagggccgcc ccgggggcca gttcctaggg ctacaggcca
      361 aggcgacggc gccgcccgcc cgccccttcc gtgcagaggc cgccagctcc tttccgcgcc
      421 cgcgctcccg gccctggaga ccatgaggtt ccgcatctac aaacggaagg tgctaatcct
      481 gacgctcgtg gtggccgcct gcggcttcgt cctctggagt agcaatgggc gacaaaggaa
      541 gaacgacgcc cttgccccgc cgctgctgga ctcggagccc ctacggggtg cgggccattt
      601 cgccgcgtcc gtaggcatcc gcagggtttc taacgactcg gccgctcctc tggttcccgc
      661 ggtcccgcgg ccggaggtgg acaacctaac gctgcggtac cggtccctag tgtaccagtt
      721 gaactttgat cagatgctga ggaatgtcga taaagacggc acctggagtc cgggggagct
      781 ggtgctggtg gtccaagtgc ataacaggcc ggaatacctc aggctgctga tagactcgct
      841 tcgaaaagcc cagggtattc gcgaagtcct agtcatcttt agccatgact tctggtcggc
      901 agagatcaac agtctgatct ctagtgtgga cttctgtccg gttctgcaag tgttctttcc
      961 gttcagcatt cagctgtacc cgagtgagtt tccgggtagt gatcccagag attgccccag
     1021 agacctgaag aagaatgcag ctctcaagtt ggggtgcatc aatgccgaat acccagactc
     1081 cttcggccat tacagagagg ccaaattctc gcaaaccaaa catcactggt ggtggaagct
     1141 gcattttgta tgggaaagag tcaaagttct tcaagattac actggcctta tacttttcct
     1201 ggaagaggac cactacttag ccccagactt ttaccatgtc ttcaaaaaga tgtggaaatt
     1261 gaagcagcag gagtgtcctg ggtgtgacgt cctctctcta gggacctaca ccaccattcg
     1321 gagtttctat ggtattgctg acaaagtaga tgtgaaaact tggaaatcga cagagcacaa
     1381 tatggggcta gccttgaccc gagatgcata tcagaagctt atcgagtgca cggacacttt
     1441 ctgtacttac gatgattata actgggactg gactcttcaa tatttgactc tagcttgtct
     1501 tcctaaagtc tggaaagtct tagttcctca agctcctagg atttttcatg ctggagactg
     1561 tggtatgcat cacaagaaaa catgtaggcc atccacccag agtgcccaaa ttgagtcatt
     1621 attaaataat aataaacagt acctgtttcc agaaactcta gttatcggtg agaagtttcc
     1681 tatggcagcc atttccccac ctaggaaaaa tggagggtgg ggagatatta gggaccatga
     1741 actctgtaaa agttatagaa gactgcagtg agaaaaaaat tcacaattgc aaaagcaaca
     1801 gtgacagtct tctatttttt atatttgtct caatgggata tacaattgaa taaacgatat
     1861 aggaactggt ttctgcttta atacataaac ttcttgtaaa aggtgtccaa atacatagta
     1921 atccttttca gttatgactg attaaaattt caaactgaaa ttttcatttt gagacttggg
     1981 ttttaaaatt cagtctgtat ctgcttaaaa gtaatgatta gattattggt aaaaagaaag
     2041 ggggaaattt gatttaaatc acatctgtta atctttttat ctaaaacttt gtatactttt
     2101 ccactttcaa aagttatttt aagtacagca agatttattt aaaactgtca acagtataaa
     2161 aagtattaca ataaatcatt agagtattgc tgggacagac ccagtttcac tattgacaca
     2221 gtgattagga attgtttgac tggtttaata actcagaaat taagtttgtt aagcaccctg
     2281 ttggaagagt cattttcagt attactgatc cctgtgctat tgtgttaaga tttgcctgtg
     2341 ctttcaacct tcatattaca tgattctttt ggaatgtatt tgattaataa gaaagtttaa
     2401 acaatgtttt cacctcaatg tagaaataca gtggttttga cttttttctt tttagtgctg
     2461 ccaaaataaa atactcattt ttgcataaaa aaaaaaaaaa aaaaaaaaaa
//
