LOCUS       NM_001395606            2463 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus maternal embryonic leucine zipper kinase (Melk),
            mRNA.
ACCESSION   NM_001395606 XM_006238082
VERSION     NM_001395606.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Moravcevic,K., Mendrola,J.M., Schmitz,K.R., Wang,Y.H.,
            Slochower,D., Janmey,P.A. and Lemmon,M.A.
  TITLE     Kinase associated-1 domains drive MARK/PAR1 kinases to membrane
            targets by binding acidic phospholipids
  JOURNAL   Cell 143 (6), 966-977 (2010)
   PUBMED   21145462
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   3  (bases 1 to 2463)
  AUTHORS   Jung,H., Seong,H.A. and Ha,H.
  TITLE     Murine protein serine/threonine kinase 38 activates apoptosis
            signal-regulating kinase 1 via Thr 838 phosphorylation
  JOURNAL   J Biol Chem 283 (50), 34541-34553 (2008)
   PUBMED   18948261
REFERENCE   4  (bases 1 to 2463)
  AUTHORS   Lin,M.L., Park,J.H., Nishidate,T., Nakamura,Y. and Katagiri,T.
  TITLE     Involvement of maternal embryonic leucine zipper kinase (MELK) in
            mammary carcinogenesis through interaction with Bcl-G, a
            pro-apoptotic member of the Bcl-2 family
  JOURNAL   Breast Cancer Res 9 (1), R17 (2007)
   PUBMED   17280616
REFERENCE   5  (bases 1 to 2463)
  AUTHORS   Beullens,M., Vancauwenbergh,S., Morrice,N., Derua,R., Ceulemans,H.,
            Waelkens,E. and Bollen,M.
  TITLE     Substrate specificity and activity regulation of protein kinase
            MELK
  JOURNAL   J Biol Chem 280 (48), 40003-40011 (2005)
   PUBMED   16216881
REFERENCE   6  (bases 1 to 2463)
  AUTHORS   Nakano,I., Paucar,A.A., Bajpai,R., Dougherty,J.D., Zewail,A.,
            Kelly,T.K., Kim,K.J., Ou,J., Groszer,M., Imura,T., Freije,W.A.,
            Nelson,S.F., Sofroniew,M.V., Wu,H., Liu,X., Terskikh,A.V.,
            Geschwind,D.H. and Kornblum,H.I.
  TITLE     Maternal embryonic leucine zipper kinase (MELK) regulates
            multipotent neural progenitor proliferation
  JOURNAL   J Cell Biol 170 (3), 413-427 (2005)
   PUBMED   16061694
REFERENCE   7  (bases 1 to 2463)
  AUTHORS   Mirey,G., Chartrain,I., Froment,C., Quaranta,M., Bouche,J.P.,
            Monsarrat,B., Tassan,J.P. and Ducommun,B.
  TITLE     CDC25B phosphorylated by pEg3 localizes to the centrosome and the
            spindle poles at mitosis
  JOURNAL   Cell Cycle 4 (6), 806-811 (2005)
   PUBMED   15908796
REFERENCE   8  (bases 1 to 2463)
  AUTHORS   Davezac,N., Baldin,V., Blot,J., Ducommun,B. and Tassan,J.P.
  TITLE     Human pEg3 kinase associates with and phosphorylates CDC25B
            phosphatase: a potential role for pEg3 in cell cycle regulation
  JOURNAL   Oncogene 21 (50), 7630-7641 (2002)
   PUBMED   12400006
REFERENCE   9  (bases 1 to 2463)
  AUTHORS   Heyer,B.S., Kochanowski,H. and Solter,D.
  TITLE     Expression of Melk, a new protein kinase, during early mouse
            development
  JOURNAL   Dev Dyn 215 (4), 344-351 (1999)
   PUBMED   10417823
REFERENCE   10 (bases 1 to 2463)
  AUTHORS   Gil,M., Yang,Y., Lee,Y., Choi,I. and Ha,H.
  TITLE     Cloning and expression of a cDNA encoding a novel protein
            serine/threonine kinase predominantly expressed in hematopoietic
            cells
  JOURNAL   Gene 195 (2), 295-301 (1997)
   PUBMED   9305775
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Jul 21, 2021 this sequence version replaced XM_006238082.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643294.21043.1,
                                           SRR26643292.21512.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-87                JAXUCZ010000005.1  63336151-63336237
            88-183              JAXUCZ010000005.1  63340447-63340542
            184-269             JAXUCZ010000005.1  63342283-63342368
            270-386             JAXUCZ010000005.1  63344558-63344674
            387-530             JAXUCZ010000005.1  63346576-63346719
            531-599             JAXUCZ010000005.1  63348015-63348083
            600-692             JAXUCZ010000005.1  63350237-63350329
            693-791             JAXUCZ010000005.1  63356189-63356287
            792-860             JAXUCZ010000005.1  63363070-63363138
            861-959             JAXUCZ010000005.1  63364475-63364573
            960-1046            JAXUCZ010000005.1  63371518-63371604
            1047-1166           JAXUCZ010000005.1  63379032-63379151
            1167-1277           JAXUCZ010000005.1  63383913-63384023
            1278-1509           JAXUCZ010000005.1  63385622-63385853
            1510-1606           JAXUCZ010000005.1  63388551-63388647
            1607-1775           JAXUCZ010000005.1  63389783-63389951
            1776-1879           JAXUCZ010000005.1  63392807-63392910
            1880-2463           JAXUCZ010000005.1  63395671-63396254
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q22"
     gene            1..2463
                     /gene="Melk"
                     /note="maternal embryonic leucine zipper kinase"
                     /db_xref="GeneID:362510"
                     /db_xref="RGD:1308974"
     exon            1..87
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            88..183
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..2057
                     /gene="Melk"
                     /EC_number="2.7.11.1"
                     /codon_start=1
                     /product="maternal embryonic leucine zipper kinase"
                     /protein_id="NP_001382535.1"
                     /db_xref="GeneID:362510"
                     /db_xref="RGD:1308974"
                     /translation="MKDYDELLKYYELYETIGTGGFAKVKLACHILTGEMVAIKIMDK
                     NALGSDLPRVKTEIDALKNLRHQHICQLYHVLETKNKIFMVLEYCPGGELFDYIISQD
                     RLSEEETRVIFRQILSAVAYVHSQGYAHRDLKPENLLFDENHKLKLIDFGLCAKPKGN
                     KDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALY
                     KKIMRGKYEVPKWLSPSSILLLQQMLQVDPKRRISMKNLLNHPWILQDYSCPIEWQSK
                     ASLTHLDEDCITELSVHHRNSRQTMEDLISSWQYDHLTATYLLLLAKKARGKPARLQL
                     LSFSGGTTSTTPKSKNLSLEDMSTSGDSCVAGLIDYELCEDNLLAPQTPQFTKHWAES
                     NHVECESPTPVVCRALANKLKDKENVCTPKSSVKNQEQFVFSEPKIPVSRNQYKREIL
                     TSPTQFTAPAKARTQCLRETQVRTPVNSAGADTLTTNVISPERRCRSMEVDLNQAHME
                     DTPKRKGTNVFGSLERGLDKVLTALTRNKKKGSAKDGPRRRKLHYNVTTTRLVNPDQL
                     LNEIMAILPKKNVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQRPDVVGIRRQRLK
                     GDAWVYKRLVEDILSGCKM"
     exon            184..269
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            270..386
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            387..530
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            531..599
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            600..692
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            693..791
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            792..860
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            861..959
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1046
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1166
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1277
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1509
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1606
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1775
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1879
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..2463
                     /gene="Melk"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 caccgactgt tccggctttg ctaggctctg ctgccggctg cttcaccctc tgtcctgttc
       61 ggtcctgcca ggcccgctgc cctttaggtt cttgttgtga ttttgaataa gcttccagct
      121 ggactatgaa agattatgac gagctcctta aatactatga attatatgaa actattggga
      181 caggtggctt tgcaaaggtc aaacttgcct gtcatatcct cactggagaa atggtagcta
      241 taaaaatcat ggataagaat gcactaggga gcgatttgcc ccgagtcaaa actgaaattg
      301 atgccctgaa gaatctgaga catcagcata tatgtcagct ctaccatgtg ctagagacaa
      361 agaacaaaat attcatggtg cttgagtact gtccaggagg agagctgttt gactacatca
      421 tctcccagga tcgcctgtcg gaagaggaga cccgagtcat cttccgccag atcctgtctg
      481 cagttgcata tgtgcacagc cagggctatg cccacaggga cctcaagcca gaaaatttat
      541 tgtttgacga aaatcataag ctaaagctga ttgactttgg tctctgtgca aaacccaagg
      601 gcaacaagga ctaccatcta cagacatgct gtgggagtct ggcgtatgca gcacctgaac
      661 taatacaagg gaaatcgtat cttggatcag aggcagatgt ttggagcatg ggcatcctcc
      721 tatatgtgct tatgtgtgga ttcctcccgt ttgatgatga taatgtcatg gctttgtaca
      781 agaagataat gagagggaag tatgaagttc ccaagtggct gtctcccagc agcatcctgc
      841 ttctccagca gatgcttcag gtggacccaa agagacggat ttctatgaaa aatctcttga
      901 accatccctg gatcctgcaa gattacagct gtcccattga gtggcaaagc aaggcttctt
      961 tgactcacct tgatgaggat tgcataacag agctttctgt acatcaccgc aacagcaggc
     1021 aaacaatgga ggatttaatt tcgtcgtggc agtatgatca cctcacagcc acctaccttc
     1081 tgcttctagc caagaaggct cgagggaagc cagctcgtct gcagcttctg tccttctctg
     1141 gtgggaccac cagcaccact ccaaagtcaa agaacctgag cctggaagat atgtccacaa
     1201 gtggcgatag ctgtgtggct ggattaatag actatgaatt gtgtgaagat aatttactag
     1261 ctccccagac accacagttt accaaacact gggcagaatc aaatcatgtt gaatgtgaat
     1321 caccaactcc agtggtatgc agagcactgg ccaataagtt aaaggacaaa gagaatgtgt
     1381 gcactcccaa gtcttctgtg aagaatcaag agcaattcgt gttttctgag ccgaagattc
     1441 cagttagtcg gaaccagtat aagagagaaa tactcacctc accaacccag ttcacagcac
     1501 ctgcaaaagc tagaacgcag tgcctgagag aaacccaggt tagaacgccg gtgaattccg
     1561 caggagcaga cacgctaacg acaaatgtca ttagccctga gaggaggtgc cgttcaatgg
     1621 aagtggatct caaccaggca cacatggagg atacccctaa aaggaaagga accaatgtgt
     1681 ttgggagcct tgagagagga ctggataagg ttctcactgc acttacaagg aacaagaaga
     1741 agggctctgc caaagatggg ccaagaaggc gaaagctgca ctacaatgtg actacaacta
     1801 gactggtgaa tcccgaccag ctcctgaacg aaatcatggc tattcttcca aagaagaacg
     1861 tggacttcgt gcagaaaggt tacacactga agtgtcagac acagtctgat tttggcaaag
     1921 tgacaatgca gtttgaactg gaagtgtgcc agctgcagag acctgacgtg gtgggtatcc
     1981 ggagacagcg gctgaagggt gatgcctggg tttacaagag attagtggaa gatatcttgt
     2041 ctggctgcaa gatgtaaccg atggcggtgt ccatctaccc ggatgaatgt gggtgtggtg
     2101 ctgcacagaa gaagaccgtg gattggttgg attttcaggc ccgttggaac tacaggcgca
     2161 tttaaatcgc catcttcgag accaagacct ttttgttttt tcaaccaaag tttttacttt
     2221 gtggctgaat ccagggcagc tctcgtgttg ttatctatta ctgtctgtct tagtctgtgg
     2281 cttggggtgt taacaactgc tttctatatt cactcccatc tggcagtgcc agcccttccc
     2341 tgtgactcat tttgatgtgc agtttctttc tgaactaaaa ccatttgtga atatatcaag
     2401 cccttctttg tatctgattt tgatccaaat aaacctcgaa tggctttctg actgttaagt
     2461 aca
//
