LOCUS       NM_138976               4496 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Rattus norvegicus mitofusin 1 (Mfn1), mRNA.
ACCESSION   NM_138976 XM_039101678
VERSION     NM_138976.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Inagaki,S., Suzuki,Y., Kawasaki,K., Kondo,R., Imaizumi,Y. and
            Yamamura,H.
  TITLE     Mitofusin 1 and 2 differentially regulate mitochondrial function
            underlying Ca2+ signaling and proliferation in rat aortic smooth
            muscle cells
  JOURNAL   Biochem Biophys Res Commun 645, 137-146 (2023)
   PUBMED   36689810
  REMARK    GeneRIF: Mitofusin 1 and 2 differentially regulate mitochondrial
            function underlying Ca[2+] signaling and proliferation in rat
            aortic smooth muscle cells.
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Chen,T., Wang,Y., Wang,Y.H. and Hang,C.H.
  TITLE     The Mfn1-betaIIPKC Interaction Regulates Mitochondrial Dysfunction
            via Sirt3 Following Experimental Subarachnoid Hemorrhage
  JOURNAL   Transl Stroke Res 13 (5), 845-857 (2022)
   PUBMED   35192161
  REMARK    GeneRIF: The Mfn1-betaIIPKC Interaction Regulates Mitochondrial
            Dysfunction via Sirt3 Following Experimental Subarachnoid
            Hemorrhage.
            Erratum:[Transl Stroke Res. 2024 Aug;15(4):861-862. doi:
            10.1007/s12975-024-01253-w.. PMID: 38750251]
REFERENCE   3  (bases 1 to 4496)
  AUTHORS   Mu,G., Deng,Y., Lu,Z., Li,X. and Chen,Y.
  TITLE     miR-20b suppresses mitochondrial dysfunction-mediated apoptosis to
            alleviate hyperoxia-induced acute lung injury by directly targeting
            MFN1 and MFN2
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 53 (2), 220-228 (2021)
   PUBMED   33347533
  REMARK    GeneRIF: miR-20b suppresses mitochondrial dysfunction-mediated
            apoptosis to alleviate hyperoxia-induced acute lung injury by
            directly targeting MFN1 and MFN2.
REFERENCE   4  (bases 1 to 4496)
  AUTHORS   Dai,S.H., Wu,Q.C., Zhu,R.R., Wan,X.M. and Zhou,X.L.
  TITLE     Notch1 protects against myocardial ischaemia-reperfusion injury via
            regulating mitochondrial fusion and function
  JOURNAL   J Cell Mol Med 24 (5), 3183-3191 (2020)
   PUBMED   31975567
  REMARK    GeneRIF: Notch1 protects against myocardial ischaemia-reperfusion
            injury via regulating mitochondrial fusion and function.
REFERENCE   5  (bases 1 to 4496)
  AUTHORS   Yan,L., Qi,Y., Huang,X., Yu,C., Lan,L., Guo,X., Rao,Z., Hu,J. and
            Lou,Z.
  TITLE     Structural basis for GTP hydrolysis and conformational change of
            MFN1 in mediating membrane fusion
  JOURNAL   Nat Struct Mol Biol 25 (3), 233-243 (2018)
   PUBMED   29483649
REFERENCE   6  (bases 1 to 4496)
  AUTHORS   Honda,S. and Hirose,S.
  TITLE     Stage-specific enhanced expression of mitochondrial fusion and
            fission factors during spermatogenesis in rat testis
  JOURNAL   Biochem Biophys Res Commun 311 (2), 424-432 (2003)
   PUBMED   14592431
REFERENCE   7  (bases 1 to 4496)
  AUTHORS   Eura,Y., Ishihara,N., Yokota,S. and Mihara,K.
  TITLE     Two mitofusin proteins, mammalian homologues of FZO, with distinct
            functions are both required for mitochondrial fusion
  JOURNAL   J Biochem 134 (3), 333-344 (2003)
   PUBMED   14561718
  REMARK    GeneRIF: We characterized two rat mitochondrial outer membrane
            proteins, Mfn1 and Mfn2, with distinct tissue expressions, that are
            homologous to Drosophila Fzo, a GTPase involved in mitochondrial
            fusion.
REFERENCE   8  (bases 1 to 4496)
  AUTHORS   Santel,A., Frank,S., Gaume,B., Herrler,M., Youle,R.J. and
            Fuller,M.T.
  TITLE     Mitofusin-1 protein is a generally expressed mediator of
            mitochondrial fusion in mammalian cells
  JOURNAL   J Cell Sci 116 (Pt 13), 2763-2774 (2003)
   PUBMED   12759376
REFERENCE   9  (bases 1 to 4496)
  AUTHORS   Ishihara,N., Jofuku,A., Eura,Y. and Mihara,K.
  TITLE     Regulation of mitochondrial morphology by membrane potential, and
            DRP1-dependent division and FZO1-dependent fusion reaction in
            mammalian cells
  JOURNAL   Biochem Biophys Res Commun 301 (4), 891-898 (2003)
   PUBMED   12589796
REFERENCE   10 (bases 1 to 4496)
  AUTHORS   Chen,H., Detmer,S.A., Ewald,A.J., Griffin,E.E., Fraser,S.E. and
            Chan,D.C.
  TITLE     Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion
            and are essential for embryonic development
  JOURNAL   J Cell Biol 160 (2), 189-200 (2003)
   PUBMED   12527753
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On or before Oct 4, 2022 this sequence version replaced
            XM_039101678.1, NM_138976.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643292.8470.1,
                                           SRR26643287.5303.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-216               JAXUCZ010000002.1  117241758-117241973
            217-245             JAXUCZ010000002.1  117242516-117242544
            246-364             JAXUCZ010000002.1  117244245-117244363
            365-500             JAXUCZ010000002.1  117246802-117246937
            501-663             JAXUCZ010000002.1  117251198-117251360
            664-788             JAXUCZ010000002.1  117255287-117255411
            789-897             JAXUCZ010000002.1  117256603-117256711
            898-1005            JAXUCZ010000002.1  117258711-117258818
            1006-1159           JAXUCZ010000002.1  117262130-117262283
            1160-1227           JAXUCZ010000002.1  117263720-117263787
            1228-1349           JAXUCZ010000002.1  117271866-117271987
            1350-1476           JAXUCZ010000002.1  117273357-117273483
            1477-1581           JAXUCZ010000002.1  117273717-117273821
            1582-1684           JAXUCZ010000002.1  117274152-117274254
            1685-1914           JAXUCZ010000002.1  117274665-117274894
            1915-2067           JAXUCZ010000002.1  117277316-117277468
            2068-2264           JAXUCZ010000002.1  117278505-117278701
            2265-2399           JAXUCZ010000002.1  117283724-117283858
            2400-4496           JAXUCZ010000002.1  117285921-117288017
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q24"
     gene            1..4496
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /note="mitofusin 1"
                     /db_xref="GeneID:192647"
                     /db_xref="RGD:621460"
     exon            1..216
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            217..245
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            246..364
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     CDS             253..2478
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /note="transmembrane GTPase MFN1; mitochondrial
                     transmembrane GTPase FZO1B"
                     /codon_start=1
                     /product="mitofusin-1"
                     /protein_id="NP_620432.2"
                     /db_xref="GeneID:192647"
                     /db_xref="RGD:621460"
                     /translation="MAETVSPLKHFVLAKKAITAIFGQLLEFVTEGSHFVEATYRNPE
                     LDRIATEDDLVEIQGYRNKLAVIGEVLSRRHMKVAFFGRTSSGKSSVINAMLWDKVLP
                     SGIGHTTNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVNQLAHALHMDKDLKAGCLVHV
                     FWPKAKCALLRDDLVLVDSPGTDVTTELDIWIDKFCLDADVFVLVANSESTLMNTEKH
                     FFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVSPLEA
                     RNRIFFVSAKEVLNSRMNKAQGMPEGGGALAEGFQARLQEFQNFEQTFEECISQSAVK
                     TKFEQHTIRAKQILDTVKNILDSVNVAAAEKRVYSMEEREDQIDRLDFIRNQMNLLTM
                     DVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPTPSVLKVYKSELNKHIE
                     DGMGRNLADRCTSEVNASILQSQQEIIENLKPLLPAGIQNKLHTLIPCKKFDLSYDLN
                     CHKLCSDFQEDIVFRFSLGWSSLVHRFLGSTNAQRVLLGLSEPIFQVPRSLASTPTAP
                     SNPAAPDNAAQEELMITLITGLASLTSRTSMGIIVVGGVIWKTVGWKLISVTLSMYGA
                     LYLYERLTWTTRAKERAFKQQFVNYATEKLQMIVKFTSANCSHQVQQEMATTFARLCQ
                     QVDVTQKHLEEEIARLSKEIDQLEKIQNNSKLLRNKAVQLERELENFSKQFLHQSSEE
                     S"
     exon            365..500
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            501..663
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            664..788
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            789..897
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            898..1005
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1159
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1227
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1349
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1476
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1581
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..1684
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1914
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            1915..2067
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2068..2264
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2265..2399
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
     exon            2400..4496
                     /gene="Mfn1"
                     /gene_synonym="Fzo1b"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctcctcccc gctctccggc ggacgagaca gccagggaac tgtggcggag cagtggttgc
       61 tgggtgacag tcgggagctg ggacggaatg agtgaccgct ggcgggtggc gacgcccggg
      121 gtgaccttcg agctccggac aggcgcagcg cgagcgccct gacggcaggg ccgggcaaga
      181 acgaccggcg gccagcctgt gggaaggcct gtccagaact gaagattggg cccctcacat
      241 gctaggtgca taatggcaga aaccgtatct ccactgaagc acttcgtgct ggcaaagaag
      301 gccatcactg cgatcttcgg ccagttactg gagtttgtta ctgagggctc acattttgtt
      361 gaagcaacat acaggaaccc ggaacttgat cggatagcaa ccgaggatga tctggtggag
      421 atacagggct acagaaacaa gctcgctgtc attggggagg tgctgtctcg gaggcatatg
      481 aaagtggcat tttttggcag gacaagtagt ggcaagagct ctgtcatcaa cgcaatgctg
      541 tgggataaag tcctccccag cgggattggt cacacaacca actgcttcct gagtgtggag
      601 ggaactgatg gagataaagc ctaccttatg accgaagggt cagatgagaa gaaaagcgtg
      661 aagactgtta atcagctggc ccatgccctc catatggata aagacttgaa agctggttgt
      721 cttgtgcacg tattttggcc caaagcaaaa tgtgccctct tgagagatga cctggtttta
      781 gtagacagcc caggtacaga tgtcaccaca gagctggaca tctggattga taagttctgc
      841 cttgatgctg atgtttttgt tttggtggca aactcggaat caacgctgat gaacacggag
      901 aaacattttt tccataaggt gaatgagcgg ctctccaagc caaacatttt tatcctgaat
      961 aatcgttggg atgcttctgc ttcagagcca gaatatatgg aggatgtgcg cagacagcac
     1021 atggaaagat gtcttcactt cttggtagaa gagctcaagg ttgtgagtcc tttagaagct
     1081 cggaatcgga tcttttttgt ttcagccaag gaagttctca actccagaat gaataaagcc
     1141 caggggatgc cagaaggtgg aggggcactt gcagaaggat ttcaggcaag attacaggag
     1201 tttcaaaatt ttgaacaaac ttttgaggag tgtatctcgc agtcagcagt gaaaacaaag
     1261 tttgaacagc acactatcag agctaaacag atactagaca ctgtgaaaaa catactggac
     1321 tcagtaaacg tggcagcagc agagaagagg gtttattcaa tggaagagag ggaagaccaa
     1381 atcgacagac tggactttat tcgaaaccag atgaaccttt taacaatgga tgttaagaag
     1441 aagatcaagg aggtcacgga ggaggtggca aacaaggttt cttgtgcaat gacagatgag
     1501 atttgtcgcc tgtctgtttt ggttgatgag ttttgttctg agtttcatcc tacccccagt
     1561 gtgctgaaag tgtataagag tgaattaaat aagcacatag aagatggcat gggaagaaat
     1621 ttggctgatc ggtgcaccag tgaagtcaat gcctccattc ttcaatctca gcaagaaatc
     1681 attgaaaatt tgaagccact acttccagct ggtatacaga ataaacttca tacattaatc
     1741 ccttgcaaaa agtttgatct cagctatgat ctcaattgcc acaagctgtg ttcggatttt
     1801 caagaagaca ttgtgtttcg gttttccctg ggctggtctt cccttgtaca tcgattcctg
     1861 ggttctacga atgcacagag ggtgctgctt gggctgtcag agcctatctt tcaggtccct
     1921 agatctttag cttcaactcc tactgctcct tctaacccag cagcaccaga taatgcagcc
     1981 caggaagagc tcatgatcac actgatcaca ggattggcat ccctcacgtc tagaacctcc
     2041 atgggcatca tagttgttgg gggcgtgatt tggaaaacag tgggctggaa actcatctct
     2101 gtcaccttaa gtatgtatgg agctttgtac ctttatgaaa gactgacttg gactactcgt
     2161 gcgaaagaga gagcgtttaa gcagcagttt gtaaactatg caactgaaaa actgcagatg
     2221 attgtgaagt tcacaagtgc aaactgcagc caccaagtcc agcaagaaat ggccaccact
     2281 tttgctcgac tgtgccaaca agttgatgtt actcagaaac atctggaaga agaaattgct
     2341 agattatcca aagagattga ccaactggag aaaatacaaa acaattcaaa gctcttaaga
     2401 aataaagctg ttcaacttga acgtgagctg gaaaattttt cgaagcagtt tctccaccag
     2461 agcagtgaag aatcctaaca acagaggcac tgtaggagga cgcggacttg gaagatggga
     2521 agtgttactt ttatgagccg acctcagtaa aaatgactga ctctcagtac ctatgccttg
     2581 cggagattgt ggtaatgacc tgtctcaggg cttgcaccgt tggaagtgtt gtgattgact
     2641 tagctttagt ttggagtaaa gactgagttg ttcgggttaa atgatgaatt cctttagaaa
     2701 caatggaacc tgctttgtgg cccctgaggg tgggtcctgc agctcctcac caggctgtct
     2761 gtctgtctgc agctctcagt agctgcttcc tggcatccag gagttagaga ccttttcatc
     2821 ctcttcctca gtgctagttc ttgatgcttc tttaatggga atagtgactt tgtctaaagc
     2881 tgatttgttc aaaccagggg tgtgggctgc acctgggggt cctacagtta ctctgtcctc
     2941 acagcaagga tgtaaccact actgaacagt ttttactttc ttttattccc attaaaactg
     3001 atgtgaaata gtagcaaggc ctgtcattat agccacgcat gaaggaagtg tctgggtctg
     3061 ctgtcctgct ctctctttag aagcatttca gccttgagtc ctgcgaggag aacttctctg
     3121 cctaatgtga agtggacggt ctgaaggggt gaaaatggcc agagggggac aaggctgtgc
     3181 ttgggatgtg gtgacatcac ccaccatgac agtggatcct taggaggact cttactgagg
     3241 cattgggcct gtctccattg gaaactttga attacaagaa tttccttggg ctccattaca
     3301 aattaccact ctgggttagt ggtttaggtt taggttcatg taaatgtctg attgacagtg
     3361 gtatggaaat acttgtgagt agcaggacag gattattctc taccgccacc cccggggaca
     3421 ctgcattcca ctatggtgtc aaaggtcagc atttcgggct tcttgaaagc agagaagtgg
     3481 ttagtatgac tacattgaag agaggggcaa agagatccag caaaccgtcc acctcctttc
     3541 ttcctggagc cttttctcct ttagtcctca gcatttcttt cctacaggtc tgtcctctca
     3601 ataggtacac ctcttgttgg ttggttggtt agtgtgagac agtgtttctc tacgtagtgt
     3661 tggctggcct ggatggaatt tgttctgtag atcaggctgg cctcaattga gagtttcctg
     3721 actctgcctc ctcagtgctg ggattaaagg cgtgcccagc cctagatgca ccttagtttt
     3781 aaaatacttg accatagaga cgtttcagaa ttacttaatt cctatgcaga ttgagtgttc
     3841 agttgcagta ttcaaattta aattcaactg actacctttt gcttttattg ctgattttta
     3901 acacccccca caccccccaa cagtacaggc aaccaagtgg gattggctga aaattacatg
     3961 aaaaactagt atctaaaatt gaggtctgct aacctaggtt acaaagttaa ttagcaagct
     4021 acagcttggt actctgggtt atcaacatca cttttaaccg tgtaagaagt gtggcttatc
     4081 aagcctcaac acaggtatgg aatcaagttg ggtctgcagc aagcttcaag aagccgtgca
     4141 ttgccgccct gtactctgaa tctgtggacc agtgtctgtg gttcagagtg cttttcttcc
     4201 ctggttaaga gcgtggttcc aggtgatgga gggaacacgt cccggtgctg agcctggagt
     4261 gttgacatga gcagctcctt taccaggcaa gagtgacctg tggctcgggg ctcggcatca
     4321 cagactgcag tcatgactac tgcgcattag ttgcaacaca catacttgat gatttactgt
     4381 gtctgtttac aatatttata aactttgtat gatcttaaac ttttacaaaa cattttttaa
     4441 gtatgcaagc ttccaagatg aaaataaacg tatctgccta ttagttgata aatgta
//
