LOCUS       NM_012811               2020 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus milk fat globule EGF and factor V/VIII domain
            containing (Mfge8), transcript variant 2, mRNA.
ACCESSION   NM_012811
VERSION     NM_012811.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Guan,K., Li,H., Chen,H., Qi,X., Wang,R. and Ma,Y.
  TITLE     TMT-based quantitative proteomics analysis reveals the effect of
            bovine derived MFG-E8 against oxidative stress on rat L6 cells
  JOURNAL   Food Funct 12 (16), 7310-7320 (2021)
   PUBMED   34169949
  REMARK    GeneRIF: TMT-based quantitative proteomics analysis reveals the
            effect of bovine derived MFG-E8 against oxidative stress on rat L6
            cells.
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Wang,B., Ge,Z., Wu,Y., Zha,Y., Zhang,X., Yan,Y. and Xie,Y.
  TITLE     MFGE8 is down-regulated in cardiac fibrosis and attenuates
            endothelial-mesenchymal transition through Smad2/3-Snail signalling
            pathway
  JOURNAL   J Cell Mol Med 24 (21), 12799-12812 (2020)
   PUBMED   32945126
  REMARK    GeneRIF: MFGE8 is down-regulated in cardiac fibrosis and attenuates
            endothelial-mesenchymal transition through Smad2/3-Snail signalling
            pathway.
REFERENCE   3  (bases 1 to 2020)
  AUTHORS   Murakami,T., Noguchi,K., Hachiya,N., Kametani,F., Tasaki,M.,
            Nakaba,S., Sassa,Y., Yamashita,T., Obayashi,K., Ando,Y.,
            Hamamura,M., Kanno,T. and Kawasako,K.
  TITLE     Needle-shaped amyloid deposition in rat mammary gland: evidence of
            a novel amyloid fibril protein
  JOURNAL   Amyloid 27 (1), 25-35 (2020)
   PUBMED   31615282
  REMARK    GeneRIF: Needle-shaped amyloid deposition in rat mammary gland:
            evidence of a novel amyloid fibril protein.
REFERENCE   4  (bases 1 to 2020)
  AUTHORS   Ge,Z., Chen,Y., Wang,B., Zhang,X., Yan,Y., Zhou,L., Zhang,Y. and
            Xie,Y.
  TITLE     MFGE8 attenuates Ang-II-induced atrial fibrosis and vulnerability
            to atrial fibrillation through inhibition of TGF-beta1/Smad2/3
            pathway
  JOURNAL   J Mol Cell Cardiol 139, 164-175 (2020)
   PUBMED   31958465
  REMARK    GeneRIF: MFGE8 attenuates Ang-II-induced atrial fibrosis and
            vulnerability to atrial fibrillation through inhibition of
            TGF-beta1/Smad2/3 pathway.
REFERENCE   5  (bases 1 to 2020)
  AUTHORS   Gao,Y.Y., Zhang,Z.H., Zhuang,Z., Lu,Y., Wu,L.Y., Ye,Z.N.,
            Zhang,X.S., Chen,C.L., Li,W. and Hang,C.H.
  TITLE     Recombinant milk fat globule-EGF factor-8 reduces apoptosis via
            integrin beta3/FAK/PI3K/AKT signaling pathway in rats after
            traumatic brain injury
  JOURNAL   Cell Death Dis 9 (9), 845 (2018)
   PUBMED   30154436
  REMARK    GeneRIF: Abolished the effect of rhMFG-E8 on anti-apoptosis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2020)
  AUTHORS   Lee,S.H., Seo,G.S., Park,P.H., Choi,J.Y., Park,Y.N., Kim,H.K.,
            Chae,K.S. and Sohn,D.H.
  TITLE     Increased expression of O-acetyl disialoganglioside synthase during
            rat liver fibrogenesis relates to stellate cell activation
  JOURNAL   Biochem Biophys Res Commun 303 (3), 954-961 (2003)
   PUBMED   12670504
  REMARK    GeneRIF: study shows that OAcGD3S is strongly expressed during
            liver fibrogenesis and hepatic stellate cells seem to be the major
            cellular sources of OAcGD3S in the liver
REFERENCE   7  (bases 1 to 2020)
  AUTHORS   Hanayama,R., Tanaka,M., Miwa,K., Shinohara,A., Iwamatsu,A. and
            Nagata,S.
  TITLE     Identification of a factor that links apoptotic cells to phagocytes
  JOURNAL   Nature 417 (6885), 182-187 (2002)
   PUBMED   12000961
REFERENCE   8  (bases 1 to 2020)
  AUTHORS   Peng,S., Westermark,G.T., Naslund,J., Haggqvist,B., Glennert,J. and
            Westermark,P.
  TITLE     Medin and medin-amyloid in ageing inflamed and non-inflamed
            temporal arteries
  JOURNAL   J Pathol 196 (1), 91-96 (2002)
   PUBMED   11748647
REFERENCE   9  (bases 1 to 2020)
  AUTHORS   Goldberg,G.S., Bechberger,J.F., Tajima,Y., Merritt,M., Omori,Y.,
            Gawinowicz,M.A., Narayanan,R., Tan,Y., Sanai,Y., Yamasaki,H.,
            Naus,C.C., Tsuda,H. and Nicholson,B.J.
  TITLE     Connexin43 suppresses MFG-E8 while inducing contact growth
            inhibition of glioma cells
  JOURNAL   Cancer Res 60 (21), 6018-6026 (2000)
   PUBMED   11085522
REFERENCE   10 (bases 1 to 2020)
  AUTHORS   Ogura,K., Nara,K., Watanabe,Y., Kohno,K., Tai,T. and Sanai,Y.
  TITLE     Cloning and expression of cDNA for O-acetylation of GD3 ganglioside
  JOURNAL   Biochem Biophys Res Commun 225 (3), 932-938 (1996)
   PUBMED   8780713
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            FQ134652.1, BC085754.1 and CB322460.1.
            
            On Sep 7, 2012 this sequence version replaced NM_012811.2.
            
            Transcript Variant: This variant (2) lacks an in-frame coding exon
            compared to variant 1, and encodes a shorter isoform (2) missing an
            internal protein segment compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC085754.1, D84068.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132280, SAMD00132286
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-47                FQ134652.1         1-47
            48-1998             BC085754.1         1-1951
            1999-2020           CB322460.1         1-22                c
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q31"
     gene            1..2020
                     /gene="Mfge8"
                     /gene_synonym="AGS; MFGME8; MFGMP-E8; OAcGD3S"
                     /note="milk fat globule EGF and factor V/VIII domain
                     containing"
                     /db_xref="GeneID:25277"
                     /db_xref="RGD:3083"
     exon            1..154
                     /gene="Mfge8"
                     /gene_synonym="AGS; MFGME8; MFGMP-E8; OAcGD3S"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1365
                     /gene="Mfge8"
                     /gene_synonym="AGS; MFGME8; MFGMP-E8; OAcGD3S"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; O-acetyltransferase milk fat globule membrane
                     protein; lactadherin; MFGM; MFG-E8; SED1;
                     O-acetyltransferase Milk fat glolbule membrane protein;
                     O-acetyl disialoganglioside synthase; O-acetyl GD3
                     ganglioside synthase; milk fat globule-EGF factor 8
                     protein"
                     /codon_start=1
                     /product="lactadherin isoform 2 precursor"
                     /protein_id="NP_036943.1"
                     /db_xref="GeneID:25277"
                     /db_xref="RGD:3083"
                     /translation="MQFSRVLAALCGVLLCASGLFAASGDFCDSSLCLNGGTCLMGQD
                     NDIYCLCPEGFTGLVCNETEKGPCSPNPCFHDAKCLVTEDTQRGDIFTEYICQCPVGY
                     SGIHCELGCSTKLGLEGGAIADSQISASSVYMGFMGLQRWGPELARLYRTGIVNAWTA
                     SSYDSKPWIQVDFLRKMRVSGVMTQGASRAGRAEYLKTFKVAYSLDGRRFEFIQDESG
                     TGDKEFMGNQDNNSLKINMFNPTLEAQYIRLYPVSCHRGCTLRFELLGCELHGCSEPL
                     GLKNNTIPDSQITASSSYKTWNLRAFGWYPHLGRLDNQGKINAWTAQSNSAKEWLQVD
                     LGTQKKVTGIITQGARDFGHIQYVASYKVAHSDDGVQWTVYEEQGTSKVFQGNLDNNS
                     HKKNIFEKPFMARYVRVLPLSWHNRITLRLELLGC"
     exon            155..277
                     /gene="Mfge8"
                     /gene_synonym="AGS; MFGME8; MFGMP-E8; OAcGD3S"
                     /inference="alignment:Splign:2.1.0"
     exon            278..409
                     /gene="Mfge8"
                     /gene_synonym="AGS; MFGME8; MFGMP-E8; OAcGD3S"
                     /inference="alignment:Splign:2.1.0"
     exon            410..591
                     /gene="Mfge8"
                     /gene_synonym="AGS; MFGME8; MFGMP-E8; OAcGD3S"
                     /inference="alignment:Splign:2.1.0"
     exon            592..747
                     /gene="Mfge8"
                     /gene_synonym="AGS; MFGME8; MFGMP-E8; OAcGD3S"
                     /inference="alignment:Splign:2.1.0"
     exon            748..892
                     /gene="Mfge8"
                     /gene_synonym="AGS; MFGME8; MFGMP-E8; OAcGD3S"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1077
                     /gene="Mfge8"
                     /gene_synonym="AGS; MFGME8; MFGMP-E8; OAcGD3S"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1227
                     /gene="Mfge8"
                     /gene_synonym="AGS; MFGME8; MFGMP-E8; OAcGD3S"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..2003
                     /gene="Mfge8"
                     /gene_synonym="AGS; MFGME8; MFGMP-E8; OAcGD3S"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2003
                     /gene="Mfge8"
                     /gene_synonym="AGS; MFGME8; MFGMP-E8; OAcGD3S"
ORIGIN      
        1 attcccctgt gagaggagcg gacgcaggaa ctctccggtc ccagcatcgg agcttgtgga
       61 ccatttcccg cgtcccgcag catgcagttc tcccgtgtgc tggccgcgct gtgcggtgtg
      121 ctgctctgcg cctccggcct cttcgctgcg tccggtgact tctgtgactc cagcctgtgc
      181 ctgaatggtg ggacctgctt gatgggccaa gacaatgaca tctactgcct ctgccctgaa
      241 ggcttcacag gccttgtgtg caacgagact gagaaaggac cgtgttcccc aaacccttgc
      301 ttccacgatg ccaaatgcct ggtgactgag gacacacagc gaggggacat cttcactgag
      361 tacatctgcc agtgccctgt gggctactcg ggcatccact gtgaactcgg ctgttccaca
      421 aagctgggct tggaaggggg cgccattgcc gattcacaga tttctgcctc gtctgtgtat
      481 atgggcttca tgggcttgca gcgctggggc ccggagctgg ctcgcctgta tcgcacaggg
      541 attgtcaatg catggacagc cagcagctat gatagcaagc cctggatcca ggtggacttt
      601 ctgcggaaga tgcgggtatc aggtgtgatg acacagggtg ccagccgtgc cgggagggcg
      661 gaatacctga agaccttcaa ggtggcttac agcctcgatg gacgcaggtt cgagttcatc
      721 caggatgaaa gcggaaccgg agacaaggag tttatgggta accaggacaa caacagcctg
      781 aagattaaca tgttcaaccc cactctggag gcacagtaca taaggctgta ccctgtctcg
      841 tgccaccgcg gctgcaccct ccgcttcgag ctcctgggct gcgagttgca tggatgctct
      901 gagcccctgg gcctgaagaa taacacgatt cctgacagcc aaataacagc ctccagcagc
      961 tacaagacgt ggaacctgcg tgcctttggc tggtaccccc acttggggcg gctggacaat
     1021 cagggcaaga tcaatgcctg gacagctcag agcaacagtg ccaaggaatg gctgcaggtt
     1081 gacctgggca ctcagaaaaa agtgacagga attatcaccc agggggcccg tgactttggc
     1141 cacatccagt atgtggcatc ctataaggta gcccacagtg atgatggtgt gcagtggacc
     1201 gtatatgagg aacaaggaac cagcaaggtc ttccagggca acttggacaa caactcccac
     1261 aagaagaaca tctttgagaa acctttcatg gctcgctatg tgcgtgtcct tccactgtcc
     1321 tggcataacc gtatcaccct gcgcctggag ctgctgggct gttagtgccc agtccttcca
     1381 gcccaagtga cgaggacggc cagaggctga ggggcctcct ggccctgcct cccaggccct
     1441 gctgccttct gtggctgaca ccttctcaat cctccctctt gattgcactg ggactacagg
     1501 caggaagggc aagggggttt cagagttgcc cctcaccctt cccctcaccc tgcagccccc
     1561 acaggcctcc tgctagcccc ttctctcagg cattctgggg gagttggaca ggtctgagat
     1621 gaatagagaa gaagagtgaa gttggggtat gtgggctgct gtaccaacca ccccaagtcc
     1681 taaactttct ccaggggttg actcaagact aaagggaacc tctggttgcc cacccgtctc
     1741 tgcacaccgc acatccctcc atgttccatt cctggaagga gaggcccacg tccgcttgct
     1801 gccccttggg tcaccagatc ctgcctctta tctcctgaga cccctcttga ccctcgctct
     1861 ggagcctcgg ttgacaagag gactgtcggg tctggagaga tagatgggct ctgggtggtt
     1921 ggcgagctgg ctatgggacc tctgctggct tgctacccaa gctaacaagc agattccaaa
     1981 atacatttgt gctctccact ggaaaaaaaa aaaaaaaaaa
//
