LOCUS       NM_030861               2546 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus alpha-1,3-mannosyl-glycoprotein
            2-beta-N-acetylglucosaminyltransferase (Mgat1), mRNA.
ACCESSION   NM_030861
VERSION     NM_030861.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Chiasserini,D., van Weering,J.R., Piersma,S.R., Pham,T.V.,
            Malekzadeh,A., Teunissen,C.E., de Wit,H. and Jimenez,C.R.
  TITLE     Proteomic analysis of cerebrospinal fluid extracellular vesicles: a
            comprehensive dataset
  JOURNAL   J Proteomics 106, 191-204 (2014)
   PUBMED   24769233
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Hassinen,A., Rivinoja,A., Kauppila,A. and Kellokumpu,S.
  TITLE     Golgi N-glycosyltransferases form both homo- and heterodimeric
            enzyme complexes in live cells
  JOURNAL   J Biol Chem 285 (23), 17771-17777 (2010)
   PUBMED   20378551
REFERENCE   3  (bases 1 to 2546)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   4  (bases 1 to 2546)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   5  (bases 1 to 2546)
  AUTHORS   Fukada,T., Ono,M., Sakata,S., Kioka,N., Sakai,H. and Komano,T.
  TITLE     Genomic structure and 5'-flanking sequences of rat
            N-acetylglucosaminyltransferase I gene and regulatory role of its
            transcriptional diversity
  JOURNAL   Biosci Biotechnol Biochem 67 (7), 1515-1521 (2003)
   PUBMED   12913295
  REMARK    GeneRIF: The unusually long 5'-UTR of the major GnT-I transcript in
            rat brain was shown to inhibit protein production from the
            following coding sequence in COS7 cells
REFERENCE   6  (bases 1 to 2546)
  AUTHORS   Fukada,T., Kioka,N., Nishiu,J., Sakata,S., Sakai,H., Yamada,M. and
            Komano,T.
  TITLE     Different response to inflammation of the multiple mRNAs of rat
            N-acetylglucosaminyltransferase I with variable 5'-untranslated
            sequences
  JOURNAL   FEBS Lett 436 (2), 228-232 (1998)
   PUBMED   9781684
REFERENCE   7  (bases 1 to 2546)
  AUTHORS   Ioffe,E. and Stanley,P.
  TITLE     Mice lacking N-acetylglucosaminyltransferase I activity die at
            mid-gestation, revealing an essential role for complex or hybrid
            N-linked carbohydrates
  JOURNAL   Proc Natl Acad Sci U S A 91 (2), 728-732 (1994)
   PUBMED   8290590
REFERENCE   8  (bases 1 to 2546)
  AUTHORS   Fukada,T., Iida,K., Kioka,N., Sakai,H. and Komano,T.
  TITLE     Cloning of a cDNA encoding N-acetylglucosaminyltransferase I from
            rat liver and analysis of its expression in rat tissues
  JOURNAL   Biosci Biotechnol Biochem 58 (1), 200-201 (1994)
   PUBMED   7764514
REFERENCE   9  (bases 1 to 2546)
  AUTHORS   Kumar,R., Yang,J., Eddy,R.L., Byers,M.G., Shows,T.B. and Stanley,P.
  TITLE     Cloning and expression of the murine gene and chromosomal location
            of the human gene encoding N-acetylglucosaminyltransferase I
  JOURNAL   Glycobiology 2 (4), 383-393 (1992)
   PUBMED   1421759
  REMARK    Erratum:[Glycobiology 1999 Aug;9(8):ix]
REFERENCE   10 (bases 1 to 2546)
  AUTHORS   Kumar,R., Yang,J., Larsen,R.D. and Stanley,P.
  TITLE     Cloning and expression of N-acetylglucosaminyltransferase I, the
            medial Golgi transferase that initiates complex N-linked
            carbohydrate formation
  JOURNAL   Proc Natl Acad Sci U S A 87 (24), 9948-9952 (1990)
   PUBMED   1702225
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from D16302.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D16302.1, EV779263.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q21"
     gene            1..2546
                     /gene="Mgat1"
                     /note="alpha-1,3-mannosyl-glycoprotein
                     2-beta-N-acetylglucosaminyltransferase"
                     /db_xref="GeneID:81519"
                     /db_xref="RGD:620097"
     exon            1..33
                     /gene="Mgat1"
                     /inference="alignment:Splign:2.1.0"
     exon            34..2546
                     /gene="Mgat1"
                     /inference="alignment:Splign:2.1.0"
     CDS             158..1501
                     /gene="Mgat1"
                     /EC_number="2.4.1.101"
                     /note="MGAT1 gene for N-acetylglucosaminyltransferase;
                     GNT-I; glcNAc-T I; N-glycosyl-oligosaccharide-glycoprotein
                     N-acetylglucosaminyltransferase I; mannoside
                     acetylglucosaminyltransferase 1; mannosyl
                     (alpha-1,3-)-glycoprotein
                     beta-1,2-N-acetylglucosaminyltransferase"
                     /codon_start=1
                     /product="alpha-1,3-mannosyl-glycoprotein
                     2-beta-N-acetylglucosaminyltransferase"
                     /protein_id="NP_110488.1"
                     /db_xref="GeneID:81519"
                     /db_xref="RGD:620097"
                     /translation="MLKKQSAGLVLWGAIIFVGWNALLLLFFWTRPAPGRLPSDSALG
                     DDPASLTREVIHLAEDAEAELERQRGLLQQIKEHYSLWRQRWRVPTVAPPAWPRVPGT
                     PSPAVIPILVIACDRSTVRRCLDKLLHYRPSAEHFPIIVSQDCGHEETAQVIASYGTA
                     VTHIRQPDLSNIAVQPDHRKFQGYYKIARHYRWALGQIFNKFKFPAAVVVEDDLEVAP
                     DFFEYFQATYPLLKADPSLWCVSAWNDNGKEQMVDSSKPELLYRTDFFPGLGWLLLAD
                     LWAELEPKWPKAFWDDWMRRPEQRKGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFI
                     KLNQQFVPFTQLDLSYLQREAYDRDFLAQVYGAPQLQVEKVRTNDRKELGEVRVQYTS
                     RDSFKAFAKALGVMDDLKSGVPRAGYRGIVTFQFRGRRVHLAPPETWNGYDPSWN"
ORIGIN      
        1 ctccagggaa gcaacagaga gaagaaaaga aaggtacatg gctacttccc actctcagcc
       61 tagaagaata aacccttaga ttgcggggga actgagccag gcaagccaca ggcagccttg
      121 agcgccccct tgcctgccct cccctgcggg ggccaggatg ctgaagaagc agtctgcagg
      181 gcttgtgctt tggggtgcta tcatctttgt gggctggaat gccctgctgc tcctcttctt
      241 ctggacacgc ccagcacctg gcaggctgcc ctcagacagt gcccttggtg atgaccctgc
      301 cagcctcacc cgtgaggtca tccacctggc cgaggacgcc gaggcggagt tggagcggca
      361 gcggggacta ctgcagcaga tcaaggagca ttattctctg tggaggcaga ggtggagagt
      421 tcccaccgtg gcccctccag cctggccccg tgtgcctggg accccctcac cagctgtgat
      481 ccccattctg gtcattgcct gtgaccgcag cactgtccgg cgctgcctgg ataagttgtt
      541 gcactatcgg ccctctgctg agcatttccc cattattgtc agtcaagact gtgggcatga
      601 agagacagca caggtcattg cttcttatgg caccgctgtc acacacatcc ggcagccaga
      661 cctgagtaac attgccgtgc agccagacca ccgtaagttc cagggttact acaagattgc
      721 caggcactac cgctgggcgc taggccagat cttcaacaag ttcaagttcc cggctgctgt
      781 ggtagtggag gatgatctgg aagtggcgcc ggacttcttc gagtacttcc aggccaccta
      841 cccgctgctg aaagcagacc cctccctttg gtgtgtgtcg gcttggaatg ataatggtaa
      901 ggagcagatg gtggactcca gcaaacctga gctgctctat cgaacagact tttttcctgg
      961 ccttggatgg ctgctgttgg ctgatctctg ggcagaacta gagcccaagt ggcccaaggc
     1021 cttctgggac gactggatgc gcagacctga gcagcggaag gggcgggctt gtattcgtcc
     1081 agaaatttca agaactatga catttggtcg caagggtgtg agccatgggc agttctttga
     1141 ccagcatctt aaattcatca agctgaacca gcagttcgtg cccttcaccc agttggacct
     1201 gtcgtacctg cagcgggagg cctatgaccg ggacttcctt gcccaggtct atggtgcccc
     1261 ccagctgcag gtggagaaag tgaggaccaa tgatcggaag gaactggggg aggtgcgggt
     1321 acagtacact agcagagaca gctttaaggc ctttgctaag gccctgggtg tcatggacga
     1381 cctcaagtct ggtgtcccca gagctggcta ccgtggcatt gtcactttcc agttccgggg
     1441 tcggcgtgtc cacctggcac ccccagagac atggaatggc tatgatccta gctggaatta
     1501 gtggcacctg cctttccctc ctgggtctcc ttgccgtatg atgagccgag gcagcctgca
     1561 ggccctgggc tataccattc tgccagtgtt tcctcttgga tctatagatc ttcccttttt
     1621 gtcctggcct tcccccaagt ggcattctag tgcacaaatc ataagatgag ggttatactc
     1681 ctcttgtcaa gggagtactg tgtggtatgt tcggggcata ttgaacaaga aaccactgtg
     1741 tggtgtgggg agggttggac ctgttggacc aggcttgtag tgtcctgagt tctctccaag
     1801 ggcatctgcg gagagcttgg ccactccagc tctcctgacc aggcctctcc accctgatct
     1861 ggctcctgtt agtacatgag cccactttat gtaccttccc cactttctcc accttcccca
     1921 gggtggggcc ggattatgga agaaggaata gatttgtggc caactgagac taaccaaagg
     1981 gattcactgt cagagttaga ttgcatggcc gggttgccgg ggtcactgcc tcctggcttc
     2041 tcttcctgcc gactttgcca gtgctcttct tgtcctgcag tggagcagtg tgttgtgaga
     2101 tggaaagtag gagctaaagc aagacctctc ccgtggtgga gcagttgtca ggaacaatga
     2161 caggcggaaa gcctacactt gggcacccct cttcttgccc atggcccccc acttctgaaa
     2221 gttagttccc tttgcattct ttagggggat tcagtggcag cctagtgggg gacagtgggc
     2281 tccacgcctc tttcccctgg ggttggtaca gcctcctccc acagggcttg ttcctggtgg
     2341 ccatctcagc aaagtcacca gcagctgaga ccaggacacc tgtggccctt cctaagcatt
     2401 cccacatctt cccaggccga ggggcaggtg gcaggctaga gagaaagaaa tttgtgtgtt
     2461 ttgtttgttg cctgatctta gtttcatgga agaaaatgga atctacagaa ttattttcaa
     2521 aaataaagta aaggctgaat tgtctg
//
