LOCUS       NM_001399513            2321 bp    mRNA    linear   ROD 22-SEP-2024
DEFINITION  Rattus norvegicus methyltransferase 8, tRNA N3-cytidine (Mettl8),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001399513
VERSION     NM_001399513.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Xu,L., Liu,X., Sheng,N., Oo,K.S., Liang,J., Chionh,Y.H., Xu,J.,
            Ye,F., Gao,Y.G., Dedon,P.C. and Fu,X.Y.
  TITLE     Three distinct 3-methylcytidine (m3C) methyltransferases modify
            tRNA and mRNA in mice and humans
  JOURNAL   J Biol Chem 292 (35), 14695-14703 (2017)
   PUBMED   28655767
REFERENCE   3  (bases 1 to 2321)
  AUTHORS   Jakkaraju,S., Zhe,X., Pan,D., Choudhury,R. and Schuger,L.
  TITLE     TIPs are tension-responsive proteins involved in myogenic versus
            adipogenic differentiation
  JOURNAL   Dev Cell 9 (1), 39-49 (2005)
   PUBMED   15992539
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR21887622.2483128.1,
                                           SRR26643298.16273.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-218               JAXUCZ010000003.1  76271146-76271363   c
            219-373             JAXUCZ010000003.1  76232651-76232805   c
            374-465             JAXUCZ010000003.1  76209481-76209572   c
            466-806             JAXUCZ010000003.1  76196112-76196452   c
            807-856             JAXUCZ010000003.1  76194461-76194510   c
            857-920             JAXUCZ010000003.1  76190143-76190206   c
            921-1060            JAXUCZ010000003.1  76187138-76187277   c
            1061-1167           JAXUCZ010000003.1  76180347-76180453   c
            1168-1233           JAXUCZ010000003.1  76179660-76179725   c
            1234-2321           JAXUCZ010000003.1  76177904-76178991   c
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q22"
     gene            1..2321
                     /gene="Mettl8"
                     /gene_synonym="AABR07052486.1; RGD1561059"
                     /note="methyltransferase 8, tRNA N3-cytidine"
                     /db_xref="GeneID:502633"
                     /db_xref="RGD:1561059"
     exon            1..218
                     /gene="Mettl8"
                     /gene_synonym="AABR07052486.1; RGD1561059"
                     /inference="alignment:Splign:2.1.0"
     exon            219..373
                     /gene="Mettl8"
                     /gene_synonym="AABR07052486.1; RGD1561059"
                     /inference="alignment:Splign:2.1.0"
     CDS             231..1421
                     /gene="Mettl8"
                     /gene_synonym="AABR07052486.1; RGD1561059"
                     /note="isoform 1 is encoded by transcript variant 1;
                     methyltransferase-like protein 8; mRNA N(3)-methylcytidine
                     methyltransferase METTL8; methyltransferase like 8;
                     methyltransferase 8, methylcytidine; tRNA
                     N(3)-methylcytidine methyltransferase METTL8,
                     mitochondrial"
                     /codon_start=1
                     /product="tRNA N(3)-methylcytidine methyltransferase
                     METTL8, mitochondrial isoform 1"
                     /protein_id="NP_001386442.1"
                     /db_xref="GeneID:502633"
                     /db_xref="RGD:1561059"
                     /translation="MNVIWRNCICRLRQGKVPHRCQSGVHPVAPLGSRILTDPTKVFE
                     HNMWDHMQWSKEEEDEARKKVEENSATRVAPEEQVKFENAANKYWDTFYQTHKNKFFK
                     NRNWLLREFPEILPVDQNTKEKLGESSWDPARSSISRTQGTETHRQETFVSSEPGSRE
                     RSASNPDLEEYSRGPRKAEQFPGSKATFRILEVGCGAGNSVFPILNTLQNIPGSFLYC
                     CDFAPEAVELVKSHEAYSEAHCSAFIHDVCDDGLAYPFPDGTLDVILLVFVLSSIHPD
                     RMQAVIHRLSRLLKPGGMLLFRDHGRYDNAQLRFKKGRCLSENFYVRGDGTRAYFFTK
                     GEIHRMFCEAGLHEKQNLVDHRLQVNRKKQIQMHRVWVQGKFQKPSCWTPRSADEHAL
                     PSPD"
     exon            374..465
                     /gene="Mettl8"
                     /gene_synonym="AABR07052486.1; RGD1561059"
                     /inference="alignment:Splign:2.1.0"
     exon            466..806
                     /gene="Mettl8"
                     /gene_synonym="AABR07052486.1; RGD1561059"
                     /inference="alignment:Splign:2.1.0"
     exon            807..856
                     /gene="Mettl8"
                     /gene_synonym="AABR07052486.1; RGD1561059"
                     /inference="alignment:Splign:2.1.0"
     exon            857..920
                     /gene="Mettl8"
                     /gene_synonym="AABR07052486.1; RGD1561059"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1060
                     /gene="Mettl8"
                     /gene_synonym="AABR07052486.1; RGD1561059"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1167
                     /gene="Mettl8"
                     /gene_synonym="AABR07052486.1; RGD1561059"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1233
                     /gene="Mettl8"
                     /gene_synonym="AABR07052486.1; RGD1561059"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..2321
                     /gene="Mettl8"
                     /gene_synonym="AABR07052486.1; RGD1561059"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtgggtaag gtagtcttcc gggctgtacg cggctaagtt ggagagactt gggggcgctg
       61 tgcagccgct tggtggtgta gcttctcccg tgtgtcctgc accaaagaag cagtcccttt
      121 ctgccctggt ggtgggacgc ttgccaaccc cacctttact ctacaaggct ctggccacgt
      181 gtccagaggg gcgttgtgtc cagtgggacc ctccaaaggt cctgtttggg atgaatgtga
      241 tttggagaaa ttgcatttgc cgtctgaggc aagggaaggt gccacacaga tgtcagagcg
      301 gtgtccaccc ggtggcccct ctggggtcaa ggattttaac tgaccccacc aaagttttcg
      361 aacacaacat gtgggatcac atgcagtggt ccaaagagga agaagacgaa gctagaaaaa
      421 aagtagagga aaactcagcc acacgagtcg ccccagaaga gcaagttaag tttgaaaatg
      481 ctgctaacaa atactgggac actttttacc aaacccataa gaataagttt ttcaagaatc
      541 gtaattggct gttgagggaa tttcctgaaa ttcttcccgt tgatcagaac actaaagaga
      601 aactgggaga atcatcctgg gacccagcca gaagcagcat ctcaagaacg caaggaacag
      661 aaacccaccg acaagagact tttgtctcat cagaacctgg gagcagagaa aggtctgctt
      721 ctaaccctga cttggaagaa tacagcagag gacccaggaa ggcagagcag tttcctggta
      781 gcaaggctac ttttcgaata ctagaggttg gctgtggtgc tggaaatagc gtgtttccaa
      841 tcctgaacac cttgcagaac atcccaggat cctttcttta ctgctgtgac ttcgcccctg
      901 aagcagtgga gctcgtaaag tcacatgagg cctacagcga ggcccactgt tctgccttta
      961 ttcatgacgt gtgtgacgac ggcttggcct acccttttcc agacgggacc ctggacgtca
     1021 ttctccttgt ctttgtgctc tcttctattc accctgacag gatgcaagct gtcatccacc
     1081 ggctgtccag gttgctgaag cccggaggaa tgctattgtt tcgggatcat ggaagatacg
     1141 acaatgctca gcttcgcttt aaaaaagggc gttgtttatc tgaaaatttt tacgtccgag
     1201 gggatggtac cagagcctat ttctttacaa aaggggagat ccaccgtatg ttctgcgagg
     1261 ccggattaca tgaaaagcaa aatctggttg atcatcgctt gcaagttaat aggaaaaagc
     1321 aaatacaaat gcaccgagtg tgggttcaag ggaaattcca gaaaccatcg tgctggactc
     1381 cacggagtgc agatgagcac gctctccctt ctccagactg aactgttaaa gtaggaaacc
     1441 agggcatggt accgttcaaa ggcttacgag caggagagca tctgcagctc tgctggcctg
     1501 ctatcagtgc tcttgggaca ttttaagaac acagacgtga cttgaaagag tgtcctaccc
     1561 tctgggtttt cagaacttga aaaatgtttg gttttgtttc atctaaccac gttcccttga
     1621 atggtatgac tttgaattaa gtagctgata taaggcatat agttttgcaa cccatccatg
     1681 ttttagaagt ggaaatatta tctactttat tattgggtgt ggtaaagcag tcagactgtc
     1741 ctcagctgcc agccagtagc agaggcacag tgtgccttgt tcttagggga tgggatgttc
     1801 ttaggatgaa gggatgctgt gttttgtttt gttctcatct attttctctt aaccccttac
     1861 ccccacacac tagtgtctga aatgcaactt ctgtttccct tcctttgcaa ataatgggga
     1921 agtaaggcct tgtcaccacc cctccagggc tgttctcagc tacacagatc atctgagaga
     1981 cgctgtcatg ccgtggtggc tagtttcatt tgtcaacttg acacactcta gaatcactgt
     2041 ggaagagtct caggttgtca gttgtctgtg aaagtttgtc ttaattgtgt taatcgatgt
     2101 gggaagacct gtctactggg ggcagtacca ttccctgggg tttggaccct gaaccaagtg
     2161 tgggaaaagt gagctgagcc ctgacccact tgcattcttc gttctcactc tgctcttgac
     2221 tgctcttgac cagctgcttc aagttcctgc ctccctgact tactgctgca actgggtgga
     2281 ctgtaacttg gagttgtgat ccgaaataaa ccttttctgc c
//
