LOCUS       NM_013143               2928 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Rattus norvegicus meprin A subunit alpha (Mep1a), mRNA.
ACCESSION   NM_013143 XM_001068729
VERSION     NM_013143.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Bertenshaw,G.P., Norcum,M.T. and Bond,J.S.
  TITLE     Structure of homo- and hetero-oligomeric meprin metalloproteases.
            Dimers, tetramers, and high molecular mass multimers
  JOURNAL   J Biol Chem 278 (4), 2522-2532 (2003)
   PUBMED   12399461
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Bertenshaw,G.P., Villa,J.P., Hengst,J.A. and Bond,J.S.
  TITLE     Probing the active sites and mechanisms of rat metalloproteases
            meprin A and B
  JOURNAL   Biol Chem 383 (7-8), 1175-1183 (2002)
   PUBMED   12437103
  REMARK    GeneRIF: optimization of activity by pH and NaCl concentration and
            relevance to catalytic mechanism
REFERENCE   3  (bases 1 to 2928)
  AUTHORS   van Niel,G., Raposo,G., Candalh,C., Boussac,M., Hershberg,R.,
            Cerf-Bensussan,N. and Heyman,M.
  TITLE     Intestinal epithelial cells secrete exosome-like vesicles
  JOURNAL   Gastroenterology 121 (2), 337-349 (2001)
   PUBMED   11487543
REFERENCE   4  (bases 1 to 2928)
  AUTHORS   Eldering,J.A., Grunberg,J., Hahn,D., Croes,H.J., Fransen,J.A. and
            Sterchi,E.E.
  TITLE     Polarised expression of human intestinal
            N-benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase (human meprin)
            alpha and beta subunits in Madin-Darby canine kidney cells
  JOURNAL   Eur J Biochem 247 (3), 920-932 (1997)
   PUBMED   9288916
REFERENCE   5  (bases 1 to 2928)
  AUTHORS   Chevallier,S., Ahn,J., Boileau,G. and Crine,P.
  TITLE     Identification of the cysteine residues implicated in the formation
            of alpha 2 and alpha/beta dimers of rat meprin
  JOURNAL   Biochem J 317 (Pt 3) (Pt 3), 731-738 (1996)
   PUBMED   8760356
REFERENCE   6  (bases 1 to 2928)
  AUTHORS   Gorbea,C.M., Marchand,P., Jiang,W., Copeland,N.G., Gilbert,D.J.,
            Jenkins,N.A. and Bond,J.S.
  TITLE     Cloning, expression, and chromosomal localization of the mouse
            meprin beta subunit
  JOURNAL   J Biol Chem 268 (28), 21035-21043 (1993)
   PUBMED   8407940
REFERENCE   7  (bases 1 to 2928)
  AUTHORS   Jiang,W., Sadler,P.M., Jenkins,N.A., Gilbert,D.J., Copeland,N.G.
            and Bond,J.S.
  TITLE     Tissue-specific expression and chromosomal localization of the
            alpha subunit of mouse meprin A
  JOURNAL   J Biol Chem 268 (14), 10380-10385 (1993)
   PUBMED   7683677
REFERENCE   8  (bases 1 to 2928)
  AUTHORS   Corbeil,D., Gaudoux,F., Wainwright,S., Ingram,J., Kenny,A.J.,
            Boileau,G. and Crine,P.
  TITLE     Molecular cloning of the alpha-subunit of rat endopeptidase-24.18
            (endopeptidase-2) and co-localization with endopeptidase-24.11 in
            rat kidney by in situ hybridization
  JOURNAL   FEBS Lett 309 (2), 203-208 (1992)
   PUBMED   1505684
REFERENCE   9  (bases 1 to 2928)
  AUTHORS   Johnson,G.D. and Hersh,L.B.
  TITLE     Cloning a rat meprin cDNA reveals the enzyme is a heterodimer
  JOURNAL   J Biol Chem 267 (19), 13505-13512 (1992)
   PUBMED   1377685
  REMARK    Erratum:[J Biol Chem 1993 Aug 15;268(23):17647]
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from S43408.1.
            
            On Jun 25, 2006 this sequence version replaced XM_001068729.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081834.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q13"
     gene            1..2928
                     /gene="Mep1a"
                     /note="meprin A subunit alpha"
                     /db_xref="GeneID:25684"
                     /db_xref="RGD:3080"
     exon            1..98
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
     CDS             33..2279
                     /gene="Mep1a"
                     /EC_number="3.4.24.18"
                     /note="meprin A alpha; meprin 1 alpha; MEP-1; E-24.18;
                     endopeptidase-2; endopeptidase-24.18 subunit alpha; meprin
                     1 subunit alpha"
                     /codon_start=1
                     /product="meprin A subunit alpha precursor"
                     /protein_id="NP_037275.1"
                     /db_xref="GeneID:25684"
                     /db_xref="RGD:3080"
                     /translation="MLWTLPVCLLSLSFSAHIAAVSIQHLSTGHDHDDVDVGEQQKDI
                     SEINSAAGLNLFQGDILLPRTRNALRDPSSRWKPPIPYILADNLDLNAKGAILNAFEM
                     FRLKSCVDFKPYEGESSYIIFQQFSGCWSMVGDQHVGQNISIGEGCDYKAIIEHEILH
                     ALGFFHEQSRTDRDDYVNIWWNEIMTDYEHNFNTYDDKTITDLNTPYDYESLMHYGPF
                     SFNKNETIPTITTKIPEFNAIIGQRLDFSATDLTRLNRMYNCTRTHTLLDHCAFEKTN
                     ICGMIQGTRDDADWVHEDSSQPGQVDHTLVGRCKAAGYFMYFNTSSGVTGEVALLESR
                     ILYPKRKQQCLQFFYKMTGSPSDRLLIWVRRDDNTGNVRQLAKIQTFQGDSDHNWKIA
                     HVTLNEEKKFRYVFQGTKGDPGNSDGGIYLDDITLTETPCPTGVWTIRNISQVLENTV
                     KGDRLVSPRFYNSEGYGFGVTLYPNGRITSNSGYLGLAFHLYSGDNDVILEWPVENEQ
                     AIMTILDQEPDARNRMSLSLMFTTSKYQTSSAINGSVIWDRPTKVGVYDKDCDCFRSI
                     DWGWGQAISHQMLMRRNFLKDDTLIIFVDFKDLTHLRQTEVPISSRSVIPRGLLLQGQ
                     EPLALGDSRIAMMEESLPRRLDQRQPSRPKRSVENTGPMEDHNWPQYFRDPCDPNPCQ
                     NEGTCVNVKGMASCRCVSGHAFFYTGERCQAMHVHGSLLGLLIGCITALIFLTFITFS
                     NTYQKLRQ"
     exon            99..123
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
     exon            124..180
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
     exon            181..221
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
     exon            222..297
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
     exon            298..415
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
     exon            416..591
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
     exon            592..813
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
     exon            814..963
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1179
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1650
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1824
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..2122
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..2919
                     /gene="Mep1a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaggttctgc caaacgcagt tcacttgcaa caatgctgtg gacactacct gtgtgtcttc
       61 tctcactgag tttttctgcc cacattgcag ctgtgtccat tcagcatctt tcgactggac
      121 atgaccacga tgatgtagac gttggtgaac aacagaagga catttcagaa atcaactcag
      181 ctgcaggcct gaaccttttt caaggggaca tccttctgcc cagaaccaga aatgccctga
      241 gagatccgtc aagcagatgg aagcccccca ttccatacat tctggctgac aatctggatc
      301 tgaacgccaa aggagccatc ctcaatgcct ttgagatgtt ccgcctcaag tcttgtgtgg
      361 acttcaagcc ctatgagggg gagagctcgt acatcatctt ccagcagttt tctgggtgct
      421 ggtctatggt cggtgatcaa cacgtgggac agaatatttc cattggtgag ggctgcgact
      481 ataaggccat cattgaacat gagatcctgc atgctctggg attcttccat gagcagtcaa
      541 ggactgaccg ggatgattat gtgaacatct ggtggaatga aattatgaca gattatgaac
      601 ataatttcaa cacgtatgat gacaagacca tcacagacct caacacaccc tatgattatg
      661 agtccttgat gcactatggg ccattttcat ttaacaagaa tgaaaccatc ccgaccatca
      721 ctaccaagat ccctgagttc aacgccatca ttggacagcg cctggatttc agtgccactg
      781 atttaacgag actgaatcgg atgtacaact gcaccagaac acataccctg ctggaccact
      841 gtgcgtttga gaagaccaac atctgtggaa tgatccaggg gaccagagat gatgctgact
      901 gggttcatga ggacagcagt cagcctggac aagtggatca caccttggtg ggacggtgca
      961 aagctgctgg ctacttcatg tacttcaata ccagctcggg ggtgaccgga gaggtagccc
     1021 ttctggagtc tcggatcctt taccccaaga ggaagcagca gtgtttgcag tttttttata
     1081 agatgacagg aagcccttca gacagactcc tcatctgggt gagaagggac gacaacacgg
     1141 gcaacgtgcg ccagctggcc aagatccaga cttttcaagg agattctgat cacaactgga
     1201 aaattgctca cgtgacactc aacgaagaga agaaatttcg ctatgttttc cagggtacca
     1261 aaggtgaccc tggtaactca gatggcggta tttaccttga tgacatcact ctgacagaaa
     1321 ccccctgccc tacaggggtt tggaccatcc ggaatatctc ccaggtcctt gagaacacag
     1381 tgaaagggga caggctcgtg agccctaggt tctacaattc cgagggttat ggttttgggg
     1441 tgaccttgta ccccaatggt agaatcacca gtaactctgg ctacctggga ctcgctttcc
     1501 acttgtacag tggggataac gatgtgattc tggagtggcc agtggagaac gagcaggcca
     1561 taatgaccat cctggaccag gaacctgatg ccaggaacag gatgtccttg agtttgatgt
     1621 tcactacctc caagtaccaa acatcttcag ctataaatgg ctctgtcatc tgggacaggc
     1681 caaccaaggt tggagtctat gataaggact gtgattgttt tagaagcatt gactggggtt
     1741 ggggtcaggc catttcccac cagatgctga tgagaagaaa tttccttaaa gatgacaccc
     1801 tcatcatctt cgtggacttt aaagacctta cccacttgag gcagactgaa gtccccattt
     1861 ccagcagaag cgtgataccc cgaggactcc ttctgcaagg ccaggagcct cttgccttgg
     1921 gagactccag aatagccatg atggaggaat ccctgccaag aaggctggac cagagacagc
     1981 ccagcagacc aaagcgctca gtggaaaata ctggtcccat ggaggaccac aactggccac
     2041 agtacttcag agacccatgt gacccaaacc cttgccagaa tgaaggcacc tgtgtgaacg
     2101 taaagggaat ggccagctgc aggtgcgtct ctgggcatgc ctttttctac acaggggagc
     2161 gctgtcaggc tatgcatgtg cacggaagct tgctgggcct gctcattggc tgcatcactg
     2221 ctttgatctt cttgaccttc atcacattct ccaacactta ccaaaagctg aggcagtgag
     2281 ctgatgtcca ctagactgca gaagggcctc cttcacctag actttccttc cagtagtcag
     2341 tataatttgg ggatattctt catcaaacat gtttgaaaga gcctccaaaa ccaaagcccc
     2401 aaacctcatg tggggctctt gccactccat ttctgtacag ttctgctatc cttctatgag
     2461 tttctggtga atccattatg tcctaagata acagtcattg cattttacta taaacacttt
     2521 gcttgcttga ccttctcccc ttaaatcacc aagtctagtt gggtaggagc atgttatcct
     2581 ctgctaccac attgacccca gtgctagaag cataccttac tggtgtattg tgggtactca
     2641 gtaagcatta aatgaatgaa ccaataagca agtgaatagc tgtggtataa taagtctttt
     2701 tttcttcctc atattgtaaa acatatgatt aagtctattt gcaggtgtat cagaaccaaa
     2761 tacccagttt acactagatg ggaaaactac tgtcttattg ctcatgtagt ttcttgtgaa
     2821 atgatttcac agattcctct ggtcagctga gtttccatgg catgtacttt tttcttgttc
     2881 tgattttaag gaaaaggctt aataaaacca ttaaatatga aaaaaaaa
//
